UniProtKB - Q8VHW5 (CCG8_RAT)
Protein
Voltage-dependent calcium channel gamma-8 subunit
Gene
Cacng8
Organism
Rattus norvegicus (Rat)
Status
Functioni
Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit (By similarity). Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.By similarity2 Publications
GO - Molecular functioni
- calcium channel regulator activity Source: UniProtKB
- channel regulator activity Source: GO_Central
- ionotropic glutamate receptor binding Source: RGD
- protein phosphatase 2B binding Source: RGD
- voltage-gated calcium channel activity Source: GO_Central
GO - Biological processi
- neurotransmitter receptor internalization Source: GO_Central
- neurotransmitter receptor transport, postsynaptic endosome to lysosome Source: GO_Central
- positive regulation of AMPA receptor activity Source: RGD
- positive regulation of synaptic transmission, glutamatergic Source: GO_Central
- postsynaptic neurotransmitter receptor diffusion trapping Source: GO_Central
- regulation of AMPA receptor activity Source: UniProtKB
- regulation of postsynaptic membrane neurotransmitter receptor levels Source: RGD
- transmission of nerve impulse Source: GO_Central
Keywordsi
Molecular function | Calcium channel, Ion channel, Voltage-gated channel |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-RNO-399719, Trafficking of AMPA receptors R-RNO-5576892, Phase 0 - rapid depolarisation R-RNO-5576893, Phase 2 - plateau phase R-RNO-5682910, LGI-ADAM interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent calcium channel gamma-8 subunitAlternative name(s): Neuronal voltage-gated calcium channel gamma-8 subunit Transmembrane AMPAR regulatory protein gamma-8 Short name: TARP gamma-8 |
Gene namesi | Name:Cacng8 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628808, Cacng8 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Curated
- postsynaptic density membrane By similarity
Plasma Membrane
- AMPA glutamate receptor complex Source: UniProtKB
- dendrite membrane Source: RGD
- integral component of postsynaptic density membrane Source: SynGO
- L-type voltage-gated calcium channel complex Source: UniProtKB
- postsynaptic density membrane Source: GO_Central
- postsynaptic membrane Source: RGD
Other locations
- glutamatergic synapse Source: SynGO
- postsynaptic density Source: RGD
- Schaffer collateral - CA1 synapse Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 19 – 39 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 127 – 147 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 157 – 177 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 207 – 227 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 318 – 338 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000164692 | 1 – 421 | Voltage-dependent calcium channel gamma-8 subunitAdd BLAST | 421 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 251 | PhosphoserineCombined sources | 1 | |
Modified residuei | 254 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Palmitoylated (PubMed:23687301). Probably palmitoylated by ZDHHC3 and ZDHHC7 (PubMed:23687301).1 Publication
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q8VHW5 |
PRIDEi | Q8VHW5 |
PTM databases
iPTMneti | Q8VHW5 |
PhosphoSitePlusi | Q8VHW5 |
SwissPalmi | Q8VHW5 |
Interactioni
Subunit structurei
Interacts with CACNA1C.
Identified in a complex with the L-type calcium channel subunits CACNA1C, CACNA2D1 and either CACNB1 or CACNB2 (By similarity). Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs).
Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5 and CACNG7.
Interacts with CNIH2 (By similarity).
