UniProtKB - Q8W4J8 (TIF7_ARATH)
Protein TIFY 7
TIFY7
Functioni
Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY7/JAZ9 interaction (PubMed:19151223).
Interacts with and suppresses RHD6 and RSL1 transcription factor activities to negatively regulate jasmonate-stimulated root hair development (PubMed:31988260).
2 PublicationsGO - Biological processi
- defense response Source: UniProtKB-KW
- regulation of defense response Source: GO_Central
- regulation of jasmonic acid mediated signaling pathway Source: GO_Central
- response to jasmonic acid Source: TAIR
- response to wounding Source: GO_Central
Keywordsi
Biological process | Jasmonic acid signaling pathway, Plant defense, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein TIFY 7Alternative name(s): Jasmonate ZIM domain-containing protein 9 |
Gene namesi | Name:TIFY7 Synonyms:JAZ9 Ordered Locus Names:At1g70700 ORF Names:F5A18.12 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G70700 |
TAIRi | locus:2033607, AT1G70700 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 223 – 224 | RK → AA: Loss of interaction with COI1 and resistant to coronatine-mediated degradation. No effect on MYC2 binding and nuclear localization. 2 Publications | 2 | |
Mutagenesisi | 223 | R → A: No effect. | 1 | |
Mutagenesisi | 224 | K → A: No effect. | 1 | |
Mutagenesisi | 233 – 235 | KRK → AAA: Loss of interaction with MYC2 and COI1 and loss of nuclear localization. 1 Publication | 3 | |
Mutagenesisi | 233 – 235 | KRK → ARA: Sensitive to coronatine-mediated degradation and no effect on nuclear localization. 1 Publication | 3 | |
Mutagenesisi | 233 | K → A: No effect on nuclear localization. | 1 | |
Mutagenesisi | 234 | R → A: Loss of interaction with COI1 and resistant to coronatine-mediated degradation. Loss of nuclear localization. | 1 | |
Mutagenesisi | 235 | K → A: No effect on interaction with MYC2 and nuclear localization. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000300649 | 1 – 267 | Protein TIFY 7Add BLAST | 267 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q8W4J8 |
PRIDEi | Q8W4J8 |
ProteomicsDBi | 234355 [Q8W4J8-1] |
Expressioni
Inductioni
Gene expression databases
ExpressionAtlasi | Q8W4J8, baseline and differential |
Genevisiblei | Q8W4J8, AT |
Interactioni
Subunit structurei
Homo- and heterodimer.
Interacts with MYC2, MYC3, MYC4, COI1, AFPH2/NINJA, TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY5A/JAZ8, TIFY9/JAZ10 and TIFY3A/JAZ11 (PubMed:18547396, PubMed:19151223, PubMed:19309455, PubMed:20360743, PubMed:21321051, PubMed:23169619).
Interacts with RHD6 and RSL1 (PubMed:31988260).
7 PublicationsBinary interactionsi
Q8W4J8
Protein-protein interaction databases
BioGRIDi | 28627, 29 interactors |
DIPi | DIP-52213N |
ELMi | Q8W4J8 |
IntActi | Q8W4J8, 26 interactors |
STRINGi | 3702.AT1G70700.1 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8W4J8 |
SMRi | Q8W4J8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 113 – 148 | TifyPROSITE-ProRule annotationAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 220 – 244 | JasSequence analysisAdd BLAST | 25 | |
Motifi | 222 – 229 | Nuclear localization signalPROSITE-ProRule annotation | 8 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502RIU4, Eukaryota |
HOGENOMi | CLU_1257656_0_0_1 |
InParanoidi | Q8W4J8 |
OMAi | RCRDRPV |
Family and domain databases
InterProi | View protein in InterPro IPR018467, CCT_CS IPR040390, TIFY/JAZ IPR010399, Tify_dom |
PANTHERi | PTHR33077, PTHR33077, 1 hit |
Pfami | View protein in Pfam PF09425, Jas_motif, 1 hit PF06200, tify, 1 hit |
SMARTi | View protein in SMART SM00979, TIFY, 1 hit |
PROSITEi | View protein in PROSITE PS51320, TIFY, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MERDFLGLSD KQYLSNNVKH EVNDDAVEER GLSTKAAREW GKSKVFATSS
