UniProtKB - Q8VEI3 (HYAL3_MOUSE)
Protein
Hyaluronidase-3
Gene
Hyal3
Organism
Mus musculus (Mouse)
Status
Functioni
Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm (PubMed:20586096). Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage (PubMed:18653706). Has no hyaluronidase activity in embryonic fibroblasts in vitro (PubMed:18234732). Has no hyaluronidase activity in granulosa cells in vitro (PubMed:18653706).3 Publications
Catalytic activityi
- Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication EC:3.2.1.35
pH dependencei
Optimum pH is 7. Higher activity at pH 4 than at pH 7 in sperm.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 129 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- hyaluronoglucuronidase activity Source: MGI
- hyalurononglucosaminidase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- cartilage development Source: Ensembl
- cell adhesion Source: UniProtKB-KW
- cellular response to interleukin-1 Source: MGI
- cellular response to tumor necrosis factor Source: Ensembl
- cellular response to UV-B Source: MGI
- hyaluronan catabolic process Source: UniProtKB
- inflammatory response Source: MGI
- negative regulation of ovarian follicle development Source: UniProtKB
- ovarian follicle atresia Source: UniProtKB
- penetration of zona pellucida Source: UniProtKB
- positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
- response to antibiotic Source: Ensembl
- response to virus Source: UniProtKB
- viral entry into host cell Source: GOC
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell adhesion, Fertilization |
Enzyme and pathway databases
BRENDAi | 3.2.1.35, 3474 |
Reactomei | R-MMU-2024101, CS/DS degradation R-MMU-2160916, Hyaluronan uptake and degradation |
Protein family/group databases
CAZyi | GH56, Glycoside Hydrolase Family 56 |
Names & Taxonomyi
Protein namesi | Recommended name: Hyaluronidase-3 (EC:3.2.1.35)Short name: Hyal-3 Alternative name(s): Hyaluronoglucosaminidase-3 |
Gene namesi | Name:Hyal3 Synonyms:Hyl3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1330288, Hyal3 |
Subcellular locationi
Endosome
- Early endosome 1 Publication
Plasma membrane
- Cell membrane 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Extracellular region or secreted
- Secreted 1 Publication
Other locations
- acrosome 1 Publication
Note: Mostly present in low-density vesicles. Low levels in higher density vesicles of late endosomes and lysosomes. Localized in punctate cytoplasmic vesicles and in perinuclear structures, but does not colocalize with LAMP1 (PubMed:18234732). Localized on the plasma membrane over the acrosome and on the surface of the midpiece of the sperm tail (PubMed:20586096).2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Endosome
- early endosome Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Lysosome
- lysosome Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- acrosomal membrane Source: UniProtKB
- acrosomal vesicle Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB
- sperm midpiece Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
No visible phenotype. Both male and female mice are viable. Skeletal features, joints, whole-body weights, organ weights, organ morphologies and the serum hyaluronic acid (HA) levels are normal. No evidence of glycosaminoglycan accumulation, including vacuolization, in tissues analyzed including liver, lung, kidney, spleen, skin, fat, testes and seminal vesicles at 12-14 months of age. No difference in tissue organization or connective tissue thickness. Only a subtle change in the alveolar structure and extracellular matrix thickness in lung tissue sections at 12-14 months of age. Lungs have larger alveoli and more areas of thickened interstitium. Lung tissues from 6 months old mice show more immature alveoli compared to wild-type (PubMed:18762256). Mice are fully fertile (PubMed:18762256, PubMed:20586096). Sperm show delayed cumulus penetration and reduced acrosomal exocytosis (PubMed:20586096).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000248201 | 23 – 412 | Hyaluronidase-3Add BLAST | 390 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 332 | By similarity | ||
