UniProtKB - Q8WUJ3 (CEMIP_HUMAN)
Cell migration-inducing and hyaluronan-binding protein
CEMIP
Functioni
Catalytic activityi
- Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.2 Publications EC:3.2.1.35
Activity regulationi
GO - Molecular functioni
- clathrin heavy chain binding Source: UniProtKB
- ER retention sequence binding Source: UniProtKB
- hyaluronic acid binding Source: UniProtKB
- hyalurononglucosaminidase activity Source: UniProtKB
GO - Biological processi
- hyaluronan biosynthetic process Source: Reactome
- hyaluronan catabolic process Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
- positive regulation of protein kinase C activity Source: UniProtKB
- positive regulation of protein targeting to membrane Source: UniProtKB
- positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
- sensory perception of sound Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Hyaluronic acid |
Enzyme and pathway databases
PathwayCommonsi | Q8WUJ3 |
Reactomei | R-HSA-2142850, Hyaluronan biosynthesis and export |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CEMIP Synonyms:KIAA1199 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29213, CEMIP |
MIMi | 608366, gene |
neXtProti | NX_Q8WUJ3 |
VEuPathDBi | HostDB:ENSG00000103888.15 |
Subcellular locationi
Extracellular region or secreted
Endoplasmic reticulum
Nucleus
Cytoplasm and Cytosol
Plasma membrane
Other locations
Note: Retained in the endoplasmic reticulum (ER) in a HSPA5/BIP-dependent manner. Colocalized with clathrin heavy chain/CLTC in clathrin-coated vesicles. Strongly detected in the cytoplasm of breast carcinoma cells, whereas poorly detected in adjacent normal epithelial cells, stromal cells, or benign breast tissues. Localized in the nucleus and cytoplasm of colon adenocarcinomas.
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: Reactome
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
- plasma membrane Source: UniProtKB
Other locations
- clathrin-coated endocytic vesicle Source: UniProtKB
- clathrin-coated vesicle membrane Source: Ensembl
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Coated pit, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 57214 |
OpenTargetsi | ENSG00000103888 |
PharmGKBi | PA134967531 |
Miscellaneous databases
Pharosi | Q8WUJ3, Tbio |
Genetic variation databases
BioMutai | CEMIP |
DMDMi | 46396475 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | 1 PublicationAdd BLAST | 30 | |
ChainiPRO_0000021538 | 31 – 1361 | Cell migration-inducing and hyaluronan-binding proteinAdd BLAST | 1331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 165 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 312 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 370 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 420 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 889 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 921 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q8WUJ3 |
MassIVEi | Q8WUJ3 |
PaxDbi | Q8WUJ3 |
PeptideAtlasi | Q8WUJ3 |
PRIDEi | Q8WUJ3 |
ProteomicsDBi | 74690 [Q8WUJ3-1] 74691 [Q8WUJ3-2] |
PTM databases
CarbonylDBi | Q8WUJ3 |
GlyGeni | Q8WUJ3, 7 sites |
iPTMneti | Q8WUJ3 |
PhosphoSitePlusi | Q8WUJ3 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000103888, Expressed in trigeminal ganglion and 169 other tissues |
ExpressionAtlasi | Q8WUJ3, baseline and differential |
Genevisiblei | Q8WUJ3, HS |
Organism-specific databases
HPAi | ENSG00000103888, Group enriched (endometrium, lymphoid tissue) |
Interactioni
Subunit structurei
Interacts with EPHA2 and ITPR3.
Interacts with HSPA5/BIP; the interaction induces calcium leakage from the endoplasmic reticulum and cell migration.
Interacts with clathrin heavy chain/CLTC.
