UniProtKB - Q8VEB6 (RNZ1_MOUSE)
Protein
Zinc phosphodiesterase ELAC protein 1
Gene
Elac1
Organism
Mus musculus (Mouse)
Status
Functioni
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity).By similarity
Catalytic activityi
- Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule. EC:3.1.26.11
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 62 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 64 | Zinc 1; catalyticBy similarity | 1 | |
Active sitei | 66 | Proton acceptorSequence analysis | 1 | |
Metal bindingi | 66 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 67 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 181 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 252 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 252 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 312 | Zinc 2; catalyticBy similarity | 1 |
GO - Molecular functioni
- 3'-tRNA processing endoribonuclease activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- tRNA 3'-trailer cleavage, endonucleolytic Source: GO_Central
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc phosphodiesterase ELAC protein 1 (EC:3.1.26.11)Alternative name(s): ElaC homolog protein 1 Ribonuclease Z 1 Short name: RNase Z 1 tRNA 3 endonuclease 1 tRNase Z 1 |
Gene namesi | Name:Elac1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890495, Elac1 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155826 | 1 – 362 | Zinc phosphodiesterase ELAC protein 1Add BLAST | 362 |
Proteomic databases
EPDi | Q8VEB6 |
PaxDbi | Q8VEB6 |
PRIDEi | Q8VEB6 |
PTM databases
PhosphoSitePlusi | Q8VEB6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036941, Expressed in retina and 250 other tissues |
Genevisiblei | Q8VEB6, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041793 |
Miscellaneous databases
RNActi | Q8VEB6, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the RNase Z family.Curated
Phylogenomic databases
eggNOGi | KOG2121, Eukaryota |
GeneTreei | ENSGT00730000111224 |
HOGENOMi | CLU_031317_2_2_1 |
InParanoidi | Q8VEB6 |
OMAi | GTQRQMM |
OrthoDBi | 1387038at2759 |
PhylomeDBi | Q8VEB6 |
TreeFami | TF324462 |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF00753, Lactamase_B, 1 hit PF12706, Lactamase_B_2, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8VEB6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSMDVTFLGT GAAYPSPTRG ASAVVLRCEG ECWLFDCGEG TQTQLMKSQL
60 70 80 90 100
KAGRITKIFI THLHGDHFFG LPGLLCTISL QSGSVVARQP IEIYGPVGLR
110 120 130 140 150
DFIWRTMELS HTELVFPYVV HELVPTADQC PVEELREFAH MDETDSSPKG
160 170 180 190 200
QGRTILLDAE ENSYCLVDDE QFVVKAFRLF HRIPSFGFSV VEKKRAGKLN
210 220 230 240 250
AQKLRDLGVP PGPAYGKLKN GISVVLDNGV TISPQDVLKK PMVGRKVCIL
260 270 280 290 300
GDCSGVVGDG GVKLCFEADL LIHEATLDDS QMDKAREHGH STPQMAAAFA
310 320 330 340 350
KLCRAKRLVL THFSQRYKPT ALAREGEADG IAELRKQAEA VLELQEVTLA
360
EDFMVIGIPI KK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 | T → P in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 263 | K → N in BAB31204 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 276 | T → G in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 282 | M → I in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 287 | E → D in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 303 | C → S in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 314 | S → I in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 321 | A → P in AAG24919 (PubMed:11175785).Curated | 1 | |
Sequence conflicti | 340 | A → P in AAG24919 (PubMed:11175785).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009167 | 1 – 241 | Missing in isoform 2. 1 PublicationAdd BLAST | 241 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF308697 mRNA Translation: AAG24919.1 AK018424 mRNA Translation: BAB31204.1 BC019371 mRNA No translation available. |
CCDSi | CCDS37855.1 [Q8VEB6-1] |
RefSeqi | NP_444485.2, NM_053255.3 [Q8VEB6-1] |
Genome annotation databases
Ensembli | ENSMUST00000041138; ENSMUSP00000041793; ENSMUSG00000036941 [Q8VEB6-1] |
GeneIDi | 114615 |
KEGGi | mmu:114615 |
UCSCi | uc008fow.1, mouse [Q8VEB6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF308697 mRNA Translation: AAG24919.1 AK018424 mRNA Translation: BAB31204.1 BC019371 mRNA No translation available. |
CCDSi | CCDS37855.1 [Q8VEB6-1] |
RefSeqi | NP_444485.2, NM_053255.3 [Q8VEB6-1] |
3D structure databases
SMRi | Q8VEB6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041793 |
PTM databases
PhosphoSitePlusi | Q8VEB6 |
Proteomic databases
EPDi | Q8VEB6 |
PaxDbi | Q8VEB6 |
PRIDEi | Q8VEB6 |
Protocols and materials databases
Antibodypediai | 22721, 233 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000041138; ENSMUSP00000041793; ENSMUSG00000036941 [Q8VEB6-1] |
GeneIDi | 114615 |
KEGGi | mmu:114615 |
UCSCi | uc008fow.1, mouse [Q8VEB6-1] |
Organism-specific databases
CTDi | 55520 |
MGIi | MGI:1890495, Elac1 |
Phylogenomic databases
eggNOGi | KOG2121, Eukaryota |
GeneTreei | ENSGT00730000111224 |
HOGENOMi | CLU_031317_2_2_1 |
InParanoidi | Q8VEB6 |
OMAi | GTQRQMM |
OrthoDBi | 1387038at2759 |
PhylomeDBi | Q8VEB6 |
TreeFami | TF324462 |
Miscellaneous databases
BioGRID-ORCSi | 114615, 0 hits in 17 CRISPR screens |
ChiTaRSi | Elac1, mouse |
PROi | PR:Q8VEB6 |
RNActi | Q8VEB6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036941, Expressed in retina and 250 other tissues |
Genevisiblei | Q8VEB6, MM |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF00753, Lactamase_B, 1 hit PF12706, Lactamase_B_2, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNZ1_MOUSE | |
Accessioni | Q8VEB6Primary (citable) accession number: Q8VEB6 Secondary accession number(s): Q9CXB1, Q9ERF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families