UniProtKB - Q8VI67 (SP7_MOUSE)
Protein
Transcription factor Sp7
Gene
Sp7
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional activator essential for osteoblast differentiation (PubMed:11792318, PubMed:17510056, PubMed:30026585). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (PubMed:11792318, PubMed:17510056).3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 291 – 315 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 321 – 345 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 351 – 373 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DEAD/H-box RNA helicase binding Source: BHF-UCL
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- hematopoietic stem cell differentiation Source: MGI
- osteoblast differentiation Source: MGI
- positive regulation of stem cell differentiation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor Sp7Alternative name(s): C22 Zinc finger protein osterix |
Gene namesi | Name:Sp7 Synonyms:Osx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2153568, Sp7 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nuclear chromatin Source: GO_Central
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Death in the immediate postnatal period due to difficulty in breathing. Mice rapidly become cyanotic and die within 15 min of birth. New-born homozygous show severe inward bending of forelimbs and hindlimbs. They develop a normal cartilage skeleton but fail to form bone and to express osteoblast-specific marker genes. In endochondral skeletal elements, mesenchymal cells together with osteoclasts and blood vessels, invade the mineralized cartilage matrix.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 41 – 46 | KGGTKK → RGGTRR: Decreased propionylation. 1 Publication | 6 | |
Mutagenesisi | 368 | K → R: Decreased propionylation, leading to increased transcription activator activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047151 | 1 – 428 | Transcription factor Sp7Add BLAST | 428 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 41 | N6-propionyllysine1 Publication | 1 | |
Modified residuei | 45 | N6-propionyllysine1 Publication | 1 | |
Cross-linki | 55 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 227 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 358 | N6-propionyllysine1 Publication | 1 | |
Modified residuei | 368 | N6-propionyllysine1 Publication | 1 |
Post-translational modificationi
Propionylated (PubMed:30026585). Depropionylation at Lys-368 by SIRT7 activates transcription factor activity and positively regulates bone formation by osteoblasts (PubMed:30026585).1 Publication
Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q8VI67 |
PRIDEi | Q8VI67 |
PTM databases
iPTMneti | Q8VI67 |
PhosphoSitePlusi | Q8VI67 |
Expressioni
Tissue specificityi
Osteoblast/chondrocyte specific.1 Publication
Inductioni
In response to ascorbic acid induction, expression is activated by NFE2L1 in osteoblasts.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000060284, Expressed in hindlimb long bone and 122 other tissues |
ExpressionAtlasi | Q8VI67, baseline and differential |
Genevisiblei | Q8VI67, MM |
Interactioni
Subunit structurei
Interacts with RIOX1; the interaction is direct and inhibits transcription activator activity.
1 PublicationBinary interactionsi
Hide detailsQ8VI67
With | #Exp. | IntAct |
---|---|---|
Riox1 [Q9JJF3] | 3 | EBI-7608836,EBI-7608809 |
RIOX1 [Q9H6W3] from Homo sapiens. | 6 | EBI-7608836,EBI-2513645 |
GO - Molecular functioni
- DEAD/H-box RNA helicase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 228354, 7 interactors |
DIPi | DIP-46090N |
IntActi | Q8VI67, 6 interactors |
MINTi | Q8VI67 |
STRINGi | 10090.ENSMUSP00000077596 |
Miscellaneous databases
RNActi | Q8VI67, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 153 – 161 | 9aaTADBy similarity | 9 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.By similarity
Sequence similaritiesi
Belongs to the Sp1 C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 291 – 315 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 321 – 345 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 351 – 373 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161293 |
HOGENOMi | CLU_019484_4_2_1 |
InParanoidi | Q8VI67 |
OMAi | PTRSSCD |
OrthoDBi | 664813at2759 |
PhylomeDBi | Q8VI67 |
TreeFami | TF350150 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8VI67-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD
60 70 80 90 100
LSAPKTMGDA YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT
110 120 130 140 150
GAQDPGLLVP KGHSSSDCLP SVYTSLDMTH PYGSWYKAGI HAGISPGPGN
160 170 180 190 200
TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS TGPAQPPLNP QLPTYPSDFA
210 220 230 240 250
PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS GAAKPPRGAG
260 270 280 290 300
TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK
310 320 330 340 350
VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK
360 370 380 390 400
FTCLLCSKRF TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN
410 420
QPPRSSTSPA PPEKAHGGSP EQSNLLEI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5RM08 | Q5RM08_MOUSE | Osterix | Sp7 | 410 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 | P → L in BAC36263 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 130 | H → Y in BAC36774 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF184902 mRNA Translation: AAL60067.1 AK032521 mRNA Translation: BAC27908.1 AK076229 mRNA Translation: BAC36263.1 AK077375 mRNA Translation: BAC36774.1 |
CCDSi | CCDS37228.1 |
RefSeqi | NP_001335134.1, NM_001348205.1 NP_569725.1, NM_130458.4 XP_006520582.1, XM_006520519.3 |
Genome annotation databases
Ensembli | ENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284 |
GeneIDi | 170574 |
KEGGi | mmu:170574 |
UCSCi | uc007xvm.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF184902 mRNA Translation: AAL60067.1 AK032521 mRNA Translation: BAC27908.1 AK076229 mRNA Translation: BAC36263.1 AK077375 mRNA Translation: BAC36774.1 |
CCDSi | CCDS37228.1 |
RefSeqi | NP_001335134.1, NM_001348205.1 NP_569725.1, NM_130458.4 XP_006520582.1, XM_006520519.3 |
3D structure databases
SMRi | Q8VI67 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228354, 7 interactors |
DIPi | DIP-46090N |
IntActi | Q8VI67, 6 interactors |
MINTi | Q8VI67 |
STRINGi | 10090.ENSMUSP00000077596 |
PTM databases
iPTMneti | Q8VI67 |
PhosphoSitePlusi | Q8VI67 |
Proteomic databases
PaxDbi | Q8VI67 |
PRIDEi | Q8VI67 |
Protocols and materials databases
Antibodypediai | 3133, 169 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284 |
GeneIDi | 170574 |
KEGGi | mmu:170574 |
UCSCi | uc007xvm.1, mouse |
Organism-specific databases
CTDi | 121340 |
MGIi | MGI:2153568, Sp7 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161293 |
HOGENOMi | CLU_019484_4_2_1 |
InParanoidi | Q8VI67 |
OMAi | PTRSSCD |
OrthoDBi | 664813at2759 |
PhylomeDBi | Q8VI67 |
TreeFami | TF350150 |
Miscellaneous databases
BioGRID-ORCSi | 170574, 1 hit in 17 CRISPR screens |
PROi | PR:Q8VI67 |
RNActi | Q8VI67, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000060284, Expressed in hindlimb long bone and 122 other tissues |
ExpressionAtlasi | Q8VI67, baseline and differential |
Genevisiblei | Q8VI67, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SP7_MOUSE | |
Accessioni | Q8VI67Primary (citable) accession number: Q8VI67 Secondary accession number(s): Q8C5R3, Q8C6A7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families