UniProtKB - Q8W2F3 (PIF4_ARATH)
Transcription factor PIF4
PIF4
Functioni
GO - Molecular functioni
- DNA binding Source: TAIR
- DNA-binding transcription factor activity Source: TAIR
- promoter-specific chromatin binding Source: TAIR
- protein dimerization activity Source: InterPro
GO - Biological processi
- de-etiolation Source: TAIR
- phototropism Source: TAIR
- red, far-red light phototransduction Source: UniProtKB-KW
- red light signaling pathway Source: TAIR
- red or far-red light signaling pathway Source: TAIR
- regulation of auxin biosynthetic process Source: TAIR
- regulation of auxin mediated signaling pathway Source: TAIR
- response to low fluence blue light stimulus by blue low-fluence system Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | Phytochrome signaling pathway, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor PIF4Alternative name(s): Basic helix-loop-helix protein 9 Short name: AtbHLH9 Short name: bHLH 9 Phytochrome-interacting factor 4 Short under red-light 2 Transcription factor EN 102 bHLH transcription factor bHLH009 |
Gene namesi | Name:PIF4 Synonyms:BHLH9, EN102, SRL2 Ordered Locus Names:At2g43010 ORF Names:MFL8.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G43010 |
TAIRi | locus:2053733, AT2G43010 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127429 | 1 – 430 | Transcription factor PIF4Add BLAST | 430 |
Proteomic databases
PaxDbi | Q8W2F3 |
ProteomicsDBi | 236753 [Q8W2F3-1] |
Expressioni
Inductioni
Gene expression databases
ExpressionAtlasi | Q8W2F3, baseline and differential |
Genevisiblei | Q8W2F3, AT |
Interactioni
Subunit structurei
Interacts preferentially with the Pfr form of phytochrome B (phyB). Binds DNA as a homodimer, but once bound to DNA, loses its capacity to interact with phyB.
Interacts with APRR1/TOC1 and PIF3. Binds to RGL2 and RGA. Forms non-functional heterodimer with HFR1.
Interacts with PHYB, CRY1 and CRY2 in the nucleus in response to low blue light (LBL) (PubMed:26724867, PubMed:12826627, PubMed:12897250, PubMed:20093430, PubMed:23224238).
Interacts with FYPP1 and FYPP3 (PubMed:31527236).
6 PublicationsBinary interactionsi
Hide detailsQ8W2F3
With | #Exp. | IntAct |
---|---|---|
BZR1 [Q8S307] | 4 | EBI-625716,EBI-1803261 |
HFR1 [Q9FE22] | 2 | EBI-625716,EBI-626001 |
RGA [Q9SLH3] | 4 | EBI-625716,EBI-963624 |
Isoform Short [Q8W2F3-2]
With | #Exp. | IntAct |
---|---|---|
PHYA [P14712] | 2 | EBI-625732,EBI-624446 |
PHYB [P14713] | 3 | EBI-625732,EBI-300727 |
PIF3 [O80536] | 3 | EBI-625732,EBI-625701 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 4240, 23 interactors |
DIPi | DIP-33903N |
IntActi | Q8W2F3, 12 interactors |
MINTi | Q8W2F3 |
STRINGi | 3702.AT2G43010.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 257 – 306 | bHLHPROSITE-ProRule annotationAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 78 – 137 | Pro-richAdd BLAST | 60 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QTIX, Eukaryota |
InParanoidi | Q8W2F3 |
PhylomeDBi | Q8W2F3 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR031066, bHLH_ALC-like_plant IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR45855, PTHR45855, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEHQGWSFEE NYSLSTNRRS IRPQDELVEL LWRDGQVVLQ SQTHREQTQT
60 70 80 90 100
QKQDHHEEAL RSSTFLEDQE TVSWIQYPPD EDPFEPDDFS SHFFSTMDPL
110 120 130 140 150
QRPTSETVKP KSSPEPPQVM VKPKACPDPP PQVMPPPKFR LTNSSSGIRE
160 170 180 190 200
TEMEQYSVTT VGPSHCGSNP SQNDLDVSMS HDRSKNIEEK LNPNASSSSG
210 220 230 240 250
GSSGCSFGKD IKEMASGRCI TTDRKRKRIN HTDESVSLSD AIGNKSNQRS
260 270 280 290 300
GSNRRSRAAE VHNLSERRRR DRINERMKAL QELIPHCSKT DKASILDEAI
310 320 330 340 350
DYLKSLQLQL QVMWMGSGMA AAAASAPMMF PGVQPQQFIR QIQSPVQLPR
360 370 380 390 400
FPVMDQSAIQ NNPGLVCQNP VQNQIISDRF ARYIGGFPHM QAATQMQPME
410 420 430
MLRFSSPAGQ QSQQPSSVPT KTTDGSRLDH
The sequence of this isoform differs from the canonical sequence as follows:
268-279: RRRDRINERMKA → VLHRFVYIIYNI
280-430: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4IQ51 | F4IQ51_ARATH | Phytochrome interacting factor 4 | PIF4 AtPIF4, MFL8.13, MFL8_13, SRL2, At2g43010 | 428 | Annotation score: | ||
A0A1P8AX08 | A0A1P8AX08_ARATH | Phytochrome interacting factor 4 | PIF4 AtPIF4, MFL8.13, MFL8_13, SRL2, At2g43010 | 472 | Annotation score: | ||
