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Protein

Rhodopsin kinase

Gene

GRK7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).1 Publication

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Catalytic activityi

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.1 Publication

Activity regulationi

Inhibited by phosphorylation of Ser-36.By similarity

Kineticsi

  1. KM=1.9 µM for rhodopsin1 Publication
  2. KM=21.4 µM for ATP1 Publication
  1. Vmax=915 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei316Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • G protein-coupled receptor kinase activity Source: InterPro
  • rhodopsin kinase activity Source: UniProtKB

GO - Biological processi

  • protein autophosphorylation Source: UniProtKB
  • regulation of rhodopsin mediated signaling pathway Source: Reactome
  • signal transduction Source: InterPro
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processSensory transduction, Vision
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14 2681
ReactomeiR-HSA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
SignaLinkiQ8WTQ7

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin kinase (EC:2.7.11.141 Publication)
Alternative name(s):
G protein-coupled receptor kinase 7
G protein-coupled receptor kinase GRK7
Gene namesi
Name:GRK7
Synonyms:GPRK7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114124.2
HGNCiHGNC:17031 GRK7
MIMi606987 gene
neXtProtiNX_Q8WTQ7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23S → A: No effect on kinase activity. Increase kinase activity; when associated with A-36. 1 Publication1
Mutagenesisi36S → A: No effect on kinase activity. Increase kinase activity; when associated with A-23. 1 Publication1

Organism-specific databases

DisGeNETi131890
OpenTargetsiENSG00000114124
PharmGKBiPA38433

Chemistry databases

ChEMBLiCHEMBL1075133
GuidetoPHARMACOLOGYi1471

Polymorphism and mutation databases

BioMutaiGRK7
DMDMi21263659

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000243321 – 550Rhodopsin kinaseAdd BLAST550
PropeptideiPRO_0000024333551 – 553Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36Phosphoserine; by PKA1 Publication1
Modified residuei550Cysteine methyl esterSequence analysis1
Lipidationi550S-geranylgeranyl cysteineSequence analysis1

Post-translational modificationi

Autophosphorylated in vitro at Ser-490. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ8WTQ7
PeptideAtlasiQ8WTQ7
PRIDEiQ8WTQ7
ProteomicsDBi74583

PTM databases

iPTMnetiQ8WTQ7
PhosphoSitePlusiQ8WTQ7

Expressioni

Tissue specificityi

Retinal cones, outer and inner segments.2 Publications

Gene expression databases

BgeeiENSG00000114124 Expressed in 20 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_GRK7
GenevisibleiQ8WTQ7 HS

Interactioni

Subunit structurei

Interacts (when prenylated) with PDE6D; this promotes release from membranes.By similarity

Protein-protein interaction databases

BioGridi126297, 2 interactors
IntActiQ8WTQ7, 1 interactor
STRINGi9606.ENSP00000264952

Chemistry databases

BindingDBiQ8WTQ7

Structurei

3D structure databases

ProteinModelPortaliQ8WTQ7
SMRiQ8WTQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 176RGSPROSITE-ProRule annotationAdd BLAST121
Domaini191 – 454Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini455 – 520AGC-kinase C-terminalAdd BLAST66

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG004532
InParanoidiQ8WTQ7
KOiK00909
OMAiRTPFKDY
OrthoDBiEOG091G062G
PhylomeDBiQ8WTQ7
TreeFamiTF313940

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WTQ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVDMGALDNL IANTAYLQAR KPSDCDSKEL QRRRRSLALP GLQGCAELRQ
60 70 80 90 100
KLSLNFHSLC EQQPIGRRLF RDFLATVPTF RKAATFLEDV QNWELAEEGP
110 120 130 140 150
TKDSALQGLV ATCASAPAPG NPQPFLSQAV ATKCQAATTE EERVAAVTLA
160 170 180 190 200
KAEAMAFLQE QPFKDFVTSA FYDKFLQWKL FEMQPVSDKY FTEFRVLGKG
210 220 230 240 250
GFGEVCAVQV KNTGKMYACK KLDKKRLKKK GGEKMALLEK EILEKVSSPF
260 270 280 290 300
IVSLAYAFES KTHLCLVMSL MNGGDLKFHI YNVGTRGLDM SRVIFYSAQI
310 320 330 340 350
ACGMLHLHEL GIVYRDMKPE NVLLDDLGNC RLSDLGLAVE MKGGKPITQR
360 370 380 390 400
AGTNGYMAPE ILMEKVSYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKVSK
410 420 430 440 450
EDLKQRTLQD EVKFQHDNFT EEAKDICRLF LAKKPEQRLG SREKSDDPRK
460 470 480 490 500
HHFFKTINFP RLEAGLIEPP FVPDPSVVYA KDIAEIDDFS EVRGVEFDDK
510 520 530 540 550
DKQFFKNFAT GAVPIAWQEE IIETGLFEEL NDPNRPTGCE EGNSSKSGVC

