Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inositol transporter 1

Gene

INT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Vacuolar inositol-proton symporter involved in the release of myo-inositol from vacuoles. Not involved in glucose or fructose transport.1 Publication

Miscellaneous

The sorting of INT1 to the tonoplast is independent of the AP-3 adapter complex but is brefeldin A sensitive.

GO - Molecular functioni

  • myo-inositol:proton symporter activity Source: TAIR

GO - Biological processi

  • myo-inositol transport Source: TAIR

Keywordsi

Biological processSymport, Transport

Enzyme and pathway databases

ReactomeiR-ATH-429593 Inositol transporters

Protein family/group databases

TCDBi2.A.1.1.66 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol transporter 1
Gene namesi
Name:INT1
Ordered Locus Names:At2g43330
ORF Names:T1O24.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43330
TAIRilocus:2058193 AT2G43330

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei39 – 59Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei74 – 94Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei109 – 129Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei132 – 152Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei159 – 179Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei192 – 212Helical; Name=6Sequence analysisAdd BLAST21
Transmembranei274 – 294Helical; Name=7Sequence analysisAdd BLAST21
Transmembranei315 – 335Helical; Name=8Sequence analysisAdd BLAST21
Transmembranei343 – 363Helical; Name=9Sequence analysisAdd BLAST21
Transmembranei374 – 394Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei423 – 443Helical; Name=11Sequence analysisAdd BLAST21
Transmembranei444 – 464Helical; Name=12Sequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Reduced root length at low concentrations of myo-inositol.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi479 – 509Missing : Leads to endoplasmic reticulum relocalization. 1 PublicationAdd BLAST31
Mutagenesisi481 – 482ER → AA: No effect on targeting. 1 Publication2
Mutagenesisi500 – 509Missing : Leads to endoplasmic reticulum relocalization. 1 Publication10
Mutagenesisi502 – 504LLE → AAA or SSS: Leads to plasma membrane relocalization. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002598751 – 509Inositol transporter 1Add BLAST509

Proteomic databases

PaxDbiQ8VZR6

Expressioni

Tissue specificityi

Expressed in seedlings, leaves, flowers and pollen grains. Not detected in seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ8VZR6 baseline and differential
GenevisibleiQ8VZR6 AT

Interactioni

Protein-protein interaction databases

BioGridi4271, 17 interactors
IntActiQ8VZR6, 17 interactors
STRINGi3702.AT2G43330.1

Structurei

3D structure databases

ProteinModelPortaliQ8VZR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi502 – 503Di-Leu2

Domaini

The C-terminal domain (465-509) is necessary and sufficient for vacuole membrane targeting. The di-Leu motif is required for this sorting.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0254 Eukaryota
ENOG410XNQK LUCA
HOGENOMiHOG000202868
InParanoidiQ8VZR6
KOiK08150
OMAiSWGVVVW
OrthoDBiEOG09360A4L
PhylomeDBiQ8VZR6

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR003663 Sugar/inositol_transpt
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF00083 Sugar_tr, 1 hit
PRINTSiPR00171 SUGRTRNSPORT
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00879 SP, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
PS00217 SUGAR_TRANSPORT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VZR6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLTIPNAPG SSGYLDMFPE RRMSYFGNSY ILGLTVTAGI GGLLFGYDTG
60 70 80 90 100
VISGALLYIK DDFEVVKQSS FLQETIVSMA LVGAMIGAAA GGWINDYYGR
110 120 130 140 150
KKATLFADVV FAAGAIVMAA APDPYVLISG RLLVGLGVGV ASVTAPVYIA
160 170 180 190 200
EASPSEVRGG LVSTNVLMIT GGQFLSYLVN SAFTQVPGTW RWMLGVSGVP
210 220 230 240 250
AVIQFILMLF MPESPRWLFM KNRKAEAIQV LARTYDISRL EDEIDHLSAA
260 270 280 290 300
EEEEKQRKRT VGYLDVFRSK ELRLAFLAGA GLQAFQQFTG INTVMYYSPT
310 320 330 340 350
IVQMAGFHSN QLALFLSLIV AAMNAAGTVV GIYFIDHCGR KKLALSSLFG
360 370 380 390 400
VIISLLILSV SFFKQSETSS DGGLYGWLAV LGLALYIVFF APGMGPVPWT
410 420 430 440 450
VNSEIYPQQY RGICGGMSAT VNWISNLIVA QTFLTIAEAA GTGMTFLILA
460 470 480 490 500
GIAVLAVIFV IVFVPETQGL TFSEVEQIWK ERAYGNISGW GSSSDSNNME

GLLEQGSQS
Length:509
Mass (Da):54,813
Last modified:March 1, 2002 - v1
Checksum:iE51EB0673ECD4E93
GO

Sequence cautioni

The sequence AAB64332 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ973175 mRNA Translation: CAJ00303.1
AC002335 Genomic DNA Translation: AAB64332.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC10252.1
AY063901 mRNA Translation: AAL36257.1
AY096505 mRNA Translation: AAM20155.1
PIRiG84864
RefSeqiNP_850393.1, NM_180062.3
UniGeneiAt.25431
At.71625

Genome annotation databases

EnsemblPlantsiAT2G43330.1; AT2G43330.1; AT2G43330
GeneIDi818934
GrameneiAT2G43330.1; AT2G43330.1; AT2G43330
KEGGiath:AT2G43330

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ973175 mRNA Translation: CAJ00303.1
AC002335 Genomic DNA Translation: AAB64332.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC10252.1
AY063901 mRNA Translation: AAL36257.1
AY096505 mRNA Translation: AAM20155.1
PIRiG84864
RefSeqiNP_850393.1, NM_180062.3
UniGeneiAt.25431
At.71625

3D structure databases

ProteinModelPortaliQ8VZR6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4271, 17 interactors
IntActiQ8VZR6, 17 interactors
STRINGi3702.AT2G43330.1

Protein family/group databases

TCDBi2.A.1.1.66 the major facilitator superfamily (mfs)

Proteomic databases

PaxDbiQ8VZR6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43330.1; AT2G43330.1; AT2G43330
GeneIDi818934
GrameneiAT2G43330.1; AT2G43330.1; AT2G43330
KEGGiath:AT2G43330

Organism-specific databases

AraportiAT2G43330
TAIRilocus:2058193 AT2G43330

Phylogenomic databases

eggNOGiKOG0254 Eukaryota
ENOG410XNQK LUCA
HOGENOMiHOG000202868
InParanoidiQ8VZR6
KOiK08150
OMAiSWGVVVW
OrthoDBiEOG09360A4L
PhylomeDBiQ8VZR6

Enzyme and pathway databases

ReactomeiR-ATH-429593 Inositol transporters

Miscellaneous databases

PROiPR:Q8VZR6

Gene expression databases

ExpressionAtlasiQ8VZR6 baseline and differential
GenevisibleiQ8VZR6 AT

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR003663 Sugar/inositol_transpt
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF00083 Sugar_tr, 1 hit
PRINTSiPR00171 SUGRTRNSPORT
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00879 SP, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
PS00217 SUGAR_TRANSPORT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiINT1_ARATH
AccessioniPrimary (citable) accession number: Q8VZR6
Secondary accession number(s): O22848
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again