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Protein

Transcriptional repressor p66-beta

Gene

Gatad2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A (By similarity). Targets MBD3 to discrete loci in the nucleus (By similarity). Plays a role in synapse development (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri415 – 468GATA-typePROSITE-ProRule annotationAdd BLAST54

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: GO_Central

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214815 HDACs deacetylate histones
R-MMU-6804758 Regulation of TP53 Activity through Acetylation
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-8943724 Regulation of PTEN gene transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor p66-beta
Alternative name(s):
GATA zinc finger domain-containing protein 2B
p66/p68
Gene namesi
Name:Gatad2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2443225 Gatad2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835031 – 594Transcriptional repressor p66-betaAdd BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Cross-linki33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki66Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki98Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei121PhosphothreonineCombined sources1
Modified residuei123PhosphoserineCombined sources1
Modified residuei130PhosphoserineCombined sources1
Modified residuei135PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Cross-linki148Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei209PhosphoserineBy similarity1
Cross-linki282Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei334PhosphoserineBy similarity1
Modified residuei339PhosphoserineCombined sources1
Modified residuei341PhosphoserineCombined sources1
Cross-linki354Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki455Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki468Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei487PhosphoserineBy similarity1
Cross-linki499Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8VHR5
PaxDbiQ8VHR5
PeptideAtlasiQ8VHR5
PRIDEiQ8VHR5

PTM databases

iPTMnetiQ8VHR5
PhosphoSitePlusiQ8VHR5

Expressioni

Gene expression databases

BgeeiENSMUSG00000042390 Expressed in 255 organ(s), highest expression level in secondary oocyte
CleanExiMM_GATAD2B
ExpressionAtlasiQ8VHR5 baseline and differential
GenevisibleiQ8VHR5 MM

Interactioni

Subunit structurei

Binds MBD2 and MBD3. Interaction with MBD2 is required for the enhancement of MBD2-mediated repression and for targeting to the chromatin (By similarity). Component of the MeCP1 histone deacetylase complex (By similarity). Interacts with histone tails, including that of histones H2A, H2B, H3 and H4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Nr4a1P128132EBI-3043880,EBI-10896863

Protein-protein interaction databases

BioGridi230858, 9 interactors
ComplexPortaliCPX-953 MBD2/NuRD nucleosome remodeling and deacetylase complex
CPX-954 MBD3/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ8VHR5
DIPiDIP-62049N
IntActiQ8VHR5, 8 interactors
MINTiQ8VHR5
STRINGi10090.ENSMUSP00000041370

Structurei

3D structure databases

ProteinModelPortaliQ8VHR5
SMRiQ8VHR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 191CR1Add BLAST26
Regioni341 – 481CR2Add BLAST141

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili141 – 195Sequence analysisAdd BLAST55
Coiled coili450 – 483Sequence analysisAdd BLAST34

Domaini

Both CR1 and CR2 regions are required for speckled nuclear localization.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri415 – 468GATA-typePROSITE-ProRule annotationAdd BLAST54

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IQ4W Eukaryota
ENOG410ZCNE LUCA
GeneTreeiENSGT00390000004097
HOGENOMiHOG000074070
HOVERGENiHBG053401
InParanoidiQ8VHR5
OMAiMNPTINY
OrthoDBiEOG091G0HRN
PhylomeDBiQ8VHR5
TreeFamiTF321369

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR032346 P66_CC
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00320 GATA, 1 hit
PF16563 P66_CC, 1 hit
PROSITEiView protein in PROSITE
PS50114 GATA_ZN_FINGER_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8VHR5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDRMTEDALR LNLLKRSLDP ADERDDVLAK RLKMEGHEAM ERLKMLALLK
60 70 80 90 100
RKDLANLEVP HELPTKQDGS GVKGYEEKLN GNLRPHGDNN RTAGRPGKEN
110 120 130 140 150
INDEPVDMSA RRSEPDRGRL TPSPDIIVLS DNEASSPRSS SRMEERLKAA
160 170 180 190 200
NLEMFKGKGM EERQQLIKQL RDELRLEEAR LVLLKKLRQS QLQKENVVQK
210 220 230 240 250
TPVVQNAASI VQPSPAHVGQ QGLSKLPSRP GAQGIEPQNM RTLQGHSVIR
260 270 280 290 300
SATNTTLPHM LMSQRVIAPN PAQLQGQRGP PKPGIVRTTT PNMNPAISYQ
310 320 330 340 350
PQSSSSVPCQ RTTSSAIYMN LASHIQPGTV NRVSSPLPSP SAMSDAANSQ
360 370 380 390 400
AAAKLALRKQ LEKTLLEIPP PKPPAPLLHF LPSAANSEFI YMVGLEEVVQ
410 420 430 440 450
SVIDSQGKNC ASLLRVEPFV CAQCRTDFTP HWKQEKNGKI LCEQCMTSNQ
460 470 480 490 500
KKALKAEHTN RLKNAFVKAL QQEQEIEQRL QQQAALSPTT APAVSSVSKQ
510 520 530 540 550
ETIMRHHTLR QAPQPQSSLQ RGIPTSARSM LSNFAQAPQL SVPGGLLGMP
560 570 580 590
GVNIAYLNTG IGGHKAPSLA DRQREYLLDM IPPRSISQSI SGQK
Length:594
Mass (Da):65,411
Last modified:March 1, 2002 - v1
Checksum:iC57330259EADAAE6
GO
Isoform 2 (identifier: Q8VHR5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     475-485: EIEQRLQQQAA → VRTLTPTCTVI
     486-594: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:485
Mass (Da):53,734
Checksum:i319D9E907558D734
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A1L3S7A1L3S7_MOUSE
Gatad2b protein
Gatad2b
578Annotation score:
A0A0U1RNM2A0A0U1RNM2_MOUSE
Transcriptional repressor p66-beta
Gatad2b
263Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010930475 – 485EIEQRLQQQAA → VRTLTPTCTVI in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_010931486 – 594Missing in isoform 2. 1 PublicationAdd BLAST109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411837 mRNA Translation: AAL39081.1
AK041594 mRNA Translation: BAC30997.1
CCDSiCCDS17526.1 [Q8VHR5-1]
RefSeqiNP_647465.1, NM_139304.1 [Q8VHR5-1]
XP_006501422.2, XM_006501359.3 [Q8VHR5-1]
XP_006501424.1, XM_006501361.2 [Q8VHR5-1]
UniGeneiMm.270999

