UniProtKB - Q8W4J2 (MPK16_ARATH)
Protein
Mitogen-activated protein kinase 16
Gene
MPK16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalytic activityi
Activity regulationi
Activated by threonine and tyrosine phosphorylation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 54 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 151 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 31 – 39 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: TAIR
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- regulation of gene expression Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 16 (EC:2.7.11.24)Short name: AtMPK16 Short name: MAP kinase 16 |
Gene namesi | Name:MPK16 Ordered Locus Names:At5g19010 ORF Names:T16G12.50 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G19010 |
TAIRi | locus:2179609, AT5G19010 |
Subcellular locationi
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- vacuole Source: TAIR
Other locations
- cytoplasm Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245816 | 1 – 567 | Mitogen-activated protein kinase 16Add BLAST | 567 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 187 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 189 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 192 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8W4J2 |
PRIDEi | Q8W4J2 |
ProteomicsDBi | 250950 |
PTM databases
iPTMneti | Q8W4J2 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q8W4J2, baseline and differential |
Genevisiblei | Q8W4J2, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 17295, 71 interactors |
IntActi | Q8W4J2, 1 interactor |
STRINGi | 3702.AT5G19010.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 316 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 292 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 187 – 189 | TXY | 3 |
Domaini
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_5_1 |
InParanoidi | Q8W4J2 |
OMAi | RSCGIPM |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q8W4J2 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8W4J2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPDHRKKSS VEVDFFTEYG EGSRYRIEEV IGKGSYGVVC SAYDTHTGEK
60 70 80 90 100
VAIKKINDIF EHVSDATRIL REIKLLRLLR HPDIVEIKHI LLPPSRREFR
110 120 130 140 150
DIYVVFELME SDLHQVIKAN DDLTPEHYQF FLYQLLRGLK YIHTANVFHR
160 170 180 190 200
DLKPKNILAN ADCKLKICDF GLARVAFNDT PTAIFWTDYV ATRWYRAPEL
210 220 230 240 250
CGSFFSKYTP AIDIWSIGCI FAELLTGKPL FPGKNVVHQL DLMTDMLGTP
260 270 280 290 300
SAEAIGRVRN EKARRYLSSM RKKKPIPFSH KFPHTDPLAL RLLEKMLSFE
310 320 330 340 350
PKDRPTAEEA LADVYFKGLA KVEREPSAQP VTKLEFEFER RRITKEDVRE
360 370 380 390 400
LIYRESLEYH PKMLKEYLDG SEPTNFMYPS AVEHFKKQFA YLEEHYKNGT
410 420 430 440 450
SHNPPERQQH ASLPRACVLY SDNNHPVAQQ SSAEVTDGLS KCSIRDERPR
460 470 480 490 500
GADRNAQMPM SRIPINVPQT IQGAAVARPG KVVGSVLRYN NCGAATGVEA
510 520 530 540 550
LEQQQRRMVR NPAAASQYPK RTQPCKSNRG DEDCATAAEG PSRLKPNTQY
560
IPQKVSAAQD TAMSRWY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 397 | K → R in AAL32607 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 397 | K → R in AAN15447 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC068809 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED92639.1 AY062529 mRNA Translation: AAL32607.1 BT000128 mRNA Translation: AAN15447.1 |
RefSeqi | NP_197402.1, NM_121906.4 |
Genome annotation databases
EnsemblPlantsi | AT5G19010.1; AT5G19010.1; AT5G19010 |
GeneIDi | 832019 |
Gramenei | AT5G19010.1; AT5G19010.1; AT5G19010 |
KEGGi | ath:AT5G19010 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC068809 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED92639.1 AY062529 mRNA Translation: AAL32607.1 BT000128 mRNA Translation: AAN15447.1 |
RefSeqi | NP_197402.1, NM_121906.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 17295, 71 interactors |
IntActi | Q8W4J2, 1 interactor |
STRINGi | 3702.AT5G19010.1 |
PTM databases
iPTMneti | Q8W4J2 |
Proteomic databases
PaxDbi | Q8W4J2 |
PRIDEi | Q8W4J2 |
ProteomicsDBi | 250950 |
Genome annotation databases
EnsemblPlantsi | AT5G19010.1; AT5G19010.1; AT5G19010 |
GeneIDi | 832019 |
Gramenei | AT5G19010.1; AT5G19010.1; AT5G19010 |
KEGGi | ath:AT5G19010 |
Organism-specific databases
Araporti | AT5G19010 |
TAIRi | locus:2179609, AT5G19010 |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_5_1 |
InParanoidi | Q8W4J2 |
OMAi | RSCGIPM |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q8W4J2 |
Miscellaneous databases
PROi | PR:Q8W4J2 |
Gene expression databases
ExpressionAtlasi | Q8W4J2, baseline and differential |
Genevisiblei | Q8W4J2, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPK16_ARATH | |
Accessioni | Q8W4J2Primary (citable) accession number: Q8W4J2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | September 5, 2006 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families