UniProtKB - Q8WUB8 (PHF10_HUMAN)
PHD finger protein 10
PHF10
Functioni
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
By similarityCaution
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 379 – 436 | PHD-type 1; degeneratePROSITE-ProRule annotationAdd BLAST | 58 | |
Zinc fingeri | 438 – 481 | PHD-type 2; degeneratePROSITE-ProRule annotationAdd BLAST | 44 |
GO - Molecular functioni
- histone binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- transcription coregulator activity Source: GO_Central
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: GO_Central
- nervous system development Source: UniProtKB-KW
- positive regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Biological process | Neurogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8WUB8 |
SignaLinki | Q8WUB8 |
Names & Taxonomyi
Protein namesi | Recommended name: PHD finger protein 10Alternative name(s): BRG1-associated factor 45a Short name: BAF45a XAP135 |
Gene namesi | Name:PHF10 Synonyms:BAF45A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18250, PHF10 |
MIMi | 613069, gene |
neXtProti | NX_Q8WUB8 |
VEuPathDBi | HostDB:ENSG00000130024 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- npBAF complex Source: UniProtKB
- nucleoplasm Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55274 |
OpenTargetsi | ENSG00000130024 |
PharmGKBi | PA134972675 |
Miscellaneous databases
Pharosi | Q8WUB8, Tbio |
Genetic variation databases
BioMutai | PHF10 |
DMDMi | 296439276 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000059297 | 2 – 498 | PHD finger protein 10Add BLAST | 497 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 36 | PhosphoserineCombined sources | 1 | |
Modified residuei | 50 | PhosphoserineCombined sources | 1 | |
Cross-linki | 241 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 270 | PhosphoserineCombined sources | 1 | |
Modified residuei | 297 | PhosphoserineCombined sources | 1 | |
Modified residuei | 301 | PhosphoserineCombined sources | 1 | |
Modified residuei | 327 | PhosphoserineCombined sources | 1 | |
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Cross-linki | 385 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8WUB8 |
jPOSTi | Q8WUB8 |
MassIVEi | Q8WUB8 |
MaxQBi | Q8WUB8 |
PaxDbi | Q8WUB8 |
PeptideAtlasi | Q8WUB8 |
PRIDEi | Q8WUB8 |
ProteomicsDBi | 74654 [Q8WUB8-1] 74655 [Q8WUB8-2] 74656 [Q8WUB8-3] |
PTM databases
iPTMneti | Q8WUB8 |
MetOSitei | Q8WUB8 |
PhosphoSitePlusi | Q8WUB8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000130024, Expressed in right adrenal gland and 239 other tissues |
ExpressionAtlasi | Q8WUB8, baseline and differential |
Genevisiblei | Q8WUB8, HS |
Organism-specific databases
HPAi | ENSG00000130024, Low tissue specificity |
Interactioni
Subunit structurei
Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin.
Interacts with ACTL6A/BAF53A, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A and PBRM1/BAF180 (By similarity).
By similarityBinary interactionsi
Isoform 2 [Q8WUB8-2]
With | #Exp. | IntAct |
---|---|---|
APP [P05067] | 3 | EBI-10276329,EBI-77613 |
L3MBTL2 [Q969R5] | 6 | EBI-10276329,EBI-739909 |
GO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 120562, 91 interactors |
ComplexPortali | CPX-1195, Embryonic stem cell-specific SWI/SNF ATP-dependent chromatin remodeling complex CPX-1196, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant CPX-1199, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6A variant CPX-1201, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant CPX-1212, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant CPX-1213, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant CPX-1215, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant |
CORUMi | Q8WUB8 |
IntActi | Q8WUB8, 43 interactors |
MINTi | Q8WUB8 |
STRINGi | 9606.ENSP00000341805 |
Miscellaneous databases
RNActi | Q8WUB8, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 62 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 89 – 295 | SAYAdd BLAST | 207 | |
