UniProtKB - Q8VI93 (OAS3_MOUSE)
Protein
2'-5'-oligoadenylate synthase 3
Gene
Oas3
Organism
Mus musculus (Mouse)
Status
Functioni
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.1 Publication
Catalytic activityi
- EC:2.7.7.841 Publication
Cofactori
Mg2+Curated
Activity regulationi
Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. Strongly activated by long dsRNAs at least 50 nucleotides in length. ssRNA does not activate the enzyme.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 855 | ATPBy similarity | 1 | |
Metal bindingi | 867 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 869 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 939 | Magnesium; catalyticBy similarity | 1 | |
Binding sitei | 998 | ATPBy similarity | 1 | |
Binding sitei | 1001 | ATPBy similarity | 1 | |
Binding sitei | 1020 | ATPBy similarity | 1 |
GO - Molecular functioni
- 2'-5'-oligoadenylate synthetase activity Source: MGI
- ATP binding Source: UniProtKB
- double-stranded RNA binding Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: MGI
- innate immune response Source: UniProtKB-KW
- negative regulation of viral genome replication Source: MGI
- regulation of ribonuclease activity Source: MGI
- response to virus Source: UniProtKB
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase |
Biological process | Antiviral defense, Immunity, Innate immunity |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 2'-5'-oligoadenylate synthase 3 (EC:2.7.7.841 Publication)Short name: (2-5')oligo(A) synthase 3 Short name: 2-5A synthase 3 Alternative name(s): 2',5'-oligoadenylate synthetase-like 10 |
Gene namesi | Name:Oas3 Synonyms:oasl10 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180850, Oas3 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: MGI
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418630 | 1 – 1138 | 2'-5'-oligoadenylate synthase 3Add BLAST | 1138 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q8VI93 |
jPOSTi | Q8VI93 |
MaxQBi | Q8VI93 |
PaxDbi | Q8VI93 |
PeptideAtlasi | Q8VI93 |
PRIDEi | Q8VI93 |
PTM databases
iPTMneti | Q8VI93 |
PhosphoSitePlusi | Q8VI93 |
SwissPalmi | Q8VI93 |
Expressioni
Tissue specificityi
Intestine.1 Publication
Inductioni
By type I interferon (IFN) and viruses.
Gene expression databases
Bgeei | ENSMUSG00000032661, Expressed in granulocyte and 72 other tissues |
Genevisiblei | Q8VI93, MM |
Interactioni
Subunit structurei
Monomer.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 154 | Interaction with dsRNABy similarity | 1 | |
Sitei | 242 | Interaction with dsRNABy similarity | 1 |
Protein-protein interaction databases
BioGRIDi | 232935, 6 interactors |
STRINGi | 10090.ENSMUSP00000035588 |
Miscellaneous databases
RNActi | Q8VI93, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 6 – 341 | OAS domain 1CuratedAdd BLAST | 336 | |
Regioni | 12 – 56 | Interaction with dsRNABy similarityAdd BLAST | 45 | |
Regioni | 185 – 199 | Interaction with dsRNABy similarityAdd BLAST | 15 | |
Regioni | 342 – 462 | LinkerCuratedAdd BLAST | 121 | |
Regioni | 463 – 793 | OAS domain 2CuratedAdd BLAST | 331 | |
Regioni | 801 – 1135 | OAS domain 3CuratedAdd BLAST | 335 |
Domaini
