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Protein

Transcription factor Sp7

Gene

Sp7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri291 – 315C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri321 – 345C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri351 – 373C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Sp7
Alternative name(s):
C22
Zinc finger protein osterix
Gene namesi
Name:Sp7
Synonyms:Osx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2153568 Sp7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Death in the immediate postnatal period due to difficulty in breathing. Mice rapidly become cyanotic and die within 15 min of birth. New-born homozygous show severe inward bending of forelimbs and hindlimbs. They develop a normal cartilage skeleton but fail to form bone and to express osteoblast-specific marker genes. In endochondral skeletal elements, mesenchymal cells together with osteoclasts and blood vessels, invade the mineralized cartilage matrix.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471511 – 428Transcription factor Sp7Add BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ8VI67
PRIDEiQ8VI67

PTM databases

iPTMnetiQ8VI67
PhosphoSitePlusiQ8VI67

Expressioni

Tissue specificityi

Osteoblast/chondrocyte specific.1 Publication

Inductioni

In response to ascorbic acid induction, expression is activated by NFE2L1 in osteoblasts.1 Publication

Gene expression databases

BgeeiENSMUSG00000060284 Expressed in 100 organ(s), highest expression level in hindlimb long bone
CleanExiMM_SP7
ExpressionAtlasiQ8VI67 baseline and differential
GenevisibleiQ8VI67 MM

Interactioni

Subunit structurei

Interacts with RIOX1; the interaction is direct and inhibits transcription activator activity.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228354, 7 interactors
DIPiDIP-46090N
IntActiQ8VI67, 6 interactors
MINTiQ8VI67
STRINGi10090.ENSMUSP00000077596

Structurei

3D structure databases

ProteinModelPortaliQ8VI67
SMRiQ8VI67
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri291 – 315C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri321 – 345C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri351 – 373C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118984
HOGENOMiHOG000231067
HOVERGENiHBG036899
InParanoidiQ8VI67
KOiK09197
OMAiEEAHYGS
OrthoDBiEOG091G0HX6
PhylomeDBiQ8VI67
TreeFamiTF350150

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8VI67-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD
60 70 80 90 100
LSAPKTMGDA YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT
110 120 130 140 150
GAQDPGLLVP KGHSSSDCLP SVYTSLDMTH PYGSWYKAGI HAGISPGPGN
160 170 180 190 200
TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS TGPAQPPLNP QLPTYPSDFA
210 220 230 240 250
PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS GAAKPPRGAG
260 270 280 290 300
TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK
310 320 330 340 350
VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK
360 370 380 390 400
FTCLLCSKRF TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN
410 420
QPPRSSTSPA PPEKAHGGSP EQSNLLEI
Length:428
Mass (Da):44,718
Last modified:March 1, 2002 - v1
Checksum:iB794988958743586
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5RM08Q5RM08_MOUSE
Osterix
Sp7
410Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64P → L in BAC36263 (PubMed:16141072).Curated1
Sequence conflicti130H → Y in BAC36774 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF184902 mRNA Translation: AAL60067.1
AK032521 mRNA Translation: BAC27908.1
AK076229 mRNA Translation: BAC36263.1
AK077375 mRNA Translation: BAC36774.1
CCDSiCCDS37228.1
RefSeqiNP_001335134.1, NM_001348205.1
NP_569725.1, NM_130458.4
XP_006520582.1, XM_006520519.3
UniGeneiMm.263284

Genome annotation databases

EnsembliENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284
GeneIDi170574
KEGGimmu:170574
UCSCiuc007xvm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF184902 mRNA Translation: AAL60067.1
AK032521 mRNA Translation: BAC27908.1
AK076229 mRNA Translation: BAC36263.1
AK077375 mRNA Translation: BAC36774.1
CCDSiCCDS37228.1
RefSeqiNP_001335134.1, NM_001348205.1
NP_569725.1, NM_130458.4
XP_006520582.1, XM_006520519.3
UniGeneiMm.263284

3D structure databases

ProteinModelPortaliQ8VI67
SMRiQ8VI67
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228354, 7 interactors
DIPiDIP-46090N
IntActiQ8VI67, 6 interactors
MINTiQ8VI67
STRINGi10090.ENSMUSP00000077596

PTM databases

iPTMnetiQ8VI67
PhosphoSitePlusiQ8VI67

Proteomic databases

PaxDbiQ8VI67
PRIDEiQ8VI67

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284
GeneIDi170574
KEGGimmu:170574
UCSCiuc007xvm.1 mouse

Organism-specific databases

CTDi121340
MGIiMGI:2153568 Sp7

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118984
HOGENOMiHOG000231067
HOVERGENiHBG036899
InParanoidiQ8VI67
KOiK09197
OMAiEEAHYGS
OrthoDBiEOG091G0HX6
PhylomeDBiQ8VI67
TreeFamiTF350150

Miscellaneous databases

PROiPR:Q8VI67
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060284 Expressed in 100 organ(s), highest expression level in hindlimb long bone
CleanExiMM_SP7
ExpressionAtlasiQ8VI67 baseline and differential
GenevisibleiQ8VI67 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSP7_MOUSE
AccessioniPrimary (citable) accession number: Q8VI67
Secondary accession number(s): Q8C5R3, Q8C6A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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