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Protein

Spindle and kinetochore-associated protein 2

Gene

SKA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).3 Publications

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB
  • chromosome segregation Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • regulation of microtubule polymerization or depolymerization Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle and kinetochore-associated protein 2
Alternative name(s):
Protein FAM33A
Gene namesi
Name:SKA2
Synonyms:FAM33A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000182628.12
HGNCiHGNC:28006 SKA2
MIMi616674 gene
neXtProtiNX_Q8WVK7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi348235
OpenTargetsiENSG00000182628
PharmGKBiPA165432792

Polymorphism and mutation databases

BioMutaiSKA2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002660351 – 121Spindle and kinetochore-associated protein 2Add BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WVK7
MaxQBiQ8WVK7
PaxDbiQ8WVK7
PeptideAtlasiQ8WVK7
PRIDEiQ8WVK7
ProteomicsDBi74801

PTM databases

iPTMnetiQ8WVK7
PhosphoSitePlusiQ8WVK7

Expressioni

Gene expression databases

BgeeiENSG00000182628 Expressed in 207 organ(s), highest expression level in forebrain
CleanExiHS_FAM33A
ExpressionAtlasiQ8WVK7 baseline and differential
GenevisibleiQ8WVK7 HS

Organism-specific databases

HPAiHPA059235

Interactioni

Subunit structurei

Component of the SKA1 complex, composed of SKA1, SKA2 and SKA3. Forms a heterodimer with SKA1; the heterodimer interacting with SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers, each heterodimer interacting with a molecule of the SKA3 homodimer. The core SKA1 complex associates with microtubules and forms oligomeric assemblies. Interacts directly with SKA1. Binds directly to microtubules; but with a much lower affinity than SKA1. May interact with NR3C1; the relevance of such interaction remains unclear in vivo.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SKA1Q96BD811EBI-1773994,EBI-741854

GO - Molecular functioni

Protein-protein interaction databases

BioGridi131515, 14 interactors
IntActiQ8WVK7, 12 interactors
MINTiQ8WVK7
STRINGi9606.ENSP00000333433

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8WVK7
SMRiQ8WVK7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SKA2 family.Curated

Phylogenomic databases

eggNOGiENOG410IZYA Eukaryota
ENOG4111NBU LUCA
GeneTreeiENSGT00390000009588
HOGENOMiHOG000154333
HOVERGENiHBG093956
InParanoidiQ8WVK7
OMAiKNRICAT
OrthoDBiEOG091G10JL
PhylomeDBiQ8WVK7
TreeFamiTF332958

Family and domain databases

InterProiView protein in InterPro
IPR026762 Ska2
PANTHERiPTHR32017 PTHR32017, 1 hit
PfamiView protein in Pfam
PF16740 SKA2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8WVK7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAEVDKLEL MFQKAESDLD YIQYRLEYEI KTNHPDSASE KNPVTLLKEL
60 70 80 90 100
SVIKSRYQTL YARFKPVAVE QKESKSRICA TVKKTMNMIQ KLQKQTDLEL
110 120
SPLTKEEKTA AEQFKFHMPD L
Length:121
Mass (Da):14,188
Last modified:March 1, 2002 - v1
Checksum:iD079F77E5BD4D7B0
GO
Isoform 2 (identifier: Q8WVK7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: MEAEVDKLEL...PVAVEQKESK → MASEVGHNLE...ILIQQVSCHH
     76-121: Missing.

