UniProtKB - Q8WWT9 (S13A3_HUMAN)
Protein
Solute carrier family 13 member 3
Gene
SLC13A3
Organism
Homo sapiens (Human)
Status
Functioni
High-affinity sodium-dicarboxylate cotransporter that accepts a range of substrates with 4-6 carbon atoms, including succinate, alpha-ketoglutarate and N-acetylaspartate (PubMed:30635937). The stoichiometry is probably 3 Na+ for 1 divalent succinate.1 Publication
GO - Molecular functioni
- alpha-ketoglutarate transmembrane transporter activity Source: UniProtKB
- citrate transmembrane transporter activity Source: ARUK-UCL
- dicarboxylic acid transmembrane transporter activity Source: ARUK-UCL
- glutathione transmembrane transporter activity Source: ARUK-UCL
- high-affinity sodium:dicarboxylate symporter activity Source: Ensembl
- sodium:dicarboxylate symporter activity Source: ARUK-UCL
- succinate transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- anion transmembrane transport Source: GO_Central
- citrate transport Source: ARUK-UCL
- dicarboxylic acid transport Source: ARUK-UCL
- glutathione transmembrane transport Source: ARUK-UCL
- succinate transmembrane transport Source: ARUK-UCL
- transport across blood-brain barrier Source: ARUK-UCL
Keywordsi
Biological process | Ion transport, Sodium transport, Symport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
PathwayCommonsi | Q8WWT9 |
Reactomei | R-HSA-433137, Sodium-coupled sulphate, di- and tri-carboxylate transporters |
SABIO-RKi | Q8WWT9 |
Protein family/group databases
TCDBi | 2.A.47.1.15, the divalent anion:na(+) symporter (dass) family |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 13 member 3Alternative name(s): Na(+)/dicarboxylate cotransporter 3 Short name: NaDC-3 Short name: hNaDC3 Sodium-dependent high-affinity dicarboxylate transporter 2 |
Gene namesi | Name:SLC13A3 Synonyms:NADC3, SDCT2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14430, SLC13A3 |
MIMi | 606411, gene |
neXtProti | NX_Q8WWT9 |
VEuPathDBi | HostDB:ENSG00000158296.13 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- basolateral plasma membrane Source: ARUK-UCL
- plasma membrane Source: HPA
Other locations
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 55 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 56 – 76 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 77 – 82 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 83 – 103 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 104 – 137 | ExtracellularSequence analysisAdd BLAST | 34 | |
Transmembranei | 138 – 158 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 159 – 229 | CytoplasmicSequence analysisAdd BLAST | 71 | |
Transmembranei | 230 – 250 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 251 – 278 | ExtracellularSequence analysisAdd BLAST | 28 | |
Transmembranei | 279 – 299 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 300 – 336 | CytoplasmicSequence analysisAdd BLAST | 37 | |
Transmembranei | 337 – 357 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 358 – 372 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 373 – 393 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 394 – 422 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Intramembranei | 423 – 443 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 444 – 461 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 462 – 482 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 483 – 505 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 506 – 526 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 527 – 546 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 547 – 567 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 568 – 602 | ExtracellularSequence analysisAdd BLAST | 35 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Leukoencephalopathy, acute reversible, with increased urinary alpha-ketoglutarate (ARLIAK)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive disorder characterized by acute, reversible neurological deterioration during febrile illness. Patients exhibit reversible leukoencephalopathy and increased urinary excretion of alpha-ketoglutarate.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_082121 | 254 | A → D in ARLIAK; unable to transport succinate, alpha-ketoglutarate and N-acetylaspartate. 1 PublicationCorresponds to variant dbSNP:rs1568927501EnsemblClinVar. | 1 | |
Natural variantiVAR_082122 | 548 | G → S in ARLIAK; severely decreased transport of succinate, alpha-ketoglutarate and N-acetylaspartate. 1 PublicationCorresponds to variant dbSNP:rs1568904872EnsemblClinVar. | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 64849 |
MalaCardsi | SLC13A3 |
MIMi | 618384, phenotype |
OpenTargetsi | ENSG00000158296 |
PharmGKBi | PA37881 |
Miscellaneous databases
Pharosi | Q8WWT9, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3712947 |
DrugBanki | DB00139, Succinic acid |
Genetic variation databases
BioMutai | SLC13A3 |
DMDMi | 23396845 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000172492 | 1 – 602 | Solute carrier family 13 member 3Add BLAST | 602 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 586 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 596 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q8WWT9 |
MassIVEi | Q8WWT9 |
MaxQBi | Q8WWT9 |
PaxDbi | Q8WWT9 |
PeptideAtlasi | Q8WWT9 |
PRIDEi | Q8WWT9 |
ProteomicsDBi | 28114 4325 74929 [Q8WWT9-1] 74930 [Q8WWT9-2] 74931 [Q8WWT9-3] 74932 [Q8WWT9-4] |
PTM databases
GlyGeni | Q8WWT9, 3 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q8WWT9 |
PhosphoSitePlusi | Q8WWT9 |
Expressioni
Tissue specificityi
Expression is highest in kidney. Detected in placenta, brain, liver and pancreas.
