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Protein

RWD domain-containing protein 3

Gene

Rwdd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Positively regulates the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Negatively regulates the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Has no effect on ubiquitination.1 Publication

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
RWD domain-containing protein 3
Alternative name(s):
RWD domain-containing sumoylation enhancer
Short name:
RSUME1 Publication
Gene namesi
Name:Rwdd3
Synonyms:Rsume, X2cr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1920420 Rwdd3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975461 – 267RWD domain-containing protein 3Add BLAST267

Proteomic databases

PaxDbiQ8VIL2
PRIDEiQ8VIL2

PTM databases

PhosphoSitePlusiQ8VIL2

Expressioni

Gene expression databases

CleanExiMM_RWDD3

Interactioni

Subunit structurei

Interacts with UBE2I/UBC9, NFKBIA, HIF1A and NCOA2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102075

Structurei

3D structure databases

ProteinModelPortaliQ8VIL2
SMRiQ8VIL2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 114RWDPROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15Interaction with UBE2I/UBC9By similarity3
Regioni100 – 102Interaction with UBE2I/UBC9By similarity3

Domaini

The RWD domain is required for the sumoylation enhancement activity.By similarity

Phylogenomic databases

eggNOGiENOG410IGD3 Eukaryota
ENOG4111KZ9 LUCA
HOGENOMiHOG000133077
HOVERGENiHBG057524
InParanoidiQ8VIL2
PhylomeDBiQ8VIL2

Family and domain databases

Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR006575 RWD-domain
IPR038840 RWDD3
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR15628 PTHR15628, 1 hit
PfamiView protein in Pfam
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q8VIL2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEEVRQELS ALAAIFCGPN EWEMLSCSET DGAVFRIHTT AEGLVGEDVP
60 70 80 90 100
LELAFHLPVG YPLCLPGISV TSEHLTRAQC VTAKEKLLGE ARKLVSEPMV
110 120 130 140 150
HELVLWIQQN LRLVLSQPET VSSHEKCTLP ESATGDDGPW MTLLRLDHMR
160 170 180 190 200
ARTKYVKAVE KWASELRLTG RLMFMGKLIL ILLQGDRSNI KEYLILQKTS
210 220 230 240 250
KVDVDSSGKK CKEKMISVLS ETKVQTEHKR FLAFEVKEYS TLEELQKEFG
260
AAGLGELFSE CVLGLVK
Length:267
Mass (Da):29,890
Last modified:March 1, 2002 - v1
Checksum:iE600189E342EE8F4
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q1R6E9Q1R6_MOUSE
RWD domain-containing protein 3
Rwdd3
339Annotation score:
E9PZT2E9PZT2_MOUSE
RWD domain-containing protein 3
Rwdd3
200Annotation score:
Q3UYX7Q3UYX7_MOUSE
MCG4676, isoform CRA_a
Rwdd3 mCG_4676
267Annotation score:
E9PVR5E9PVR5_MOUSE
RWD domain-containing protein 3
Rwdd3
140Annotation score:
F7AYC4F7AYC4_MOUSE
RWD domain-containing protein 3
Rwdd3
97Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121V → A in BAB29171 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439556 mRNA Translation: AAL35585.1
AK014130 mRNA Translation: BAB29171.2
CCDSiCCDS17798.1
UniGeneiMm.263163

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439556 mRNA Translation: AAL35585.1
AK014130 mRNA Translation: BAB29171.2
CCDSiCCDS17798.1
UniGeneiMm.263163

3D structure databases

ProteinModelPortaliQ8VIL2
SMRiQ8VIL2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102075

PTM databases

PhosphoSitePlusiQ8VIL2

Proteomic databases

PaxDbiQ8VIL2
PRIDEiQ8VIL2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1920420 Rwdd3

Phylogenomic databases

eggNOGiENOG410IGD3 Eukaryota
ENOG4111KZ9 LUCA
HOGENOMiHOG000133077
HOVERGENiHBG057524
InParanoidiQ8VIL2
PhylomeDBiQ8VIL2

Miscellaneous databases

PROiPR:Q8VIL2
SOURCEiSearch...

Gene expression databases

CleanExiMM_RWDD3

Family and domain databases

Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR006575 RWD-domain
IPR038840 RWDD3
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR15628 PTHR15628, 1 hit
PfamiView protein in Pfam
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRWDD3_MOUSE
AccessioniPrimary (citable) accession number: Q8VIL2
Secondary accession number(s): Q9CXQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2002
Last modified: June 20, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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