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UniProtKB - Q8VYM2 (PHT11_ARATH)
Protein
Inorganic phosphate transporter 1-1
Gene
PHT1-1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
High-affinity transporter for external inorganic phosphate. Acts as a H+:phosphate symporter in both low- and high-Pi conditions. Confers sensitivity to arsenate.
3 PublicationsMiscellaneous
Although related to the sugar transporter family, it does not transport sugars.
Activity regulationi
Inhibited by protonophores (e.g. 2,4-dinitrophenol and carbonylcyanide m-chlorophenylhydrazone), the plasma membrane H+-ATPase inhibitor diethylstilbestorol, and the phosphate analog arsenate.1 Publication
Kineticsi
- KM=3 µM for inorganic phosphate1 Publication
GO - Molecular functioni
- arsenate ion transmembrane transporter activity Source: TAIR
- inorganic phosphate transmembrane transporter activity Source: TAIR
- phosphate ion transmembrane transporter activity Source: TAIR
- symporter activity Source: UniProtKB-KW
GO - Biological processi
- arsenate ion transmembrane transport Source: TAIR
- cellular response to phosphate starvation Source: TAIR
- phosphate ion transport Source: TAIR
Keywordsi
Biological process | Phosphate transport, Symport, Transport |
Protein family/group databases
TCDBi | 2.A.1.9.9, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Inorganic phosphate transporter 1-1Short name: AtPht1;1 Alternative name(s): H(+)/Pi cotransporter |
Gene namesi | Name:PHT1-1 Synonyms:APT2, PHT1, PT1 Ordered Locus Names:At5g43350 ORF Names:MWF20.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G43350 |
TAIRi | locus:2176461, AT5G43350 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 25 – 45 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 46 – 70 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 71 – 91 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 92 – 99 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 100 – 120 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 121 – 131 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 132 – 152 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 153 – 161 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 162 – 182 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 183 – 211 | ExtracellularSequence analysisAdd BLAST | 29 | |
Transmembranei | 212 – 232 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 233 – 292 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 293 – 313 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 314 – 348 | ExtracellularSequence analysisAdd BLAST | 35 | |
Transmembranei | 349 – 369 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 370 – 371 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 372 – 392 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 393 – 402 | ExtracellularSequence analysis | 10 | |
Transmembranei | 403 – 423 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 424 – 441 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 442 – 462 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 463 – 484 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 485 – 505 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 506 – 524 | CytoplasmicSequence analysisAdd BLAST | 19 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050468 | 1 – 524 | Inorganic phosphate transporter 1-1Add BLAST | 524 |
Proteomic databases
PaxDbi | Q8VYM2 |
ProteomicsDBi | 236733 |
PTM databases
iPTMneti | Q8VYM2 |
Expressioni
Tissue specificityi
Mostly expressed in roots, especially in trichoblasts and in emerging secondary roots and root hairs, but not in root tips. Also present in hydathodes, axillary buds and peripheral endosperm of germinating seeds.6 Publications
Inductioni
In roots by phosphate starvation. Repressed by auxin, cytokinins, and the Pi analog phosphite (Phi).7 Publications
Gene expression databases
ExpressionAtlasi | Q8VYM2, baseline and differential |