OAS domain 3 is catalytically active. OAS domain 1 has no catalytic activity but is essential for recognition of long dsRNAs.By similarity
Sequence similaritiesi
Belongs to the 2-5A synthase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502S649, Eukaryota |
GeneTreei | ENSGT00510000046406 |
HOGENOMi | CLU_287245_0_0_1 |
InParanoidi | Q8VI93 |
OMAi | CNKMPKG |
OrthoDBi | 611234at2759 |
PhylomeDBi | Q8VI93 |
TreeFami | TF329749 |
Family and domain databases
CDDi | cd05400, NT_2-5OAS_ClassI-CCAase, 3 hits |
Gene3Di | 1.10.1410.20, 3 hits 3.30.460.10, 3 hits |
InterProi | View protein in InterPro IPR018952, 2-5-oligoAdlate_synth_1_dom2/C IPR038121, 2-5-oligoAdlate_synth_2_sf IPR026774, 2-5A_synthase IPR006117, 2-5OAS_C_CS IPR043518, 2-5OAS_N_CS IPR006116, NT_2-5OAS_ClassI-CCAase IPR043519, NT_sf IPR002934, Polymerase_NTP_transf_dom |
PANTHERi | PTHR11258, PTHR11258, 2 hits |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF10421, OAS1_C, 3 hits |
SUPFAMi | SSF81301, SSF81301, 3 hits |
PROSITEi | View protein in PROSITE PS00832, 25A_SYNTH_1, 1 hit PS00833, 25A_SYNTH_2, 2 hits PS50152, 25A_SYNTH_3, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8VI93-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLFHTPAGA LDKLVAHNLH PAPEFTAAVR GALGSLNITL QQHRARGSQR
60 70 80 90 100
PRVIRIAKGG AYARGTALRG GTDVELVIFL DCFQSFGDQK TCHSETLGAM
110 120 130 140 150
RMLLESWGGH PGPGLTFEFS QSKASRILQF RLASADGEHW IDVSLVPAFD
160 170 180 190 200
VLGQPRSGVK PTPNVYSSLL SSHCQAGEYS ACFTEPRKNF VNTRPAKLKN
210 220 230 240 250
LILLVKHWYH QVQTRAVRAT LPPSYALELL TIFAWEQGCG KDSFSLAQGL
260 270 280 290 300
RTVLALIQHS KYLCIFWTEN YGFEDPAVGE FLRRQLKRPR PVILDPADPT
310 320 330 340 350
WDVGNGTAWR WDVLAQEAES SFSQQCFKQA SGVLVQPWEG PGLPRAGILD
360 370 380 390 400
LGHPIYQGPN QALEDNKGHL AVQSKERSQK PSNSAPGFPE AATKIPAMPN
410 420 430 440 450
PSANKTRKIR KKAAHPKTVQ EAALDSISSH VRITQSTASS HMPPDRSSIS
460 470 480 490 500
TAGSRMSPDL SQIPSKDLDC FIQDHLRPSP QFQQQVKQAI DAILCCLREK
510 520 530 540 550
SVYKVLRVSK GGSFGRGTDL RGSCDVELVI FYKTLGDFKG QKPHQAEILR
560 570 580 590 600
DMQAQLRHWC QNPVPGLSLQ FIEQKPNALQ LQLASTDLSN RVDLSVLPAF
610 620 630 640 650
DAVGPLKSGT KPQPQVYSSL LSSGCQAGEH AACFAELRRN FINTCPPKLK
660 670 680 690 700
SLMLLVKHWY RQVVTRYKGG EAAGDAPPPA YALELLTIFA WEQGCGEQKF
710 720 730 740 750
SLAEGLRTIL RLIQQHQSLC IYWTVNYSVQ DPAIRAHLLC QLRKARPLVL
760 770 780 790 800
DPADPTWNVG QGDWKLLAQE AAALGSQVCL QSGDGTLVPP WDVTPALLHQ
810 820 830 840 850
TLAEDLDKFI SEFLQPNRHF LTQVKRAVDT ICSFLKENCF RNSTIKVLKV
860 870 880 890 900
VKGGSSAKGT ALQGRSDADL VVFLSCFRQF SEQGSHRAEI ISEIQAHLEA
910 920 930 940 950
CQQMHSFDVK FEVSKRKNPR VLSFTLTSQT LLDQSVDFDV LPAFDALGQL
960 970 980 990 1000
RSGSRPDPRV YTDLIHSCSN AGEFSTCFTE LQRDFITSRP TKLKSLIRLV
1010 1020 1030 1040 1050
KYWYQQCNKT IKGKGSLPPQ HGLELLTVYA WEQGGQNPQF NMAEGFRTVL
1060 1070 1080 1090 1100
ELIVQYRQLC VYWTINYSAE DKTIGDFLKM QLRKPRPVIL DPADPTGNLG
1110 1120 1130
HNARWDLLAK EATVYASALC CVDRDGNPIK PWPVKAAV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7CXR7 | F7CXR7_MOUSE | 2'-5'-oligoadenylate synthase 3 | Oas3 | 131 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 215 | R → Q in AAM47556 (PubMed:12080145).Curated | 1 | |
Sequence conflicti | 733 | A → T in AAM47556 (PubMed:12080145).Curated | 1 | |
