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Protein

ATP-dependent RNA helicase DHX36

Gene

Dhx36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proposed to have a global role in regulating mRNA expression including transcriptional regulation and mRNA stability. Binds with high affinity to and resolves tetramolecular RNA and DNA quadruplex structures. Unwinds intramolecular quadruplexes derived from the ZIC1 and the MYC promoters. Binds to quadruplex structures in the promoters of YY1 and ALPL genes and regulates their expression. Binds to telomerase RNA template component (TERC) 5'-end (nucleotides 1-43) and unwinds an internal quadruplex formation in TERC 5'-end to promote P1 helix formation; the P1 helix acts as a template boundary ensuring accurate reverse transcription and is disrupted by quadruplex formation. May be involved in regulation of telomere length. Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. May play a role in ossification.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi223 – 230ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • ossification Source: UniProtKB
  • positive regulation of interferon-alpha secretion Source: MGI
  • positive regulation of telomere maintenance Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • response to exogenous dsRNA Source: MGI
  • response to virus Source: MGI
  • RNA processing Source: GO_Central
  • RNA secondary structure unwinding Source: UniProtKB
  • telomerase RNA stabilization Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase, RNA-binding
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3134963 DEx/H-box helicases activate type I IFN and inflammatory cytokines production
R-MMU-933541 TRAF6 mediated IRF7 activation
R-MMU-975110 TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX36 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 36
MLE-like protein 1
RNA helicase associated with AU-rich element ARE
Gene namesi
Name:Dhx36
Synonyms:Ddx36, Kiaa1488, Mlel1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1919412 Dhx36

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002475311 – 1001ATP-dependent RNA helicase DHX36Add BLAST1001

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei154PhosphoserineBy similarity1
Modified residuei940N6-acetyllysineBy similarity1
Modified residuei956PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VHK9
MaxQBiQ8VHK9
PaxDbiQ8VHK9
PeptideAtlasiQ8VHK9
PRIDEiQ8VHK9

PTM databases

iPTMnetiQ8VHK9
PhosphoSitePlusiQ8VHK9

Expressioni

Gene expression databases

BgeeiENSMUSG00000027770
CleanExiMM_DHX36
GenevisibleiQ8VHK9 MM

Interactioni

Subunit structurei

Interacts with AGO1, AGO2, PARN, EXOSC3, EXOSC10, HDAC1 and HDAC4.1 Publication

GO - Molecular functioni

  • histone deacetylase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi215193, 3 interactors
DIPiDIP-48576N
IntActiQ8VHK9, 3 interactors
STRINGi10090.ENSMUSP00000029336

Structurei

3D structure databases

ProteinModelPortaliQ8VHK9
SMRiQ8VHK9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini210 – 380Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini470 – 640Helicase C-terminalPROSITE-ProRule annotationAdd BLAST171

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 98RNA-binding; sufficient and required for recruitment to cytoplasmic stress granulesBy similarityAdd BLAST98
Regioni46 – 98Sufficient and required for interaction with TERC 5'-endBy similarityAdd BLAST53
Regioni47 – 59RSMAdd BLAST13

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili120 – 147Sequence analysisAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi327 – 330DEAH box4
Motifi510 – 521Nuclear localization signalBy similarityAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 44Gly-richAdd BLAST32

Domaini

The RHAU-specific motif (RSM) is required for quadruplex G4-DNA/RNA structure recognition and resolution.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0920 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00760000119189
HOGENOMiHOG000247063
HOVERGENiHBG081438
InParanoidiQ8VHK9
KOiK14442
OMAiTMVFPMA
OrthoDBiEOG091G01I0
TreeFamiTF324744

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VHK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYDYHQSWS RDGGPRGSGQ GSSGGGGGGS RGSGGGGGGR GGRGRHPAHL
60 70 80 90 100
KGREIGLWYA KKQTQKNKEA ERQERAVVHM DERREEQIVQ LLNSVQAKTD
110 120 130 140 150
KDSEAQISWF APEDHGYGTE VSSEKKINSE KKLDNQEKKL LNQEKKTFRI
160 170 180 190 200
TDKSYIDRDS EYLLQENEPN LSLDQHLLED LQRKKTDPRY IEMQRFRKKL
210 220 230 240 250
PSYGMQKELV NLINNHQVTV ISGETGCGKT TQVTQFILDN YIERGKGSAC
260 270 280 290 300
RIVCTQPRRI SAISVAERVA TERAESCGNG NSTGYQIRLQ SRLPRKQGSI
310 320 330 340 350
LYCTTGIILQ WLQSDSRLSS VSHIVLDEIH ERNLQSDVLM TVIKDLLHFR
360 370 380 390 400
SDLKVILMSA TLNAEKFSEY FGNCPMIHIP GFTFPVVEYL LEDIIEKIRY
410 420 430 440 450
VPDQKEHRSQ FKRGFMQGHV NRQEKEEKEA IYKERWPAYI KELRTRYSAS
460 470 480 490 500
TVDVLQMMDD DKVDLNLIAA LIRYIVLEEE DGAILVFLPG WDNISTLHDL
510 520 530 540 550
LMSQVMFKSD KFLIIPLHSL MPTVNQTQVF KKTPPGVRKI VIATNIAETS
560 570 580 590 600
ITIDDVVYVI DGGKIKETHF DTQNNISTMS AEWVSKANAK QRKGRAGRVQ
610 620 630 640 650
PGHCYHLYNG LRASLLDDYQ LPEILRTPLE ELCLQIKILR LGGIAYFLSR
660 670 680 690 700
LMDPPSNEAV VLSIKHLMEL SALDKQEELT PLGVHLARLP VEPHIGKMIL
710 720 730 740 750
FGALFCCLDP VLTIAASLSF KDPFVIPLGK EKIADARRKE LAKETRSDHL
760 770 780 790 800
TVVNAFEGWE EAKRRGFRYE KDYCWEYFLS SNTLQMLHNM KGQFAEHLLG
810 820 830 840 850
AGFVSSRSPK DPKANINSDN EKIIKAVICA GLYPKVAKIR LNLGKKRKMV
860 870 880 890 900
KVHTKSDGLV SIHPKSVNVE QTDFHYNWLI YHLKMRTSSI YLYDCTEVSP
910 920 930 940 950
YCLLFFGGDI SIQKDKDQEI IAVDEWIVFQ SPERIAHLVK GLRKELDSLL
960 970 980 990 1000
QEKIESPHPV DWDDTKSRDC AVLSAILDLI KTQEKATPRN LPPRSQDGYY

S
Length:1,001
Mass (Da):113,883
Last modified:October 3, 2012 - v2
Checksum:i4DD9292011B8155A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29Missing in BAC98183 (PubMed:14621295).Curated1
Sequence conflicti160S → T in BAC98183 (PubMed:14621295).Curated1
Sequence conflicti984E → K in AAL47006 (PubMed:12198572).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448804 mRNA Translation: AAL47006.1
AC114424 Genomic DNA No translation available.
CH466530 Genomic DNA Translation: EDL35384.1
AK129373 mRNA Translation: BAC98183.1
AK013031 mRNA Translation: BAB28610.1
CCDSiCCDS17379.1
RefSeqiNP_082412.2, NM_028136.2
XP_011238540.1, XM_011240238.1
UniGeneiMm.224233

Genome annotation databases

EnsembliENSMUST00000029336; ENSMUSP00000029336; ENSMUSG00000027770
GeneIDi72162
KEGGimmu:72162
UCSCiuc008pjn.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDHX36_MOUSE
AccessioniPrimary (citable) accession number: Q8VHK9
Secondary accession number(s): G3X8Y4, Q6ZPP7, Q9CSE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: October 3, 2012
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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