UniProtKB - Q8WWW0 (RASF5_HUMAN)
Ras association domain-containing protein 5
RASSF5
Functioni
Caution
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 122 – 170 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- Ras GTPase binding Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- intracellular signal transduction Source: InterPro
- negative regulation of cell population proliferation Source: Ensembl
- positive regulation of protein ubiquitination Source: Ensembl
- regulation of apoptotic process Source: InterPro
- regulation of protein localization to nucleus Source: Ensembl
Keywordsi
Biological process | Apoptosis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8WWW0 |
SignaLinki | Q8WWW0 |
SIGNORi | Q8WWW0 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras association domain-containing protein 5Alternative name(s): New ras effector 1 Regulator for cell adhesion and polarization enriched in lymphoid tissues Short name: RAPL |
Gene namesi | Name:RASSF5 Synonyms:NORE1, RAPL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17609, RASSF5 |
MIMi | 607020, gene |
neXtProti | NX_Q8WWW0 |
VEuPathDBi | HostDB:ENSG00000266094.6 |
Subcellular locationi
Cytoskeleton
Other locations
Note: Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
Cytoskeleton
- microtubule Source: UniProtKB-KW
Nucleus
- nucleus Source: Ensembl
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 83593 |
OpenTargetsi | ENSG00000266094 |
PharmGKBi | PA134958571 |
Miscellaneous databases
Pharosi | Q8WWW0, Tbio |
Genetic variation databases
BioMutai | RASSF5 |
DMDMi | 74751587 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
ChainiPRO_0000240401 | 1 – 418 | Ras association domain-containing protein 5Add BLAST | 418 | ||
Isoform 2 (identifier: Q8WWW0-2) | |||||
Initiator methioninei | RemovedCombined sources |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 182 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 279 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 352 | PhosphothreonineCombined sources | 1 | ||
Isoform 2 (identifier: Q8WWW0-2) | |||||
Modified residuei | 2 | N-acetylthreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8WWW0 |
jPOSTi | Q8WWW0 |
MassIVEi | Q8WWW0 |
MaxQBi | Q8WWW0 |
PaxDbi | Q8WWW0 |
PeptideAtlasi | Q8WWW0 |
PRIDEi | Q8WWW0 |
ProteomicsDBi | 74944 [Q8WWW0-1] 74945 [Q8WWW0-2] 74946 [Q8WWW0-3] |
PTM databases
iPTMneti | Q8WWW0 |
PhosphoSitePlusi | Q8WWW0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000266094, Expressed in blood and 216 other tissues |
ExpressionAtlasi | Q8WWW0, baseline and differential |
Genevisiblei | Q8WWW0, HS |
Organism-specific databases
HPAi | ENSG00000266094, Tissue enhanced (blood, lymphoid tissue) |
Interactioni
Subunit structurei
Interacts directly with activated HRAS; a RASSF5-STK4/MST1 complex probably associates with activated HRAS (By similarity).
Interacts with KRAS (By similarity). Probably interacts with Ras-like GTPases RRAS, MRAS, RAP1B, RAP2A and RALA (By similarity).
Interacts with RRAS2 (PubMed:31130282). Can self-associate (By similarity).
Interacts with RSSF1 isoform A (By similarity). The RSSF1 isoform A-RSSF5 heterodimer probably mediates the association of RSSF1 with HRAS (By similarity).
Isoform 2 interacts with activated RAP1A and ITGAL/LFA-1 (PubMed:12845325, PubMed:15569673). Binds STK4/MST1, inhibiting STK4/MST1 autoactivation (PubMed:15109305).
