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UniProtKB - Q8VPF1 (PCAF_PSEKB)
Protein
Beta-ketoadipyl-CoA thiolase
Gene
pcaF
Organism
Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)
Status
Functioni
Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA.
Catalytic activityi
- EC:2.3.1.1741 Publication
Kineticsi
- KM=0.15 mM for 3-oxoadipyl-CoA1 Publication
- KM=0.01 mM for CoA1 Publication
pH dependencei
Optimum pH is 7.8.1 Publication
: beta-ketoadipate pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate. This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | Acyl-thioester intermediateBy similarity | 1 | |
Active sitei | 357 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 387 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 3-oxoadipyl-CoA thiolase activity Source: UniProtKB-EC
GO - Biological processi
- 3,4-dihydroxybenzoate catabolic process Source: InterPro
- beta-ketoadipate pathway Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Aromatic hydrocarbons catabolism |
Enzyme and pathway databases
SABIO-RKi | Q8VPF1 |
UniPathwayi | UPA00157;UER00263 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-ketoadipyl-CoA thiolase (EC:2.3.1.174)Alternative name(s): 3-oxoadipyl-CoA thiolase |
Gene namesi | Name:pcaF Synonyms:catF |
Organismi | Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13) |
Taxonomic identifieri | 1301098 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000337672 | 2 – 401 | Beta-ketoadipyl-CoA thiolaseAdd BLAST | 400 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
STRINGi | 1301098.PKB_2950 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0183, Bacteria |
OMAi | SMRPDDL |
OrthoDBi | 550338at2 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR012793, PcaF IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02430, pcaF, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8VPF1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSREVYICDA VRTPIGRFGG SLAAVRADDL AAVPVKALVE RNPQVDWSQL
60 70 80 90 100
DEVYLGCANQ AGEDNRNVAR MALLLAGLPD SVPGVTLNRL CASGMDAVGT
110 120 130 140 150
AFRAIASGEA ELVIAGGVES MSRAPYVMGK ADSAFGRGQK IEDTTIGWRF
160 170 180 190 200
INPLMKAQYG VDAMPETADN VADDYKVSRA DQDAFALRSQ QLAGRAQAAG
210 220 230 240 250
YFAEEIVPVV IKGKKGETVV DADEHLRPDT TLEALAKLKP VNGPDKTVTA
260 270 280 290 300
GNASGVNDGS VALILASAEA VKKHGLKARA KVLGMASAGV APRVMGIGPV
310 320 330 340 350
PAVRKLLERL NLSVADFDVI ELNEAFAAQG LAVTRELGIA DDDARVNPNG
360 370 380 390 400
GAIALGHPLG ASGARLVLTA VHQLEKSGGQ RGLCTMCVGV GQGVALAVER
V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY044272 Genomic DNA Translation: AAL02407.1 |
RefSeqi | WP_043252818.1, NZ_HG322950.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY044272 Genomic DNA Translation: AAL02407.1 |
RefSeqi | WP_043252818.1, NZ_HG322950.1 |
3D structure databases
SMRi | Q8VPF1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1301098.PKB_2950 |
Phylogenomic databases
eggNOGi | COG0183, Bacteria |
OMAi | SMRPDDL |
OrthoDBi | 550338at2 |
Enzyme and pathway databases
UniPathwayi | UPA00157;UER00263 |
SABIO-RKi | Q8VPF1 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR012793, PcaF IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02430, pcaF, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PCAF_PSEKB | |
Accessioni | Q8VPF1Primary (citable) accession number: Q8VPF1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 20, 2008 |
Last sequence update: | March 1, 2002 | |
Last modified: | September 29, 2021 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families