UniProtKB - Q8VHF0 (MARK3_RAT)
MAP/microtubule affinity-regulating kinase 3
Mark3
Functioni
Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAPT/TAU, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.
By similarityCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 85 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 178 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 62 – 70 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- tau-protein kinase activity Source: UniProtKB
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- microtubule cytoskeleton organization Source: GO_Central
- negative regulation of hippo signaling Source: UniProtKB
- peptidyl-serine autophosphorylation Source: ARUK-UCL
- peptidyl-serine phosphorylation Source: ARUK-UCL
- positive regulation of protein binding Source: ARUK-UCL
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-5673000, RAF activation R-RNO-5674135, MAP2K and MAPK activation R-RNO-5675221, Negative regulation of MAPK pathway |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Mark3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619883, Mark3 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- dendrite By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086306 | 1 – 797 | MAP/microtubule affinity-regulating kinase 3Add BLAST | 797 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 42 | PhosphoserineBy similarity | 1 | |
Modified residuei | 211 | Phosphothreonine; by LKB1By similarity | 1 | |
Modified residuei | 368 | PhosphoserineBy similarity | 1 | |
Modified residuei | 374 | PhosphoserineCombined sources | 1 | |
Modified residuei | 376 | PhosphoserineBy similarity | 1 | |
Modified residuei | 380 | PhosphoserineBy similarity | 1 | |
Modified residuei | 383 | PhosphoserineBy similarity | 1 | |
Modified residuei | 400 | PhosphoserineBy similarity | 1 | |
Modified residuei | 419 | PhosphoserineBy similarity | 1 | |
Modified residuei | 469 | PhosphoserineCombined sources | 1 | |
Modified residuei | 593 | PhosphoserineBy similarity | 1 | |
Modified residuei | 596 | PhosphoserineBy similarity | 1 | |
Modified residuei | 602 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 617 | Phosphothreonine; by PKC/PRKCZBy similarity | 1 | |
Modified residuei | 636 | PhosphoserineBy similarity | 1 | |
Modified residuei | 651 | PhosphoserineBy similarity | 1 | |
Modified residuei | 654 | PhosphoserineBy similarity | 1 | |
Modified residuei | 687 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8VHF0 |
PRIDEi | Q8VHF0 |
PTM databases
iPTMneti | Q8VHF0 |
PhosphoSitePlusi | Q8VHF0 |
Interactioni
Subunit structurei
Interacts with MAPT/TAU.
Interacts with DLG5 (via coiled-coil domain).
Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5.
Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 250933, 1 interactor |
IntActi | Q8VHF0, 2 interactors |
STRINGi | 10116.ENSRNOP00000014395 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 307 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 252 | |
Domaini | 326 – 365 | UBAPROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 748 – 797 | KA1PROSITE-ProRule annotationAdd BLAST | 50 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 35 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 372 – 504 | DisorderedSequence analysisAdd BLAST | 133 | |
Regioni | 585 – 701 | DisorderedSequence analysisAdd BLAST | 117 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 28 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 372 – 418 | Polar residuesSequence analysisAdd BLAST | 47 | |
Compositional biasi | 585 – 605 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 631 – 666 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 667 – 701 | Basic and acidic residuesSequence analysisAdd BLAST | 35 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0586, Eukaryota |
InParanoidi | Q8VHF0 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q8VHF0 |
Family and domain databases
InterProi | View protein in InterPro IPR028375, KA1/Ssp2_C IPR001772, KA1_dom IPR011009, Kinase-like_dom_sf IPR033628, MARK3 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR015940, UBA |
PANTHERi | PTHR24346:SF1, PTHR24346:SF1, 1 hit |