By similarityBinary interactionsi
Hide detailsQ8VHW5
With | #Exp. | IntAct |
---|---|---|
Ppp3ca [P63329] | 6 | EBI-9086576,EBI-7022944 |
PPP3CA [Q08209] from Homo sapiens. | 2 | EBI-9086576,EBI-352922 |
GO - Molecular functioni
- ionotropic glutamate receptor binding Source: RGD
- protein phosphatase 2B binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 250845, 2 interactors |
CORUMi | Q8VHW5 |
IntActi | Q8VHW5, 8 interactors |
MINTi | Q8VHW5 |
STRINGi | 10116.ENSRNOP00000019300 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 245 – 360 | Gly-richAdd BLAST | 116 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUEC, Eukaryota |
InParanoidi | Q8VHW5 |
OrthoDBi | 1117127at2759 |
PhylomeDBi | Q8VHW5 |
Family and domain databases
InterProi | View protein in InterPro IPR004031, PMP22/EMP/MP20/Claudin IPR008372, VDCC_g8su IPR008368, VDCC_gsu |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01792, VDCCGAMMA PR01796, VDCCGAMMA8 |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8VHW5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESLKRWNEE RGLWCEKGVQ VLLTTIGAFA AFGLMTIAIS TDYWLYTRAL
60 70 80 90 100
ICNTTNLTAG DDGPPHRGGS GSSEKKDPGG LTHSGLWRIC CLEGLKRGVC
110 120 130 140 150
VKINHFPEDT DYDHDSAEYL LRVVRASSIF PILSAILLLL GGVCVAASRV
160 170 180 190 200
YKSKRNIILG AGILFVAAGL SNIIGVIVYI SANAGEPGPK RDEEKKNHYS
210 220 230 240 250
YGWSFYFGGL SFILAEVIGV LAVNIYIERS REAHCQSRSD LLKAGGGAGG
260 270 280 290 300
SGGSGPSAIL RLPSYRFRYR RRSRSSSRGS SEASPSRDAS PGGPGGPGFA
310 320 330 340 350
STDISMYTLS RDPSKGSVAA GLASAGGGGG GAGVGAYGGA AGAAGGGGTG
360 370 380 390 400
SERDRGSSAG FLTLHNAFPK EAASGVTVTV TGPPAAPAPA PPAPAAPAPG
410 420
TLSKEAAASN TNTLNRKTTP V
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1M7K7 | F1M7K7_RAT | Voltage-dependent calcium channel g... | Cacng8 | 421 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF361346 mRNA Translation: AAL50041.1 |
RefSeqi | NP_542427.1, NM_080696.2 |
Genome annotation databases
GeneIDi | 140729 |
KEGGi | rno:140729 |
UCSCi | RGD:628808, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF361346 mRNA Translation: AAL50041.1 |
RefSeqi | NP_542427.1, NM_080696.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6QKC | electron microscopy | 4.10 | I/J | 2-417 | [»] | |
6QKZ | electron microscopy | 6.30 | I/J | 2-417 | [»] | |
SMRi | Q8VHW5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250845, 2 interactors |
CORUMi | Q8VHW5 |
IntActi | Q8VHW5, 8 interactors |
MINTi | Q8VHW5 |
STRINGi | 10116.ENSRNOP00000019300 |
PTM databases
iPTMneti | Q8VHW5 |
PhosphoSitePlusi | Q8VHW5 |
SwissPalmi | Q8VHW5 |
Proteomic databases
PaxDbi | Q8VHW5 |
PRIDEi | Q8VHW5 |
Protocols and materials databases
ABCDi | Q8VHW5, 1 sequenced antibody |
Genome annotation databases
GeneIDi | 140729 |
KEGGi | rno:140729 |
UCSCi | RGD:628808, rat |
Organism-specific databases
CTDi | 59283 |
RGDi | 628808, Cacng8 |
Phylogenomic databases
eggNOGi | ENOG502QUEC, Eukaryota |
InParanoidi | Q8VHW5 |
OrthoDBi | 1117127at2759 |
PhylomeDBi | Q8VHW5 |
Enzyme and pathway databases
Reactomei | R-RNO-399719, Trafficking of AMPA receptors R-RNO-5576892, Phase 0 - rapid depolarisation R-RNO-5576893, Phase 2 - plateau phase R-RNO-5682910, LGI-ADAM interactions |
Miscellaneous databases
PROi | PR:Q8VHW5 |
Family and domain databases
InterProi | View protein in InterPro IPR004031, PMP22/EMP/MP20/Claudin IPR008372, VDCC_g8su IPR008368, VDCC_gsu |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01792, VDCCGAMMA PR01796, VDCCGAMMA8 |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCG8_RAT | |
Accessioni | Q8VHW5Primary (citable) accession number: Q8VHW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2002 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families