UniProtKB - Q8VHR5 (P66B_MOUSE)
Transcriptional repressor p66-beta
Gatad2b
Functioni
Transcriptional repressor (By similarity).
Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A (By similarity).
Targets MBD3 to discrete loci in the nucleus (By similarity).
Plays a role in synapse development (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 415 – 468 | GATA-typePROSITE-ProRule annotationAdd BLAST | 54 |
GO - Molecular functioni
- sequence-specific DNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- chromatin remodeling Source: MGI
- histone deacetylation Source: MGI
- negative regulation of transcription, DNA-templated Source: ComplexPortal
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- positive regulation of transcription, DNA-templated Source: ComplexPortal
- regulation of cell fate specification Source: ComplexPortal
- regulation of stem cell differentiation Source: ComplexPortal
Keywordsi
Molecular function | Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3214815, HDACs deacetylate histones R-MMU-6804758, Regulation of TP53 Activity through Acetylation R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-8943724, Regulation of PTEN gene transcription |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional repressor p66-betaAlternative name(s): GATA zinc finger domain-containing protein 2B p66/p68 |
Gene namesi | Name:Gatad2b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443225, Gatad2b |
VEuPathDBi | HostDB:ENSMUSG00000042390 |
Subcellular locationi
Nucleus
- Nucleus speckle By similarity
Note: Speckled nuclear localization requires both CR1 and CR2 regions.By similarity
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- NuRD complex Source: GO_Central
Other locations
- chromosome, telomeric region Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083503 | 1 – 594 | Transcriptional repressor p66-betaAdd BLAST | 594 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 17 | PhosphoserineBy similarity | 1 | |
Cross-linki | 33 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 66 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 98 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 121 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 123 | PhosphoserineCombined sources | 1 | |
Modified residuei | 130 | PhosphoserineCombined sources | 1 | |
Modified residuei | 135 | PhosphoserineCombined sources | 1 | |
Modified residuei | 136 | PhosphoserineCombined sources | 1 | |
Cross-linki | 148 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 200 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 209 | PhosphoserineBy similarity | 1 | |
Cross-linki | 282 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 334 | PhosphoserineBy similarity | 1 | |
Modified residuei | 339 | PhosphoserineCombined sources | 1 | |
Modified residuei | 341 | PhosphoserineCombined sources | 1 | |
Cross-linki | 354 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 455 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 468 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 487 | PhosphoserineBy similarity | 1 | |
Cross-linki | 499 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8VHR5 |
jPOSTi | Q8VHR5 |
MaxQBi | Q8VHR5 |
PaxDbi | Q8VHR5 |
PeptideAtlasi | Q8VHR5 |
PRIDEi | Q8VHR5 |
ProteomicsDBi | 294313 [Q8VHR5-1] 294314 [Q8VHR5-2] |
PTM databases
iPTMneti | Q8VHR5 |
PhosphoSitePlusi | Q8VHR5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000042390, Expressed in secondary oocyte and 279 other tissues |
Genevisiblei | Q8VHR5, MM |
Interactioni
Subunit structurei
Interacts with MBD2 and MBD3 (By similarity). Interaction with MBD2 is required for the enhancement of MBD2-mediated repression and for targeting to the chromatin (By similarity).
Component of the MeCP1 histone deacetylase complex (By similarity).
Interacts with histone tails, including that of histones H2A, H2B, H3 and H4 (By similarity).
Interacts with ERCC6 (By similarity).
