UniProtKB - Q8VGC3 (CACB2_RAT)
Voltage-dependent L-type calcium channel subunit beta-2
Cacnb2
Functioni
The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
By similarity3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 544 | Required for CaMK2D-binding | 1 |
GO - Molecular functioni
- actin filament binding Source: BHF-UCL
- calcium channel regulator activity Source: RGD
- high voltage-gated calcium channel activity Source: GO_Central
- identical protein binding Source: IntAct
- phosphoprotein binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase binding Source: RGD
- voltage-gated calcium channel activity Source: RGD
- voltage-gated calcium channel activity involved in positive regulation of presynaptic cytosolic calcium levels Source: RGD
GO - Biological processi
- calcium ion import Source: RGD
- calcium ion transport Source: RGD
- chemical synaptic transmission Source: RGD
- membrane depolarization during atrial cardiac muscle cell action potential Source: BHF-UCL
- membrane depolarization during AV node cell action potential Source: BHF-UCL
- neuromuscular junction development Source: GO_Central
- positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel Source: BHF-UCL
- positive regulation of calcium ion transport Source: RGD
- positive regulation of high voltage-gated calcium channel activity Source: BHF-UCL
- protein localization to plasma membrane Source: BHF-UCL
- regulation of heart rate by cardiac conduction Source: BHF-UCL
- regulation of voltage-gated calcium channel activity Source: RGD
- visual perception Source: RGD
Keywordsi
Molecular function | Calcium channel, Ion channel, Voltage-gated channel |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium |
Enzyme and pathway databases
BRENDAi | 2.7.4.8, 5301 |
Reactomei | R-RNO-112308, Presynaptic depolarization and calcium channel opening R-RNO-422356, Regulation of insulin secretion R-RNO-5576892, Phase 0 - rapid depolarisation R-RNO-5576893, Phase 2 - plateau phase |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent L-type calcium channel subunit beta-2Short name: CAB2 Alternative name(s): Calcium channel voltage-dependent subunit beta 2 |
Gene namesi | Name:Cacnb2 Synonyms:Cacnlb2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 67385, Cacnb2 |
Subcellular locationi
Plasma membrane
- sarcolemma 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Plasma Membrane
- L-type voltage-gated calcium channel complex Source: UniProtKB
- voltage-gated calcium channel complex Source: RGD
Other locations
- photoreceptor ribbon synapse Source: RGD
- presynapse Source: GOC
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144054 | 1 – 655 | Voltage-dependent L-type calcium channel subunit beta-2Add BLAST | 655 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 200 | PhosphoserineCombined sources | 1 | |
Modified residuei | 203 | PhosphoserineCombined sources | 1 | |
Modified residuei | 214 | PhosphoserineBy similarity | 1 | |
Modified residuei | 545 | PhosphoserineBy similarity | 1 | |
Modified residuei | 549 | Phosphothreonine; by CaMK2D1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8VGC3 |
PRIDEi | Q8VGC3 |
PTM databases
CarbonylDBi | Q8VGC3 |
iPTMneti | Q8VGC3 |
PhosphoSitePlusi | Q8VGC3 |
SwissPalmi | Q8VGC3 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1S) and the ancillary subunits CACNB1 or CACNB2, CACNG1 and CACNA2D1 (PubMed:26680202). The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio, i.e. it contains either CACNB1 or CACNB2 (PubMed:26680202).
Interacts with CACNA1C (PubMed:15141227).
Interacts with RRAD. Interaction with RRAD regulates the trafficking of CACNA1C to the cell membrane (By similarity).
Interacts with TMIGD2 (By similarity).
Interacts with CAMK2D (PubMed:20194790).
Interacts with CBARP (PubMed:24751537).
Interacts with CAMK2A (By similarity).