Regioni | 89 – 185 | Essential to induce neural progenitor proliferationBy similarityAdd BLAST | 97 | |
Regioni | 285 – 368 | DisorderedSequence analysisAdd BLAST | 84 | |
Regioni | 292 – 334 | Essential to induce neural progenitor proliferationBy similarityAdd BLAST | 43 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 42 – 62 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 319 – 339 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 340 – 361 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 379 – 436 | PHD-type 1; degeneratePROSITE-ProRule annotationAdd BLAST | 58 | |
Zinc fingeri | 438 – 481 | PHD-type 2; degeneratePROSITE-ProRule annotationAdd BLAST | 44 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1512, Eukaryota |
GeneTreei | ENSGT00940000155172 |
HOGENOMi | CLU_028634_2_0_1 |
InParanoidi | Q8WUB8 |
OMAi | TEYKWPA |
OrthoDBi | 708781at2759 |
PhylomeDBi | Q8WUB8 |
TreeFami | TF318971 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038045, PHF10 IPR011011, Znf_FYVE_PHD IPR001965, Znf_PHD IPR019787, Znf_PHD-finger IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR10615:SF174, PTHR10615:SF174, 1 hit |
Pfami | View protein in Pfam PF00628, PHD, 1 hit |
SMARTi | View protein in SMART SM00249, PHD, 2 hits |
SUPFAMi | SSF57903, SSF57903, 2 hits |
PROSITEi | View protein in PROSITE PS01359, ZF_PHD_1, 1 hit PS50016, ZF_PHD_2, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAAGPGAA LSPRPCDSDP ATPGAQSPKD DNEDNSNDGT QPSKRRRMGS
60 70 80 90 100
GDSSRSCETS SQDLGFSYYP AENLIEYKWP PDETGEYYML QEQVSEYLGV
110 120 130 140 150
TSFKRKYPDL ERRDLSHKEK LYLRELNVIT ETQCTLGLTA LRSDEVIDLM
160 170 180 190 200
IKEYPAKHAE YSVILQEKER QRITDHYKEY SQMQQQNTQK VEASKVPEYI
210 220 230 240 250
KKAAKKAAEF NSNLNRERME ERRAYFDLQT HVIQVPQGKY KVLPTERTKV
260 270 280 290 300
SSYPVALIPG QFQEYYKRYS PDELRYLPLN TALYEPPLDP ELPALDSDGD
310 320 330 340 350
SDDGEDGRGD EKRKNKGTSD SSSGNVSEGE SPPDSQEDSF QGRQKSKDKA
360 370 380 390 400
ATPRKDGPKR SVLSKSVPGY KPKVIPNAIC GICLKGKESN KKGKAESLIH
410 420 430 440 450
CSQCENSGHP SCLDMTMELV SMIKTYPWQC MECKTCIICG QPHHEEEMMF
460 470 480 490
CDMCDRGYHT FCVGLGAIPS GRWICDCCQR APPTPRKVGR RGKNSKEG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketS5FZ81 | S5FZ81_HUMAN | PHD finger protein 10 | PHF10 | 377 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 126 | L → P in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 246 | E → G in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 275 | R → W in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 286 – 287 | PP → AT in AAK27451 (PubMed:11827455).Curated | 2 | |
Sequence conflicti | 325 | N → K in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 332 | P → S in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 357 | G → A in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 365 | K → N in AAK27451 (PubMed:11827455).Curated | 1 | |
Sequence conflicti | 422 | M → T in BAD96332 (Ref. 7) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039090 | 1 – 47 | Missing in isoform 3. 1 PublicationAdd BLAST | 47 | |
Alternative sequenceiVSP_013440 | 109 – 110 | Missing in isoform 2. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513547 Genomic DNA No translation available. BC020954 mRNA Translation: AAH20954.1 Different initiation. BC110323 mRNA Translation: AAI10324.1 Different initiation. AF338735 Genomic DNA Translation: AAK27451.1 Sequence problems. AK001837 mRNA Translation: BAA91934.1 Different initiation. CR457273 mRNA Translation: CAG33554.1 Different initiation. AK222612 mRNA Translation: BAD96332.1 Different initiation. |
CCDSi | CCDS5308.2 [Q8WUB8-1] CCDS5309.2 [Q8WUB8-2] |
RefSeqi | NP_060758.2, NM_018288.3 [Q8WUB8-1] NP_579866.2, NM_133325.2 [Q8WUB8-2] |
Genome annotation databases
Ensembli | ENST00000339209; ENSP00000341805; ENSG00000130024 ENST00000366780; ENSP00000355743; ENSG00000130024 [Q8WUB8-2] ENST00000621772; ENSP00000484117; ENSG00000130024 [Q8WUB8-3] |
GeneIDi | 55274 |
KEGGi | hsa:55274 |
MANE-Selecti | ENST00000339209.9; ENSP00000341805.4; NM_018288.4; NP_060758.2 |