3 PublicationsGO - Molecular functioni
- clathrin heavy chain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 121452, 3 interactors |
IntActi | Q8WUJ3, 3 interactors |
STRINGi | 9606.ENSP00000378177 |
Miscellaneous databases
RNActi | Q8WUJ3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 166 | G8PROSITE-ProRule annotationAdd BLAST | 123 | |
Repeati | 572 – 594 | PbH1 1Add BLAST | 23 | |
Repeati | 595 – 617 | PbH1 2Add BLAST | 23 | |
Repeati | 719 – 741 | PbH1 3Add BLAST | 23 | |
Repeati | 798 – 819 | PbH1 4Add BLAST | 22 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 295 – 591 | Necessary for its endoplasmic reticulum (ER) retention and interaction with HSPA51 PublicationAdd BLAST | 297 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QT0K, Eukaryota |
GeneTreei | ENSGT00940000153636 |
HOGENOMi | CLU_005606_1_0_1 |
InParanoidi | Q8WUJ3 |
OMAi | SRGPWTK |
PhylomeDBi | Q8WUJ3 |
TreeFami | TF316575 |
Family and domain databases
CDDi | cd13938, PANDER_like_TMEM2, 1 hit |
InterProi | View protein in InterPro IPR019316, G8_domain IPR039477, ILEI/PANDER_dom IPR011050, Pectin_lyase_fold/virulence IPR039473, TMEM2_PANDER-like IPR025155, WxxW_domain |
Pfami | View protein in Pfam PF10162, G8, 1 hit PF15711, ILEI, 2 hits PF13330, Mucin2_WxxW, 1 hit |
SMARTi | View protein in SMART SM01225, G8, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS51484, G8, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGAAGRQDFL FKAMLTISWL TLTCFPGATS TVAAGCPDQS PELQPWNPGH
60 70 80 90 100
DQDHHVHIGQ GKTLLLTSSA TVYSIHISEG GKLVIKDHDE PIVLRTRHIL
110 120 130 140 150
IDNGGELHAG SALCPFQGNF TIILYGRADE GIQPDPYYGL KYIGVGKGGA
160 170 180 190 200
LELHGQKKLS WTFLNKTLHP GGMAEGGYFF ERSWGHRGVI VHVIDPKSGT
210 220 230 240 250
VIHSDRFDTY RSKKESERLV QYLNAVPDGR ILSVAVNDEG SRNLDDMARK
260 270 280 290 300
AMTKLGSKHF LHLGFRHPWS FLTVKGNPSS SVEDHIEYHG HRGSAAARVF
310 320 330 340 350
KLFQTEHGEY FNVSLSSEWV QDVEWTEWFD HDKVSQTKGG EKISDLWKAH
360 370 380 390 400
PGKICNRPID IQATTMDGVN LSTEVVYKKG QDYRFACYDR GRACRSYRVR
410 420 430 440 450
FLCGKPVRPK LTVTIDTNVN STILNLEDNV QSWKPGDTLV IASTDYSMYQ
460 470 480 490 500
AEEFQVLPCR SCAPNQVKVA GKPMYLHIGE EIDGVDMRAE VGLLSRNIIV
510 520 530 540 550
MGEMEDKCYP YRNHICNFFD FDTFGGHIKF ALGFKAAHLE GTELKHMGQQ
560 570 580 590 600
LVGQYPIHFH LAGDVDERGG YDPPTYIRDL SIHHTFSRCV TVHGSNGLLI
610 620 630 640 650
KDVVGYNSLG HCFFTEDGPE ERNTFDHCLG LLVKSGTLLP SDRDSKMCKM
660 670 680 690 700
ITEDSYPGYI PKPRQDCNAV STFWMANPNN NLINCAAAGS EETGFWFIFH
710 720 730 740 750
HVPTGPSVGM YSPGYSEHIP LGKFYNNRAH SNYRAGMIID NGVKTTEASA
760 770 780 790 800
KDKRPFLSII SARYSPHQDA DPLKPREPAI IRHFIAYKNQ DHGAWLRGGD
810 820 830 840 850
VWLDSCRFAD NGIGLTLASG GTFPYDDGSK QEIKNSLFVG ESGNVGTEMM
860 870 880 890 900
DNRIWGPGGL DHSGRTLPIG QNFPIRGIQL YDGPINIQNC TFRKFVALEG
910 920 930 940 950
RHTSALAFRL NNAWQSCPHN NVTGIAFEDV PITSRVFFGE PGPWFNQLDM
960 970 980 990 1000
DGDKTSVFHD VDGSVSEYPG SYLTKNDNWL VRHPDCINVP DWRGAICSGC
1010 1020 1030 1040 1050
YAQMYIQAYK TSNLRMKIIK NDFPSHPLYL EGALTRSTHY QQYQPVVTLQ
1060 1070 1080 1090 1100
KGYTIHWDQT APAELAIWLI NFNKGDWIRV GLCYPRGTTF SILSDVHNRL
