We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - Q8VID6 (PDE11_RAT)
Protein
Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
Gene
Pde11a
Organism
Rattus norvegicus (Rat)
Status
Functioni
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP (PubMed:11502204).
Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively (PubMed:11502204).
1 PublicationCatalytic activityi
Cofactori
a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity
Activity regulationi
Inhibited by 3-isobutyl-1-methylxanthine (IBMX), zaprinast and dipyridamole. cGMP acts as an allosteric activator.1 Publication
Kineticsi
- KM=3.9 µM for cAMP (isoform 1)1 Publication
- KM=1.6 µM for cGMP (isoform 1)1 Publication
- KM=4.0 µM for cAMP (isoform 2)1 Publication
- KM=1.6 µM for cGMP (isoform 2)1 Publication
- KM=2.2 µM for cAMP (isoform 3)1 Publication
- KM=1.3 µM for cGMP (isoform 3)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 424 | 3',5'-cGMPBy similarity | 1 | |
Active sitei | 664 | Proton donorBy similarity | 1 | |
Metal bindingi | 668 | Divalent metal cation 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 704 | Divalent metal cation 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 705 | Divalent metal cation 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 705 | Divalent metal cation 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 816 | Divalent metal cation 1PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB-EC
- 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
- 3',5'-cyclic-nucleotide phosphodiesterase activity Source: GO_Central
- cGMP binding Source: RGD
- cGMP-stimulated cyclic-nucleotide phosphodiesterase activity Source: UniProtKB
- cyclic-nucleotide phosphodiesterase activity Source: RGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- metabolic process Source: UniProtKB-KW
- negative regulation of cAMP-mediated signaling Source: RGD
- negative regulation of cGMP-mediated signaling Source: RGD
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase |
Ligand | cAMP, cGMP, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-418457, cGMP effects R-RNO-418555, G alpha (s) signalling events |
SABIO-RKi | Q8VID6 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621793, Pde11a |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Other locations
- perikaryon Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247042 | 1 – 935 | Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11AAdd BLAST | 935 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 162 | PhosphoserineBy similarity | 1 | |
Modified residuei | 163 | PhosphoserineBy similarity | 1 | |
Modified residuei | 239 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8VID6 |
PRIDEi | Q8VID6 |
PTM databases
iPTMneti | Q8VID6 |
PhosphoSitePlusi | Q8VID6 |
Expressioni
Tissue specificityi
Isoform 1 is expressed in brain, heart, kidney and liver, but not in prostate. Isoform 2 is specifically expressed in testis. Isoform 3 is expressed in various tissues including brain, lung, skeletal muscle, spleen, testis and prostate.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000024457, Expressed in testis and 19 other tissues |
ExpressionAtlasi | Q8VID6, baseline and differential |
Genevisiblei | Q8VID6, RN |
Interactioni
Protein-protein interaction databases
BioGRIDi | 250862, 1 interactor |
STRINGi | 10116.ENSRNOP00000008300 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 217 – 370 | GAF 1Add BLAST | 154 | |
Domaini | 402 – 558 | GAF 2Add BLAST | 157 | |
Domaini | 588 – 912 | PDEasePROSITE-ProRule annotationAdd BLAST | 325 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 41 – 125 | DisorderedSequence analysisAdd BLAST | 85 | |
Regioni | 915 – 935 | DisorderedSequence analysisAdd BLAST | 21 |
Domaini
The tandem GAF domains bind cGMP, and regulate enzyme activity. The binding of cGMP stimulates enzyme activity.By similarity
Sequence similaritiesi
Belongs to the cyclic nucleotide phosphodiesterase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3689, Eukaryota |
GeneTreei | ENSGT00940000162151 |
HOGENOMi | CLU_006980_0_1_1 |
InParanoidi | Q8VID6 |
OMAi | KGECDWN |
OrthoDBi | 904682at2759 |
PhylomeDBi | Q8VID6 |
TreeFami | TF316499 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit 3.30.450.40, 2 hits |
InterProi | View protein in InterPro IPR003018, GAF IPR029016, GAF-like_dom_sf IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF01590, GAF, 2 hits PF00233, PDEase_I, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00065, GAF, 2 hits SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8VID6-1) [UniParc]FASTAAdd to basket