By similarity4 PublicationsBinary interactionsi
Cacnb2 - isoform 2 [Q8VGC3-2]
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-15685453,EBI-15685453 |
GO - Molecular functioni
- actin filament binding Source: BHF-UCL
- identical protein binding Source: IntAct
- phosphoprotein binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase binding Source: RGD
Protein-protein interaction databases
DIPi | DIP-29590N |
IntActi | Q8VGC3, 5 interactors |
STRINGi | 10116.ENSRNOP00000037354 |
Structurei
Secondary structure
3D structure databases
SMRi | Q8VGC3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8VGC3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 110 – 179 | SH3PROSITE-ProRule annotationAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 107 | DisorderedSequence analysisAdd BLAST | 74 | |
Regioni | 186 – 257 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 483 – 632 | DisorderedSequence analysisAdd BLAST | 150 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 53 – 79 | Polar residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 80 – 103 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 190 – 221 | Polar residuesSequence analysisAdd BLAST | 32 | |
Compositional biasi | 240 – 254 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 483 – 506 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 523 – 548 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 549 – 595 | Basic and acidic residuesSequence analysisAdd BLAST | 47 | |
Compositional biasi | 603 – 632 | Basic and acidic residuesSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3812, Eukaryota |
InParanoidi | Q8VGC3 |
OMAi | TDREQDH |
OrthoDBi | 926074at2759 |
PhylomeDBi | Q8VGC3 |
Family and domain databases
CDDi | cd12040, SH3_CACNB2, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR035605, CACNB2_SH3 IPR008145, GK/Ca_channel_bsu IPR027417, P-loop_NTPase IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR005444, VDCC_L_b2su IPR000584, VDCC_L_bsu |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF12052, VGCC_beta4Aa_N, 1 hit |
PRINTSi | PR01626, LCACHANNELB PR01628, LCACHANNELB2 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVQSDTSKSP PIAAVAQESQ MELLESAAPA GALGAQSYGK GARRKNRFKG
60 70 80 90 100
SDDSTSSDTT SNSFVRQGSA DSYTSRPSDS DVSLEEDREA VRREAERQAQ
110 120 130 140 150
AQLEKAKTKP VAFAVRTNVR YSAAQEDDVP VPGMAISFEA KDFLHVKEKF
160 170 180 190 200
NNDWWIGRLV KEGCEIGFIP SPVKLENMRL QHEQRAKQGK FYSSKSGGNS
210 220 230 240 250
SSSLGDIVPS SRKSTPPSSA IDIDATGLDA EENDIPANHR SPKPSANSVT
260 270 280 290 300
SPHSKEKRMP FFKKTEHTPP YDVVPSMRPV VLVGPSLKGY EVTDMMQKAL
310 320 330 340 350
FDFLKHRFEG RISITRVTAD ISLAKRSVLN NPSKHAIIER SNTRSSLAEV
360 370 380 390 400
QSEIERIFEL ARTLQLVVLD ADTINHPAQL SKTSLAPIIV YVKISSPKVL
410 420 430 440 450
QRLIKSRGKS QAKHLNVQMV AADKLAQCPP QESFDVILDE NQLEDACEHL
460 470 480 490 500
ADYLEAYWKA THPPSSNLPN PLLSRTLATS TLPLSPTLAS NSQGSQGDQR
510 520 530 540 550
TDRSAPRSAS QAEEEPCLEP VKKSQHRSSS ATHQNHRSGT GRGLSRQETF
560 570 580 590 600
DSETQESRDS AYVEPKEDYS HEHVDRYVPH REHNHREESH SSNGHRHREP
610 620 630 640 650
RHRTRDMGRD QDHNECSKQR SRHKSKDRYC DKEGEVISKR RSEAGEWNRD
VYIRQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZWK0 | D3ZWK0_RAT | Calcium channel voltage-dependent s... | Cacnb2 rCG_55786 | 611 | Annotation score: | ||
