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Protein

Ras association domain-containing protein 5

Gene

RASSF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.3 Publications

Caution

Was termed (Ref. 3) RASSF3.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri122 – 170Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • Ras GTPase binding Source: InterPro

GO - Biological processi

Keywordsi

Biological processApoptosis
LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ8WWW0
SIGNORiQ8WWW0

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 5
Alternative name(s):
New ras effector 1
Regulator for cell adhesion and polarization enriched in lymphoid tissues
Short name:
RAPL
Gene namesi
Name:RASSF5
Synonyms:NORE1, RAPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000266094.6
HGNCiHGNC:17609 RASSF5
MIMi607020 gene
neXtProtiNX_Q8WWW0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi83593
OpenTargetsiENSG00000266094
PharmGKBiPA134958571

Polymorphism and mutation databases

BioMutaiRASSF5
DMDMi74751587

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002404011 – 418Ras association domain-containing protein 5Add BLAST418
Isoform 2 (identifier: Q8WWW0-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182PhosphoserineBy similarity1
Modified residuei279PhosphoserineCombined sources1
Modified residuei352PhosphothreonineCombined sources1
Isoform 2 (identifier: Q8WWW0-2)
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WWW0
MaxQBiQ8WWW0
PaxDbiQ8WWW0
PeptideAtlasiQ8WWW0
PRIDEiQ8WWW0
ProteomicsDBi74944
74945 [Q8WWW0-2]
74946 [Q8WWW0-3]

PTM databases

iPTMnetiQ8WWW0
PhosphoSitePlusiQ8WWW0

Expressioni

Tissue specificityi

Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.2 Publications

Gene expression databases

BgeeiENSG00000266094 Expressed in 202 organ(s), highest expression level in blood
CleanExiHS_RASSF5
ExpressionAtlasiQ8WWW0 baseline and differential
GenevisibleiQ8WWW0 HS

Organism-specific databases

HPAiHPA070480

Interactioni

Subunit structurei

Interacts directly with activated HRAS; a RASSF5-STK4/MST1 complex probably associates with activated HRAS. Interacts with KRAS. Probably interacts with Ras-like GTPases RRAS, RRAS2, MRAS, RAP1B, RAP2A and RALA. Can self-associate. Interacts with RSSF1 isoform A. The RSSF1 isoform A-RSSF5 heterodimer probably mediates the association of RSSF1 with HRAS (By similarity). Isoform 2 interacts with activated RAP1A and ITGAL/LFA-1. Binds STK4/MST1, inhibiting STK4/MST1 autoactivation.By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123689, 41 interactors
DIPiDIP-32490N
IntActiQ8WWW0, 46 interactors
MINTiQ8WWW0
STRINGi9606.ENSP00000347443

Structurei

Secondary structure

1418
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8WWW0
SMRiQ8WWW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini274 – 364Ras-associatingPROSITE-ProRule annotationAdd BLAST91
Domaini366 – 413SARAHPROSITE-ProRule annotationAdd BLAST48

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri122 – 170Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IPAE Eukaryota
ENOG4111HS2 LUCA
GeneTreeiENSGT00390000003367
HOGENOMiHOG000013025
HOVERGENiHBG054362
InParanoidiQ8WWW0
KOiK08015
OrthoDBiEOG091G0EPB
PhylomeDBiQ8WWW0
TreeFamiTF319243

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR033614 C-RASSF
IPR002219 PE/DAG-bd
IPR000159 RA_dom
IPR033623 RASSF5
IPR011524 SARAH_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR22738 PTHR22738, 1 hit
PTHR22738:SF9 PTHR22738:SF9, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF16517 Nore1-SARAH, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00314 RA, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50951 SARAH, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8WWW0-1) [UniParc]FASTAAdd to basket
Also known as: A, NORE1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAMASPAIGQ RPYPLLLDPE PPRYLQSLSG PELPPPPPDR SSRLCVPAPL
60 70 80 90 100
STAPGAREGR SARRAARGNL EPPPRASRPA RPLRPGLQQR LRRRPGAPRP
110 120 130 140 150
RDVRSIFEQP QDPRVPAERG EGHCFAELVL PGGPGWCDLC GREVLRQALR
160 170 180 190 200
CTNCKFTCHP ECRSLIQLDC SQQEGLSRDR PSPESTLTVT FSQNVCKPVE
210 220 230 240 250
ETQRPPTLQE IKQKIDSYNT REKNCLGMKL SEDGTYTGFI KVHLKLRRPV
260 270 280 290 300
TVPAGIRPQS IYDAIKEVNL AATTDKRTSF YLPLDAIKQL HISSTTTVSE
310 320 330 340 350
VIQGLLKKFM VVDNPQKFAL FKRIHKDGQV LFQKLSIADR PLYLRLLAGP
360 370 380 390 400
DTEVLSFVLK ENETGEVEWD AFSIPELQNF LTILEKEEQD KIQQVQKKYD
410
KFRQKLEEAL RESQGKPG
Length:418
Mass (Da):47,090
Last modified:March 1, 2002 - v1
Checksum:i4AFBC69B1325CC9E
GO
Isoform 2 (identifier: Q8WWW0-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-153: Missing.
     154-193: CKFTCHPECR...ESTLTVTFSQ → MTVDSSMSSG...RNAQSKHLSK

