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Protein

Histone deacetylase 8

Gene

Hdac8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility (By similarity).By similarity

Catalytic activityi

Hydrolysis of an N6-acetyl-lysine residue of a histone to yield a deacetylated histone.

Cofactori

a divalent metal cationNote: Binds 1 divalent metal cation per subunit.

Activity regulationi

Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors. histone deacetylase inhibitor (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101SubstrateBy similarity1
Active sitei143Proton acceptorBy similarity1
Binding sitei151Substrate; via carbonyl oxygenBy similarity1
Metal bindingi178Divalent metal cationBy similarity1
Metal bindingi180Divalent metal cationBy similarity1
Metal bindingi267Divalent metal cationBy similarity1
Binding sitei306SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Hydrolase, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-3214815 HDACs deacetylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase 8 (EC:3.5.1.98)
Short name:
HD8
Gene namesi
Name:Hdac8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1917565 Hdac8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2347

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147091 – 377Histone deacetylase 8Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VH37
PaxDbiQ8VH37
PeptideAtlasiQ8VH37
PRIDEiQ8VH37

PTM databases

PhosphoSitePlusiQ8VH37

Expressioni

Gene expression databases

BgeeiENSMUSG00000067567 Expressed in 185 organ(s), highest expression level in epithelium of esophagus
CleanExiMM_HDAC8
ExpressionAtlasiQ8VH37 baseline and differential
GenevisibleiQ8VH37 MM

Interactioni

Subunit structurei

Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213982, 1 interactor
IntActiQ8VH37, 1 interactor
STRINGi10090.ENSMUSP00000085226

Chemistry databases

BindingDBiQ8VH37

Structurei

3D structure databases

ProteinModelPortaliQ8VH37
SMRiQ8VH37
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 324Histone deacetylaseAdd BLAST311

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1342 Eukaryota
COG0123 LUCA
GeneTreeiENSGT00910000144047
HOGENOMiHOG000225180
HOVERGENiHBG057112
InParanoidiQ8VH37
KOiK11405
OMAiCGYDANA
OrthoDBiEOG091G0A9R
PhylomeDBiQ8VH37
TreeFamiTF106175

Family and domain databases

Gene3Di3.40.800.20, 1 hit
InterProiView protein in InterPro
IPR000286 His_deacetylse
IPR003084 His_deacetylse_1
IPR023801 His_deacetylse_dom
IPR037138 His_deacetylse_dom_sf
IPR023696 Ureohydrolase_dom_sf
PANTHERiPTHR10625 PTHR10625, 1 hit
PfamiView protein in Pfam
PF00850 Hist_deacetyl, 1 hit
PIRSFiPIRSF037913 His_deacetylse_1, 1 hit
PRINTSiPR01270 HDASUPER
PR01271 HISDACETLASE
SUPFAMiSSF52768 SSF52768, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8VH37-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEMPEEPANS GHSLPPVYIY SPEYVSICDS LVKVPKRASM VHSLIEAYAL
60 70 80 90 100
HKQMRIVKPK VASMEEMATF HTDAYLQHLQ KVSQEGDEDH PDSIEYGLGY
110 120 130 140 150
DCPATEGIFD YAAAIGGGTI TAAQCLIDGK CKVAINWSGG WHHAKKDEAS
160 170 180 190 200
GFCYLNDAVL GILRLRRKFD RILYVDLDLH HGDGVEDAFS FTSKVMTVSL
210 220 230 240 250
HKFSPGFFPG TGDMSDVGLG KGRYYSVNVP IQDGIQDEKY YHICESVLKE
260 270 280 290 300
VYQAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY VLQWQLATLI
310 320 330 340 350
LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP
360 370
SCRPDRNEPH RIQQILNYIK GNLKHVV
Length:377
Mass (Da):41,772
Last modified:April 11, 2003 - v2
Checksum:iEEBD7B2517B235CD
GO
Isoform 2 (identifier: Q8VH37-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-253: SVLKEVYQ → RACFTRTP
     254-377: Missing.