Sequence conflicti | 897 | H → Q in AAM47556 (PubMed:12080145).Curated | 1 | |
Sequence conflicti | 897 | H → Q in EDL19752 (Ref. 4) Curated | 1 | |
Sequence conflicti | 897 | H → Q in AAI41056 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 904 | M → T in AAM47556 (PubMed:12080145).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB067534 mRNA Translation: BAB84134.1 AF453830 mRNA Translation: AAM47556.1 AC115937 Genomic DNA No translation available. CH466529 Genomic DNA Translation: EDL19752.1 BC141055 mRNA Translation: AAI41056.1 |
CCDSi | CCDS39242.1 |
RefSeqi | NP_660261.1, NM_145226.2 |
Genome annotation databases
Ensembli | ENSMUST00000044833; ENSMUSP00000035588; ENSMUSG00000032661 |
GeneIDi | 246727 |
KEGGi | mmu:246727 |
UCSCi | uc008zhz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB067534 mRNA Translation: BAB84134.1 AF453830 mRNA Translation: AAM47556.1 AC115937 Genomic DNA No translation available. CH466529 Genomic DNA Translation: EDL19752.1 BC141055 mRNA Translation: AAI41056.1 |
CCDSi | CCDS39242.1 |
RefSeqi | NP_660261.1, NM_145226.2 |
3D structure databases
SMRi | Q8VI93 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 232935, 6 interactors |
STRINGi | 10090.ENSMUSP00000035588 |
PTM databases
iPTMneti | Q8VI93 |
PhosphoSitePlusi | Q8VI93 |
SwissPalmi | Q8VI93 |
Proteomic databases
EPDi | Q8VI93 |
jPOSTi | Q8VI93 |
MaxQBi | Q8VI93 |
PaxDbi | Q8VI93 |
PeptideAtlasi | Q8VI93 |
PRIDEi | Q8VI93 |
Protocols and materials databases
Antibodypediai | 31208, 186 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044833; ENSMUSP00000035588; ENSMUSG00000032661 |
GeneIDi | 246727 |
KEGGi | mmu:246727 |
UCSCi | uc008zhz.1, mouse |
Organism-specific databases
CTDi | 4940 |
MGIi | MGI:2180850, Oas3 |
Phylogenomic databases
eggNOGi | ENOG502S649, Eukaryota |
GeneTreei | ENSGT00510000046406 |
HOGENOMi | CLU_287245_0_0_1 |
InParanoidi | Q8VI93 |
OMAi | CNKMPKG |
OrthoDBi | 611234at2759 |
PhylomeDBi | Q8VI93 |
TreeFami | TF329749 |
Miscellaneous databases
BioGRID-ORCSi | 246727, 0 hits in 17 CRISPR screens |
ChiTaRSi | Oas3, mouse |
PROi | PR:Q8VI93 |
RNActi | Q8VI93, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032661, Expressed in granulocyte and 72 other tissues |
Genevisiblei | Q8VI93, MM |
Family and domain databases
CDDi | cd05400, NT_2-5OAS_ClassI-CCAase, 3 hits |
Gene3Di | 1.10.1410.20, 3 hits 3.30.460.10, 3 hits |
InterProi | View protein in InterPro IPR018952, 2-5-oligoAdlate_synth_1_dom2/C IPR038121, 2-5-oligoAdlate_synth_2_sf IPR026774, 2-5A_synthase IPR006117, 2-5OAS_C_CS IPR043518, 2-5OAS_N_CS IPR006116, NT_2-5OAS_ClassI-CCAase IPR043519, NT_sf IPR002934, Polymerase_NTP_transf_dom |
PANTHERi | PTHR11258, PTHR11258, 2 hits |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF10421, OAS1_C, 3 hits |
SUPFAMi | SSF81301, SSF81301, 3 hits |
PROSITEi | View protein in PROSITE PS00832, 25A_SYNTH_1, 1 hit PS00833, 25A_SYNTH_2, 2 hits PS50152, 25A_SYNTH_3, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OAS3_MOUSE | |
Accessioni | Q8VI93Primary (citable) accession number: Q8VI93 Secondary accession number(s): B9EIU4, Q8K4D2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families