Also known as: PDE11A41 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASRLDFGE VETFLDRHPE LFEDYLMRKG KQELVDKWLQ RHSSGQGASD
60 70 80 90 100
LRPALAGASS LAQSSARGST GIGGGAGPQG SANSHPASGG GESAGVPLSP
110 120 130 140 150
SWASGSRGDG NLQRRASQKE LRKSFARSKA IHVNRTYDEQ VTSRAQEPLS
160 170 180 190 200
SVRRRALLRK ASSLPPTTAH ILSALLESRV NLPQYPPTAI DYKCHLKKHN
210 220 230 240 250
ERQFFLELVK DISNDLDLTS LSYKILIFVC LMVDADRCSL FLVEGAAAGK
260 270 280 290 300
KTLVSKFFDV HAGTPLLPCS TTENSNEVQV PWGKGIIGYV GEHGETVNIP
310 320 330 340 350
DAYQDRRFND EIDKLTGYKT KSLLCMPIRN SDGEIIGVAQ AINKVPEGAP
360 370 380 390 400
FTEDDEKVMQ MYLPFCGIAI SNAQLFAASR KEYERSRALL EVVNDLFEEQ
410 420 430 440 450
TDLEKIVKKI MHRAQTLLKC ERCSVLLLED IESPVVKFTK SFELMSPKCS
460 470 480 490 500
ADAENSFKES VEKSSYSDWL INNSIAELVA STGLPVNVSD AYQDPRFDAE
510 520 530 540 550
ADQISGFHIR SVLCVPIWNS NHQIIGVAQV LNRLDGKPFD DADQRLFEAF
560 570 580 590 600
VIFCGLGINN TIMYDQVKKS WAKQSVALDV LSYHATCSKA EVDKFKAANI
610 620 630 640 650
PLVSELAIDD IHFDDFSLDV DAMITAALRM FMELGMVQKF KIDYETLCRW
660 670 680 690 700
LLTVRKNYRM VLYHNWRHAF NVCQLMFAML TTAGFQEILT EVEILAVIVG
710 720 730 740 750
CLCHDLDHRG TNNAFQAKSD SALAQLYGTS ATLEHHHFNH AVMILQSEGH
760 770 780 790 800
NIFANLSSKE YSDLMQLLKQ SILATDLTLY FERRTEFFEL VSKGAYDWSI
810 820 830 840 850
TSHRDVFRSM LMTACDLGAV TKPWEISRQV AELVTSEFFE QGDRERSELK
860 870 880 890 900
LTPSAIFDRN RKDELPRLQL EWIDSICMPL YQALVKVNAK LKPMLDSVAA
910 920 930
NRRKWEELHQ KRLQVSAASP VPSSPSPAVA GEDRL
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019902 | 1 – 354 | Missing in isoform 3. 1 PublicationAdd BLAST | 354 | |
Alternative sequenceiVSP_019903 | 1 – 250 | Missing in isoform 2. 1 PublicationAdd BLAST | 250 | |
Alternative sequenceiVSP_019904 | 251 – 304 | KTLVS…PDAYQ → MLKQARRFSFRNVRSATQWR KVGSTRQGQISGAFLAERLD KHQDFLTRMQTRTK in isoform 2. 1 PublicationAdd BLAST | 54 | |
Alternative sequenceiVSP_019905 | 355 – 357 | DEK → MSW in isoform 3. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB059360 mRNA Translation: BAB79627.1 AB059361 mRNA Translation: BAB79628.1 AB059362 mRNA Translation: BAB79629.1 |
RefSeqi | NP_001120952.1, NM_001127480.1 [Q8VID6-2] NP_001120953.1, NM_001127481.2 [Q8VID6-3] NP_543169.1, NM_080893.1 [Q8VID6-1] |
Genome annotation databases
Ensembli | ENSRNOT00000045862; ENSRNOP00000048657; ENSRNOG00000024457 [Q8VID6-3] ENSRNOT00000050355; ENSRNOP00000051289; ENSRNOG00000024457 [Q8VID6-2] |
GeneIDi | 140928 |
KEGGi | rno:140928 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB059360 mRNA Translation: BAB79627.1 AB059361 mRNA Translation: BAB79628.1 AB059362 mRNA Translation: BAB79629.1 |
RefSeqi | NP_001120952.1, NM_001127480.1 [Q8VID6-2] NP_001120953.1, NM_001127481.2 [Q8VID6-3] NP_543169.1, NM_080893.1 [Q8VID6-1] |
3D structure databases
AlphaFoldDBi | Q8VID6 |
SMRi | Q8VID6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250862, 1 interactor |
STRINGi | 10116.ENSRNOP00000008300 |
PTM databases
iPTMneti | Q8VID6 |
PhosphoSitePlusi | Q8VID6 |
Proteomic databases
PaxDbi | Q8VID6 |
PRIDEi | Q8VID6 |
Genome annotation databases
Ensembli | ENSRNOT00000045862; ENSRNOP00000048657; ENSRNOG00000024457 [Q8VID6-3] ENSRNOT00000050355; ENSRNOP00000051289; ENSRNOG00000024457 [Q8VID6-2] |
GeneIDi | 140928 |
KEGGi | rno:140928 |
Organism-specific databases
CTDi | 50940 |
RGDi | 621793, Pde11a |
Phylogenomic databases
eggNOGi | KOG3689, Eukaryota |
GeneTreei | ENSGT00940000162151 |
HOGENOMi | CLU_006980_0_1_1 |
InParanoidi | Q8VID6 |
OMAi | KGECDWN |
OrthoDBi | 904682at2759 |
PhylomeDBi | Q8VID6 |
TreeFami | TF316499 |
Enzyme and pathway databases
Reactomei | R-RNO-418457, cGMP effects R-RNO-418555, G alpha (s) signalling events |
SABIO-RKi | Q8VID6 |
Miscellaneous databases
PROi | PR:Q8VID6 |
Gene expression databases
Bgeei | ENSRNOG00000024457, Expressed in testis and 19 other tissues |
ExpressionAtlasi | Q8VID6, baseline and differential |
Genevisiblei | Q8VID6, RN |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit 3.30.450.40, 2 hits |
InterProi | View protein in InterPro IPR003018, GAF IPR029016, GAF-like_dom_sf IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF01590, GAF, 2 hits PF00233, PDEase_I, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00065, GAF, 2 hits SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDE11_RAT | |
Accessioni | Q8VID6Primary (citable) accession number: Q8VID6 Secondary accession number(s): Q8VID7, Q8VID8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families