Note: No experimental confirmation available.
Show »
Length:75
Mass (Da):8,282
Checksum:iBE3925745EDDA06B
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QS74J3QS74_HUMAN
Family with sequence similarity 33,...
SKA2 FAM33A, hCG_2000185
61Annotation score:
J3KSP0J3KSP0_HUMAN
Spindle and kinetochore-associated ...
SKA2
109Annotation score:
J3KRZ0J3KRZ0_HUMAN
Spindle and kinetochore-associated ...
SKA2
62Annotation score:
J3QL03J3QL03_HUMAN
Spindle and kinetochore-associated ...
SKA2
66Annotation score:
J3KTC5J3KTC5_HUMAN
Spindle and kinetochore-associated ...
SKA2
92Annotation score:
J3QKN8J3QKN8_HUMAN
Family with sequence similarity 33,...
SKA2 FAM33A, hCG_2000185
78Annotation score:
J3KRW9J3KRW9_HUMAN
Spindle and kinetochore-associated ...
SKA2
46Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0473481 – 75MEAEV…QKESK → MASEVGHNLESPETPGGGGW TRVEFPPPAPKGAATVWCLN RLGSRKLSLIWITFNTGWNM KSRLIILIQQVSCHH in isoform 2. 1 PublicationAdd BLAST75
Alternative sequenceiVSP_04734976 – 121Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056473 mRNA Translation: BAG51725.1
AC099850 Genomic DNA No translation available.
CH471109 Genomic DNA Translation: EAW94420.1
BC017873 mRNA Translation: AAH17873.1
BC106003 mRNA Translation: AAI06004.1
BI835364 mRNA No translation available.
CCDSiCCDS45747.1 [Q8WVK7-1]
CCDS45748.1 [Q8WVK7-2]
RefSeqiNP_001094065.1, NM_001100595.1 [Q8WVK7-2]
NP_872426.1, NM_182620.3 [Q8WVK7-1]
UniGeneiHs.463607

Genome annotation databases

EnsembliENST00000330137; ENSP00000333433; ENSG00000182628 [Q8WVK7-1]
ENST00000437036; ENSP00000411231; ENSG00000182628 [Q8WVK7-2]
GeneIDi348235
KEGGihsa:348235
UCSCiuc002ixd.3 human [Q8WVK7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056473 mRNA Translation: BAG51725.1
AC099850 Genomic DNA No translation available.
CH471109 Genomic DNA Translation: EAW94420.1
BC017873 mRNA Translation: AAH17873.1
BC106003 mRNA Translation: AAI06004.1
BI835364 mRNA No translation available.
CCDSiCCDS45747.1 [Q8WVK7-1]
CCDS45748.1 [Q8WVK7-2]
RefSeqiNP_001094065.1, NM_001100595.1 [Q8WVK7-2]
NP_872426.1, NM_182620.3 [Q8WVK7-1]
UniGeneiHs.463607

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AJ5X-ray3.32K/L/M/N/O/P/Q/R/S/T1-121[»]
ProteinModelPortaliQ8WVK7
SMRiQ8WVK7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131515, 14 interactors
IntActiQ8WVK7, 12 interactors
MINTiQ8WVK7
STRINGi9606.ENSP00000333433

PTM databases

iPTMnetiQ8WVK7
PhosphoSitePlusiQ8WVK7

Polymorphism and mutation databases

BioMutaiSKA2

Proteomic databases

EPDiQ8WVK7
MaxQBiQ8WVK7
PaxDbiQ8WVK7
PeptideAtlasiQ8WVK7
PRIDEiQ8WVK7
ProteomicsDBi74801

Protocols and materials databases

DNASUi348235
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330137; ENSP00000333433; ENSG00000182628 [Q8WVK7-1]
ENST00000437036; ENSP00000411231; ENSG00000182628 [Q8WVK7-2]
GeneIDi348235
KEGGihsa:348235
UCSCiuc002ixd.3 human [Q8WVK7-1]

Organism-specific databases

CTDi348235
DisGeNETi348235
EuPathDBiHostDB:ENSG00000182628.12
GeneCardsiSKA2
HGNCiHGNC:28006 SKA2
HPAiHPA059235
MIMi616674 gene
neXtProtiNX_Q8WVK7
OpenTargetsiENSG00000182628
PharmGKBiPA165432792
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZYA Eukaryota
ENOG4111NBU LUCA
GeneTreeiENSGT00390000009588
HOGENOMiHOG000154333
HOVERGENiHBG093956
InParanoidiQ8WVK7
OMAiKNRICAT
OrthoDBiEOG091G10JL
PhylomeDBiQ8WVK7
TreeFamiTF332958

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

ChiTaRSiSKA2 human
GenomeRNAii348235
PROiPR:Q8WVK7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182628 Expressed in 207 organ(s), highest expression level in forebrain
CleanExiHS_FAM33A
ExpressionAtlasiQ8WVK7 baseline and differential
GenevisibleiQ8WVK7 HS

Family and domain databases

InterProiView protein in InterPro
IPR026762 Ska2
PANTHERiPTHR32017 PTHR32017, 1 hit
PfamiView protein in Pfam
PF16740 SKA2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSKA2_HUMAN
AccessioniPrimary (citable) accession number: Q8WVK7
Secondary accession number(s): A6NIL3, B3KPL3, E9PCB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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