Show »
Length:253
Mass (Da):28,122
Checksum:i982E94F21829B340
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R2V6S4R2V6_MOUSE
Histone deacetylase 8
Hdac8
56Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88E → D in AAL47569 (Ref. 3) Curated1
Sequence conflicti118G → A in AAL47569 (Ref. 3) Curated1
Sequence conflicti130K → M in AAL47569 (Ref. 3) Curated1
Sequence conflicti170D → E in AAL47569 (Ref. 3) Curated1
Sequence conflicti187D → V in AAL47569 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007178246 – 253SVLKEVYQ → RACFTRTP in isoform 2. 1 Publication8
Alternative sequenceiVSP_007179254 – 377Missing in isoform 2. 1 PublicationAdd BLAST124

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011332 mRNA Translation: BAB27550.1
AK131998 mRNA Translation: BAE20928.1
BC061257 mRNA Translation: AAH61257.1
AK034511 mRNA Translation: BAC28737.1
AK041965 mRNA Translation: BAC31116.1
AY066003 mRNA Translation: AAL47569.1
CCDSiCCDS41084.1 [Q8VH37-1]
RefSeqiNP_001300671.1, NM_001313742.1 [Q8VH37-2]
NP_081658.1, NM_027382.4 [Q8VH37-1]
UniGeneiMm.328128

Genome annotation databases

EnsembliENSMUST00000087916; ENSMUSP00000085226; ENSMUSG00000067567 [Q8VH37-1]
GeneIDi70315
KEGGimmu:70315
UCSCiuc009typ.1 mouse [Q8VH37-1]
uc009tyq.1 mouse [Q8VH37-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011332 mRNA Translation: BAB27550.1
AK131998 mRNA Translation: BAE20928.1
BC061257 mRNA Translation: AAH61257.1
AK034511 mRNA Translation: BAC28737.1
AK041965 mRNA Translation: BAC31116.1
AY066003 mRNA Translation: AAL47569.1
CCDSiCCDS41084.1 [Q8VH37-1]
RefSeqiNP_001300671.1, NM_001313742.1 [Q8VH37-2]
NP_081658.1, NM_027382.4 [Q8VH37-1]
UniGeneiMm.328128

3D structure databases

ProteinModelPortaliQ8VH37
SMRiQ8VH37
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213982, 1 interactor
IntActiQ8VH37, 1 interactor
STRINGi10090.ENSMUSP00000085226

Chemistry databases

BindingDBiQ8VH37
ChEMBLiCHEMBL2347

PTM databases

PhosphoSitePlusiQ8VH37

Proteomic databases

EPDiQ8VH37
PaxDbiQ8VH37
PeptideAtlasiQ8VH37
PRIDEiQ8VH37

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087916; ENSMUSP00000085226; ENSMUSG00000067567 [Q8VH37-1]
GeneIDi70315
KEGGimmu:70315
UCSCiuc009typ.1 mouse [Q8VH37-1]
uc009tyq.1 mouse [Q8VH37-2]

Organism-specific databases

CTDi55869
MGIiMGI:1917565 Hdac8

Phylogenomic databases

eggNOGiKOG1342 Eukaryota
COG0123 LUCA
GeneTreeiENSGT00910000144047
HOGENOMiHOG000225180
HOVERGENiHBG057112
InParanoidiQ8VH37
KOiK11405
OMAiCGYDANA
OrthoDBiEOG091G0A9R
PhylomeDBiQ8VH37
TreeFamiTF106175

Enzyme and pathway databases

ReactomeiR-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-3214815 HDACs deacetylate histones

Miscellaneous databases

PROiPR:Q8VH37
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067567 Expressed in 185 organ(s), highest expression level in epithelium of esophagus
CleanExiMM_HDAC8
ExpressionAtlasiQ8VH37 baseline and differential
GenevisibleiQ8VH37 MM

Family and domain databases

Gene3Di3.40.800.20, 1 hit
InterProiView protein in InterPro
IPR000286 His_deacetylse
IPR003084 His_deacetylse_1
IPR023801 His_deacetylse_dom
IPR037138 His_deacetylse_dom_sf
IPR023696 Ureohydrolase_dom_sf
PANTHERiPTHR10625 PTHR10625, 1 hit
PfamiView protein in Pfam
PF00850 Hist_deacetyl, 1 hit
PIRSFiPIRSF037913 His_deacetylse_1, 1 hit
PRINTSiPR01270 HDASUPER
PR01271 HISDACETLASE
SUPFAMiSSF52768 SSF52768, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHDAC8_MOUSE
AccessioniPrimary (citable) accession number: Q8VH37
Secondary accession number(s): Q3V270, Q9D0K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: April 11, 2003
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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