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Protein

Geraniol 8-hydroxylase

Gene

CYP76B6

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydroxylase involved in the biosynthesis of hydroxygeraniol, a precursor of the terpenoid indole alkaloids such as vinblastine and vincristine. Also able to hydroxylate in vitro nerol and to catalyze 3'-hydroxylation of the flavanone naringenin to form eriodictyol. No activity with apigenin, kaempferol, p-coumaric acid and ferulic acid as substrates.3 Publications

Miscellaneous

The recommended numbering of geraniol gives (6E)-8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used in most publications.

Catalytic activityi

Geraniol + [reduced NADPH--hemoprotein reductase] + O2 = (6E)-8-hydroxygeraniol + [oxidized NADPH--hemoprotein reductase] + H2O.2 Publications

Cofactori

hemeBy similarity

Kineticsi

Kcat is 3.15 min (-1) for naringenin. Kcat is 7.86 min (-1) for geraniol.
  1. KM=15.81 µM for geraniol1 Publication
  2. KM=58.39 µM for naringenin1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi436Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12352
    BRENDAi1.14.13.152 1211

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Geraniol 8-hydroxylase (EC:1.14.14.832 Publications)
    Alternative name(s):
    Cytochrome P450 76B6
    Geraniol 10-hydroxylase
    Short name:
    CrG10H
    Gene namesi
    Name:CYP76B6
    Synonyms:G10H
    OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
    Taxonomic identifieri4058 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 6LumenalSequence analysis6
    Transmembranei7 – 23HelicalSequence analysisAdd BLAST17
    Topological domaini24 – 493CytoplasmicSequence analysisAdd BLAST470

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004189191 – 493Geraniol 8-hydroxylaseAdd BLAST493

    Proteomic databases

    PRIDEiQ8VWZ7

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves and flower buds. Hardly detected in mature flowers and fruits. Expressed in the internal phloem-associated parenchyma.1 Publication

    Inductioni

    Up-regulated by methyl jasmonate treatment.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VWZ7
    SMRiQ8VWZ7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK15099

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q8VWZ7-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MDYLTIILTL LFALTLYEAF SYLSRRTKNL PPGPSPLPFI GSLHLLGDQP
    60 70 80 90 100
    HKSLAKLSKK HGPIMSLKLG QITTIVISSS TMAKEVLQKQ DLAFSSRSVP
    110 120 130 140 150
    NALHAHNQFK FSVVWLPVAS RWRSLRKVLN SNIFSGNRLD ANQHLRTRKV
    160 170 180 190 200
    QELIAYCRKN SQSGEAVDVG RAAFRTSLNL LSNLIFSKDL TDPYSDSAKE
    210 220 230 240 250
    FKDLVWNIMV EAGKPNLVDF FPLLEKVDPQ GIRHRMTIHF GEVLKLFGGL
    260 270 280 290 300
    VNERLEQRRS KGEKNDVLDV LLTTSQESPE EIDRTHIERM CLDLFVAGTD
    310 320 330 340 350
    TTSSTLEWAM SEMLKNPDKM KKTQDELAQV IGRGKTIEES DINRLPYLRC
    360 370 380 390 400
    VMKETLRIHP PVPFLIPRKV EQSVEVCGYN VPKGSQVLVN AWAIGRDETV
    410 420 430 440 450
    WDDALAFKPE RFMESELDIR GRDFELIPFG AGRRICPGLP LALRTVPLML
    460 470 480 490
    GSLLNSFNWK LEGGMAPKDL DMEEKFGITL QKAHPLRAVP STL
    Length:493
    Mass (Da):55,658
    Last modified:March 1, 2002 - v1
    Checksum:i45781801A2A5A86A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ251269 mRNA Translation: CAC80883.1

    Genome annotation databases

    KEGGiag:CAC80883

    Similar proteinsi

    Entry informationi

    Entry nameiC76B6_CATRO
    AccessioniPrimary (citable) accession number: Q8VWZ7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: March 1, 2002
    Last modified: September 12, 2018
    This is version 82 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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