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Protein

RNA polymerase-associated protein LEO1

Gene

LEO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.5 Publications

GO - Molecular functioni

  • RNA polymerase II C-terminal domain phosphoserine binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase-associated protein LEO1
Alternative name(s):
Replicative senescence down-regulated leo1-like protein
Gene namesi
Name:LEO1
Synonyms:RDL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000166477.12
HGNCiHGNC:30401 LEO1
MIMi610507 gene
neXtProtiNX_Q8WVC0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi123169
OpenTargetsiENSG00000166477
PharmGKBiPA142671558

Polymorphism and mutation databases

BioMutaiLEO1
DMDMi74751545

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002478192 – 666RNA polymerase-associated protein LEO1Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei10PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei154PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei188PhosphothreonineCombined sources1
Modified residuei197PhosphoserineCombined sources1
Modified residuei205PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei229PhosphoserineCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei246PhosphoserineBy similarity1
Modified residuei254PhosphoserineBy similarity1
Modified residuei277PhosphoserineCombined sources1
Modified residuei279PhosphoserineCombined sources1
Modified residuei294PhosphoserineCombined sources1
Modified residuei296PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei606PhosphotyrosineCombined sources1
Modified residuei607PhosphoserineCombined sources1
Modified residuei608PhosphoserineCombined sources1
Modified residuei610PhosphoserineCombined sources1
Modified residuei614PhosphoserineCombined sources1
Modified residuei629PhosphothreonineCombined sources1
Modified residuei630PhosphoserineCombined sources1
Modified residuei658PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WVC0
MaxQBiQ8WVC0
PaxDbiQ8WVC0
PeptideAtlasiQ8WVC0
PRIDEiQ8WVC0
ProteomicsDBi74773
74774 [Q8WVC0-2]

PTM databases

iPTMnetiQ8WVC0
PhosphoSitePlusiQ8WVC0

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.1 Publication

Gene expression databases

BgeeiENSG00000166477 Expressed in 187 organ(s), highest expression level in amniotic fluid
CleanExiHS_LEO1
GenevisibleiQ8WVC0 HS

Organism-specific databases

HPAiHPA040741
HPA040941

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61 (PubMed:15632063, PubMed:19952111, PubMed:20178742). The PAF1 complex interacts with PHF5A (By similarity). Interacts with TCEA1, SUPT5H and CTNNB1 (PubMed:16630820, PubMed:19952111, PubMed:20178742). Interacts with SETD5 (By similarity).By similarity4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi125817, 206 interactors
CORUMiQ8WVC0
DIPiDIP-37885N
IntActiQ8WVC0, 39 interactors
STRINGi9606.ENSP00000299601

Structurei

Secondary structure

1666
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8WVC0
SMRiQ8WVC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 329Asp-richAdd BLAST327

Sequence similaritiesi

Belongs to the LEO1 family.Curated

Phylogenomic databases

eggNOGiKOG1181 Eukaryota
KOG2428 Eukaryota
ENOG410XRI0 LUCA
GeneTreeiENSGT00550000074952
HOGENOMiHOG000253934
HOVERGENiHBG081913
InParanoidiQ8WVC0
KOiK15177
OMAiDEKMQNS
OrthoDBiEOG091G0ILS
PhylomeDBiQ8WVC0
TreeFamiTF321961

Family and domain databases

InterProiView protein in InterPro
IPR007149 Leo1
PANTHERiPTHR23146 PTHR23146, 1 hit
PfamiView protein in Pfam
PF04004 Leo1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8WVC0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADMEDLFGS DADSEAERKD SDSGSDSDSD QENAASGSNA SGSESDQDER
60 70 80 90 100
GDSGQPSNKE LFGDDSEDEG ASHHSGSDNH SERSDNRSEA SERSDHEDND
110 120 130 140 150
PSDVDQHSGS EAPNDDEDEG HRSDGGSHHS EAEGSEKAHS DDEKWGREDK
160 170 180 190 200
SDQSDDEKIQ NSDDEERAQG SDEDKLQNSD DDEKMQNTDD EERPQLSDDE
210 220 230 240 250
RQQLSEEEKA NSDDERPVAS DNDDEKQNSD DEEQPQLSDE EKMQNSDDER
260 270 280 290 300
PQASDEEHRH SDDEEEQDHK SESARGSDSE DEVLRMKRKN AIASDSEADS
310 320 330 340 350
DTEVPKDNSG TMDLFGGADD ISSGSDGEDK PPTPGQPVDE NGLPQDQQEE
360 370 380 390 400
EPIPETRIEV EIPKVNTDLG NDLYFVKLPN FLSVEPRPFD PQYYEDEFED
410 420 430 440 450
EEMLDEEGRT RLKLKVENTI RWRIRRDEEG NEIKESNARI VKWSDGSMSL
460 470 480 490 500
HLGNEVFDVY KAPLQGDHNH LFIRQGTGLQ GQAVFKTKLT FRPHSTDSAT
510 520 530 540 550
HRKMTLSLAD RCSKTQKIRI LPMAGRDPEC QRTEMIKKEE ERLRASIRRE
560 570 580 590 600
SQQRRMREKQ HQRGLSASYL EPDRYDEEEE GEESISLAAI KNRYKGGIRE
610 620 630 640 650
ERARIYSSDS DEGSEEDKAQ RLLKAKKLTS DEEGEPSGKR KAEDDDKANK
660
KHKKYVISDE EEEDDD
Length:666
Mass (Da):75,404
Last modified:March 1, 2002 - v1
Checksum:iEB405BE0EDA7E0B0
GO
Isoform 2 (identifier: Q8WVC0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     387-446: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:606
Mass (Da):68,004
Checksum:iAF31C9793F9545D5
GO

