UniProtKB - Q8WVK7 (SKA2_HUMAN)
Spindle and kinetochore-associated protein 2
SKA2
Functioni
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020).
Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495).
The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083).
The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083).
In the complex, it is required for SKA1 localization (PubMed:19289083).
Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).
3 PublicationsGO - Molecular functioni
- microtubule binding Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB
- chromosome segregation Source: UniProtKB
- mitotic cell cycle Source: UniProtKB
- regulation of microtubule polymerization or depolymerization Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q8WVK7 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q8WVK7 |
SIGNORi | Q8WVK7 |
Names & Taxonomyi
Protein namesi | Recommended name: Spindle and kinetochore-associated protein 2Alternative name(s): Protein FAM33A |
Gene namesi | Name:SKA2 Synonyms:FAM33A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28006, SKA2 |
MIMi | 616674, gene |
neXtProti | NX_Q8WVK7 |
VEuPathDBi | HostDB:ENSG00000182628 |
Subcellular locationi
Cytoskeleton
- spindle 2 Publications
Other locations
- kinetochore 2 Publications
Note: Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.2 Publications
Cytoskeleton
- spindle microtubule Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Other locations
- kinetochore Source: ComplexPortal
- outer kinetochore Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, MicrotubulePathology & Biotechi
Organism-specific databases
DisGeNETi | 348235 |
OpenTargetsi | ENSG00000182628 |
PharmGKBi | PA165432792 |
Miscellaneous databases
Pharosi | Q8WVK7, Tbio |
Genetic variation databases
BioMutai | SKA2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000266035 | 1 – 121 | Spindle and kinetochore-associated protein 2Add BLAST | 121 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 101 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8WVK7 |
jPOSTi | Q8WVK7 |
MassIVEi | Q8WVK7 |
MaxQBi | Q8WVK7 |
PaxDbi | Q8WVK7 |
PeptideAtlasi | Q8WVK7 |
PRIDEi | Q8WVK7 |
ProteomicsDBi | 19416 74801 [Q8WVK7-1] |
PTM databases
iPTMneti | Q8WVK7 |
PhosphoSitePlusi | Q8WVK7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000182628, Expressed in ventricular zone and 220 other tissues |
ExpressionAtlasi | Q8WVK7, baseline and differential |
Genevisiblei | Q8WVK7, HS |
Organism-specific databases
HPAi | ENSG00000182628, Low tissue specificity |
Interactioni
Subunit structurei
Component of the SKA1 complex, composed of SKA1, SKA2 and SKA3.
Forms a heterodimer with SKA1; the heterodimer interacting with SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers, each heterodimer interacting with a molecule of the SKA3 homodimer. The core SKA1 complex associates with microtubules and forms oligomeric assemblies.
Interacts directly with SKA1. Binds directly to microtubules; but with a much lower affinity than SKA1. May interact with NR3C1; the relevance of such interaction remains unclear in vivo.
2 PublicationsBinary interactionsi
Q8WVK7
With | #Exp. | IntAct |
---|---|---|
IFT57 [Q9NWB7] | 3 | EBI-1773994,EBI-725672 |
RALY [Q53GL6] | 3 | EBI-1773994,EBI-9512693 |
SKA1 [Q96BD8] | 11 | EBI-1773994,EBI-741854 |
GO - Molecular functioni
- microtubule binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 131515, 31 interactors |
ComplexPortali | CPX-5642, Kinetochore SKA complex |
IntActi | Q8WVK7, 15 interactors |
MINTi | Q8WVK7 |
STRINGi | 9606.ENSP00000333433 |
Miscellaneous databases
RNActi | Q8WVK7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8WVK7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S6SM, Eukaryota |
GeneTreei | ENSGT00390000009588 |
HOGENOMi | CLU_143881_0_0_1 |
InParanoidi | Q8WVK7 |
OMAi | YARFKPI |
OrthoDBi | 1529470at2759 |
PhylomeDBi | Q8WVK7 |
TreeFami | TF332958 |
Family and domain databases
InterProi | View protein in InterPro IPR026762, Ska2 IPR042091, Ska2_N |
PANTHERi | PTHR32017, PTHR32017, 1 hit |
Pfami | View protein in Pfam PF16740, SKA2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEAEVDKLEL MFQKAESDLD YIQYRLEYEI KTNHPDSASE KNPVTLLKEL
60 70 80 90 100
SVIKSRYQTL YARFKPVAVE QKESKSRICA TVKKTMNMIQ KLQKQTDLEL
110 120
SPLTKEEKTA AEQFKFHMPD L
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KSP0 | J3KSP0_HUMAN | Protein FAM33A | SKA2 | 109 | Annotation score: | ||
J3QL03 | J3QL03_HUMAN | Protein FAM33A | SKA2 | 66 | Annotation score: | ||