1110 1120 1130 1140 1150
LKQTSKTGVF VRTLQMDKVE QSYPGRSHYY WDEDSGLLFL KLKAQNEREK
1160 1170 1180 1190 1200
FAFCSMKGCE RIKIKALIPK NAGVSDCTAT AYPKFTERAV VDVPMPKKLF
1210 1220 1230 1240 1250
GSQLKTKDHF LEVKMESSKQ HFFHLWNDFA YIEVDGKKYP SSEDGIQVVV
1260 1270 1280 1290 1300
IDGNQGRVVS HTSFRNSILQ GIPWQLFNYV ATIPDNSIVL MASKGRYVSR
1310 1320 1330 1340 1350
GPWTRVLEKL GADRGLKLKE QMAFVGFKGS FRPIWVTLDT EDHKAKIFQV
1360
VPIPVVKKKK L
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YCE1 | H0YCE1_HUMAN | Cell migration-inducing and hyaluro... | CEMIP | 76 | Annotation score: | ||
H0YL56 | H0YL56_HUMAN | Cell migration-inducing and hyaluro... | CEMIP | 145 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 558 – 564 | HFHLAGD → TRPPTRP in CAB94391 (Ref. 6) Curated | 7 | |
Sequence conflicti | 862 | H → T in AAG41059 (PubMed:11247670).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_018165 | 187 | R → C in a family with non-syndromic hearing loss; reduces hyaluronic acid degradation activity. 2 PublicationsCorresponds to variant dbSNP:rs368854657Ensembl. | 1 | |
Natural variantiVAR_018166 | 187 | R → H in two unrelated families with non-syndromic hearing loss; reduces hyaluronic acid (HA) degradation activity. 2 PublicationsCorresponds to variant dbSNP:rs144446375Ensembl. | 1 | |
Natural variantiVAR_018167 | 783 | H → R Does not inhibit hyaluronic acid degradation activity. 2 PublicationsCorresponds to variant dbSNP:rs12441101EnsemblClinVar. | 1 | |
Natural variantiVAR_018168 | 783 | H → Y in a sporadic case of non-syndromic hearing loss. 1 PublicationCorresponds to variant dbSNP:rs996035812Ensembl. | 1 | |
Natural variantiVAR_018169 | 1109 | V → I Does not inhibit hyaluronic acid degradation activity. 2 PublicationsCorresponds to variant dbSNP:rs751807711Ensembl. | 1 | |
Natural variantiVAR_018170 | 1169 | P → A1 PublicationCorresponds to variant dbSNP:rs16972583Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009814 | 978 – 992 | NWLVR…NVPDW → KWHSLASKAASGPSG in isoform 2. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_009815 | 993 – 1361 | Missing in isoform 2. 1 PublicationAdd BLAST | 369 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB103330 mRNA Translation: BAD02451.1 CH471136 Genomic DNA Translation: EAW99111.1 BC020256 mRNA Translation: AAH20256.1 AB033025 mRNA Translation: BAA86513.1 AL359061 mRNA Translation: CAB94391.1 AY007811 mRNA Translation: AAG41059.1 |
CCDSi | CCDS10315.1 [Q8WUJ3-1] |
RefSeqi | NP_001280227.1, NM_001293298.1 [Q8WUJ3-1] NP_001280233.1, NM_001293304.1 [Q8WUJ3-1] NP_061159.1, NM_018689.2 [Q8WUJ3-1] |
Genome annotation databases
Ensembli | ENST00000220244; ENSP00000220244; ENSG00000103888 [Q8WUJ3-1] ENST00000356249; ENSP00000348583; ENSG00000103888 [Q8WUJ3-1] ENST00000394685; ENSP00000378177; ENSG00000103888 [Q8WUJ3-1] |
GeneIDi | 57214 |
KEGGi | hsa:57214 |
UCSCi | uc002bfw.2, human [Q8WUJ3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB103330 mRNA Translation: BAD02451.1 CH471136 Genomic DNA Translation: EAW99111.1 BC020256 mRNA Translation: AAH20256.1 AB033025 mRNA Translation: BAA86513.1 AL359061 mRNA Translation: CAB94391.1 AY007811 mRNA Translation: AAG41059.1 |