Genome annotation databases

EnsembliENSMUST00000049382; ENSMUSP00000041370; ENSMUSG00000042390 [Q8VHR5-1]
ENSMUST00000199607; ENSMUSP00000142617; ENSMUSG00000042390 [Q8VHR5-1]
ENSMUST00000199754; ENSMUSP00000142514; ENSMUSG00000042390 [Q8VHR5-1]
GeneIDi229542
KEGGimmu:229542
UCSCiuc008qca.1 mouse [Q8VHR5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411837 mRNA Translation: AAL39081.1
AK041594 mRNA Translation: BAC30997.1
CCDSiCCDS17526.1 [Q8VHR5-1]
RefSeqiNP_647465.1, NM_139304.1 [Q8VHR5-1]
XP_006501422.2, XM_006501359.3 [Q8VHR5-1]
XP_006501424.1, XM_006501361.2 [Q8VHR5-1]
UniGeneiMm.270999

3D structure databases

ProteinModelPortaliQ8VHR5
SMRiQ8VHR5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230858, 9 interactors
ComplexPortaliCPX-953 MBD2/NuRD nucleosome remodeling and deacetylase complex
CPX-954 MBD3/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ8VHR5
DIPiDIP-62049N
IntActiQ8VHR5, 8 interactors
MINTiQ8VHR5
STRINGi10090.ENSMUSP00000041370

PTM databases

iPTMnetiQ8VHR5
PhosphoSitePlusiQ8VHR5

Proteomic databases

EPDiQ8VHR5
PaxDbiQ8VHR5
PeptideAtlasiQ8VHR5
PRIDEiQ8VHR5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049382; ENSMUSP00000041370; ENSMUSG00000042390 [Q8VHR5-1]
ENSMUST00000199607; ENSMUSP00000142617; ENSMUSG00000042390 [Q8VHR5-1]
ENSMUST00000199754; ENSMUSP00000142514; ENSMUSG00000042390 [Q8VHR5-1]
GeneIDi229542
KEGGimmu:229542
UCSCiuc008qca.1 mouse [Q8VHR5-1]

Organism-specific databases

CTDi57459
MGIiMGI:2443225 Gatad2b

Phylogenomic databases

eggNOGiENOG410IQ4W Eukaryota
ENOG410ZCNE LUCA
GeneTreeiENSGT00390000004097
HOGENOMiHOG000074070
HOVERGENiHBG053401
InParanoidiQ8VHR5
OMAiMNPTINY
OrthoDBiEOG091G0HRN
PhylomeDBiQ8VHR5
TreeFamiTF321369

Enzyme and pathway databases

ReactomeiR-MMU-3214815 HDACs deacetylate histones
R-MMU-6804758 Regulation of TP53 Activity through Acetylation
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-8943724 Regulation of PTEN gene transcription

Miscellaneous databases

ChiTaRSiGatad2b mouse
PROiPR:Q8VHR5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042390 Expressed in 255 organ(s), highest expression level in secondary oocyte
CleanExiMM_GATAD2B
ExpressionAtlasiQ8VHR5 baseline and differential
GenevisibleiQ8VHR5 MM

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR032346 P66_CC
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00320 GATA, 1 hit
PF16563 P66_CC, 1 hit
PROSITEiView protein in PROSITE
PS50114 GATA_ZN_FINGER_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP66B_MOUSE
AccessioniPrimary (citable) accession number: Q8VHR5
Secondary accession number(s): Q8C9Q3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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