Show »
Length:265
Mass (Da):30,359
Checksum:i66AD5D84EFA6D6CB
GO
Isoform 3 (identifier: Q8WWW0-3) [UniParc]FASTAAdd to basket
Also known as: C, NORE1B

The sequence of this isoform differs from the canonical sequence as follows:
     331-336: LFQKLS → GCLLHP
     337-418: Missing.

Show »
Length:336
Mass (Da):37,433
Checksum:i2657B6DAECD8D6A9
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A075B763A0A075B763_HUMAN
Ras association domain-containing p...
RASSF5
350Annotation score:
A0A1B0GTG4A0A1B0GTG4_HUMAN
Ras association domain-containing p...
RASSF5
318Annotation score:
Q8TEK8Q8TEK8_HUMAN
FLJ00186 protein
RASSF5 FLJ00186
185Annotation score:

Sequence cautioni

The sequence AAH04270 differs from that shown. Aberrant splicing.Curated
The sequence AAH04270 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH07203 differs from that shown. Aberrant splicing.Curated
The sequence AAH07203 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0193631 – 153Missing in isoform 2. 5 PublicationsAdd BLAST153
Alternative sequenceiVSP_019364154 – 193CKFTC…VTFSQ → MTVDSSMSSGYCSLDEELED CFFTAKTTFFRNAQSKHLSK in isoform 2. 5 PublicationsAdd BLAST40
Alternative sequenceiVSP_019365331 – 336LFQKLS → GCLLHP in isoform 3. 1 Publication6
Alternative sequenceiVSP_019366337 – 418Missing in isoform 3. 1 PublicationAdd BLAST82

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF445801 mRNA Translation: AAL38592.1
AY261332 mRNA Translation: AAP83360.1
AY062002 mRNA Translation: AAL40388.1
AY062003 mRNA Translation: AAL40389.1
AY216268 mRNA Translation: AAO61668.1
AK289861 mRNA Translation: BAF82550.1
AL832784 mRNA Translation: CAI46164.1
AL591846, AL354681 Genomic DNA Translation: CAI13536.1
AL591846, AL354681 Genomic DNA Translation: CAI13538.1
AL354681, AL591846 Genomic DNA Translation: CAI15252.1
AL591846, AL354681 Genomic DNA Translation: CAI13537.1
AL591846, AL354681 Genomic DNA Translation: CAI13542.1
AL354681, AL591846 Genomic DNA Translation: CAI15253.1
AL354681, AL591846 Genomic DNA Translation: CAI15254.1
AL354681, AL591846 Genomic DNA Translation: CAI15256.1
CH471100 Genomic DNA Translation: EAW93544.1
BC004270 mRNA Translation: AAH04270.1 Sequence problems.
BC007203 mRNA Translation: AAH07203.1 Sequence problems.
BC042651 mRNA Translation: AAH42651.1
CCDSiCCDS1463.1 [Q8WWW0-3]
CCDS1464.1 [Q8WWW0-2]
CCDS30998.1 [Q8WWW0-1]
RefSeqiNP_872604.1, NM_182663.3 [Q8WWW0-1]
NP_872605.1, NM_182664.3 [Q8WWW0-3]
NP_872606.1, NM_182665.3 [Q8WWW0-2]
UniGeneiHs.497579