A0A0G2JSZ0 | A0A0G2JSZ0_RAT | Calcium channel voltage-dependent s... | Cacnb2 rCG_55786 | 604 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 53 | D → G in AAO38996 (PubMed:12042350).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010734 | 1 – 67 | MVQSD…SFVRQ → MQCCGLVHRRRVRVSR in isoform 2. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_010735 | 1 – 67 | MVQSD…SFVRQ → MKATWIRLLKRAKGERLKDS DIC in isoform 4. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_010736 | 1 – 67 | MVQSD…SFVRQ → MLDRQLVSSQTQSSIPG in isoform 5. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_010733 | 1 – 36 | MVQSD…ALGAQ → MDQASGLDRLKI in isoform 3. 1 PublicationAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF394941 mRNA Translation: AAL47074.1 M80545 mRNA Translation: AAK14821.1 AF423193 mRNA Translation: AAL16952.1 AY190119 mRNA Translation: AAO38996.1 AY190120 mRNA Translation: AAO38997.1 |
PIRi | A42044 |
RefSeqi | NP_446303.1, NM_053851.1 XP_006254360.1, XM_006254298.3 XP_006254365.1, XM_006254303.3 [Q8VGC3-5] |
Genome annotation databases
GeneIDi | 116600 |
KEGGi | rno:116600 |
UCSCi | RGD:67385, rat [Q8VGC3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF394941 mRNA Translation: AAL47074.1 M80545 mRNA Translation: AAK14821.1 AF423193 mRNA Translation: AAL16952.1 AY190119 mRNA Translation: AAO38996.1 AY190120 mRNA Translation: AAO38997.1 |
PIRi | A42044 |
RefSeqi | NP_446303.1, NM_053851.1 XP_006254360.1, XM_006254298.3 XP_006254365.1, XM_006254303.3 [Q8VGC3-5] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T0H | X-ray | 1.97 | A | 68-196 | [»] | |
B | 254-476 | [»] | ||||
1T0J | X-ray | 2.00 | A | 68-196 | [»] | |
B | 254-476 | [»] | ||||
3JBR | electron microscopy | 4.20 | B | 68-196 | [»] | |
B | 254-475 | [»] | ||||
5V2P | X-ray | 2.00 | A | 68-189 | [»] | |
A | 254-476 | [»] | ||||
5V2Q | X-ray | 1.70 | A | 68-189 | [»] | |
A | 254-476 | [»] | ||||
SMRi | Q8VGC3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29590N |
IntActi | Q8VGC3, 5 interactors |
STRINGi | 10116.ENSRNOP00000037354 |
PTM databases
CarbonylDBi | Q8VGC3 |
iPTMneti | Q8VGC3 |
PhosphoSitePlusi | Q8VGC3 |
SwissPalmi | Q8VGC3 |
Proteomic databases
PaxDbi | Q8VGC3 |
PRIDEi | Q8VGC3 |
Protocols and materials databases
ABCDi | Q8VGC3, 1 sequenced antibody |
Genome annotation databases
GeneIDi | 116600 |
KEGGi | rno:116600 |
UCSCi | RGD:67385, rat [Q8VGC3-1] |
Organism-specific databases
CTDi | 783 |
RGDi | 67385, Cacnb2 |
Phylogenomic databases
eggNOGi | KOG3812, Eukaryota |
InParanoidi | Q8VGC3 |
OMAi | TDREQDH |
OrthoDBi | 926074at2759 |
PhylomeDBi | Q8VGC3 |
Enzyme and pathway databases
BRENDAi | 2.7.4.8, 5301 |
Reactomei | R-RNO-112308, Presynaptic depolarization and calcium channel opening R-RNO-422356, Regulation of insulin secretion R-RNO-5576892, Phase 0 - rapid depolarisation R-RNO-5576893, Phase 2 - plateau phase |
Miscellaneous databases
EvolutionaryTracei | Q8VGC3 |
PROi | PR:Q8VGC3 |
Family and domain databases
CDDi | cd12040, SH3_CACNB2, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR035605, CACNB2_SH3 IPR008145, GK/Ca_channel_bsu IPR027417, P-loop_NTPase IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR005444, VDCC_L_b2su IPR000584, VDCC_L_bsu |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF12052, VGCC_beta4Aa_N, 1 hit |
PRINTSi | PR01626, LCACHANNELB PR01628, LCACHANNELB2 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CACB2_RAT | |
Accessioni | Q8VGC3Primary (citable) accession number: Q8VGC3 Secondary accession number(s): Q811Q6 Q9QUU7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families