UniProtKB - Q8VIJ6 (SFPQ_MOUSE)
Splicing factor, proline- and glutamine-rich
Sfpq
Functioni
GO - Molecular functioni
- chromatin binding Source: BHF-UCL
- DNA binding Source: MGI
- E-box binding Source: UniProtKB
- histone deacetylase binding Source: MGI
- nucleic acid binding Source: GO_Central
- protein homodimerization activity Source: MGI
- RNA binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- activation of innate immune response Source: MGI
- alternative mRNA splicing, via spliceosome Source: MGI
- cellular response to DNA damage stimulus Source: MGI
- chromosome organization Source: MGI
- double-strand break repair via homologous recombination Source: MGI
- histone H3 deacetylation Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- mRNA splicing, via spliceosome Source: GO_Central
- negative regulation of circadian rhythm Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: MGI
- positive regulation of sister chromatid cohesion Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of cell cycle Source: MGI
- regulation of circadian rhythm Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor, RNA-binding |
Biological process | Biological rhythms, DNA damage, DNA repair, Immunity, Innate immunity, mRNA processing, mRNA splicing, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-8849468, PTK6 Regulates Proteins Involved in RNA Processing |
Names & Taxonomyi
Protein namesi | Recommended name: Splicing factor, proline- and glutamine-richAlternative name(s): DNA-binding p52/p100 complex, 100 kDa subunit Polypyrimidine tract-binding protein-associated-splicing factor Short name: PSF Short name: PTB-associated-splicing factor |
Gene namesi | Name:Sfpq Synonyms:Psf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918764, Sfpq |
Subcellular locationi
Nucleus
- Nucleus speckle By similarity
- Nucleus matrix 1 Publication
Other locations
- Cytoplasm By similarity
Note: Predominantly in nuclear matrix.By similarity
Nucleus
- nuclear matrix Source: MGI
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: MGI
- paraspeckles Source: MGI
Other locations
- chromatin Source: MGI
- cytoplasm Source: UniProtKB-SubCell
- RNA polymerase II transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081910 | 1 – 699 | Splicing factor, proline- and glutamine-richAdd BLAST | 699 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | Phosphoserine; by MKNK2By similarity | 1 | |
Modified residuei | 9 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 9 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 33 | PhosphoserineBy similarity | 1 | |
Modified residuei | 200 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 228 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 234 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 237 | Omega-N-methylarginineCombined sources | 1 | |
Cross-linki | 263 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 265 | PhosphoserineBy similarity | 1 | |
Cross-linki | 271 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 275 | Phosphoserine; by MKNK2By similarity | 1 | |
Modified residuei | 285 | Phosphotyrosine; by ALKBy similarity | 1 | |
Modified residuei | 306 | N6,N6-dimethyllysineBy similarity | 1 | |
Modified residuei | 311 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 330 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 330 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 360 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 366 | PhosphoserineBy similarity | 1 | |
Modified residuei | 371 | PhosphoserineBy similarity | 1 | |
Modified residuei | 413 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 464 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 488 | PhosphoserineBy similarity | 1 | |
Modified residuei | 563 | Dimethylated arginineBy similarity | 1 | |
Modified residuei | 618 | PhosphoserineBy similarity | 1 | |
Modified residuei | 673 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 679 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 683 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 685 | Dimethylated arginine; alternateBy similarity | 1 | |
Modified residuei | 685 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 687 | Omega-N-methylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8VIJ6 |
jPOSTi | Q8VIJ6 |
MaxQBi | Q8VIJ6 |
PaxDbi | Q8VIJ6 |
PeptideAtlasi | Q8VIJ6 |
PRIDEi | Q8VIJ6 |
2D gel databases
REPRODUCTION-2DPAGEi | Q8VIJ6 |
PTM databases
iPTMneti | Q8VIJ6 |
PhosphoSitePlusi | Q8VIJ6 |
SwissPalmi | Q8VIJ6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028820, Expressed in forelimb bud and 315 other tissues |
Genevisiblei | Q8VIJ6, MM |
Interactioni
Subunit structurei
Heterodimer with NONO. Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. The coiled coil domain mediates interaction with NONO, and can also mediate formation of long, linear homooligomers (in vitro). SFPQ is a component of spliceosome and U5.4/6 snRNP complexes.
Interacts with SNRPA/U1A.
Component of a snRNP-free complex with SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3.
Interacts with polypyrimidine tract-binding protein 1/PTB.