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 206 ↔ 221 | By similarity | ||
Glycosylationi | 216 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 357 ↔ 368 | By similarity | ||
Disulfide bondi | 362 ↔ 396 | By similarity | ||
Disulfide bondi | 398 ↔ 407 | By similarity |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q8VEI3 |
PRIDEi | Q8VEI3 |
ProteomicsDBi | 273289 |
PTM databases
GlyGeni | Q8VEI3, 2 sites |
Expressioni
Tissue specificityi
Expressed in testis, epididymal tissue, epididymal luminal fluid (ELF), acrosome-intact (AI) sperm and caput (CAP), corpus (COR) and caudal (CAU) sperm. Higher expression in sperm than testis (at protein level) (PubMed:20586096). Liver, kidney, skin, brain, stomach and testis (PubMed:11929860). Expressed mainly in granulosa cells of the ovaries. Expressed in small and large antral follicles. Not present in theca or stroma cells (PubMed:18653706). Expressed in testis and liver (PubMed:18762256). Expressed in testis and CAP, COR, and CAU epididymis tissue (PubMed:20586096).4 Publications
Inductioni
Up-regulated expression in apoptotic granulosa cells and in atretic follicles of the ovaries (PubMed:18653706).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000036091, Expressed in respiratory system and 69 other tissues |
ExpressionAtlasi | Q8VEI3, baseline and differential |
Genevisiblei | Q8VEI3, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000042667 |
Miscellaneous databases
RNActi | Q8VEI3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 353 – 408 | EGF-likeAdd BLAST | 56 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 56 family.Curated
Keywords - Domaini
EGF-like domain, SignalPhylogenomic databases
eggNOGi | ENOG502QTXP, Eukaryota |
GeneTreei | ENSGT01020000230364 |
HOGENOMi | CLU_036366_0_0_1 |
InParanoidi | Q8VEI3 |
OMAi | FYRFPAC |
OrthoDBi | 1096692at2759 |
PhylomeDBi | Q8VEI3 |
TreeFami | TF321598 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR000742, EGF-like_dom IPR013111, EGF_extracell IPR017853, Glycoside_hydrolase_SF IPR018155, Hyaluronidase IPR027260, Hyaluronidase-3 |
PANTHERi | PTHR11769, PTHR11769, 1 hit PTHR11769:SF19, PTHR11769:SF19, 1 hit |
Pfami | View protein in Pfam PF07974, EGF_2, 1 hit PF01630, Glyco_hydro_56, 1 hit |
PIRSFi | PIRSF038193, Hyaluronidase, 1 hit PIRSF500776, Hyaluronidase_3, 1 hit |
PRINTSi | PR00846, GLHYDRLASE56 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8VEI3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIMHLGLMMV VGLTLCLMHG QALLQVPEHP FSVVWNVPSA RCKAHFGVHL
60 70 80 90 100
PLDALGIVAN HGQHFHGQNI SIFYKNQFGL YPYFGPRGTA HNGGIPQAVS
110 120 130 140 150
LDHHLARAAH QILHSLGSSF AGLAVLDWEE WYPLWAGNWG PHRQVYLAAS
160 170 180 190 200
WVWTQQMFPG LDPQEQLHKA HTSFEQAARA LMEYTLQLGR TLRPSGLWGF
210 220 230 240 250
YRYPACGNGW HKMASNYTGH CHAAITTRNT QLRWLWAASS ALFPSIYLPP
260 270 280 290 300
RLPLAYRQAF VRHRLEEAFR VALLEHSHPL PVLAYSRLTH RSSGRFLSLD
310 320 330 340 350
DLMQTIGVSA ALGTAGVVLW GDLSFSSSEE KCWRLHDYLV GTLGPYVINV
360 370 380 390 400
TKAAMACSHQ RCHGHGRCAR KDPGQMEAFL HLQPDDSLGA WNSFRCHCYS
410
GWAGPTCLEP KP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YVY7 | D3YVY7_MOUSE | Hyaluronidase | Hyal3 | 328 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 228 | R → Q in AAL06145 (PubMed:11929860).Curated | 1 | |