Gene expression databases
Bgeei | ENSG00000158296, Expressed in nephron tubule and 191 other tissues |
ExpressionAtlasi | Q8WWT9, baseline and differential |
Genevisiblei | Q8WWT9, HS |
Organism-specific databases
HPAi | ENSG00000158296, Tissue enriched (kidney) |
Interactioni
Binary interactionsi
Hide detailsQ8WWT9
Protein-protein interaction databases
BioGRIDi | 122322, 10 interactors |
IntActi | Q8WWT9, 10 interactors |
STRINGi | 9606.ENSP00000279027 |
Chemistry databases
BindingDBi | Q8WWT9 |
Miscellaneous databases
RNActi | Q8WWT9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1281, Eukaryota |
GeneTreei | ENSGT01000000214423 |
HOGENOMi | CLU_005170_9_1_1 |
InParanoidi | Q8WWT9 |
OMAi | IWWMTEA |
OrthoDBi | 389981at2759 |
PhylomeDBi | Q8WWT9 |
TreeFami | TF312913 |
Family and domain databases
InterProi | View protein in InterPro IPR001898, SLC13A/DASS |
Pfami | View protein in Pfam PF00939, Na_sulph_symp, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8WWT9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAALAAAAKK VWSARRLLVL LFTPLALLPV VFALPPKEGR CLFVILLMAV
60 70 80 90 100
YWCTEALPLS VTALLPIVLF PFMGILPSNK VCPQYFLDTN FLFLSGLIMA
110 120 130 140 150
SAIEEWNLHR RIALKILMLV GVQPARLILG MMVTTSFLSM WLSNTASTAM
160 170 180 190 200
MLPIANAILK SLFGQKEVRK DPSQESEENT AAVRRNGLHT VPTEMQFLAS
210 220 230 240 250
TEAKDHPGET EVPLDLPADS RKEDEYRRNI WKGFLISIPY SASIGGTATL
260 270 280 290 300
TGTAPNLILL GQLKSFFPQC DVVNFGSWFI FAFPLMLLFL LAGWLWISFL
310 320 330 340 350
YGGLSFRGWR KNKSEIRTNA EDRARAVIRE EYQNLGPIKF AEQAVFILFC
360 370 380 390 400
MFAILLFTRD PKFIPGWASL FNPGFLSDAV TGVAIVTILF FFPSQRPSLK
410 420 430 440 450
WWFDFKAPNT ETEPLLTWKK AQETVPWNII LLLGGGFAMA KGCEESGLSV
460 470 480 490 500
WIGGQLHPLE NVPPALAVLL ITVVIAFFTE FASNTATIII FLPVLAELAI
510 520 530 540 550
RLRVHPLYLM IPGTVGCSFA FMLPVSTPPN SIAFASGHLL VKDMVRTGLL
560 570 580 590 600
MNLMGVLLLS LAMNTWAQTI FQLGTFPDWA DMYSVNVTAL PPTLANDTFR
TL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J7L4 | C9J7L4_HUMAN | Solute carrier family 13 member 3 | SLC13A3 | 408 | Annotation score: | ||
A0A0A0MRQ6 | A0A0A0MRQ6_HUMAN | Solute carrier family 13 member 3 | SLC13A3 | 248 | Annotation score: | ||
X6RDV4 | X6RDV4_HUMAN | Solute carrier family 13 member 3 | SLC13A3 | 301 | Annotation score: | ||
C9J4A3 | C9J4A3_HUMAN | HCG2018530, isoform CRA_c | SLC13A3 hCG_2018530 | 185 | Annotation score: | ||
H7C0C8 | H7C0C8_HUMAN | Solute carrier family 13 member 3 | SLC13A3 | 236 | Annotation score: |
Sequence cautioni
The sequence AAF73251 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | L → P in BAC04834 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 364 | I → V in AAH35966 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 572 | Q → P in BAB71262 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_082121 | 254 | A → D in ARLIAK; unable to transport succinate, alpha-ketoglutarate and N-acetylaspartate. 1 PublicationCorresponds to variant dbSNP:rs1568927501EnsemblClinVar. | 1 | |