Part of a complex consisting of SFPQ, NONO and NR5A1.
Interacts with RXRA, probably THRA, and SIN3A.
Interacts with TOP1.
Part of a complex consisting of SFPQ, NONO and TOP1.
Interacts with SNRNP70 in apoptotic cells.
Interacts with PSPC1 (PubMed:15140795).
Interacts with RNF43 (By similarity).
Interacts with PITX3 and NR4A2/NURR1 (PubMed:19144721).
Interacts with PTK6.
Interacts with THRAP3; the interaction is dependent on SFPQ phosphorylation at 'Thr-687' and inhibits binding of SFPQ to a ESS1 exonic splicing silencer element-containing RNA (By similarity). The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A (PubMed:21680841).
Interacts with PER1 and PER2 (PubMed:22966205). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA (By similarity).
Interacts with PQBP1.
Component of a multiprotein complex with NONO and WASL (By similarity).
Interacts with ERCC6 (By similarity).
By similarity4 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- histone deacetylase binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 214752, 62 interactors |
IntActi | Q8VIJ6, 12 interactors |
MINTi | Q8VIJ6 |
STRINGi | 10090.ENSMUSP00000030623 |
Miscellaneous databases
RNActi | Q8VIJ6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 9 – 11 | 1 | 3 | |
Repeati | 19 – 21 | 2 | 3 | |
Repeati | 25 – 27 | 3 | 3 | |
Domaini | 289 – 361 | RRM 1PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 363 – 444 | RRM 2PROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 27 | 3 X 3 AA repeats of R-G-GAdd BLAST | 19 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 489 – 588 | Sequence analysisAdd BLAST | 100 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 258 | Gln/Glu/Pro-richAdd BLAST | 249 | |
Compositional biasi | 10 – 15 | Poly-Gly | 6 | |
Compositional biasi | 20 – 27 | Poly-Gly | 8 | |
Compositional biasi | 54 – 63 | Poly-Pro | 10 | |
Compositional biasi | 65 – 69 | Poly-Gln | 5 | |
Compositional biasi | 96 – 100 | Poly-Pro | 5 | |
Compositional biasi | 158 – 161 | Poly-Pro | 4 | |
Compositional biasi | 178 – 182 | Poly-Pro | 5 | |
Compositional biasi | 563 – 566 | Poly-Arg | 4 | |
Compositional biasi | 605 – 608 | Poly-Gly | 4 | |
Compositional biasi | 627 – 633 | Poly-Gly | 7 |
Domaini
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0115, Eukaryota |
GeneTreei | ENSGT00940000156221 |
HOGENOMi | CLU_027185_1_0_1 |
InParanoidi | Q8VIJ6 |
OMAi | DMRAERF |
OrthoDBi | 1274880at2759 |
PhylomeDBi | Q8VIJ6 |
TreeFami | TF315795 |
Family and domain databases
CDDi | cd12948, NOPS_PSF, 1 hit cd12587, RRM1_PSF, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012975, NOPS IPR012677, Nucleotide-bd_a/b_plait_sf IPR034526, PSF_NOPS IPR034525, PSF_RRM1 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF08075, NOPS, 1 hit PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSRDRFRSRG GGGGGFHRRG GGGGRGGLHD FRSPPPGMGL NQNRGPMGPG
60 70 80 90 100
PGGPKPPLPP PPPHQQQQQP PPQQPPPQQP PPHQQPPPHQ PPHQQPPPPP
110 120 130 140 150
QESKPVVPQG PGSAPGVSSA PPPAVSAPPA NPPTTGAPPG PGPTPTPPPA
160 170 180 190 200
VPSTAPGPPP PSTPSSGVST TPPQTGGPPP PPAGGAGPGP KPGPGPGGPK
210 220 230 240 250
GGKMPGGPKP GGGPGMGAPG GHPKPPHRGG GEPRGGRQHH APYHQQHHQG
260 270 280 290 300
PPPGGPGPRT EEKISDSEGF KANLSLLRRP GEKTYTQRCR LFVGNLPADI
310 320 330 340 350
TEDEFKRLFA KYGEPGEVFI NKGKGFGFIK LESRALAEIA KAELDDTPMR