60 70 80 90 100
FMPSSDFQEA KAFPGAYQWG SVSAANVFRR CQFGGAFQNA TPLLLGGSVP
110 120 130 140 150
LPTHPSLVPR VASSGSSPQL TIFYGGTISV FNDISPDKAQ AIMLCAGNGL
160 170 180 190 200
KGETGDSKPV REAERMYGKQ IHNTAATSSS SATHTDNFSR CRDTPVAATN
210 220 230 240 250
AMSMIESFNA APRNMIPSVP QARKASLARF LEKRKERLMS AMPYKKMLLD
260
LSTGESSGMN YSSTSPT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A178WMP7 | A0A178WMP7_ARATH | TIFY domain/Divergent CCT motif fam... | TIFY7 JASMONATE-ZIM-DOMAIN PROTEIN 9, JAZ9, At1g70700, F5A18.12, F5A18_12 | 276 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 133 | D → N in AAL32593 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 133 | D → N in AAM10238 (PubMed:14593172).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027851 | 167 – 190 | Missing in isoform 2. CuratedAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC011663 Genomic DNA Translation: AAG52332.1 Sequence problems. CP002684 Genomic DNA Translation: AEE35101.1 CP002684 Genomic DNA Translation: AEE35102.1 AY062515 mRNA Translation: AAL32593.1 AY081676 mRNA Translation: AAM10238.1 AK230165 mRNA Translation: BAF01974.1 Different initiation. |
PIRi | D96731 |
RefSeqi | NP_001031264.4, NM_001036187.5 [Q8W4J8-2] NP_177227.5, NM_105738.8 [Q8W4J8-1] |
Genome annotation databases
EnsemblPlantsi | AT1G70700.1; AT1G70700.1; AT1G70700 [Q8W4J8-1] AT1G70700.2; AT1G70700.2; AT1G70700 [Q8W4J8-2] |
GeneIDi | 843407 |
Gramenei | AT1G70700.1; AT1G70700.1; AT1G70700 [Q8W4J8-1] AT1G70700.2; AT1G70700.2; AT1G70700 [Q8W4J8-2] |
KEGGi | ath:AT1G70700 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC011663 Genomic DNA Translation: AAG52332.1 Sequence problems. CP002684 Genomic DNA Translation: AEE35101.1 CP002684 Genomic DNA Translation: AEE35102.1 AY062515 mRNA Translation: AAL32593.1 AY081676 mRNA Translation: AAM10238.1 AK230165 mRNA Translation: BAF01974.1 Different initiation. |
PIRi | D96731 |
RefSeqi | NP_001031264.4, NM_001036187.5 [Q8W4J8-2] NP_177227.5, NM_105738.8 [Q8W4J8-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RS9 | X-ray | 1.95 | B | 218-239 | [»] | |
4YWC | X-ray | 2.40 | C/D | 218-239 | [»] | |
AlphaFoldDBi | Q8W4J8 | |||||
SMRi | Q8W4J8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 28627, 29 interactors |
DIPi | DIP-52213N |
ELMi | Q8W4J8 |
IntActi | Q8W4J8, 26 interactors |
STRINGi | 3702.AT1G70700.1 |
Proteomic databases
PaxDbi | Q8W4J8 |
PRIDEi | Q8W4J8 |
ProteomicsDBi | 234355 [Q8W4J8-1] |
Genome annotation databases
EnsemblPlantsi | AT1G70700.1; AT1G70700.1; AT1G70700 [Q8W4J8-1] AT1G70700.2; AT1G70700.2; AT1G70700 [Q8W4J8-2] |
GeneIDi | 843407 |
Gramenei | AT1G70700.1; AT1G70700.1; AT1G70700 [Q8W4J8-1] AT1G70700.2; AT1G70700.2; AT1G70700 [Q8W4J8-2] |
KEGGi | ath:AT1G70700 |
Organism-specific databases
Araporti | AT1G70700 |
TAIRi | locus:2033607, AT1G70700 |
Phylogenomic databases
eggNOGi | ENOG502RIU4, Eukaryota |
HOGENOMi | CLU_1257656_0_0_1 |
InParanoidi | Q8W4J8 |
OMAi | RCRDRPV |
Miscellaneous databases
PROi | PR:Q8W4J8 |
Gene expression databases
ExpressionAtlasi | Q8W4J8, baseline and differential |
Genevisiblei | Q8W4J8, AT |
Family and domain databases
InterProi | View protein in InterPro IPR018467, CCT_CS IPR040390, TIFY/JAZ IPR010399, Tify_dom |
PANTHERi | PTHR33077, PTHR33077, 1 hit |
Pfami | View protein in Pfam PF09425, Jas_motif, 1 hit PF06200, tify, 1 hit |
SMARTi | View protein in SMART SM00979, TIFY, 1 hit |
PROSITEi | View protein in PROSITE PS51320, TIFY, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TIF7_ARATH | |
Accessioni | Q8W4J8Primary (citable) accession number: Q8W4J8 Secondary accession number(s): A8MSB0 Q9CAC0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families