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UniProtKB - Q8VPF1 (PCAF_PSEKB)

Entry version 82 (02 Dec 2020)
Sequence version 1 (01 Mar 2002)
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Protein

Beta-ketoadipyl-CoA thiolase

Gene

pcaF

Organism
Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.15 mM for 3-oxoadipyl-CoA1 Publication
  2. KM=0.01 mM for CoA1 Publication

    pH dependencei

    Optimum pH is 7.8.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: beta-ketoadipate pathway

    This protein is involved in step 2 of the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. 3-oxoadipate CoA-transferase (scoA), 3-oxoadipate CoA-transferase (catI), 3-oxoadipate CoA-transferase (lpsJ), 3-oxoadipate CoA-transferase (pcaJ), 3-oxoadipate CoA-transferase subunit B (catJ), 3-oxoadipate CoA-transferase subunit A (catI), 3-oxoadipate CoA-transferase (pcaI), 3-oxoadipate CoA-transferase (PKB_4265), 3-oxoadipate CoA-transferase (catJ), 3-oxoadipate CoA-transferase (PKB_4264)
    2. 3-oxoadipyl-CoA thiolase (pcaF), Beta-ketoadipyl-CoA thiolase (pcaF), 3-oxoadipyl-CoA thiolase (paaj1)
    This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei91Acyl-thioester intermediateBy similarity1
    Active sitei357Proton acceptorPROSITE-ProRule annotation1
    Active sitei387Proton acceptorPROSITE-ProRule annotation1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    GO - Biological processi

    Complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processAromatic hydrocarbons catabolism

    Enzyme and pathway databases

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...    SABIO-RKi    		Q8VPF1

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...    UniPathwayi    		UPA00157;UER00263

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Beta-ketoadipyl-CoA thiolase (EC:2.3.1.174)
    Alternative name(s):
    3-oxoadipyl-CoA thiolase
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:pcaF
    Synonyms:catF
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1301098 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003376722 – 401Beta-ketoadipyl-CoA thiolaseAdd BLAST400

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homotetramer.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...    STRINGi    		1301098.PKB_2950

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...    SMRi    		Q8VPF1

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the thiolase-like superfamily. Thiolase family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		COG0183, Bacteria

    Identification of Orthologs from Complete Genome Data

    More...    OMAi    		IGAYEVN

    Database of Orthologous Groups

    More...    OrthoDBi    		550338at2

    Family and domain databases

    Conserved Domains Database

    More...    CDDi    		cd00751, thiolase, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...    Gene3Di    		3.40.47.10, 2 hits

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR012793, PcaF
    IPR002155, Thiolase
    IPR016039, Thiolase-like
    IPR020615, Thiolase_acyl_enz_int_AS
    IPR020610, Thiolase_AS
    IPR020617, Thiolase_C
    IPR020613, Thiolase_CS
    IPR020616, Thiolase_N

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF02803, Thiolase_C, 1 hit
    PF00108, Thiolase_N, 1 hit

    PIRSF; a whole-protein classification database

    More...    PIRSFi    		PIRSF000429, Ac-CoA_Ac_transf, 1 hit

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF53901, SSF53901, 2 hits

    TIGRFAMs; a protein family database

    More...    TIGRFAMsi    		TIGR01930, AcCoA-C-Actrans, 1 hit
    TIGR02430, pcaF, 1 hit

    PROSITE; a protein domain and family database

    More...    PROSITEi    		View protein in PROSITE
    PS00098, THIOLASE_1, 1 hit
    PS00737, THIOLASE_2, 1 hit
    PS00099, THIOLASE_3, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VPF1-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSREVYICDA VRTPIGRFGG SLAAVRADDL AAVPVKALVE RNPQVDWSQL 
            60         70         80         90        100
    DEVYLGCANQ AGEDNRNVAR MALLLAGLPD SVPGVTLNRL CASGMDAVGT 
           110        120        130        140        150
    AFRAIASGEA ELVIAGGVES MSRAPYVMGK ADSAFGRGQK IEDTTIGWRF 
           160        170        180        190        200
    INPLMKAQYG VDAMPETADN VADDYKVSRA DQDAFALRSQ QLAGRAQAAG 
           210        220        230        240        250
    YFAEEIVPVV IKGKKGETVV DADEHLRPDT TLEALAKLKP VNGPDKTVTA 
           260        270        280        290        300
    GNASGVNDGS VALILASAEA VKKHGLKARA KVLGMASAGV APRVMGIGPV 
           310        320        330        340        350
    PAVRKLLERL NLSVADFDVI ELNEAFAAQG LAVTRELGIA DDDARVNPNG 
           360        370        380        390        400
    GAIALGHPLG ASGARLVLTA VHQLEKSGGQ RGLCTMCVGV GQGVALAVER 

    V
    Length:401
    Mass (Da):41,672
    Last modified:March 1, 2002 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1A31D6FD41D25669
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		AY044272 Genomic DNA Translation: AAL02407.1

    NCBI Reference Sequences

    More...    RefSeqi    		WP_043252818.1, NZ_HG322950.1

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		AY044272 Genomic DNA Translation: AAL02407.1
    RefSeqiWP_043252818.1, NZ_HG322950.1

    3D structure databases

    SMRiQ8VPF1
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi1301098.PKB_2950

    Phylogenomic databases

    eggNOGiCOG0183, Bacteria
    OMAiIGAYEVN
    OrthoDBi550338at2

    Enzyme and pathway databases

    UniPathwayiUPA00157;UER00263
    SABIO-RKiQ8VPF1

    Family and domain databases

    CDDicd00751, thiolase, 1 hit
    Gene3Di3.40.47.10, 2 hits
    InterProiView protein in InterPro
    IPR012793, PcaF
    IPR002155, Thiolase
    IPR016039, Thiolase-like
    IPR020615, Thiolase_acyl_enz_int_AS
    IPR020610, Thiolase_AS
    IPR020617, Thiolase_C
    IPR020613, Thiolase_CS
    IPR020616, Thiolase_N
    PfamiView protein in Pfam
    PF02803, Thiolase_C, 1 hit
    PF00108, Thiolase_N, 1 hit
    PIRSFiPIRSF000429, Ac-CoA_Ac_transf, 1 hit
    SUPFAMiSSF53901, SSF53901, 2 hits
    TIGRFAMsiTIGR01930, AcCoA-C-Actrans, 1 hit
    TIGR02430, pcaF, 1 hit
    PROSITEiView protein in PROSITE
    PS00098, THIOLASE_1, 1 hit
    PS00737, THIOLASE_2, 1 hit
    PS00099, THIOLASE_3, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPCAF_PSEKB
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8VPF1
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2002
    Last modified: December 2, 2020
    This is version 82 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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