Sequence conflicti | 228 | R → Q in AAH18457 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 296 | F → S in AAL54883 (Ref. 3) Curated | 1 | |
Sequence conflicti | 296 | F → S in AAL57175 (Ref. 3) Curated | 1 | |
Sequence conflicti | 354 | A → D in AAL06145 (PubMed:11929860).Curated | 1 | |
Sequence conflicti | 354 | A → D in AAH18457 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 397 | H → R in AAL54882 (Ref. 2) Curated | 1 | |
Sequence conflicti | 397 | H → R in AAL54883 (Ref. 3) Curated | 1 | |
Sequence conflicti | 397 | H → R in AAL57175 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY048681 mRNA Translation: AAL06145.1 AF074489 mRNA Translation: AAL54882.1 AF075576 Genomic DNA Translation: AAL54883.1 AF338323 Genomic DNA Translation: AAL57175.1 AL672219 Genomic DNA No translation available. BC018457 mRNA Translation: AAH18457.1 |
CCDSi | CCDS23499.1 |
RefSeqi | NP_821139.2, NM_178020.3 |
Genome annotation databases
Ensembli | ENSMUST00000040059; ENSMUSP00000042667; ENSMUSG00000036091 ENSMUST00000148440; ENSMUSP00000119499; ENSMUSG00000036091 |
GeneIDi | 109685 |
KEGGi | mmu:109685 |
UCSCi | uc009rmc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY048681 mRNA Translation: AAL06145.1 AF074489 mRNA Translation: AAL54882.1 AF075576 Genomic DNA Translation: AAL54883.1 AF338323 Genomic DNA Translation: AAL57175.1 AL672219 Genomic DNA No translation available. BC018457 mRNA Translation: AAH18457.1 |
CCDSi | CCDS23499.1 |
RefSeqi | NP_821139.2, NM_178020.3 |
3D structure databases
SMRi | Q8VEI3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000042667 |
Protein family/group databases
CAZyi | GH56, Glycoside Hydrolase Family 56 |
PTM databases
GlyGeni | Q8VEI3, 2 sites |
Proteomic databases
PaxDbi | Q8VEI3 |
PRIDEi | Q8VEI3 |
ProteomicsDBi | 273289 |
Protocols and materials databases
Antibodypediai | 34877, 188 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000040059; ENSMUSP00000042667; ENSMUSG00000036091 ENSMUST00000148440; ENSMUSP00000119499; ENSMUSG00000036091 |
GeneIDi | 109685 |
KEGGi | mmu:109685 |
UCSCi | uc009rmc.2, mouse |
Organism-specific databases
CTDi | 8372 |
MGIi | MGI:1330288, Hyal3 |
Phylogenomic databases
eggNOGi | ENOG502QTXP, Eukaryota |
GeneTreei | ENSGT01020000230364 |
HOGENOMi | CLU_036366_0_0_1 |
InParanoidi | Q8VEI3 |
OMAi | FYRFPAC |
OrthoDBi | 1096692at2759 |
PhylomeDBi | Q8VEI3 |
TreeFami | TF321598 |
Enzyme and pathway databases
BRENDAi | 3.2.1.35, 3474 |
Reactomei | R-MMU-2024101, CS/DS degradation R-MMU-2160916, Hyaluronan uptake and degradation |
Miscellaneous databases
BioGRID-ORCSi | 109685, 1 hit in 52 CRISPR screens |
PROi | PR:Q8VEI3 |
RNActi | Q8VEI3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036091, Expressed in respiratory system and 69 other tissues |
ExpressionAtlasi | Q8VEI3, baseline and differential |
Genevisiblei | Q8VEI3, MM |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR000742, EGF-like_dom IPR013111, EGF_extracell IPR017853, Glycoside_hydrolase_SF IPR018155, Hyaluronidase IPR027260, Hyaluronidase-3 |
PANTHERi | PTHR11769, PTHR11769, 1 hit PTHR11769:SF19, PTHR11769:SF19, 1 hit |
Pfami | View protein in Pfam PF07974, EGF_2, 1 hit PF01630, Glyco_hydro_56, 1 hit |
PIRSFi | PIRSF038193, Hyaluronidase, 1 hit PIRSF500776, Hyaluronidase_3, 1 hit |
PRINTSi | PR00846, GLHYDRLASE56 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HYAL3_MOUSE | |
Accessioni | Q8VEI3Primary (citable) accession number: Q8VEI3 Secondary accession number(s): B1AV95, Q8VBX7, Q8VI77 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families