By similarityBinary interactionsi
Q8VHR5
With | #Exp. | IntAct |
---|---|---|
Nr4a1 [P12813] | 2 | EBI-3043880,EBI-10896863 |
Protein-protein interaction databases
BioGRIDi | 230858, 18 interactors |
ComplexPortali | CPX-953, MBD2/NuRD nucleosome remodeling and deacetylase complex CPX-954, MBD3/NuRD nucleosome remodeling and deacetylase complex |
CORUMi | Q8VHR5 |
DIPi | DIP-62049N |
IntActi | Q8VHR5, 8 interactors |
MINTi | Q8VHR5 |
STRINGi | 10090.ENSMUSP00000041370 |
Miscellaneous databases
RNActi | Q8VHR5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 62 – 143 | DisorderedSequence analysisAdd BLAST | 82 | |
Regioni | 166 – 191 | CR1; interaction with MBD2 and MBD3By similarityAdd BLAST | 26 | |
Regioni | 214 – 237 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 341 – 481 | CR2; histone tail-bindingBy similarityAdd BLAST | 141 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 141 – 195 | Sequence analysisAdd BLAST | 55 | |
Coiled coili | 450 – 483 | Sequence analysisAdd BLAST | 34 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 91 – 119 | Basic and acidic residuesSequence analysisAdd BLAST | 29 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 415 – 468 | GATA-typePROSITE-ProRule annotationAdd BLAST | 54 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG3740, Eukaryota |
GeneTreei | ENSGT00390000004097 |
InParanoidi | Q8VHR5 |
OMAi | PEERMHQ |
OrthoDBi | 885815at2759 |
PhylomeDBi | Q8VHR5 |
TreeFami | TF321369 |
Family and domain databases
Gene3Di | 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR040386, P66 IPR032346, P66_CC IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR13455, PTHR13455, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 1 hit PF16563, P66_CC, 1 hit |
PROSITEi | View protein in PROSITE PS50114, GATA_ZN_FINGER_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDRMTEDALR LNLLKRSLDP ADERDDVLAK RLKMEGHEAM ERLKMLALLK
60 70 80 90 100
RKDLANLEVP HELPTKQDGS GVKGYEEKLN GNLRPHGDNN RTAGRPGKEN
110 120 130 140 150
INDEPVDMSA RRSEPDRGRL TPSPDIIVLS DNEASSPRSS SRMEERLKAA
160 170 180 190 200
NLEMFKGKGM EERQQLIKQL RDELRLEEAR LVLLKKLRQS QLQKENVVQK
210 220 230 240 250
TPVVQNAASI VQPSPAHVGQ QGLSKLPSRP GAQGIEPQNM RTLQGHSVIR
260 270 280 290 300
SATNTTLPHM LMSQRVIAPN PAQLQGQRGP PKPGIVRTTT PNMNPAISYQ
310 320 330 340 350
PQSSSSVPCQ RTTSSAIYMN LASHIQPGTV NRVSSPLPSP SAMSDAANSQ
360 370 380 390 400
AAAKLALRKQ LEKTLLEIPP PKPPAPLLHF LPSAANSEFI YMVGLEEVVQ
410 420 430 440 450
SVIDSQGKNC ASLLRVEPFV CAQCRTDFTP HWKQEKNGKI LCEQCMTSNQ
460 470 480 490 500
KKALKAEHTN RLKNAFVKAL QQEQEIEQRL QQQAALSPTT APAVSSVSKQ
510 520 530 540 550
ETIMRHHTLR QAPQPQSSLQ RGIPTSARSM LSNFAQAPQL SVPGGLLGMP
560 570 580 590
GVNIAYLNTG IGGHKAPSLA DRQREYLLDM IPPRSISQSI SGQK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA1L3S7 | A1L3S7_MOUSE | Gatad2b protein | Gatad2b | 578 | Annotation score: | ||
A0A0U1RNM2 | A0A0U1RNM2_MOUSE | Transcriptional repressor p66-beta | Gatad2b | 263 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010930 | 475 – 485 | EIEQRLQQQAA → VRTLTPTCTVI in isoform 2. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_010931 | 486 – 594 | Missing in isoform 2. 1 PublicationAdd BLAST | 109 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF411837 mRNA Translation: AAL39081.1 AK041594 mRNA Translation: BAC30997.1 |
