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UniProtKB - Q8VIP2 (MLXPL_RAT)
Protein
Carbohydrate-responsive element-binding protein
Gene
Mlxipl
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3' (By similarity).
By similarityGO - Molecular functioni
- carbohydrate response element binding Source: BHF-UCL
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: RGD
- protein heterodimerization activity Source: RGD
- protein kinase binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- sequence-specific DNA binding Source: RGD
GO - Biological processi
- cellular response to glucose stimulus Source: BHF-UCL
- energy homeostasis Source: UniProtKB
- fatty acid homeostasis Source: UniProtKB
- glucose homeostasis Source: RGD
- glucose mediated signaling pathway Source: RGD
- negative regulation of oxidative phosphorylation Source: RGD
- negative regulation of peptidyl-serine phosphorylation Source: RGD
- negative regulation of transcription, DNA-templated Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of fatty acid biosynthetic process Source: RGD
- positive regulation of glycolytic process Source: RGD
- positive regulation of lipid biosynthetic process Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of glycolytic process Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
- response to glucose Source: RGD
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-RNO-163358, PKA-mediated phosphorylation of key metabolic factors R-RNO-163765, ChREBP activates metabolic gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Carbohydrate-responsive element-binding proteinShort name: ChREBP Alternative name(s): Class D basic helix-loop-helix protein 14 Short name: bHLHd14 MLX interactor MLX-interacting protein-like WS basic-helix-loop-helix leucine zipper protein Short name: WS-bHLH Williams-Beuren syndrome chromosomal region 14 protein |
Gene namesi | Name:Mlxipl Synonyms:Wbscr14 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620400, Mlxipl |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Cytosol
- cytosol Source: BHF-UCL
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: RGD
- transcription regulator complex Source: RGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 568 | S → A: Impaired DNA-binding and fatty acid sensitivity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413065 | 1 – 865 | Carbohydrate-responsive element-binding proteinAdd BLAST | 865 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | PhosphoserineBy similarity | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 25 | PhosphoserineCombined sources | 1 | |
Modified residuei | 27 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 196 | PhosphoserineBy similarity | 1 | |
Modified residuei | 568 | Phosphoserine; by AMPK1 Publication | 1 | |
Modified residuei | 615 | PhosphoserineCombined sources | 1 | |
Modified residuei | 627 | PhosphoserineBy similarity | 1 | |
Modified residuei | 644 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation at Ser-568 by AMPK inactivates the DNA-binding activity.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8VIP2 |
PTM databases
iPTMneti | Q8VIP2 |
PhosphoSitePlusi | Q8VIP2 |
Interactioni
Subunit structurei
Binds DNA as a heterodimer with TCFL4/MLX.
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: RGD
- protein kinase binding Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q8VIP2 |
SMRi | Q8VIP2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 662 – 716 | bHLHPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 41 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 53 – 77 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 334 – 392 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 500 – 653 | DisorderedSequence analysisAdd BLAST | 154 | |
Regioni | 716 – 737 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 342 – 375 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 517 – 539 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 579 – 602 | Pro residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 620 – 638 | Polar residuesSequence analysisAdd BLAST | 19 |
Phylogenomic databases
InParanoidi | Q8VIP2 |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q8VIP2 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8VIP2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARALADLSV NLQVPRVVPS PDSDSDTDLE DPSPRRSAGG LHRSQVIHSG
60 70 80 90 100
HFMVSSPHSD SLTRRRDQEG PVGLADFGPR SIDPTLTRLF ECLSLAYSGK
110 120 130 140 150
LVSPKWKNFK GLKLLCRDKI RLNNAIWRAW YIQYVQRRKS PVCGFVTPLQ
160 170 180 190 200
GSEADEHRKP EAVVLEGNYW KRRIEVVMRE YHKWRIYYKK RLRKSSREGD
210 220 230 240 250
FLAPKQVEGG WPPPERWCEQ LFSSVVPVLL GGSEEEPGGR QLLDLDCFLS
260 270 280 290 300
DISDTLFTMT QPSPSSLQLP SEDAYVGNAD MIQPDLTPLQ PSLDDFMEIS
310 320 330 340 350
DFFTNYRPPQ TPTSSNFPEP PSFGPMADSL FSGGILGPEM PSPASASSSS
360 370 380 390 400
GMTPLSGNTR LQARNSCSGP LDPSTFPSSE FLLPEDPKTK MPPAPVPTPL
410 420 430 440 450
LPYPGPVKVH GLEPCTPSPF PTMAPPPALL SEEPLFSARF PFTTVPPAPG
460 470 480 490 500
VSTLPAPTTF VPTPQPGPGP GPVPFPVDHL PHGYLEPVFG PHFTVPQGVQ
510 520 530 540 550
PRCKPCSPPP GGRKASPPTL TSATASPTAT ATARDNNPCL TQLLRAAKPE
560 570 580 590 600
QVLEPSTVPS TLLRPPESPD AVPEIPRVRA FYPPIPAPTP PRPPPGPATL
610 620 630 640 650
APPRSLVVPK AERLSPPASS GSERRPSGDL NSIQPPGALS VHLSPPQTVL
660 670 680 690 700
SRGRVDNNKM ENRRITHISA EQKRRFNIKL GFDTLHGLVS TLSAQPSLKV
710 720 730 740 750
SKATTLQKTA EYILMLQQER AAMQEEAQQL RDEIEELNAA INLCQQQLPA
760 770 780 790 800
TGVPITHQRF DQMRDMFDDY VRTRTLHNWK FWVFSILIRP LFESFNGMVS
810 820 830 840 850
TASLHSLRQT SLAWLDQYCS LPALRPTVLN SLRQLSTSTS ILTDPSLVPE
860
QATRAVTEGP LGRPL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6ADP9 | A0A8I6ADP9_RAT | Carbohydrate-responsive element-bin... | Mlxipl | 866 | Annotation score: | ||
A0A8I6GKH5 | A0A8I6GKH5_RAT | Carbohydrate-responsive element-bin... | Mlxipl | 859 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB074517 mRNA Translation: BAB77523.1 |
RefSeqi | NP_598236.1, NM_133552.1 |
Genome annotation databases
GeneIDi | 171078 |
KEGGi | rno:171078 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB074517 mRNA Translation: BAB77523.1 |
RefSeqi | NP_598236.1, NM_133552.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5F74 | X-ray | 2.35 | B | 1-196 | [»] | |
AlphaFoldDBi | Q8VIP2 | |||||
SMRi | Q8VIP2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
PTM databases
iPTMneti | Q8VIP2 |
PhosphoSitePlusi | Q8VIP2 |
Proteomic databases
PaxDbi | Q8VIP2 |
Genome annotation databases
GeneIDi | 171078 |
KEGGi | rno:171078 |
Organism-specific databases
CTDi | 51085 |
RGDi | 620400, Mlxipl |
Phylogenomic databases
InParanoidi | Q8VIP2 |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q8VIP2 |
Enzyme and pathway databases
Reactomei | R-RNO-163358, PKA-mediated phosphorylation of key metabolic factors R-RNO-163765, ChREBP activates metabolic gene expression |
Miscellaneous databases
PROi | PR:Q8VIP2 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MLXPL_RAT | |
Accessioni | Q8VIP2Primary (citable) accession number: Q8VIP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references