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Protein

Notchless protein homolog 1

Gene

Nle1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in regulating Notch activity (Probable). Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival.Curated2 Publications

GO - Biological processi

Keywordsi

Biological processNotch signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Notchless protein homolog 1
Gene namesi
Name:Nle1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2429770 Nle1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality prior to E6.5 with death of inner mass cells. Embryos implant but die shortly after.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi163S → P in l11Jus4; embryos survive through implantation but fail prior to gastrulation. 1 Publication1
Mutagenesisi396I → S in l11Jus1; embryos survive through implantation but fail prior to gastrulation. Cdkna1 expression is up-regulated while expression of several Wnt pathway members is down-regulated. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000510992 – 485Notchless protein homolog 1Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei79PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VEJ4
MaxQBiQ8VEJ4
PaxDbiQ8VEJ4
PeptideAtlasiQ8VEJ4
PRIDEiQ8VEJ4

PTM databases

iPTMnetiQ8VEJ4
PhosphoSitePlusiQ8VEJ4

Expressioni

Tissue specificityi

Highest expression in heart, brain, lung, liver, skeletal muscle and testis with lower levels in spleen and kidney.1 Publication

Developmental stagei

Expression detected in the embryo at E7.0, E11.0 and E15.0 and also in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000020692 Expressed in 160 organ(s), highest expression level in bone marrow
CleanExiMM_NLE1
ExpressionAtlasiQ8VEJ4 baseline and differential
GenevisibleiQ8VEJ4 MM

Interactioni

Protein-protein interaction databases

BioGridi229831, 1 interactor
IntActiQ8VEJ4, 1 interactor
MINTiQ8VEJ4
STRINGi10090.ENSMUSP00000099502

Structurei

3D structure databases

ProteinModelPortaliQ8VEJ4
SMRiQ8VEJ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati112 – 151WD 1Add BLAST40
Repeati154 – 193WD 2Add BLAST40
Repeati197 – 241WD 3Add BLAST45
Repeati244 – 282WD 4Add BLAST39
Repeati327 – 366WD 5Add BLAST40
Repeati370 – 409WD 6Add BLAST40
Repeati412 – 451WD 7Add BLAST40
Repeati454 – 484WD 8Add BLAST31

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0271 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091641
HOVERGENiHBG007602
InParanoidiQ8VEJ4
KOiK14855
OMAiLNTETPH
OrthoDBiEOG091G05DP
TreeFamiTF300668

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR012972 NLE
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF08154 NLE, 1 hit
PF00400 WD40, 7 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 8 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 7 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8VEJ4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAVVEEAA AGDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDKLQLVCNA
60 70 80 90 100
LLAQEEPLPL AFYVHDAEIV SSLGKTLESQ SVETEKIVDI IYQPQAVFRV
110 120 130 140 150
RAVTRCTSSL EGHSEAVISV AFSPTGKYLA SGSGDTTVRF WDLSTETPHF
160 170 180 190 200
TCKGHRHWVL SISWSPDGKK LASGCKNGQV LLWDPSTGLQ VGRTLTGHSK
210 220 230 240 250
WITGLSWEPL HMNPECRYVA SSSKDGSVRV WDTTAGRCER ILTGHTQSVT
260 270 280 290 300
CLRWGGDGLL YSASQDRTIK VWRAHDGVLC RTLQGHGHWV NTMALSTDYA
310 320 330 340 350
LRTGAFEPAE ATVNAQDLQG SLKELKERAS SRYNLVRGQG PERLVSGSDD
360 370 380 390 400
FTLFLWSPAE DKKPLARMTG HQALINQVLF SPDSRIVASA SFDKSIKLWD
410 420 430 440 450
GRTGKYLASL RGHVAAVYQI AWSADSRLLV SGSSDSTLKV WDVKAQKLAT
460 470 480
DLPGHADEVY AVDWSPDGQR VASGGKDKCL RIWRR
Length:485
Mass (Da):53,134
Last modified:July 27, 2011 - v4
Checksum:iD0E7402BB0F44416
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6QAD9F6QAD9_MOUSE
Notchless protein homolog 1
Nle1
57Annotation score:
F7BBL8F7BBL8_MOUSE
Notchless protein homolog 1
Nle1
42Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180V → I in AAH18399 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603745 Genomic DNA No translation available.
BC018399 mRNA Translation: AAH18399.1
CCDSiCCDS25152.1
RefSeqiNP_663406.2, NM_145431.2
UniGeneiMm.29176

Genome annotation databases

EnsembliENSMUST00000103213; ENSMUSP00000099502; ENSMUSG00000020692
GeneIDi217011
KEGGimmu:217011
UCSCiuc007knt.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603745 Genomic DNA No translation available.
BC018399 mRNA Translation: AAH18399.1
CCDSiCCDS25152.1
RefSeqiNP_663406.2, NM_145431.2
UniGeneiMm.29176

3D structure databases

ProteinModelPortaliQ8VEJ4
SMRiQ8VEJ4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229831, 1 interactor
IntActiQ8VEJ4, 1 interactor
MINTiQ8VEJ4
STRINGi10090.ENSMUSP00000099502

PTM databases

iPTMnetiQ8VEJ4
PhosphoSitePlusiQ8VEJ4

Proteomic databases

EPDiQ8VEJ4
MaxQBiQ8VEJ4
PaxDbiQ8VEJ4
PeptideAtlasiQ8VEJ4
PRIDEiQ8VEJ4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103213; ENSMUSP00000099502; ENSMUSG00000020692
GeneIDi217011
KEGGimmu:217011
UCSCiuc007knt.2 mouse

Organism-specific databases

CTDi54475
MGIiMGI:2429770 Nle1

Phylogenomic databases

eggNOGiKOG0271 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091641
HOVERGENiHBG007602
InParanoidiQ8VEJ4
KOiK14855
OMAiLNTETPH
OrthoDBiEOG091G05DP
TreeFamiTF300668

Miscellaneous databases

PROiPR:Q8VEJ4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020692 Expressed in 160 organ(s), highest expression level in bone marrow
CleanExiMM_NLE1
ExpressionAtlasiQ8VEJ4 baseline and differential
GenevisibleiQ8VEJ4 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR012972 NLE
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF08154 NLE, 1 hit
PF00400 WD40, 7 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 8 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 7 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNLE1_MOUSE
AccessioniPrimary (citable) accession number: Q8VEJ4
Secondary accession number(s): B1ARD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 130 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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