Genome annotation databases

EnsembliENST00000577571; ENSP00000462576; ENSG00000266094 [Q8WWW0-2]
ENST00000579436; ENSP00000462099; ENSG00000266094 [Q8WWW0-1]
ENST00000580449; ENSP00000462544; ENSG00000266094 [Q8WWW0-3]
GeneIDi83593
KEGGihsa:83593
UCSCiuc031vlp.2 human [Q8WWW0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF445801 mRNA Translation: AAL38592.1
AY261332 mRNA Translation: AAP83360.1
AY062002 mRNA Translation: AAL40388.1
AY062003 mRNA Translation: AAL40389.1
AY216268 mRNA Translation: AAO61668.1
AK289861 mRNA Translation: BAF82550.1
AL832784 mRNA Translation: CAI46164.1
AL591846, AL354681 Genomic DNA Translation: CAI13536.1
AL591846, AL354681 Genomic DNA Translation: CAI13538.1
AL354681, AL591846 Genomic DNA Translation: CAI15252.1
AL591846, AL354681 Genomic DNA Translation: CAI13537.1
AL591846, AL354681 Genomic DNA Translation: CAI13542.1
AL354681, AL591846 Genomic DNA Translation: CAI15253.1
AL354681, AL591846 Genomic DNA Translation: CAI15254.1
AL354681, AL591846 Genomic DNA Translation: CAI15256.1
CH471100 Genomic DNA Translation: EAW93544.1
BC004270 mRNA Translation: AAH04270.1 Sequence problems.
BC007203 mRNA Translation: AAH07203.1 Sequence problems.
BC042651 mRNA Translation: AAH42651.1
CCDSiCCDS1463.1 [Q8WWW0-3]
CCDS1464.1 [Q8WWW0-2]
CCDS30998.1 [Q8WWW0-1]
RefSeqiNP_872604.1, NM_182663.3 [Q8WWW0-1]
NP_872605.1, NM_182664.3 [Q8WWW0-3]
NP_872606.1, NM_182665.3 [Q8WWW0-2]
UniGeneiHs.497579

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LGDX-ray3.05E/F/G/H365-413[»]
4OH8X-ray2.28B366-418[»]
ProteinModelPortaliQ8WWW0
SMRiQ8WWW0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123689, 41 interactors
DIPiDIP-32490N
IntActiQ8WWW0, 46 interactors
MINTiQ8WWW0
STRINGi9606.ENSP00000347443

PTM databases

iPTMnetiQ8WWW0
PhosphoSitePlusiQ8WWW0

Polymorphism and mutation databases

BioMutaiRASSF5
DMDMi74751587

Proteomic databases

EPDiQ8WWW0
MaxQBiQ8WWW0
PaxDbiQ8WWW0
PeptideAtlasiQ8WWW0
PRIDEiQ8WWW0
ProteomicsDBi74944
74945 [Q8WWW0-2]
74946 [Q8WWW0-3]

Protocols and materials databases

DNASUi83593
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000577571; ENSP00000462576; ENSG00000266094 [Q8WWW0-2]
ENST00000579436; ENSP00000462099; ENSG00000266094 [Q8WWW0-1]
ENST00000580449; ENSP00000462544; ENSG00000266094 [Q8WWW0-3]
GeneIDi83593
KEGGihsa:83593
UCSCiuc031vlp.2 human [Q8WWW0-1]

Organism-specific databases

CTDi83593
DisGeNETi83593
EuPathDBiHostDB:ENSG00000266094.6
GeneCardsiRASSF5
HGNCiHGNC:17609 RASSF5
HPAiHPA070480
MIMi607020 gene
neXtProtiNX_Q8WWW0
OpenTargetsiENSG00000266094
PharmGKBiPA134958571
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPAE Eukaryota
ENOG4111HS2 LUCA
GeneTreeiENSGT00390000003367
HOGENOMiHOG000013025
HOVERGENiHBG054362
InParanoidiQ8WWW0
KOiK08015
OrthoDBiEOG091G0EPB
PhylomeDBiQ8WWW0
TreeFamiTF319243

Enzyme and pathway databases

SignaLinkiQ8WWW0
SIGNORiQ8WWW0

Miscellaneous databases

ChiTaRSiRASSF5 human
GeneWikiiRASSF5
GenomeRNAii83593
PROiPR:Q8WWW0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000266094 Expressed in 202 organ(s), highest expression level in blood
CleanExiHS_RASSF5
ExpressionAtlasiQ8WWW0 baseline and differential
GenevisibleiQ8WWW0 HS

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR033614 C-RASSF
IPR002219 PE/DAG-bd
IPR000159 RA_dom
IPR033623 RASSF5
IPR011524 SARAH_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR22738 PTHR22738, 1 hit
PTHR22738:SF9 PTHR22738:SF9, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF16517 Nore1-SARAH, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00314 RA, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50951 SARAH, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRASF5_HUMAN
AccessioniPrimary (citable) accession number: Q8WWW0
Secondary accession number(s): A8K1E6
, Q5SY32, Q8WWV9, Q8WXF4, Q9BT99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2002
Last modified: September 12, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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