By similarity4 PublicationsBinary interactionsi
Hide detailsQ8WWW0
RASSF5 - isoform 1 [Q8WWW0-1]
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-960496,EBI-960496 |
STK3 [Q13188] | 6 | EBI-960496,EBI-992580 |
STK4 - isoform 1 [Q13043-1] | 3 | EBI-960496,EBI-15638366 |
Isoform 2 [Q8WWW0-2]
Isoform 3 [Q8WWW0-3]
With | #Exp. | IntAct |
---|---|---|
STK4 [Q13043] | 2 | EBI-960507,EBI-367376 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- Ras GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 123689, 42 interactors |
DIPi | DIP-32490N |
IntActi | Q8WWW0, 51 interactors |
MINTi | Q8WWW0 |
STRINGi | 9606.ENSP00000462099 |
Miscellaneous databases
RNActi | Q8WWW0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8WWW0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 274 – 364 | Ras-associatingPROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 366 – 413 | SARAHPROSITE-ProRule annotationAdd BLAST | 48 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 122 – 170 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 49 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4239, Eukaryota |
GeneTreei | ENSGT00940000159288 |
HOGENOMi | CLU_045544_0_0_1 |
InParanoidi | Q8WWW0 |
OrthoDBi | 1120975at2759 |
PhylomeDBi | Q8WWW0 |
TreeFami | TF319243 |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
IDEALi | IID00631 |
InterProi | View protein in InterPro IPR033614, C-RASSF IPR002219, PE/DAG-bd IPR000159, RA_dom IPR033623, RASSF5 IPR011524, SARAH_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR22738, PTHR22738, 1 hit PTHR22738:SF9, PTHR22738:SF9, 1 hit |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF16517, Nore1-SARAH, 1 hit PF00788, RA, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00314, RA, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50200, RA, 1 hit PS50951, SARAH, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAMASPAIGQ RPYPLLLDPE PPRYLQSLSG PELPPPPPDR SSRLCVPAPL
60 70 80 90 100
STAPGAREGR SARRAARGNL EPPPRASRPA RPLRPGLQQR LRRRPGAPRP
110 120 130 140 150
RDVRSIFEQP QDPRVPAERG EGHCFAELVL PGGPGWCDLC GREVLRQALR
160 170 180 190 200
CTNCKFTCHP ECRSLIQLDC SQQEGLSRDR PSPESTLTVT FSQNVCKPVE
210 220 230 240 250
ETQRPPTLQE IKQKIDSYNT REKNCLGMKL SEDGTYTGFI KVHLKLRRPV
260 270 280 290 300
TVPAGIRPQS IYDAIKEVNL AATTDKRTSF YLPLDAIKQL HISSTTTVSE
310 320 330 340 350
VIQGLLKKFM VVDNPQKFAL FKRIHKDGQV LFQKLSIADR PLYLRLLAGP
360 370 380 390 400
DTEVLSFVLK ENETGEVEWD AFSIPELQNF LTILEKEEQD KIQQVQKKYD
410
KFRQKLEEAL RESQGKPG
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A075B763 | A0A075B763_HUMAN | Ras association domain-containing p... | RASSF5 | 350 | Annotation score: | ||
A0A1B0GTG4 | A0A1B0GTG4_HUMAN | Ras association domain-containing p... | RASSF5 | 318 | Annotation score: | ||
Q8TEK8 | Q8TEK8_HUMAN | FLJ00186 protein | RASSF5 FLJ00186 | 185 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019363 | 1 – 153 | Missing in isoform 2. 5 PublicationsAdd BLAST | 153 | |
Alternative sequenceiVSP_019364 | 154 – 193 | CKFTC…VTFSQ → MTVDSSMSSGYCSLDEELED CFFTAKTTFFRNAQSKHLSK in isoform 2. 5 PublicationsAdd BLAST | 40 | |
Alternative sequenceiVSP_019365 | 331 – 336 | LFQKLS → GCLLHP in isoform 3. 1 Publication | 6 | |
Alternative sequenceiVSP_019366 | 337 – 418 | Missing in isoform 3. 1 PublicationAdd BLAST | 82 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF445801 mRNA Translation: AAL38592.1 AY261332 mRNA Translation: AAP83360.1 AY062002 mRNA Translation: AAL40388.1 AY062003 mRNA Translation: AAL40389.1 AY216268 mRNA Translation: AAO61668.1 AK289861 mRNA Translation: BAF82550.1 AL832784 mRNA Translation: CAI46164.1 AL591846, AL354681 Genomic DNA Translation: CAI13536.1 AL591846, AL354681 Genomic DNA Translation: CAI13538.1 AL354681, AL591846 Genomic DNA Translation: CAI15252.1 AL591846, AL354681 Genomic DNA Translation: CAI13537.1 AL591846, AL354681 Genomic DNA Translation: CAI13542.1 AL354681, AL591846 Genomic DNA Translation: CAI15253.1 AL354681, AL591846 Genomic DNA Translation: CAI15254.1 AL354681, AL591846 Genomic DNA Translation: CAI15256.1 CH471100 Genomic DNA Translation: EAW93544.1 BC004270 mRNA Translation: AAH04270.1 Sequence problems. BC007203 mRNA Translation: AAH07203.1 Sequence problems. BC042651 mRNA Translation: AAH42651.1 |
CCDSi | CCDS1463.1 [Q8WWW0-3] CCDS1464.1 [Q8WWW0-2] CCDS30998.1 [Q8WWW0-1] |
RefSeqi | NP_872604.1, NM_182663.3 [Q8WWW0-1] NP_872605.1, NM_182664.3 [Q8WWW0-3] NP_872606.1, NM_182665.3 [Q8WWW0-2] |