360 370 380 390 400
GRQLRVRFAT HAAALSVRNL SPYVSNELLE EAFSQFGPIE RAVVIVDDRG
410 420 430 440 450
RSTGKGIVEF ASKPAARKAF ERCSEGVFLL TTTPRPVIVE PLEQLDDEDG
460 470 480 490 500
LPEKLAQKNP MYQKERETPP RFAQHGTFEY EYSQRWKSLD EMEKQQREQV
510 520 530 540 550
EKNMKDAKDK LESEMEDAYH EHQANLLRQD LMRRQEELRR MEELHSQEMQ
560 570 580 590 600
KRKEMQLRQE EERRRREEEM MIRQREMEEQ MRRQREESYS RMGYMDPRER
610 620 630 640 650
DMRMGGGGTM NMGDPYGSGG QKFPPLGGGG GIGYEANPGV PPATMSGSMM
660 670 680 690
GSDMRTERFG QGGAGPVGGQ GPRGMGPGTP AGYGRGREEY EGPNKKPRF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | M → Q AA sequence (PubMed:11008015).Curated | 1 | |
Sequence conflicti | 546 | S → N in AAG17365 (PubMed:11008015).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY034062 mRNA Translation: AAK60397.1 AL606985 Genomic DNA No translation available. BC089305 mRNA Translation: AAH89305.1 AF272847 mRNA Translation: AAG17365.1 |
CCDSi | CCDS18662.1 |
RefSeqi | NP_076092.1, NM_023603.3 |
Genome annotation databases
Ensembli | ENSMUST00000030623; ENSMUSP00000030623; ENSMUSG00000028820 |
GeneIDi | 71514 |
KEGGi | mmu:71514 |
UCSCi | uc008utz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY034062 mRNA Translation: AAK60397.1 AL606985 Genomic DNA No translation available. BC089305 mRNA Translation: AAH89305.1 AF272847 mRNA Translation: AAG17365.1 |
CCDSi | CCDS18662.1 |
RefSeqi | NP_076092.1, NM_023603.3 |
3D structure databases
SMRi | Q8VIJ6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214752, 62 interactors |
IntActi | Q8VIJ6, 12 interactors |
MINTi | Q8VIJ6 |
STRINGi | 10090.ENSMUSP00000030623 |
PTM databases
iPTMneti | Q8VIJ6 |
PhosphoSitePlusi | Q8VIJ6 |
SwissPalmi | Q8VIJ6 |
2D gel databases
REPRODUCTION-2DPAGEi | Q8VIJ6 |
Proteomic databases
EPDi | Q8VIJ6 |
jPOSTi | Q8VIJ6 |
MaxQBi | Q8VIJ6 |
PaxDbi | Q8VIJ6 |
PeptideAtlasi | Q8VIJ6 |
PRIDEi | Q8VIJ6 |
Protocols and materials databases
Antibodypediai | 4083, 299 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030623; ENSMUSP00000030623; ENSMUSG00000028820 |
GeneIDi | 71514 |
KEGGi | mmu:71514 |
UCSCi | uc008utz.2, mouse |
Organism-specific databases
CTDi | 6421 |
MGIi | MGI:1918764, Sfpq |
Phylogenomic databases
eggNOGi | KOG0115, Eukaryota |
GeneTreei | ENSGT00940000156221 |
HOGENOMi | CLU_027185_1_0_1 |
InParanoidi | Q8VIJ6 |
OMAi | DMRAERF |
OrthoDBi | 1274880at2759 |
PhylomeDBi | Q8VIJ6 |
TreeFami | TF315795 |
Enzyme and pathway databases
Reactomei | R-MMU-8849468, PTK6 Regulates Proteins Involved in RNA Processing |
Miscellaneous databases
BioGRID-ORCSi | 71514, 8 hits in 19 CRISPR screens |
ChiTaRSi | Sfpq, mouse |
PROi | PR:Q8VIJ6 |
RNActi | Q8VIJ6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028820, Expressed in forelimb bud and 315 other tissues |
Genevisiblei | Q8VIJ6, MM |
Family and domain databases
CDDi | cd12948, NOPS_PSF, 1 hit cd12587, RRM1_PSF, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012975, NOPS IPR012677, Nucleotide-bd_a/b_plait_sf IPR034526, PSF_NOPS IPR034525, PSF_RRM1 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF08075, NOPS, 1 hit PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SFPQ_MOUSE | |
Accessioni | Q8VIJ6Primary (citable) accession number: Q8VIJ6 Secondary accession number(s): A2A7U6, Q9ERW2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot