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Protein

CCR4-NOT transcription complex subunit 6-like

Gene

Cnot6l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240By similarity1
Metal bindingi240Magnesium 1By similarity1
Metal bindingi410Magnesium 2By similarity1
Metal bindingi412Magnesium 2By similarity1
Active sitei489By similarity1
Active sitei529By similarity1
Metal bindingi529Magnesium 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • poly(A)-specific ribonuclease activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processmRNA processing, RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 6-like (EC:3.1.13.4)
Gene namesi
Name:Cnot6l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2443154 Cnot6l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003145881 – 555CCR4-NOT transcription complex subunit 6-likeAdd BLAST555

Proteomic databases

PaxDbiQ8VEG6
PeptideAtlasiQ8VEG6
PRIDEiQ8VEG6

PTM databases

iPTMnetiQ8VEG6
PhosphoSitePlusiQ8VEG6

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000034724 Expressed in 292 organ(s), highest expression level in primary oocyte
CleanExiMM_CNOT6L
ExpressionAtlasiQ8VEG6 baseline and differential
GenevisibleiQ8VEG6 MM

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (By similarity). Interacts with CNOT1, CNOT3, CNOT7, CNOT8 and CNOT9 (By similarity). Interacts with TOB1 (By similarity). Interacts with NANOS2 (PubMed:20133598). Interacts with ZFP36 (PubMed:21078877).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cnot7Q608092EBI-2104661,EBI-2104739

Protein-protein interaction databases

DIPiDIP-46842N
IntActiQ8VEG6, 5 interactors
MINTiQ8VEG6
STRINGi10090.ENSMUSP00000108629

Structurei

3D structure databases

ProteinModelPortaliQ8VEG6
SMRiQ8VEG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 78LRR 1Add BLAST22
Repeati80 – 101LRR 2Add BLAST22
Repeati103 – 125LRR 3Add BLAST23
Repeati126 – 148LRR 4Add BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 152Required for interaction with CNOT1, CNOT3 and CNOT7By similarityAdd BLAST152
Regioni158 – 555Nuclease domainBy similarityAdd BLAST398

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0620 Eukaryota
COG4886 LUCA
COG5239 LUCA
GeneTreeiENSGT00550000074364
HOGENOMiHOG000294222
HOVERGENiHBG052641
InParanoidiQ8VEG6
KOiK12603
OMAiIFNRVMP
OrthoDBiEOG091G05MA
PhylomeDBiQ8VEG6
TreeFamiTF323175

Family and domain databases

CDDicd10312 Deadenylase_CCR4b, 1 hit
Gene3Di3.60.10.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR034967 Deadenylase_CCR4b
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF13855 LRR_8, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 3 hits
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8VEG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRLIGMPKEK YDPPDPRRIY TIMSAEEVAN GKKSHWAELE ISGRVRSLST
60 70 80 90 100
SLWSLTHLTA LHLNDNNLAR IPPDIAKLHN LVYLDLSSNK LRSLPAELGN
110 120 130 140 150
MVSLRELLLN DNYLRVLPYE LGRLFQLQTL GLTGNPLSQD IMSLYQDPDG
160 170 180 190 200
TRKLLNFMLD NLAVHPEQLP PRPWITLKER DQILPSASFT VMCYNVLCDK
210 220 230 240 250
YATRQLYGYC PSWALNWEYR KKGIMEEIVN WDADIISLQE VETEQYFTLF
260 270 280 290 300
LPALKDRGYD GFFSPKSRAK IMSEQERKHV DGCAIFFKTE KFTLVQKHTV
310 320 330 340 350
EFNQVAMANS DGSEAMLNRV MTKDNIGVAV VLEVHKELFG TGMKPIHAAD
360 370 380 390 400
KQLLIVANAH MHWDPEYSDV KLIQTMMFVS EVKNILEKAS SRPGSPTADP
410 420 430 440 450
NSIPLVLCAD LNSLPDSGVV EYLSNGGVAD NHKDFKELRY NECLMNFSCS
460 470 480 490 500
GKNGSSEGRI THGFQLKSAY ENNLMPYTNY TFDFKGVIDY IFYSKTHMNV
510 520 530 540 550
LGVLGPLDPQ WLVENNITGC PHPHIPSDHF SLLTQLELHP PLLPLVNGVH

LPNRR
Length:555
Mass (Da):63,023
Last modified:January 15, 2008 - v2
Checksum:iFDC180DB315919DB
GO
Isoform 2 (identifier: Q8VEG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRLIGM → M

Show »
Length:550
Mass (Da):62,453
Checksum:i523CFA11207E304D
GO
Isoform 3 (identifier: Q8VEG6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRLIGM → M
     440-440: Y → V
     441-555: Missing.

Show »
Length:435
Mass (Da):49,397
Checksum:i2FC620EB985D4E14
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YVH6D3YVH6_MOUSE
CCR4-NOT transcription complex subu...
Cnot6l
99Annotation score:
D3YXZ0D3YXZ0_MOUSE
CCR4-NOT transcription complex subu...
Cnot6l
130Annotation score:
D3Z2G5D3Z2G5_MOUSE
CCR4-NOT transcription complex subu...
Cnot6l
87Annotation score:
F8WJG1F8WJG1_MOUSE
CCR4-NOT transcription complex subu...
Cnot6l
46Annotation score:
D3Z5R3D3Z5R3_MOUSE
CCR4-NOT transcription complex subu...
Cnot6l
55Annotation score:

Sequence cautioni

The sequence BAC26790 differs from that shown. Reason: Frameshift at positions 38 and 64.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti238L → F in BAE30646 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0303241 – 6MRLIGM → M in isoform 2 and isoform 3. 2 Publications6
Alternative sequenceiVSP_030325440Y → V in isoform 3. 1 Publication1
Alternative sequenceiVSP_030326441 – 555Missing in isoform 3. 1 PublicationAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030112 mRNA Translation: BAC26790.1 Frameshift.
AK151728 mRNA Translation: BAE30646.1
AC129600 Genomic DNA No translation available.
AC149285 Genomic DNA No translation available.
BC018506 mRNA Translation: AAH18506.1
CCDSiCCDS19448.1 [Q8VEG6-2]
CCDS51566.1 [Q8VEG6-1]
RefSeqiNP_001272440.1, NM_001285511.1 [Q8VEG6-2]
NP_001272443.1, NM_001285514.1
NP_659159.1, NM_144910.2 [Q8VEG6-2]
NP_849185.2, NM_178854.4 [Q8VEG6-1]
XP_006534933.1, XM_006534870.3 [Q8VEG6-2]
XP_006534934.1, XM_006534871.1 [Q8VEG6-2]
UniGeneiMm.28374
Mm.384746

Genome annotation databases

EnsembliENSMUST00000113005; ENSMUSP00000108629; ENSMUSG00000034724 [Q8VEG6-1]
ENSMUST00000122003; ENSMUSP00000113821; ENSMUSG00000034724 [Q8VEG6-3]
ENSMUST00000155901; ENSMUSP00000119415; ENSMUSG00000034724 [Q8VEG6-2]
GeneIDi231464
KEGGimmu:231464
UCSCiuc008yfd.2 mouse [Q8VEG6-3]
uc008yfe.2 mouse [Q8VEG6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030112 mRNA Translation: BAC26790.1 Frameshift.
AK151728 mRNA Translation: BAE30646.1
AC129600 Genomic DNA No translation available.
AC149285 Genomic DNA No translation available.
BC018506 mRNA Translation: AAH18506.1
CCDSiCCDS19448.1 [Q8VEG6-2]
CCDS51566.1 [Q8VEG6-1]
RefSeqiNP_001272440.1, NM_001285511.1 [Q8VEG6-2]
NP_001272443.1, NM_001285514.1
NP_659159.1, NM_144910.2 [Q8VEG6-2]
NP_849185.2, NM_178854.4 [Q8VEG6-1]
XP_006534933.1, XM_006534870.3 [Q8VEG6-2]
XP_006534934.1, XM_006534871.1 [Q8VEG6-2]
UniGeneiMm.28374
Mm.384746

3D structure databases

ProteinModelPortaliQ8VEG6
SMRiQ8VEG6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46842N
IntActiQ8VEG6, 5 interactors
MINTiQ8VEG6
STRINGi10090.ENSMUSP00000108629

PTM databases

iPTMnetiQ8VEG6
PhosphoSitePlusiQ8VEG6

Proteomic databases

PaxDbiQ8VEG6
PeptideAtlasiQ8VEG6
PRIDEiQ8VEG6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113005; ENSMUSP00000108629; ENSMUSG00000034724 [Q8VEG6-1]
ENSMUST00000122003; ENSMUSP00000113821; ENSMUSG00000034724 [Q8VEG6-3]
ENSMUST00000155901; ENSMUSP00000119415; ENSMUSG00000034724 [Q8VEG6-2]
GeneIDi231464
KEGGimmu:231464
UCSCiuc008yfd.2 mouse [Q8VEG6-3]
uc008yfe.2 mouse [Q8VEG6-1]

Organism-specific databases

CTDi246175
MGIiMGI:2443154 Cnot6l

Phylogenomic databases

eggNOGiKOG0620 Eukaryota
COG4886 LUCA
COG5239 LUCA
GeneTreeiENSGT00550000074364
HOGENOMiHOG000294222
HOVERGENiHBG052641
InParanoidiQ8VEG6
KOiK12603
OMAiIFNRVMP
OrthoDBiEOG091G05MA
PhylomeDBiQ8VEG6
TreeFamiTF323175

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Miscellaneous databases

ChiTaRSiCnot6l mouse
PROiPR:Q8VEG6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034724 Expressed in 292 organ(s), highest expression level in primary oocyte
CleanExiMM_CNOT6L
ExpressionAtlasiQ8VEG6 baseline and differential
GenevisibleiQ8VEG6 MM

Family and domain databases

CDDicd10312 Deadenylase_CCR4b, 1 hit
Gene3Di3.60.10.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR034967 Deadenylase_CCR4b
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF13855 LRR_8, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 3 hits
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCNO6L_MOUSE
AccessioniPrimary (citable) accession number: Q8VEG6
Secondary accession number(s): Q3U9M0, Q8C0P4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: September 12, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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