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Protein

Stress-activated protein kinase jnk-1

Gene

jnk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which responds to activation by environmental stress by phosphorylating a number of transcription factors such as daf-16, and thus regulates transcriptional activity. By phosphorylating daf-16, plays a role in daf-16 nuclear translocation in intestinal cells in response to environmental stresses such as heat and oxidative stresses (PubMed:17894411). Downstream of jkk-1, may coordinate locomotion via type-D GABAergic motoneurons and regulates synaptic vesicle transport in conjunction with unc-16. Independently of jkk-1, may regulate some mechanosensory responses, such as response to touch (PubMed:11566876). Independently of jkk-1 and downstream of mek-1, plays a role in resistance to heavy metals, such as Cu2+ or Cd2+ (PubMed:11566876). Regulates germline cell apoptosis in response to heavy metals such as Cu2+ and arsenite (PubMed:18597494, PubMed:19497412). Required for dopaminergic CEP neuron degeneration in response to Mn2+ (PubMed:23721876). Downstream of jkk-1 but independently of mek-1, positively regulates lifespan (PubMed:15767565).7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.4 Publications

Cofactori

Mg2+4 Publications

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by either of the dual specificity kinases, jkk-1 and mek-1.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei148ATPPROSITE-ProRule annotation1
Active sitei244Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 131ATPPROSITE-ProRule annotation6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • JUN kinase activity Source: WormBase
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: WormBase
  • transcription factor binding Source: WormBase

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • hyperosmotic response Source: WormBase
  • JNK cascade Source: UniProtKB
  • JUN phosphorylation Source: WormBase
  • MAPK cascade Source: WormBase
  • negative regulation of axon regeneration Source: WormBase
  • negative regulation of cellular response to manganese ion Source: UniProtKB
  • positive regulation of neuron death Source: UniProtKB
  • protein phosphorylation Source: WormBase
  • regulation of gene expression Source: GO_Central
  • response to heat Source: WormBase
  • vesicle transport along actin filament Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 1045
ReactomeiR-CEL-193648 NRAGE signals death through JNK
R-CEL-2559580 Oxidative Stress Induced Senescence
R-CEL-2871796 FCERI mediated MAPK activation
R-CEL-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
R-CEL-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-CEL-9007892 Interleukin-38 signaling
SignaLinkiQ8WQG9

Names & Taxonomyi

Protein namesi
Recommended name:
Stress-activated protein kinase jnk-1 (EC:2.7.11.244 Publications)
Gene namesi
Name:jnk-1
ORF Names:B0478.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiB0478.1a ; CE27574 ; WBGene00002178 ; jnk-1
B0478.1b ; CE30123 ; WBGene00002178 ; jnk-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown prevents Mn2+-induced dopaminergic CEP neuron degeneration.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276T → A: Abolishes phosphorylation. Loss of kinase activity; when associated with F-278. 2 Publications1
Mutagenesisi278Y → F: Abolishes phosphorylation. Loss of kinase activity; when associated with A-276. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862701 – 463Stress-activated protein kinase jnk-1Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276Phosphothreonine3 Publications1
Modified residuei278Phosphotyrosine3 Publications1

Post-translational modificationi

Dually phosphorylated on Thr-276 and Tyr-278, which activates the enzyme.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WQG9
PaxDbiQ8WQG9
PeptideAtlasiQ8WQG9
PRIDEiQ8WQG9

PTM databases

iPTMnetiQ8WQG9

Expressioni

Tissue specificityi

Expressed in most neurons, including nerve ring, head ganglions, dorsal and ventral nerve cords and tail ganglions (PubMed:10393177). The Thr-276/Tyr-278 phosphorylated form is present in the nerve ring upon heat exposure (PubMed:17894411).2 Publications

Developmental stagei

Isoform a and isoform b are expressed in embryo and in all larval stages.1 Publication

Gene expression databases

BgeeiWBGene00002178

Interactioni

Subunit structurei

Binds to the scaffolding protein, unc-16. Unc-16 also binds other components of the JNK signaling pathway (PubMed:11738026). Interacts with daf-16 (PubMed:15767565).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
JUNP054123EBI-321822,EBI-852823From Homo sapiens.

GO - Molecular functioni

  • transcription factor binding Source: WormBase

Protein-protein interaction databases

BioGridi42580, 2 interactors
DIPiDIP-26951N
IntActiQ8WQG9, 4 interactors
STRINGi6239.B0478.1a

Structurei

3D structure databases

ProteinModelPortaliQ8WQG9
SMRiQ8WQG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 412Protein kinasePROSITE-ProRule annotationAdd BLAST294

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi276 – 278TXYCurated3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.2 Publications

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0665 Eukaryota
ENOG410XSHI LUCA
GeneTreeiENSGT00550000074271
HOGENOMiHOG000233024
InParanoidiQ8WQG9
KOiK04440
OMAiMSRHFLY
OrthoDBiEOG091G09G2
PhylomeDBiQ8WQG9

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008351 MAPK_JNK
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01772 JNKMAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q8WQG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEERLSTTSS YPSHPGRSVE EDHNTLLASS SISSIIRGTR GHLNNFIESV
60 70 80 90 100
GNWLVPSSSG RDDDAVSLDS CQSVYSPVRH HINSGTGGGI LMEPSSIHVP
110 120 130 140 150
ENYYSVTIGE AQMVVLKRYQ NLRLIGSGAQ GIVCSAFDTV RNEQVAIKKL
160 170 180 190 200
SRPFQNVTHA KRAYRELKLM SLVNHKNIIG ILNCFTPQKK LDEFNDLYIV
210 220 230 240 250
MELMDANLCQ VIQMDLDHER LSYLLYQMLC GIRHLHSAGI IHRDLKPSNI
260 270 280 290 300
VVRSDCTLKI LDFGLARTAI EAFMMTPYVV TRYYRAPEVI LGMGYKENVD
310 320 330 340 350
VWSIGCIFGE LIRGRVLFPG GDHIDQWTRI IEQLGTPDRS FLERLQPTVR
360 370 380 390 400
NYVENRPRYQ ATPFEVLFSD NMFPMTADSS RLTGAQARDL LSRMLVIDPE
410 420 430 440 450
RRISVDDALR HPYVNVWFDE IEVYAPPPLP YDHNMDVEQN VDSWREHIFR
460
ELTDYARTHD IYS
Length:463
Mass (Da):52,885
Last modified:October 1, 2002 - v2
Checksum:iE12495B45B052BC7
GO
Isoform b (identifier: Q8WQG9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.

Show »
Length:372
Mass (Da):43,179
Checksum:iD923F874F36F9856
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0502691 – 91Missing in isoform b. CuratedAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024085 mRNA Translation: BAA82640.1
FO080209 Genomic DNA Translation: CCD62004.1
FO080209 Genomic DNA Translation: CCD62005.1
PIRiT30031
T37323
RefSeqiNP_001021270.1, NM_001026099.3 [Q8WQG9-1]
NP_741434.2, NM_171371.4 [Q8WQG9-2]
UniGeneiCel.18197

Genome annotation databases

EnsemblMetazoaiB0478.1a; B0478.1a; WBGene00002178 [Q8WQG9-1]
GeneIDi177460
KEGGicel:CELE_B0478.1
UCSCiB0478.1a c. elegans [Q8WQG9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiJNK1_CAEEL
AccessioniPrimary (citable) accession number: Q8WQG9
Secondary accession number(s): Q17507, Q9UAH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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