Natural variantiVAR_082122 | 548 | G → S in ARLIAK; severely decreased transport of succinate, alpha-ketoglutarate and N-acetylaspartate. 1 PublicationCorresponds to variant dbSNP:rs1568904872EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015291 | 1 – 47 | Missing in isoform 3 and isoform 6. 1 PublicationAdd BLAST | 47 | |
Alternative sequenceiVSP_006123 | 181 – 297 | AAVRR…GWLWI → GIEPNTFLSEERLKLQAPLV IRLGQITESGQWNMSGNDVC NFRVLSFLPGGM in isoform 2. CuratedAdd BLAST | 117 | |
Alternative sequenceiVSP_015292 | 182 – 292 | AVRRN…LFLLA → KTTLGRQRFHWICRLTPGRR MNIVGTSGRASSSPSPTQPV LGAQPHSRAQPLTSSCLASS R in isoform 4 and isoform 5. 1 PublicationAdd BLAST | 111 | |
Alternative sequenceiVSP_006124 | 298 – 602 | Missing in isoform 2. CuratedAdd BLAST | 305 | |
Alternative sequenceiVSP_015293 | 340 – 374 | Missing in isoform 3. 1 PublicationAdd BLAST | 35 | |
Alternative sequenceiVSP_015294 | 375 – 388 | FLSDA…AIVTI → CKMGIISISTIQKM in isoform 4. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_015295 | 389 – 602 | Missing in isoform 4. CuratedAdd BLAST | 214 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF154121 mRNA Translation: AAF73251.1 Frameshift. AY072810 mRNA Translation: AAL66762.1 AK056713 mRNA Translation: BAB71262.1 AK096658 mRNA Translation: BAC04834.1 AK295748 mRNA Translation: BAG58580.1 AL133520 Genomic DNA No translation available. AL034424 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW75725.1 CH471077 Genomic DNA Translation: EAW75726.1 BC035966 mRNA Translation: AAH35966.1 AL442082 mRNA Translation: CAC09447.2 Sequence problems. |
CCDSi | CCDS13400.1 [Q8WWT9-1] CCDS42886.1 [Q8WWT9-6] CCDS54469.1 [Q8WWT9-3] CCDS54470.1 [Q8WWT9-5] |
RefSeqi | NP_001011554.1, NM_001011554.2 [Q8WWT9-6] NP_001180268.1, NM_001193339.1 [Q8WWT9-5] NP_001180269.1, NM_001193340.1 [Q8WWT9-3] NP_073740.2, NM_022829.5 [Q8WWT9-1] |
Genome annotation databases
Ensembli | ENST00000279027; ENSP00000279027; ENSG00000158296 [Q8WWT9-1] ENST00000290317; ENSP00000290317; ENSG00000158296 [Q8WWT9-6] ENST00000413164; ENSP00000415852; ENSG00000158296 [Q8WWT9-5] ENST00000472148; ENSP00000420177; ENSG00000158296 [Q8WWT9-3] ENST00000495082; ENSP00000419621; ENSG00000158296 [Q8WWT9-6] |
GeneIDi | 64849 |
KEGGi | hsa:64849 |
UCSCi | uc002xsf.3, human [Q8WWT9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF154121 mRNA Translation: AAF73251.1 Frameshift. AY072810 mRNA Translation: AAL66762.1 AK056713 mRNA Translation: BAB71262.1 AK096658 mRNA Translation: BAC04834.1 AK295748 mRNA Translation: BAG58580.1 AL133520 Genomic DNA No translation available. AL034424 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW75725.1 CH471077 Genomic DNA Translation: EAW75726.1 BC035966 mRNA Translation: AAH35966.1 AL442082 mRNA Translation: CAC09447.2 Sequence problems. |
CCDSi | CCDS13400.1 [Q8WWT9-1] CCDS42886.1 [Q8WWT9-6] CCDS54469.1 [Q8WWT9-3] CCDS54470.1 [Q8WWT9-5] |
