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Protein

Translocation protein SEC63 homolog

Gene

Sec63

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER) (PubMed:22375059). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen (By similarity). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (PubMed:21685914).By similarity2 Publications

GO - Molecular functioni

  • protein transporter activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-381038 XBP1(S) activates chaperone genes

Names & Taxonomyi

Protein namesi
Recommended name:
Translocation protein SEC63 homolog
Gene namesi
Name:Sec63
Synonyms:Sec63l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2155302 Sec63

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14LumenalSequence analysisAdd BLAST14
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Topological domaini36 – 69CytoplasmicSequence analysisAdd BLAST34
Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
Topological domaini91 – 188LumenalSequence analysisAdd BLAST98
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 760CytoplasmicSequence analysisAdd BLAST551

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Knockout mice exhibit very early embryonic lethality before E7.5. Conditional ubiquitous or kidney-specidic knockdown results in polycystic liver and kidney phenotypes, respectively.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710981 – 760Translocation protein SEC63 homologAdd BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei537PhosphothreonineBy similarity1
Modified residuei742PhosphoserineBy similarity1
Modified residuei748PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VHE0
MaxQBiQ8VHE0
PaxDbiQ8VHE0
PeptideAtlasiQ8VHE0
PRIDEiQ8VHE0

PTM databases

iPTMnetiQ8VHE0
PhosphoSitePlusiQ8VHE0
SwissPalmiQ8VHE0

Expressioni

Tissue specificityi

Expressed in kidney (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000019802 Expressed in 283 organ(s), highest expression level in seminal vesicle
CleanExiMM_SEC63
GenevisibleiQ8VHE0 MM

Interactioni

Subunit structurei

The ER translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63.By similarity

Protein-protein interaction databases

BioGridi228308, 1 interactor
IntActiQ8VHE0, 2 interactors
MINTiQ8VHE0
STRINGi10090.ENSMUSP00000019937

Structurei

3D structure databases

ProteinModelPortaliQ8VHE0
SMRiQ8VHE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 165JPROSITE-ProRule annotationAdd BLAST62
Domaini367 – 457SEC63 1Add BLAST91
Domaini637 – 714SEC63 2Add BLAST78

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili603 – 635Sequence analysisAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi734 – 760Asp/Glu-rich (highly acidic)Add BLAST27

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0721 Eukaryota
KOG4434 Eukaryota
COG5407 LUCA
GeneTreeiENSGT00390000001965
HOGENOMiHOG000037933
HOVERGENiHBG057075
InParanoidiQ8VHE0
KOiK09540
OMAiSQFDYEM
OrthoDBiEOG091G03YI
TreeFamiTF105904