Pfami | View protein in Pfam PF02149, KA1, 1 hit PF00069, Pkinase, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit SM00165, UBA, 1 hit |
SUPFAMi | SSF103243, SSF103243, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50032, KA1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50030, UBA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSTRTPLPTV NERDTENHIS HGDGRQEVTS RTGRSGARCR NSIASCADEQ
60 70 80 90 100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL
110 120 130 140 150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM
160 170 180 190 200
KEKEARAKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKITDFGFS
210 220 230 240 250
NEFTVGSKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS
260 270 280 290 300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPVKRGTLEQ
310 320 330 340 350
IMKDRWINAG HEEEELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS
360 370 380 390 400
KMKYDEITAT YLLLGRKSAE LDASDSSSSS NLSLAKVRPS SDLSNSTGQS
410 420 430 440 450
PHHKGQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGV
460 470 480 490 500
PSRKSGSSAV GGKGIAPASP MLGNASNPNK ADIPERKKSP AVPSSNAASG
510 520 530 540 550
GMTRRNTYVC SERCAADRHS VIQNGKESSL TEVFAYAASP ASLCATSTCR
560 570 580 590 600
LRHQRSMSVS ASGHPKMVLP PIDSEGDTFK AITTPDQRTP VASTHSISSA
610 620 630 640 650
TTPDRIRFPR GTASRSTFHG QPRERRTATY NGPPASPSLS HEATPLSQTR
660 670 680 690 700
SRGSTNLFSK LTSKLTRRLP TEYERNGRYE GSSRNVSSEQ KDENREAKPR
710 720 730 740 750
SLRFTWSMKT TSSMDPSDMM REIRKVLDAN TCDYEQRERF LLFCVHGDGH
760 770 780 790
AESLVQWEME VCKLPRLSLN GVRFKRISGT SIAFKNIASK IANELKL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JU56 | A0A0G2JU56_RAT | Non-specific serine/threonine prote... | Mark3 | 738 | Annotation score: | ||
F1M836 | F1M836_RAT | Non-specific serine/threonine prote... | Mark3 | 798 | Annotation score: | ||
A0A0G2K554 | A0A0G2K554_RAT | MAP/microtubule affinity-regulating... | Mark3 | 797 | Annotation score: | ||
A0A1B0GWN5 | A0A1B0GWN5_RAT | Non-specific serine/threonine prote... | Mark3 | 759 | Annotation score: | ||
A0A8I5ZV33 | A0A8I5ZV33_RAT | MAP/microtubule affinity-regulating... | Mark3 | 744 | Annotation score: | ||
A0A8I6GIW6 | A0A8I6GIW6_RAT | MAP/microtubule affinity-regulating... | Mark3 | 729 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF465412 mRNA Translation: AAL69981.1 |
RefSeqi | NP_570105.1, NM_130749.1 |
Genome annotation databases
GeneIDi | 170577 |
KEGGi | rno:170577 |
UCSCi | RGD:619883, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF465412 mRNA Translation: AAL69981.1 |
RefSeqi | NP_570105.1, NM_130749.1 |
3D structure databases
AlphaFoldDBi | Q8VHF0 |
SMRi | Q8VHF0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250933, 1 interactor |
IntActi | Q8VHF0, 2 interactors |
STRINGi | 10116.ENSRNOP00000014395 |
PTM databases
iPTMneti | Q8VHF0 |
PhosphoSitePlusi | Q8VHF0 |
Proteomic databases
PaxDbi | Q8VHF0 |
PRIDEi | Q8VHF0 |
Genome annotation databases
GeneIDi | 170577 |
KEGGi | rno:170577 |
UCSCi | RGD:619883, rat |
Organism-specific databases
CTDi | 4140 |
RGDi | 619883, Mark3 |
Phylogenomic databases
eggNOGi | KOG0586, Eukaryota |
InParanoidi | Q8VHF0 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q8VHF0 |
Enzyme and pathway databases
Reactomei | R-RNO-5673000, RAF activation R-RNO-5674135, MAP2K and MAPK activation R-RNO-5675221, Negative regulation of MAPK pathway |
Miscellaneous databases
PROi | PR:Q8VHF0 |
Family and domain databases
InterProi | View protein in InterPro IPR028375, KA1/Ssp2_C IPR001772, KA1_dom IPR011009, Kinase-like_dom_sf IPR033628, MARK3 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR015940, UBA |
PANTHERi | PTHR24346:SF1, PTHR24346:SF1, 1 hit |
Pfami | View protein in Pfam PF02149, KA1, 1 hit PF00069, Pkinase, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit SM00165, UBA, 1 hit |
SUPFAMi | SSF103243, SSF103243, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50032, KA1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50030, UBA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MARK3_RAT | |
Accessioni | Q8VHF0Primary (citable) accession number: Q8VHF0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families