Genevisiblei | Q8VYM2, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 19603, 8 interactors |
IntActi | Q8VYM2, 2 interactors |
STRINGi | 3702.AT5G43350.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily. phosphate:H(+) symporter (TC 2.A.1.9) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0252, Eukaryota |
HOGENOMi | CLU_001265_46_14_1 |
InParanoidi | Q8VYM2 |
OMAi | NTMSAIG |
OrthoDBi | 762280at2759 |
PhylomeDBi | Q8VYM2 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004738, Phos_permease |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00887, 2A0109, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8VYM2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEQQLGVLK ALDVAKTQLY HFTAIVIAGM GFFTDAYDLF CVSLVTKLLG
60 70 80 90 100
RIYYFNPESA KPGSLPPHVA AAVNGVALCG TLSGQLFFGW LGDKLGRKKV
110 120 130 140 150
YGLTLVMMIL CSVASGLSFG HEAKGVMTTL CFFRFWLGFG IGGDYPLSAT
160 170 180 190 200
IMSEYANKKT RGAFIAAVFA MQGVGILAGG FVALAVSSIF DKKFPAPTYA
210 220 230 240 250
VNRALSTPPQ VDYIWRIIVM FGALPAALTY YWRMKMPETA RYTALVAKNI
260 270 280 290 300
KQATADMSKV LQTDIELEER VEDDVKDPKQ NYGLFSKEFL RRHGLHLLGT
310 320 330 340 350
TSTWFLLDIA FYSQNLFQKD IFSAIGWIPK AATMNATHEV FRIARAQTLI
360 370 380 390 400
ALCSTVPGYW FTVAFIDTIG RFKIQLNGFF MMTVFMFAIA FPYNHWIKPE
410 420 430 440 450
NRIGFVVMYS LTFFFANFGP NATTFIVPAE IFPARLRSTC HGISAAAGKA
460 470 480 490 500
GAIVGAFGFL YAAQSQDKAK VDAGYPPGIG VKNSLIMLGV LNFIGMLFTF
510 520
LVPEPKGKSL EELSGEAEVS HDEK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 185 | A → T in AAL49927 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 469 | A → P in CAA68946 (PubMed:9025304).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U62330 mRNA Translation: AAB17265.1 Y07682 Genomic DNA Translation: CAA68946.1 D86591 mRNA Translation: BAA21503.1 D86608 Genomic DNA Translation: BAA21504.1 AB025638 Genomic DNA Translation: BAA97414.1 CP002688 Genomic DNA Translation: AED94948.1 AY070432 mRNA Translation: AAL49927.1 |
RefSeqi | NP_199149.1, NM_123701.4 |
Genome annotation databases
EnsemblPlantsi | AT5G43350.1; AT5G43350.1; AT5G43350 |
GeneIDi | 834353 |
Gramenei | AT5G43350.1; AT5G43350.1; AT5G43350 |
KEGGi | ath:AT5G43350 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U62330 mRNA Translation: AAB17265.1 Y07682 Genomic DNA Translation: CAA68946.1 D86591 mRNA Translation: BAA21503.1 D86608 Genomic DNA Translation: BAA21504.1 AB025638 Genomic DNA Translation: BAA97414.1 CP002688 Genomic DNA Translation: AED94948.1 AY070432 mRNA Translation: AAL49927.1 |
RefSeqi | NP_199149.1, NM_123701.4 |
3D structure databases
SMRi | Q8VYM2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 19603, 8 interactors |
IntActi | Q8VYM2, 2 interactors |
STRINGi | 3702.AT5G43350.1 |
Protein family/group databases
TCDBi | 2.A.1.9.9, the major facilitator superfamily (mfs) |
PTM databases
iPTMneti | Q8VYM2 |
Proteomic databases
PaxDbi | Q8VYM2 |
ProteomicsDBi | 236733 |
Genome annotation databases
EnsemblPlantsi | AT5G43350.1; AT5G43350.1; AT5G43350 |
GeneIDi | 834353 |
Gramenei | AT5G43350.1; AT5G43350.1; AT5G43350 |
KEGGi | ath:AT5G43350 |
Organism-specific databases
Araporti | AT5G43350 |
TAIRi | locus:2176461, AT5G43350 |
Phylogenomic databases
eggNOGi | KOG0252, Eukaryota |
HOGENOMi | CLU_001265_46_14_1 |
InParanoidi | Q8VYM2 |
OMAi | NTMSAIG |
OrthoDBi | 762280at2759 |
PhylomeDBi | Q8VYM2 |
Miscellaneous databases
PROi | PR:Q8VYM2 |
Gene expression databases
ExpressionAtlasi | Q8VYM2, baseline and differential |
Genevisiblei | Q8VYM2, AT |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004738, Phos_permease |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00887, 2A0109, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHT11_ARATH | |
Accessioni | Q8VYM2Primary (citable) accession number: Q8VYM2 Secondary accession number(s): Q96264, Q96302 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | January 10, 2006 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families