CCDSi | CCDS10315.1 [Q8WUJ3-1] |
RefSeqi | NP_001280227.1, NM_001293298.1 [Q8WUJ3-1] NP_001280233.1, NM_001293304.1 [Q8WUJ3-1] NP_061159.1, NM_018689.2 [Q8WUJ3-1] |
3D structure databases
SMRi | Q8WUJ3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121452, 3 interactors |
IntActi | Q8WUJ3, 3 interactors |
STRINGi | 9606.ENSP00000378177 |
PTM databases
CarbonylDBi | Q8WUJ3 |
GlyGeni | Q8WUJ3, 7 sites |
iPTMneti | Q8WUJ3 |
PhosphoSitePlusi | Q8WUJ3 |
Genetic variation databases
BioMutai | CEMIP |
DMDMi | 46396475 |
Proteomic databases
jPOSTi | Q8WUJ3 |
MassIVEi | Q8WUJ3 |
PaxDbi | Q8WUJ3 |
PeptideAtlasi | Q8WUJ3 |
PRIDEi | Q8WUJ3 |
ProteomicsDBi | 74690 [Q8WUJ3-1] 74691 [Q8WUJ3-2] |
Protocols and materials databases
Antibodypediai | 27890, 224 antibodies |
Genome annotation databases
Ensembli | ENST00000220244; ENSP00000220244; ENSG00000103888 [Q8WUJ3-1] ENST00000356249; ENSP00000348583; ENSG00000103888 [Q8WUJ3-1] ENST00000394685; ENSP00000378177; ENSG00000103888 [Q8WUJ3-1] |
GeneIDi | 57214 |
KEGGi | hsa:57214 |
UCSCi | uc002bfw.2, human [Q8WUJ3-1] |
Organism-specific databases
CTDi | 57214 |
DisGeNETi | 57214 |
GeneCardsi | CEMIP |
HGNCi | HGNC:29213, CEMIP |
HPAi | ENSG00000103888, Group enriched (endometrium, lymphoid tissue) |
MIMi | 608366, gene |
neXtProti | NX_Q8WUJ3 |
OpenTargetsi | ENSG00000103888 |
PharmGKBi | PA134967531 |
VEuPathDBi | HostDB:ENSG00000103888.15 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QT0K, Eukaryota |
GeneTreei | ENSGT00940000153636 |
HOGENOMi | CLU_005606_1_0_1 |
InParanoidi | Q8WUJ3 |
OMAi | SRGPWTK |
PhylomeDBi | Q8WUJ3 |
TreeFami | TF316575 |
Enzyme and pathway databases
PathwayCommonsi | Q8WUJ3 |
Reactomei | R-HSA-2142850, Hyaluronan biosynthesis and export |
Miscellaneous databases
BioGRID-ORCSi | 57214, 8 hits in 981 CRISPR screens |
ChiTaRSi | CEMIP, human |
GeneWikii | KIAA1199 |
GenomeRNAii | 57214 |
Pharosi | Q8WUJ3, Tbio |
PROi | PR:Q8WUJ3 |
RNActi | Q8WUJ3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103888, Expressed in trigeminal ganglion and 169 other tissues |
ExpressionAtlasi | Q8WUJ3, baseline and differential |
Genevisiblei | Q8WUJ3, HS |
Family and domain databases
CDDi | cd13938, PANDER_like_TMEM2, 1 hit |
InterProi | View protein in InterPro IPR019316, G8_domain IPR039477, ILEI/PANDER_dom IPR011050, Pectin_lyase_fold/virulence IPR039473, TMEM2_PANDER-like IPR025155, WxxW_domain |
Pfami | View protein in Pfam PF10162, G8, 1 hit PF15711, ILEI, 2 hits PF13330, Mucin2_WxxW, 1 hit |
SMARTi | View protein in SMART SM01225, G8, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS51484, G8, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CEMIP_HUMAN | |
Accessioni | Q8WUJ3Primary (citable) accession number: Q8WUJ3 Secondary accession number(s): Q6L9J5 Q9ULM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | April 13, 2004 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families