CCDSi | CCDS17526.1 [Q8VHR5-1] |
RefSeqi | NP_647465.1, NM_139304.1 [Q8VHR5-1] XP_006501422.2, XM_006501359.3 XP_006501424.1, XM_006501361.2 |
Genome annotation databases
Ensembli | ENSMUST00000049382; ENSMUSP00000041370; ENSMUSG00000042390 [Q8VHR5-1] ENSMUST00000199607; ENSMUSP00000142617; ENSMUSG00000042390 [Q8VHR5-1] ENSMUST00000199754; ENSMUSP00000142514; ENSMUSG00000042390 [Q8VHR5-1] |
GeneIDi | 229542 |
KEGGi | mmu:229542 |
UCSCi | uc008qca.1, mouse [Q8VHR5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF411837 mRNA Translation: AAL39081.1 AK041594 mRNA Translation: BAC30997.1 |
CCDSi | CCDS17526.1 [Q8VHR5-1] |
RefSeqi | NP_647465.1, NM_139304.1 [Q8VHR5-1] XP_006501422.2, XM_006501359.3 XP_006501424.1, XM_006501361.2 |
3D structure databases
AlphaFoldDBi | Q8VHR5 |
SMRi | Q8VHR5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230858, 18 interactors |
ComplexPortali | CPX-953, MBD2/NuRD nucleosome remodeling and deacetylase complex CPX-954, MBD3/NuRD nucleosome remodeling and deacetylase complex |
CORUMi | Q8VHR5 |
DIPi | DIP-62049N |
IntActi | Q8VHR5, 8 interactors |
MINTi | Q8VHR5 |
STRINGi | 10090.ENSMUSP00000041370 |
PTM databases
iPTMneti | Q8VHR5 |
PhosphoSitePlusi | Q8VHR5 |
Proteomic databases
EPDi | Q8VHR5 |
jPOSTi | Q8VHR5 |
MaxQBi | Q8VHR5 |
PaxDbi | Q8VHR5 |
PeptideAtlasi | Q8VHR5 |
PRIDEi | Q8VHR5 |
ProteomicsDBi | 294313 [Q8VHR5-1] 294314 [Q8VHR5-2] |
Protocols and materials databases
Antibodypediai | 1795, 272 antibodies from 30 providers |
DNASUi | 229542 |
Genome annotation databases
Ensembli | ENSMUST00000049382; ENSMUSP00000041370; ENSMUSG00000042390 [Q8VHR5-1] ENSMUST00000199607; ENSMUSP00000142617; ENSMUSG00000042390 [Q8VHR5-1] ENSMUST00000199754; ENSMUSP00000142514; ENSMUSG00000042390 [Q8VHR5-1] |
GeneIDi | 229542 |
KEGGi | mmu:229542 |
UCSCi | uc008qca.1, mouse [Q8VHR5-1] |
Organism-specific databases
CTDi | 57459 |
MGIi | MGI:2443225, Gatad2b |
VEuPathDBi | HostDB:ENSMUSG00000042390 |
Phylogenomic databases
eggNOGi | KOG3740, Eukaryota |
GeneTreei | ENSGT00390000004097 |
InParanoidi | Q8VHR5 |
OMAi | PEERMHQ |
OrthoDBi | 885815at2759 |
PhylomeDBi | Q8VHR5 |
TreeFami | TF321369 |
Enzyme and pathway databases
Reactomei | R-MMU-3214815, HDACs deacetylate histones R-MMU-6804758, Regulation of TP53 Activity through Acetylation R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-8943724, Regulation of PTEN gene transcription |
Miscellaneous databases
BioGRID-ORCSi | 229542, 4 hits in 63 CRISPR screens |
ChiTaRSi | Gatad2b, mouse |
PROi | PR:Q8VHR5 |
RNActi | Q8VHR5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042390, Expressed in secondary oocyte and 279 other tissues |
Genevisiblei | Q8VHR5, MM |
Family and domain databases
Gene3Di | 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR040386, P66 IPR032346, P66_CC IPR000679, Znf_GATA IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR13455, PTHR13455, 1 hit |
Pfami | View protein in Pfam PF00320, GATA, 1 hit PF16563, P66_CC, 1 hit |
PROSITEi | View protein in PROSITE PS50114, GATA_ZN_FINGER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | P66B_MOUSE | |
Accessioni | Q8VHR5Primary (citable) accession number: Q8VHR5 Secondary accession number(s): Q8C9Q3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot