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Protein

Serine/threonine/tyrosine-interacting protein

Gene

STYX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytically inactive phosphatase (PubMed:23847209). Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2) (PubMed:23847209). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2) (PubMed:23847209). By binding to the F-box of FBXW7, prevents the assembly of FBXW7 into the SCF E3 ubiquitin-protein ligase complex, and thereby inhibits degradation of its substrates (PubMed:28007894). Plays a role in spermatogenesis (By similarity).By similarity2 Publications

Caution

Contains a Gly residue instead of a conserved Cys residue at position 120 in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and may protect them from phosphatases.1 Publication1 Publication

GO - Molecular functioni

  • F-box domain binding Source: UniProtKB
  • protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  • pseudophosphatase activity Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

SIGNORiQ8WUJ0

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine/tyrosine-interacting proteinCurated
Alternative name(s):
Inactive tyrosine-protein phosphatase STYXCurated
Phosphoserine/threonine/tyrosine interaction proteinBy similarity
Gene namesi
Name:STYXImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000198252.11
HGNCiHGNC:11447 STYX
MIMi615814 gene
neXtProtiNX_Q8WUJ0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76 – 78FQQ → AAA: Loss of interaction with FBXW7. Does not affect interaction with MAPK1 and nuclear localization. 1 Publication3
Mutagenesisi120G → C: Confers phosphatase activity. Dephosphorylates MAPK1. Does not affect interaction with FBXW7 and nuclear localization. 2 Publications1
Mutagenesisi142 – 144KYR → AAA: Increases interaction with FBXW7. 1 Publication3

Organism-specific databases

DisGeNETi6815
OpenTargetsiENSG00000198252
PharmGKBiPA36244

Polymorphism and mutation databases

BioMutaiSTYX
DMDMi29840812

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000949501 – 223Serine/threonine/tyrosine-interacting proteinAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184PhosphoserineCombined sources1
Modified residuei193PhosphoserineCombined sources1
Modified residuei201PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WUJ0
MaxQBiQ8WUJ0
PaxDbiQ8WUJ0
PeptideAtlasiQ8WUJ0
PRIDEiQ8WUJ0
ProteomicsDBi74688

PTM databases

DEPODiQ8WUJ0
iPTMnetiQ8WUJ0
PhosphoSitePlusiQ8WUJ0

Expressioni

Gene expression databases

BgeeiENSG00000198252 Expressed in 204 organ(s), highest expression level in liver
CleanExiHS_STYX
ExpressionAtlasiQ8WUJ0 baseline and differential
GenevisibleiQ8WUJ0 HS

Organism-specific databases

HPAiHPA040290

Interactioni

Subunit structurei

Interacts with MAPK1; independently of MAPK1 phosphorylation status (PubMed:23847209). Interacts with CARHSP1/Crhsp-24 (By similarity). Interacts (via FQQ motif) with FBXW7 isoforms 1 (via F-box domain) and 3 (via F-box domain); the interaction is direct and prevents FBXW7 interaction with SKP1, a component of the SCF(FBXW7) complex (PubMed:28007894). Does not interact with FBXW7 isoform 2 (PubMed:28007894).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112684, 69 interactors
STRINGi9606.ENSP00000346599

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8WUJ0
SMRiQ8WUJ0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WUJ0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 167Tyrosine-protein phosphataseAdd BLAST72

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 78Interaction with FBXW71 Publication3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000090240
HOVERGENiHBG044540
InParanoidiQ8WUJ0
KOiK18042
OMAiNVGFVHQ
OrthoDBiEOG091G0249
PhylomeDBiQ8WUJ0
TreeFamiTF350439

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequencei

Sequence statusi: Complete.