RefSeqi | NP_001011554.1, NM_001011554.2 [Q8WWT9-6] NP_001180268.1, NM_001193339.1 [Q8WWT9-5] NP_001180269.1, NM_001193340.1 [Q8WWT9-3] NP_073740.2, NM_022829.5 [Q8WWT9-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 122322, 10 interactors |
IntActi | Q8WWT9, 10 interactors |
STRINGi | 9606.ENSP00000279027 |
Chemistry databases
BindingDBi | Q8WWT9 |
ChEMBLi | CHEMBL3712947 |
DrugBanki | DB00139, Succinic acid |
Protein family/group databases
TCDBi | 2.A.47.1.15, the divalent anion:na(+) symporter (dass) family |
PTM databases
GlyGeni | Q8WWT9, 3 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q8WWT9 |
PhosphoSitePlusi | Q8WWT9 |
Genetic variation databases
BioMutai | SLC13A3 |
DMDMi | 23396845 |
Proteomic databases
jPOSTi | Q8WWT9 |
MassIVEi | Q8WWT9 |
MaxQBi | Q8WWT9 |
PaxDbi | Q8WWT9 |
PeptideAtlasi | Q8WWT9 |
PRIDEi | Q8WWT9 |
ProteomicsDBi | 28114 4325 74929 [Q8WWT9-1] 74930 [Q8WWT9-2] 74931 [Q8WWT9-3] 74932 [Q8WWT9-4] |
Protocols and materials databases
Antibodypediai | 13229, 127 antibodies |
DNASUi | 64849 |
Genome annotation databases
Ensembli | ENST00000279027; ENSP00000279027; ENSG00000158296 [Q8WWT9-1] ENST00000290317; ENSP00000290317; ENSG00000158296 [Q8WWT9-6] ENST00000413164; ENSP00000415852; ENSG00000158296 [Q8WWT9-5] ENST00000472148; ENSP00000420177; ENSG00000158296 [Q8WWT9-3] ENST00000495082; ENSP00000419621; ENSG00000158296 [Q8WWT9-6] |
GeneIDi | 64849 |
KEGGi | hsa:64849 |
UCSCi | uc002xsf.3, human [Q8WWT9-1] |
Organism-specific databases
CTDi | 64849 |
DisGeNETi | 64849 |
GeneCardsi | SLC13A3 |
HGNCi | HGNC:14430, SLC13A3 |
HPAi | ENSG00000158296, Tissue enriched (kidney) |
MalaCardsi | SLC13A3 |
MIMi | 606411, gene 618384, phenotype |
neXtProti | NX_Q8WWT9 |
OpenTargetsi | ENSG00000158296 |
PharmGKBi | PA37881 |
VEuPathDBi | HostDB:ENSG00000158296.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1281, Eukaryota |
GeneTreei | ENSGT01000000214423 |
HOGENOMi | CLU_005170_9_1_1 |
InParanoidi | Q8WWT9 |
OMAi | IWWMTEA |
OrthoDBi | 389981at2759 |
PhylomeDBi | Q8WWT9 |
TreeFami | TF312913 |
Enzyme and pathway databases
PathwayCommonsi | Q8WWT9 |
Reactomei | R-HSA-433137, Sodium-coupled sulphate, di- and tri-carboxylate transporters |
SABIO-RKi | Q8WWT9 |
Miscellaneous databases
BioGRID-ORCSi | 64849, 4 hits in 997 CRISPR screens |
ChiTaRSi | SLC13A3, human |
GeneWikii | SLC13A3 |
GenomeRNAii | 64849 |
Pharosi | Q8WWT9, Tbio |
PROi | PR:Q8WWT9 |
RNActi | Q8WWT9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158296, Expressed in nephron tubule and 191 other tissues |
ExpressionAtlasi | Q8WWT9, baseline and differential |
Genevisiblei | Q8WWT9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001898, SLC13A/DASS |
Pfami | View protein in Pfam PF00939, Na_sulph_symp, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S13A3_HUMAN | |
Accessioni | Q8WWT9Primary (citable) accession number: Q8WWT9 Secondary accession number(s): B4DIR8 Q9NS04 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2002 |
Last sequence update: | March 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families