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR035892 C2_domain_sf
IPR001623 DnaJ_domain
IPR014756 Ig_E-set
IPR036869 J_dom_sf
IPR027137 Sec63
IPR004179 Sec63-dom
PANTHERiPTHR24075:SF0 PTHR24075:SF0, 1 hit
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF02889 Sec63, 2 hits
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM00973 Sec63, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VHE0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGQQFQYDD SGNTFFYFLT SFVGLIVIPA TYYLWPRDQN AEQIRLKNIR
60 70 80 90 100
KVYGRCMWYR LRLLKPQPNI IPTVKKIVLL AGWALFLFLA YKVSKTDREY
110 120 130 140 150
QEYNPYEVLN LDPGATVAEI KKQYRLLSLK YHPDKGGDEV MFMRIAKAYA
160 170 180 190 200
ALTDEESRKN WEEFGNPDGP QATSFGIALP AWIVDQKNSI LVLLVYGLAF
210 220 230 240 250
MVILPVVVGS WWYRSIRYSG DQILIRTTQI YTYFVYKTRN MDMKRLIMVL
260 270 280 290 300
AGASEFDPQY NKDSTSRPTD NILIPQLIRE IGSINLKKNE PPLTCPYSLK
310 320 330 340 350
ARVLLLSHLA RMKIPETLEE DQQFMLKKCP ALLQEMVNVI CQLIIMARSR
360 370 380 390 400
EEREFRAPTL ASLENCMKLS QMAVQGLQQF KSPLLQLPHI EEDNLRRVSN
410 420 430 440 450
HKKYKIKTIQ DLVSLKESDR HSLLHFLEDE KYEEVMAVLG SFPYVTMDIK
460 470 480 490 500
SQVLDDEDSN NITVGSLVTV LVKLTRQTMA EVFEKEQSIC AAEEQPTEDG
510 520 530 540 550
QSDANKIKAK GGWQQKNKGP KKMPKSKKKK PLKKKPTTVP LPQAKQQKQK
560 570 580 590 600
QANGVVGSEA AIKEEEDDIS DKGSDSEEEE TNRDSQSEKE DGSDRESDRE
610 620 630 640 650
QDEKQSKDDE AEWQELQQSI QRKERALLET KSKITHPVYS LYFPEEKQEW
660 670 680 690 700
WWLYIADRKE QTLISMPYHV CTLKDTEEVE LKFPAPGKPG NYQYTVFLRS
710 720 730 740 750
DSYMGLDQIK PLKLEVHEAK PVPENHPQWD TAIEGDEDQE DSEGFEDSFE
760
EEEEEEEGGD
Length:760
Mass (Da):87,870
Last modified:July 27, 2011 - v4
Checksum:i5966EDEE6F767384
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48N → S in AAK00580 (Ref. 1) Curated1
Sequence conflicti267R → K in AAH19366 (PubMed:15489334).Curated1
Sequence conflicti267R → K in AAH31846 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024346 mRNA Translation: AAK00580.1
AC124998 Genomic DNA No translation available.
BC019366 mRNA Translation: AAH19366.1
BC031846 mRNA Translation: AAH31846.1
CCDSiCCDS23815.1
RefSeqiNP_694695.3, NM_153055.3
UniGeneiMm.214344

Genome annotation databases

EnsembliENSMUST00000019937; ENSMUSP00000019937; ENSMUSG00000019802
GeneIDi140740
KEGGimmu:140740
UCSCiuc007eyx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024346 mRNA Translation: AAK00580.1
AC124998 Genomic DNA No translation available.
BC019366 mRNA Translation: AAH19366.1
BC031846 mRNA Translation: AAH31846.1
CCDSiCCDS23815.1
RefSeqiNP_694695.3, NM_153055.3
UniGeneiMm.214344

3D structure databases

ProteinModelPortaliQ8VHE0
SMRiQ8VHE0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228308, 1 interactor
IntActiQ8VHE0, 2 interactors
MINTiQ8VHE0
STRINGi10090.ENSMUSP00000019937

PTM databases

iPTMnetiQ8VHE0
PhosphoSitePlusiQ8VHE0
SwissPalmiQ8VHE0

Proteomic databases

EPDiQ8VHE0
MaxQBiQ8VHE0
PaxDbiQ8VHE0
PeptideAtlasiQ8VHE0
PRIDEiQ8VHE0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019937; ENSMUSP00000019937; ENSMUSG00000019802
GeneIDi140740
KEGGimmu:140740
UCSCiuc007eyx.2 mouse

Organism-specific databases

CTDi11231
MGIiMGI:2155302 Sec63

Phylogenomic databases

eggNOGiKOG0721 Eukaryota
KOG4434 Eukaryota
COG5407 LUCA
GeneTreeiENSGT00390000001965
HOGENOMiHOG000037933
HOVERGENiHBG057075
InParanoidiQ8VHE0
KOiK09540
OMAiSQFDYEM
OrthoDBiEOG091G03YI
TreeFamiTF105904

Enzyme and pathway databases

ReactomeiR-MMU-381038 XBP1(S) activates chaperone genes

Miscellaneous databases

ChiTaRSiSec63 mouse
PROiPR:Q8VHE0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019802 Expressed in 283 organ(s), highest expression level in seminal vesicle
CleanExiMM_SEC63
GenevisibleiQ8VHE0 MM

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR035892 C2_domain_sf
IPR001623 DnaJ_domain
IPR014756 Ig_E-set
IPR036869 J_dom_sf
IPR027137 Sec63
IPR004179 Sec63-dom
PANTHERiPTHR24075:SF0 PTHR24075:SF0, 1 hit
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF02889 Sec63, 2 hits
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM00973 Sec63, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSEC63_MOUSE
AccessioniPrimary (citable) accession number: Q8VHE0
Secondary accession number(s): E9QKG1, Q8VEB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 138 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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