Q8WUJ0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEDVKLEFPS LPQCKEDAEE WTYPMRREMQ EILPGLFLGP YSSAMKSKLP
60 70 80 90 100
VLQKHGITHI ICIRQNIEAN FIKPNFQQLF RYLVLDIADN PVENIIRFFP
110 120 130 140 150
MTKEFIDGSL QMGGKVLVHG NAGISRSAAF VIAYIMETFG MKYRDAFAYV
160 170 180 190 200
QERRFCINPN AGFVHQLQEY EAIYLAKLTI QMMSPLQIER SLSVHSGTTG
210 220
SLKRTHEEED DFGTMQVATA QNG
Length:223
Mass (Da):25,492
Last modified:March 1, 2002 - v1
Checksum:iD2C8D05104073EE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098195 mRNA Translation: BAC05259.1
CH471078 Genomic DNA Translation: EAW65641.1
CH471078 Genomic DNA Translation: EAW65644.1
CH471061 Genomic DNA Translation: EAW80612.1
BC020265 mRNA Translation: AAH20265.1
BC146995 mRNA Translation: AAI46996.1
BC146998 mRNA Translation: AAI46999.1
U87169 mRNA Translation: AAB47561.1
CCDSiCCDS9711.1
RefSeqiNP_001124173.1, NM_001130701.1
NP_660294.1, NM_145251.3
UniGeneiHs.364980

Genome annotation databases

EnsembliENST00000354586; ENSP00000346599; ENSG00000198252
ENST00000442123; ENSP00000403214; ENSG00000198252
GeneIDi6815
KEGGihsa:6815
UCSCiuc001xaa.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098195 mRNA Translation: BAC05259.1
CH471078 Genomic DNA Translation: EAW65641.1
CH471078 Genomic DNA Translation: EAW65644.1
CH471061 Genomic DNA Translation: EAW80612.1
BC020265 mRNA Translation: AAH20265.1
BC146995 mRNA Translation: AAI46996.1
BC146998 mRNA Translation: AAI46999.1
U87169 mRNA Translation: AAB47561.1
CCDSiCCDS9711.1
RefSeqiNP_001124173.1, NM_001130701.1
NP_660294.1, NM_145251.3
UniGeneiHs.364980

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R0BX-ray1.60A26-177[»]
ProteinModelPortaliQ8WUJ0
SMRiQ8WUJ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112684, 69 interactors
STRINGi9606.ENSP00000346599

PTM databases

DEPODiQ8WUJ0
iPTMnetiQ8WUJ0
PhosphoSitePlusiQ8WUJ0

Polymorphism and mutation databases

BioMutaiSTYX
DMDMi29840812

Proteomic databases

EPDiQ8WUJ0
MaxQBiQ8WUJ0
PaxDbiQ8WUJ0
PeptideAtlasiQ8WUJ0
PRIDEiQ8WUJ0
ProteomicsDBi74688

Protocols and materials databases

DNASUi6815
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354586; ENSP00000346599; ENSG00000198252
ENST00000442123; ENSP00000403214; ENSG00000198252
GeneIDi6815
KEGGihsa:6815
UCSCiuc001xaa.4 human

Organism-specific databases

CTDi6815
DisGeNETi6815
EuPathDBiHostDB:ENSG00000198252.11
GeneCardsiSTYX
HGNCiHGNC:11447 STYX
HPAiHPA040290
MIMi615814 gene
neXtProtiNX_Q8WUJ0
OpenTargetsiENSG00000198252
PharmGKBiPA36244
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000090240
HOVERGENiHBG044540
InParanoidiQ8WUJ0
KOiK18042
OMAiNVGFVHQ
OrthoDBiEOG091G0249
PhylomeDBiQ8WUJ0
TreeFamiTF350439

Enzyme and pathway databases

SIGNORiQ8WUJ0

Miscellaneous databases

ChiTaRSiSTYX human
EvolutionaryTraceiQ8WUJ0
GenomeRNAii6815
PROiPR:Q8WUJ0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198252 Expressed in 204 organ(s), highest expression level in liver
CleanExiHS_STYX
ExpressionAtlasiQ8WUJ0 baseline and differential
GenevisibleiQ8WUJ0 HS

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTYX_HUMAN
AccessioniPrimary (citable) accession number: Q8WUJ0
Secondary accession number(s): B9EJG0, Q99850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: March 1, 2002
Last modified: September 12, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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