LLL
Length:553
Mass (Da):62,212
Last modified:March 1, 2002 - v1
Checksum:iC502E301CF8EB688
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04052781R → H1 PublicationCorresponds to variant dbSNP:rs34429284Ensembl.1
Natural variantiVAR_040528113C → W1 PublicationCorresponds to variant dbSNP:rs56070798Ensembl.1
Natural variantiVAR_040529115S → C1 PublicationCorresponds to variant dbSNP:rs34769632Ensembl.1
Natural variantiVAR_040530127S → T1 PublicationCorresponds to variant dbSNP:rs35318124Ensembl.1
Natural variantiVAR_040531196V → G1 PublicationCorresponds to variant dbSNP:rs55707760Ensembl.1
Natural variantiVAR_040532196V → M1 PublicationCorresponds to variant dbSNP:rs56019094Ensembl.1
Natural variantiVAR_040533226R → W1 PublicationCorresponds to variant dbSNP:rs35566288Ensembl.1
Natural variantiVAR_040534253S → F in a metastatic melanoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_040535309E → Q1 PublicationCorresponds to variant dbSNP:rs55824414Ensembl.1
Natural variantiVAR_040536313V → I1 PublicationCorresponds to variant dbSNP:rs56076641Ensembl.1
Natural variantiVAR_040537443E → G1 PublicationCorresponds to variant dbSNP:rs36009541Ensembl.1
Natural variantiVAR_040538460P → T1 PublicationCorresponds to variant dbSNP:rs33928105Ensembl.1
Natural variantiVAR_051624461R → C. Corresponds to variant dbSNP:rs36004830Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439409 mRNA Translation: AAL48216.1
AF282269 mRNA Translation: AAL33880.1
AC112504 Genomic DNA No translation available.
CCDSiCCDS3120.1
RefSeqiNP_631948.1, NM_139209.2
XP_016861206.1, XM_017005717.1
UniGeneiHs.680654

Genome annotation databases

EnsembliENST00000264952; ENSP00000264952; ENSG00000114124
GeneIDi131890
KEGGihsa:131890
UCSCiuc011bnd.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439409 mRNA Translation: AAL48216.1
AF282269 mRNA Translation: AAL33880.1
AC112504 Genomic DNA No translation available.
CCDSiCCDS3120.1
RefSeqiNP_631948.1, NM_139209.2
XP_016861206.1, XM_017005717.1
UniGeneiHs.680654

3D structure databases

ProteinModelPortaliQ8WTQ7
SMRiQ8WTQ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126297, 2 interactors
IntActiQ8WTQ7, 1 interactor
STRINGi9606.ENSP00000264952

Chemistry databases

BindingDBiQ8WTQ7
ChEMBLiCHEMBL1075133
GuidetoPHARMACOLOGYi1471

PTM databases

iPTMnetiQ8WTQ7
PhosphoSitePlusiQ8WTQ7

Polymorphism and mutation databases

BioMutaiGRK7
DMDMi21263659

Proteomic databases

PaxDbiQ8WTQ7
PeptideAtlasiQ8WTQ7
PRIDEiQ8WTQ7
ProteomicsDBi74583

Protocols and materials databases

DNASUi131890
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264952; ENSP00000264952; ENSG00000114124
GeneIDi131890
KEGGihsa:131890
UCSCiuc011bnd.3 human

Organism-specific databases

CTDi131890
DisGeNETi131890
EuPathDBiHostDB:ENSG00000114124.2
GeneCardsiGRK7
HGNCiHGNC:17031 GRK7
MIMi606987 gene
neXtProtiNX_Q8WTQ7
OpenTargetsiENSG00000114124
PharmGKBiPA38433
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG004532
InParanoidiQ8WTQ7
KOiK00909
OMAiRTPFKDY
OrthoDBiEOG091G062G
PhylomeDBiQ8WTQ7
TreeFamiTF313940

Enzyme and pathway databases

BRENDAi2.7.11.14 2681
ReactomeiR-HSA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
SignaLinkiQ8WTQ7

Miscellaneous databases

ChiTaRSiGRK7 human
GenomeRNAii131890
PROiPR:Q8WTQ7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114124 Expressed in 20 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_GRK7
GenevisibleiQ8WTQ7 HS

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGRK7_HUMAN
AccessioniPrimary (citable) accession number: Q8WTQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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