A0A178VYH3 | A0A178VYH3_ARATH | Phytochrome interacting factor 4 | PIF4 AtPIF4, MFL8.13, MFL8_13, SRL2, At2g43010 | 404 | Annotation score: | ||
A0A1P8AX01 | A0A1P8AX01_ARATH | Phytochrome interacting factor 4 | PIF4 AtPIF4, MFL8.13, MFL8_13, SRL2, At2g43010 | 437 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 204 | G → S in AAK25931 (PubMed:14593172).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 231 | H → D in strain: cv. Bla-6, cv. Et-0, cv. Li-5:3, cv. Mt-0, cv. Pa-2 and cv. Tsu-1. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002146 | 268 – 279 | RRRDR…ERMKA → VLHRFVYIIYNI in isoform Short. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_002147 | 280 – 430 | Missing in isoform Short. 1 PublicationAdd BLAST | 151 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ440755 mRNA Translation: CAD29449.1 AF251694 mRNA Translation: AAL55716.1 AC006224 Genomic DNA Translation: AAD22130.2 CP002685 Genomic DNA Translation: AEC10197.1 AY142625 mRNA Translation: AAN13083.1 AF360221 mRNA Translation: AAK25931.1 EF193514 Genomic DNA Translation: ABP96467.1 EF193515 Genomic DNA Translation: ABP96468.1 EF193516 Genomic DNA Translation: ABP96469.1 EF193517 Genomic DNA Translation: ABP96470.1 EF193518 Genomic DNA Translation: ABP96471.1 EF193519 Genomic DNA Translation: ABP96472.1 EF193520 Genomic DNA Translation: ABP96473.1 EF193521 Genomic DNA Translation: ABP96474.1 EF193522 Genomic DNA Translation: ABP96475.1 EF193523 Genomic DNA Translation: ABP96476.1 EF193524 Genomic DNA Translation: ABP96477.1 EF193525 Genomic DNA Translation: ABP96478.1 EF193526 Genomic DNA Translation: ABP96479.1 EF193527 Genomic DNA Translation: ABP96480.1 |
PIRi | H84860 |
RefSeqi | NP_565991.2, NM_129862.3 [Q8W2F3-1] |
Genome annotation databases
EnsemblPlantsi | AT2G43010.1; AT2G43010.1; AT2G43010 [Q8W2F3-1] |
GeneIDi | 818903 |
Gramenei | AT2G43010.1; AT2G43010.1; AT2G43010 [Q8W2F3-1] |
KEGGi | ath:AT2G43010 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ440755 mRNA Translation: CAD29449.1 AF251694 mRNA Translation: AAL55716.1 AC006224 Genomic DNA Translation: AAD22130.2 CP002685 Genomic DNA Translation: AEC10197.1 AY142625 mRNA Translation: AAN13083.1 AF360221 mRNA Translation: AAK25931.1 EF193514 Genomic DNA Translation: ABP96467.1 EF193515 Genomic DNA Translation: ABP96468.1 EF193516 Genomic DNA Translation: ABP96469.1 EF193517 Genomic DNA Translation: ABP96470.1 EF193518 Genomic DNA Translation: ABP96471.1 EF193519 Genomic DNA Translation: ABP96472.1 EF193520 Genomic DNA Translation: ABP96473.1 EF193521 Genomic DNA Translation: ABP96474.1 EF193522 Genomic DNA Translation: ABP96475.1 EF193523 Genomic DNA Translation: ABP96476.1 EF193524 Genomic DNA Translation: ABP96477.1 EF193525 Genomic DNA Translation: ABP96478.1 EF193526 Genomic DNA Translation: ABP96479.1 EF193527 Genomic DNA Translation: ABP96480.1 |
PIRi | H84860 |
RefSeqi | NP_565991.2, NM_129862.3 [Q8W2F3-1] |
3D structure databases
SMRi | Q8W2F3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4240, 23 interactors |
DIPi | DIP-33903N |
IntActi | Q8W2F3, 12 interactors |
MINTi | Q8W2F3 |
STRINGi | 3702.AT2G43010.1 |
Proteomic databases
PaxDbi | Q8W2F3 |
ProteomicsDBi | 236753 [Q8W2F3-1] |
Genome annotation databases
EnsemblPlantsi | AT2G43010.1; AT2G43010.1; AT2G43010 [Q8W2F3-1] |
GeneIDi | 818903 |
Gramenei | AT2G43010.1; AT2G43010.1; AT2G43010 [Q8W2F3-1] |
KEGGi | ath:AT2G43010 |
Organism-specific databases
Araporti | AT2G43010 |
TAIRi | locus:2053733, AT2G43010 |
Phylogenomic databases
eggNOGi | ENOG502QTIX, Eukaryota |
InParanoidi | Q8W2F3 |
PhylomeDBi | Q8W2F3 |
Miscellaneous databases
PROi | PR:Q8W2F3 |
Gene expression databases
ExpressionAtlasi | Q8W2F3, baseline and differential |
Genevisiblei | Q8W2F3, AT |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR031066, bHLH_ALC-like_plant IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR45855, PTHR45855, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIF4_ARATH | |
Accessioni | Q8W2F3Primary (citable) accession number: Q8W2F3 Secondary accession number(s): A5Y7D4 Q9SKX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families