Sequence cautioni

The sequence BAB71006 differs from that shown. Reason: Frameshift at position 473.Curated
Isoform 2 : The sequence BAB71006 differs from that shown. Reason: Frameshift at position 471.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020051387 – 446Missing in isoform 2. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302186 mRNA Translation: AAP68819.1
AK055762 mRNA Translation: BAB71006.1 Frameshift.
BC018147 mRNA Translation: AAH18147.1
CCDSiCCDS10146.1 [Q8WVC0-1]
CCDS66767.1 [Q8WVC0-2]
RefSeqiNP_001273359.1, NM_001286430.1 [Q8WVC0-2]
NP_620147.1, NM_138792.3 [Q8WVC0-1]
UniGeneiHs.567662

Genome annotation databases

EnsembliENST00000299601; ENSP00000299601; ENSG00000166477 [Q8WVC0-1]
ENST00000315141; ENSP00000314610; ENSG00000166477 [Q8WVC0-2]
GeneIDi123169
KEGGihsa:123169
UCSCiuc002abo.5 human [Q8WVC0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302186 mRNA Translation: AAP68819.1
AK055762 mRNA Translation: BAB71006.1 Frameshift.
BC018147 mRNA Translation: AAH18147.1
CCDSiCCDS10146.1 [Q8WVC0-1]
CCDS66767.1 [Q8WVC0-2]
RefSeqiNP_001273359.1, NM_001286430.1 [Q8WVC0-2]
NP_620147.1, NM_138792.3 [Q8WVC0-1]
UniGeneiHs.567662

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M6TX-ray2.50A370-462[»]
6GMHelectron microscopy3.10U1-666[»]
ProteinModelPortaliQ8WVC0
SMRiQ8WVC0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125817, 206 interactors
CORUMiQ8WVC0
DIPiDIP-37885N
IntActiQ8WVC0, 39 interactors
STRINGi9606.ENSP00000299601

PTM databases

iPTMnetiQ8WVC0
PhosphoSitePlusiQ8WVC0

Polymorphism and mutation databases

BioMutaiLEO1
DMDMi74751545

Proteomic databases

EPDiQ8WVC0
MaxQBiQ8WVC0
PaxDbiQ8WVC0
PeptideAtlasiQ8WVC0
PRIDEiQ8WVC0
ProteomicsDBi74773
74774 [Q8WVC0-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299601; ENSP00000299601; ENSG00000166477 [Q8WVC0-1]
ENST00000315141; ENSP00000314610; ENSG00000166477 [Q8WVC0-2]
GeneIDi123169
KEGGihsa:123169
UCSCiuc002abo.5 human [Q8WVC0-1]

Organism-specific databases

CTDi123169
DisGeNETi123169
EuPathDBiHostDB:ENSG00000166477.12
GeneCardsiLEO1
HGNCiHGNC:30401 LEO1
HPAiHPA040741
HPA040941
MIMi610507 gene
neXtProtiNX_Q8WVC0
OpenTargetsiENSG00000166477
PharmGKBiPA142671558
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1181 Eukaryota
KOG2428 Eukaryota
ENOG410XRI0 LUCA
GeneTreeiENSGT00550000074952
HOGENOMiHOG000253934
HOVERGENiHBG081913
InParanoidiQ8WVC0
KOiK15177
OMAiDEKMQNS
OrthoDBiEOG091G0ILS
PhylomeDBiQ8WVC0
TreeFamiTF321961

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins

Miscellaneous databases

ChiTaRSiLEO1 human
GeneWikiiLEO1
GenomeRNAii123169
PROiPR:Q8WVC0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166477 Expressed in 187 organ(s), highest expression level in amniotic fluid
CleanExiHS_LEO1
GenevisibleiQ8WVC0 HS

Family and domain databases

InterProiView protein in InterPro
IPR007149 Leo1
PANTHERiPTHR23146 PTHR23146, 1 hit
PfamiView protein in Pfam
PF04004 Leo1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLEO1_HUMAN
AccessioniPrimary (citable) accession number: Q8WVC0
Secondary accession number(s): Q96N99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
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