UCSCi | uc011egy.3, human [Q8WUB8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513547 Genomic DNA No translation available. BC020954 mRNA Translation: AAH20954.1 Different initiation. BC110323 mRNA Translation: AAI10324.1 Different initiation. AF338735 Genomic DNA Translation: AAK27451.1 Sequence problems. AK001837 mRNA Translation: BAA91934.1 Different initiation. CR457273 mRNA Translation: CAG33554.1 Different initiation. AK222612 mRNA Translation: BAD96332.1 Different initiation. |
CCDSi | CCDS5308.2 [Q8WUB8-1] CCDS5309.2 [Q8WUB8-2] |
RefSeqi | NP_060758.2, NM_018288.3 [Q8WUB8-1] NP_579866.2, NM_133325.2 [Q8WUB8-2] |
3D structure databases
SMRi | Q8WUB8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120562, 91 interactors |
ComplexPortali | CPX-1195, Embryonic stem cell-specific SWI/SNF ATP-dependent chromatin remodeling complex CPX-1196, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant CPX-1199, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6A variant CPX-1201, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant CPX-1212, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant CPX-1213, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant CPX-1215, Neural progenitor-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant |
CORUMi | Q8WUB8 |
IntActi | Q8WUB8, 43 interactors |
MINTi | Q8WUB8 |
STRINGi | 9606.ENSP00000341805 |
PTM databases
iPTMneti | Q8WUB8 |
MetOSitei | Q8WUB8 |
PhosphoSitePlusi | Q8WUB8 |
Genetic variation databases
BioMutai | PHF10 |
DMDMi | 296439276 |
Proteomic databases
EPDi | Q8WUB8 |
jPOSTi | Q8WUB8 |
MassIVEi | Q8WUB8 |
MaxQBi | Q8WUB8 |
PaxDbi | Q8WUB8 |
PeptideAtlasi | Q8WUB8 |
PRIDEi | Q8WUB8 |
ProteomicsDBi | 74654 [Q8WUB8-1] 74655 [Q8WUB8-2] 74656 [Q8WUB8-3] |
Protocols and materials databases
Antibodypediai | 33573, 88 antibodies from 20 providers |
DNASUi | 55274 |
Genome annotation databases
Ensembli | ENST00000339209; ENSP00000341805; ENSG00000130024 ENST00000366780; ENSP00000355743; ENSG00000130024 [Q8WUB8-2] ENST00000621772; ENSP00000484117; ENSG00000130024 [Q8WUB8-3] |
GeneIDi | 55274 |
KEGGi | hsa:55274 |
MANE-Selecti | ENST00000339209.9; ENSP00000341805.4; NM_018288.4; NP_060758.2 |
UCSCi | uc011egy.3, human [Q8WUB8-1] |
Organism-specific databases
CTDi | 55274 |
DisGeNETi | 55274 |
GeneCardsi | PHF10 |
HGNCi | HGNC:18250, PHF10 |
HPAi | ENSG00000130024, Low tissue specificity |
MIMi | 613069, gene |
neXtProti | NX_Q8WUB8 |
OpenTargetsi | ENSG00000130024 |
PharmGKBi | PA134972675 |
VEuPathDBi | HostDB:ENSG00000130024 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1512, Eukaryota |
GeneTreei | ENSGT00940000155172 |
HOGENOMi | CLU_028634_2_0_1 |
InParanoidi | Q8WUB8 |
OMAi | TEYKWPA |
OrthoDBi | 708781at2759 |
PhylomeDBi | Q8WUB8 |
TreeFami | TF318971 |
Enzyme and pathway databases
PathwayCommonsi | Q8WUB8 |
SignaLinki | Q8WUB8 |
Miscellaneous databases
BioGRID-ORCSi | 55274, 27 hits in 1047 CRISPR screens |
ChiTaRSi | PHF10, human |
GeneWikii | PHF10 |
GenomeRNAii | 55274 |
Pharosi | Q8WUB8, Tbio |
PROi | PR:Q8WUB8 |
RNActi | Q8WUB8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000130024, Expressed in right adrenal gland and 239 other tissues |
ExpressionAtlasi | Q8WUB8, baseline and differential |
Genevisiblei | Q8WUB8, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038045, PHF10 IPR011011, Znf_FYVE_PHD IPR001965, Znf_PHD IPR019787, Znf_PHD-finger IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR10615:SF174, PTHR10615:SF174, 1 hit |
Pfami | View protein in Pfam PF00628, PHD, 1 hit |
SMARTi | View protein in SMART SM00249, PHD, 2 hits |
SUPFAMi | SSF57903, SSF57903, 2 hits |
PROSITEi | View protein in PROSITE PS01359, ZF_PHD_1, 1 hit PS50016, ZF_PHD_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PHF10_HUMAN | |
Accessioni | Q8WUB8Primary (citable) accession number: Q8WUB8 Secondary accession number(s): Q2YDA3 Q9NV26 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 23, 2022 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families