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Protein

Phosphothreonine lyase OspF

Gene

ospF

Organism
Shigella flexneri
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the removal of the phosphate group from the phosphothreonine in the mitogen-activated protein kinases such as MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction, thus preventing the downstream phosphorylation of histone H3. This epigenetic modification results in inhibition of the transcription of a specific subset of proinflammatory genes, and ultimately to a reduced immune response against the invading pathogen. The diminished immune response enhances the bacterium's ability to disseminate and multiply within the host.3 Publications

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by the tyrosine phosphatase inhibitor vanadate.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=204 nM for phosphorylated MAPK2 (at pH 8 and 30 degrees Celsius)2 Publications
  1. Vmax=9.89 pM/sec/µg enzyme with phosphorylated MAPK2 as substrate (at pH 8 and 30 degrees Celsius)2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei104Proton donorBy similarity1
Active sitei134Proton acceptorBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase
Biological processVirulence

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Phosphothreonine lyase OspF (EC:4.2.3.-)
Alternative name(s):
Effector protein OspF
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ospF
Synonyms:mkaD
Ordered Locus Names:CP0010
ORF Names:pWR501_0013, SFLP011
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid pWR1008 Publications
Plasmid pCP3012 Publications
Plasmid pSF51 Publication
Plasmid pINV_F6_M13821 Publication
Plasmid pSf2b_2511 Publication
Plasmid pSf1a_5711 Publication
Plasmid pSfx_5801 Publication
Plasmid pSf6_5791 Publication
Plasmid pSf3_5751 Publication
Plasmid pSf4a_5761 Publication
Plasmid pSf4b_5771 Publication
Plasmid pSf5_2461 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiShigella flexneri
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri623 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001006 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Plasmid pCP301

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi101D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi102K → R: Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. 1 Publication1
Mutagenesisi104H → A: Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. 1 Publication1
Mutagenesisi127D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi131D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi134K → A: Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. 1 Publication1
Mutagenesisi137D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi160D → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi175R → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1
Mutagenesisi218R → A: No change in Erk2 phosphothreonine lyase activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002993541 – 239Phosphothreonine lyase OspFAdd BLAST239

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q8VSP9

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Transcriptionally activated by MxiE in the intracellular environment of the host, in association with IpgC, under conditions of deregulated or active secretion. Expressed in a VirB-dependent but MxiE-independent way under conditions of non-secretion.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
RB1P064002EBI-6506625,EBI-491274From Homo sapiens.

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
Q8VSP9, 1 interactor

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I0UX-ray2.70A/B23-239[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q8VSP9

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8VSP9

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q8VSP9

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the phosphothreonine lyase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG41086SQ Bacteria
ENOG410ZMKR LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000028458

KEGG Orthology (KO)

More...
KOi
K13450

Identification of Orthologs from Complete Genome Data

More...
OMAi
FTLYVKP

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.2430.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003519 OspF/SpvC
IPR038498 OspF/SpvC_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03536 VRP3, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01342 SALVRPPROT

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8VSP9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPIKKPCLKL NLDSLNVVRS EIPQMLSANE RLKNNFNILY NQIRQYPAYY
60 70 80 90 100
FKVASNVPTY SDICQSFSVM YQGFQIVNHS GDVFIHACRE NPQSKGDFVG
110 120 130 140 150
DKFHISIARE QVPLAFQILS GLLFSEDSPI DKWKITDMNR VSQQSRVGIG
160 170 180 190 200
AQFTLYVKSD QECSQYSALL LHKIRQFIMC LESNLLRSKI APGEYPASDV
210 220 230
RPEDWKYVSY RNELRSDRDG SERQEQMLRE EPFYRLMIE
Length:239
Mass (Da):27,828
Last modified:March 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i75F6EA2D6FFD002A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti19R → K in plasmid pWR100 and plasmid pSF5. 1
Natural varianti66S → F in plasmid pWR100, plasmid pSF5, plasmid pINV_F6_M1382, plasmid pSf6_579, plasmid pSf3_575, plasmid pSf4a_576, plasmid pSf4b_577 and plasmid pSf5_246. 1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AL391753 Genomic DNA Translation: CAC05773.1
AF348706 Genomic DNA Translation: AAK18324.1
AY206429 Genomic DNA Translation: AAP78969.1
AY879342 Genomic DNA Translation: AAW64770.1
AF386526 Genomic DNA Translation: AAL72315.1
DQ362850 Genomic DNA Translation: ABD37257.1
DQ362864 Genomic DNA Translation: ABD37271.1
DQ362865 Genomic DNA Translation: ABD37272.1
DQ362866 Genomic DNA Translation: ABD37273.1
DQ362867 Genomic DNA Translation: ABD37274.1
DQ362868 Genomic DNA Translation: ABD37275.1
DQ362869 Genomic DNA Translation: ABD37276.1
DQ362870 Genomic DNA Translation: ABD37277.1

NCBI Reference Sequences

More...
RefSeqi
NP_858143.1, NC_004851.1
WP_001121867.1, NZ_UIQL01000155.1
WP_010921598.1, NZ_QWST01000360.1
YP_006960239.1, NC_019197.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAL72315; AAL72315; SF_p0010

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1238011
13917039

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sfl:CP0010

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391753 Genomic DNA Translation: CAC05773.1
AF348706 Genomic DNA Translation: AAK18324.1
AY206429 Genomic DNA Translation: AAP78969.1
AY879342 Genomic DNA Translation: AAW64770.1
AF386526 Genomic DNA Translation: AAL72315.1
DQ362850 Genomic DNA Translation: ABD37257.1
DQ362864 Genomic DNA Translation: ABD37271.1
DQ362865 Genomic DNA Translation: ABD37272.1
DQ362866 Genomic DNA Translation: ABD37273.1
DQ362867 Genomic DNA Translation: ABD37274.1
DQ362868 Genomic DNA Translation: ABD37275.1
DQ362869 Genomic DNA Translation: ABD37276.1
DQ362870 Genomic DNA Translation: ABD37277.1
RefSeqiNP_858143.1, NC_004851.1
WP_001121867.1, NZ_UIQL01000155.1
WP_010921598.1, NZ_QWST01000360.1
YP_006960239.1, NC_019197.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I0UX-ray2.70A/B23-239[»]
ProteinModelPortaliQ8VSP9
SMRiQ8VSP9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VSP9, 1 interactor

Proteomic databases

PRIDEiQ8VSP9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL72315; AAL72315; SF_p0010
GeneIDi1238011
13917039
KEGGisfl:CP0010

Phylogenomic databases

eggNOGiENOG41086SQ Bacteria
ENOG410ZMKR LUCA
HOGENOMiHOG000028458
KOiK13450
OMAiFTLYVKP

Miscellaneous databases

EvolutionaryTraceiQ8VSP9

Protein Ontology

More...
PROi
PR:Q8VSP9

Family and domain databases

Gene3Di3.30.2430.10, 1 hit
InterProiView protein in InterPro
IPR003519 OspF/SpvC
IPR038498 OspF/SpvC_sf
PfamiView protein in Pfam
PF03536 VRP3, 1 hit
PRINTSiPR01342 SALVRPPROT

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiOSPF_SHIFL
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8VSP9
Secondary accession number(s): Q2ET10
, Q2ET14, Q6XW10, Q7BEG0, Q99Q87
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2002
Last modified: January 16, 2019
This is version 88 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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