Genome annotation databases
Ensembli | ENST00000577571; ENSP00000462576; ENSG00000266094 [Q8WWW0-2] ENST00000579436; ENSP00000462099; ENSG00000266094 [Q8WWW0-1] ENST00000580449; ENSP00000462544; ENSG00000266094 [Q8WWW0-3] |
GeneIDi | 83593 |
KEGGi | hsa:83593 |
UCSCi | uc031vlp.2, human [Q8WWW0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF445801 mRNA Translation: AAL38592.1 AY261332 mRNA Translation: AAP83360.1 AY062002 mRNA Translation: AAL40388.1 AY062003 mRNA Translation: AAL40389.1 AY216268 mRNA Translation: AAO61668.1 AK289861 mRNA Translation: BAF82550.1 AL832784 mRNA Translation: CAI46164.1 AL591846, AL354681 Genomic DNA Translation: CAI13536.1 AL591846, AL354681 Genomic DNA Translation: CAI13538.1 AL354681, AL591846 Genomic DNA Translation: CAI15252.1 AL591846, AL354681 Genomic DNA Translation: CAI13537.1 AL591846, AL354681 Genomic DNA Translation: CAI13542.1 AL354681, AL591846 Genomic DNA Translation: CAI15253.1 AL354681, AL591846 Genomic DNA Translation: CAI15254.1 AL354681, AL591846 Genomic DNA Translation: CAI15256.1 CH471100 Genomic DNA Translation: EAW93544.1 BC004270 mRNA Translation: AAH04270.1 Sequence problems. BC007203 mRNA Translation: AAH07203.1 Sequence problems. BC042651 mRNA Translation: AAH42651.1 |
CCDSi | CCDS1463.1 [Q8WWW0-3] CCDS1464.1 [Q8WWW0-2] CCDS30998.1 [Q8WWW0-1] |
RefSeqi | NP_872604.1, NM_182663.3 [Q8WWW0-1] NP_872605.1, NM_182664.3 [Q8WWW0-3] NP_872606.1, NM_182665.3 [Q8WWW0-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4LGD | X-ray | 3.05 | E/F/G/H | 365-413 | [»] | |
4OH8 | X-ray | 2.28 | B | 366-418 | [»] | |
SMRi | Q8WWW0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123689, 42 interactors |
DIPi | DIP-32490N |
IntActi | Q8WWW0, 51 interactors |
MINTi | Q8WWW0 |
STRINGi | 9606.ENSP00000462099 |
PTM databases
iPTMneti | Q8WWW0 |
PhosphoSitePlusi | Q8WWW0 |
Genetic variation databases
BioMutai | RASSF5 |
DMDMi | 74751587 |
Proteomic databases
EPDi | Q8WWW0 |
jPOSTi | Q8WWW0 |
MassIVEi | Q8WWW0 |
MaxQBi | Q8WWW0 |
PaxDbi | Q8WWW0 |
PeptideAtlasi | Q8WWW0 |
PRIDEi | Q8WWW0 |
ProteomicsDBi | 74944 [Q8WWW0-1] 74945 [Q8WWW0-2] 74946 [Q8WWW0-3] |
Protocols and materials databases
Antibodypediai | 73646, 266 antibodies |
DNASUi | 83593 |
Genome annotation databases
Ensembli | ENST00000577571; ENSP00000462576; ENSG00000266094 [Q8WWW0-2] ENST00000579436; ENSP00000462099; ENSG00000266094 [Q8WWW0-1] ENST00000580449; ENSP00000462544; ENSG00000266094 [Q8WWW0-3] |
GeneIDi | 83593 |
KEGGi | hsa:83593 |
UCSCi | uc031vlp.2, human [Q8WWW0-1] |
Organism-specific databases
CTDi | 83593 |
DisGeNETi | 83593 |
GeneCardsi | RASSF5 |
HGNCi | HGNC:17609, RASSF5 |
HPAi | ENSG00000266094, Tissue enhanced (blood, lymphoid tissue) |
MIMi | 607020, gene |
neXtProti | NX_Q8WWW0 |
OpenTargetsi | ENSG00000266094 |
PharmGKBi | PA134958571 |
VEuPathDBi | HostDB:ENSG00000266094.6 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4239, Eukaryota |
GeneTreei | ENSGT00940000159288 |
HOGENOMi | CLU_045544_0_0_1 |
InParanoidi | Q8WWW0 |
OrthoDBi | 1120975at2759 |
PhylomeDBi | Q8WWW0 |
TreeFami | TF319243 |
Enzyme and pathway databases
PathwayCommonsi | Q8WWW0 |
SignaLinki | Q8WWW0 |
SIGNORi | Q8WWW0 |
Miscellaneous databases
BioGRID-ORCSi | 83593, 4 hits in 875 CRISPR screens |
ChiTaRSi | RASSF5, human |
GeneWikii | RASSF5 |
GenomeRNAii | 83593 |
Pharosi | Q8WWW0, Tbio |
PROi | PR:Q8WWW0 |
RNActi | Q8WWW0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000266094, Expressed in blood and 216 other tissues |
ExpressionAtlasi | Q8WWW0, baseline and differential |
Genevisiblei | Q8WWW0, HS |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
IDEALi | IID00631 |
InterProi | View protein in InterPro IPR033614, C-RASSF IPR002219, PE/DAG-bd IPR000159, RA_dom IPR033623, RASSF5 IPR011524, SARAH_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR22738, PTHR22738, 1 hit PTHR22738:SF9, PTHR22738:SF9, 1 hit |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF16517, Nore1-SARAH, 1 hit PF00788, RA, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00314, RA, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50200, RA, 1 hit PS50951, SARAH, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RASF5_HUMAN | |
Accessioni | Q8WWW0Primary (citable) accession number: Q8WWW0 Secondary accession number(s): A8K1E6 Q9BT99 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references