J3KTC5 | J3KTC5_HUMAN | Protein FAM33A | SKA2 | 92 | Annotation score: | ||
J3KRZ0 | J3KRZ0_HUMAN | Protein FAM33A | SKA2 | 62 | Annotation score: | ||
J3QS74 | J3QS74_HUMAN | Family with sequence similarity 33,... | SKA2 FAM33A, hCG_2000185 | 61 | Annotation score: | ||
J3QKN8 | J3QKN8_HUMAN | Family with sequence similarity 33,... | SKA2 FAM33A, hCG_2000185 | 78 | Annotation score: | ||
J3KRW9 | J3KRW9_HUMAN | Spindle and kinetochore-associated ... | SKA2 | 46 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047348 | 1 – 75 | MEAEV…QKESK → MASEVGHNLESPETPGGGGW TRVEFPPPAPKGAATVWCLN RLGSRKLSLIWITFNTGWNM KSRLIILIQQVSCHH in isoform 2. 1 PublicationAdd BLAST | 75 | |
Alternative sequenceiVSP_047349 | 76 – 121 | Missing in isoform 2. 1 PublicationAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK056473 mRNA Translation: BAG51725.1 AC099850 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94420.1 BC017873 mRNA Translation: AAH17873.1 BC106003 mRNA Translation: AAI06004.1 BI835364 mRNA No translation available. |
CCDSi | CCDS45747.1 [Q8WVK7-1] CCDS45748.1 [Q8WVK7-2] |
RefSeqi | NP_001094065.1, NM_001100595.1 [Q8WVK7-2] NP_872426.1, NM_182620.3 [Q8WVK7-1] |
Genome annotation databases
Ensembli | ENST00000330137; ENSP00000333433; ENSG00000182628 ENST00000437036; ENSP00000411231; ENSG00000182628 [Q8WVK7-2] |
GeneIDi | 348235 |
KEGGi | hsa:348235 |
MANE-Selecti | ENST00000330137.12; ENSP00000333433.7; NM_182620.4; NP_872426.1 |
UCSCi | uc002ixd.3, human [Q8WVK7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK056473 mRNA Translation: BAG51725.1 AC099850 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94420.1 BC017873 mRNA Translation: AAH17873.1 BC106003 mRNA Translation: AAI06004.1 BI835364 mRNA No translation available. |
CCDSi | CCDS45747.1 [Q8WVK7-1] CCDS45748.1 [Q8WVK7-2] |
RefSeqi | NP_001094065.1, NM_001100595.1 [Q8WVK7-2] NP_872426.1, NM_182620.3 [Q8WVK7-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AJ5 | X-ray | 3.32 | K/L/M/N/O/P/Q/R/S/T | 1-121 | [»] | |
SMRi | Q8WVK7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 131515, 31 interactors |
ComplexPortali | CPX-5642, Kinetochore SKA complex |
IntActi | Q8WVK7, 15 interactors |
MINTi | Q8WVK7 |
STRINGi | 9606.ENSP00000333433 |
PTM databases
iPTMneti | Q8WVK7 |
PhosphoSitePlusi | Q8WVK7 |
Genetic variation databases
BioMutai | SKA2 |
Proteomic databases
EPDi | Q8WVK7 |
jPOSTi | Q8WVK7 |
MassIVEi | Q8WVK7 |
MaxQBi | Q8WVK7 |
PaxDbi | Q8WVK7 |
PeptideAtlasi | Q8WVK7 |
PRIDEi | Q8WVK7 |
ProteomicsDBi | 19416 74801 [Q8WVK7-1] |
Protocols and materials databases
Antibodypediai | 31070, 136 antibodies from 24 providers |
DNASUi | 348235 |
Genome annotation databases
Ensembli | ENST00000330137; ENSP00000333433; ENSG00000182628 ENST00000437036; ENSP00000411231; ENSG00000182628 [Q8WVK7-2] |
GeneIDi | 348235 |
KEGGi | hsa:348235 |
MANE-Selecti | ENST00000330137.12; ENSP00000333433.7; NM_182620.4; NP_872426.1 |
UCSCi | uc002ixd.3, human [Q8WVK7-1] |
Organism-specific databases
CTDi | 348235 |
DisGeNETi | 348235 |
GeneCardsi | SKA2 |
HGNCi | HGNC:28006, SKA2 |
HPAi | ENSG00000182628, Low tissue specificity |
MIMi | 616674, gene |
neXtProti | NX_Q8WVK7 |
OpenTargetsi | ENSG00000182628 |
PharmGKBi | PA165432792 |
VEuPathDBi | HostDB:ENSG00000182628 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S6SM, Eukaryota |
GeneTreei | ENSGT00390000009588 |
HOGENOMi | CLU_143881_0_0_1 |
InParanoidi | Q8WVK7 |
OMAi | YARFKPI |
OrthoDBi | 1529470at2759 |
PhylomeDBi | Q8WVK7 |
TreeFami | TF332958 |
Enzyme and pathway databases
PathwayCommonsi | Q8WVK7 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q8WVK7 |
SIGNORi | Q8WVK7 |
Miscellaneous databases
BioGRID-ORCSi | 348235, 273 hits in 1053 CRISPR screens |
ChiTaRSi | SKA2, human |
GenomeRNAii | 348235 |
Pharosi | Q8WVK7, Tbio |
PROi | PR:Q8WVK7 |
RNActi | Q8WVK7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182628, Expressed in ventricular zone and 220 other tissues |
ExpressionAtlasi | Q8WVK7, baseline and differential |
Genevisiblei | Q8WVK7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026762, Ska2 IPR042091, Ska2_N |
PANTHERi | PTHR32017, PTHR32017, 1 hit |
Pfami | View protein in Pfam PF16740, SKA2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SKA2_HUMAN | |
Accessioni | Q8WVK7Primary (citable) accession number: Q8WVK7 Secondary accession number(s): A6NIL3, B3KPL3, E9PCB8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 12, 2006 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families