Pla2g15

Functionⁱ

Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (PubMed:11790796, PubMed:16106046, PubMed:16880524, PubMed:19017977, PubMed:20410020). Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), catalyzing the transfer of oleic acid to N-acetyl-sphingosine (PubMed:16880524). Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen (PubMed:16880524, PubMed:19017977).5 Publications

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	46	Substrate; via amide nitrogenBy similarity	1
Active siteⁱ	198	Acyl-ester intermediateSequence analysis2 Publications	1
Binding siteⁱ	199	Substrate; via amide nitrogenBy similarity	1
Active siteⁱ	360	Charge relay systemBy similarity1 Publication	1
Active siteⁱ	392	Charge relay systemBy similarity1 Publication	1

GO - Molecular functionⁱ

calcium-independent phospholipase A2 activity
Source: MGI
Inferred from direct assayⁱ
- 11790796
O-acyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 20410020

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ceramide metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 20410020
fatty acid metabolic process Source: UniProtKB-KW
glycerophospholipid metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 20410020
phosphatidylcholine catabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16880524
phosphatidylcholine metabolic process Source: MGI
phosphatidylethanolamine catabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16880524

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Acyltransferase, Hydrolase, Transferase
Biological process	Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1483115 Hydrolysis of LPC

Reactomeⁱ

R-MMU-1483115 Hydrolysis of LPC

Protein family/group databases

ESTHERⁱ	mouse-C87498 PC-sterol_acyltransferase

ESTHERⁱ

mouse-C87498 PC-sterol_acyltransferase

Chemistry databases

SwissLipidsⁱ	SLP:000000352

SwissLipidsⁱ

SLP:000000352

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Group XV phospholipase A2 (EC:2.3.1.-5 Publications) Alternative name(s): 1-O-acylceramide synthase1 Publication Short name: ACS LCAT-like lysophospholipase Short name: LLPL Lysophospholipase 3 Lysosomal phospholipase A22 Publications Short name: LPLA21 Publication
Gene namesⁱ	Name:Pla2g15 Synonyms:Lypla3
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:2178076 Pla2g15

Keywords - Cellular componentⁱ

Lysosome, Membrane, Secreted

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice are born at the expected Mendelian rate, are viable and fertile. They display strongly reduced transacylase activity in lung alveolar macrophages and in peritoneal macrophages, leading to the accumulation of phospholipids with phosphatidylethanolamine and phosphatidylcholine headgroups. Mice display higher numers of alveolar macrophages in the lung, together with a mononuclear cell infiltrate in airways and blood vessels. Alveolar nmacrophages are larger than normal and present lamellar inclusion bodies, indicative of cellular phospholipidosis. Besides, mutant mice display splenomegaly.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	65	C → A: Abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	89	C → A: Abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	198	S → A: Abolishes enzyme activity. 2 Publications	1
Mutagenesisⁱ	360	D → A: Abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	392	H → A: Abolishes enzyme activity. 1 Publication	1

Chemistry databases

ChEMBLⁱ

CHEMBL3259497

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 33	By similarityAdd BLAST		33
ChainⁱPRO_0000017809	34 – 412	Group XV phospholipase A2Add BLAST		379

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	65 ↔ 89	By similarity1 Publication
Glycosylationⁱ	99	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	273	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	289	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	398	N-linked (GlcNAc...) asparagineSequence analysis	1

Post-translational modificationⁱ

N-glycosylated (PubMed:11790796). N-glycosylation is important for maturation of the enzyme and normal subcellular location (By similarity).By similarity1 Publication

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

EPDⁱ	Q8VEB4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8VEB4
PaxDbⁱ	Q8VEB4
PeptideAtlas More...PeptideAtlasⁱ	Q8VEB4
PRIDEⁱ	Q8VEB4

PTM databases

PhosphoSitePlusⁱ	Q8VEB4

PhosphoSitePlusⁱ

Q8VEB4

Expressionⁱ

Tissue specificityⁱ

Detected in blood plasma (PubMed:20410020). Detected in alveolar macrophages (at protein level) (PubMed:16106046, PubMed:16880524, PubMed:19017977). Detected in heart, liver, spleen, kidney, thymus, brain and lung (PubMed:16880524).4 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000031903 Expressed in 243 organ(s), highest expression level in stroma of bone marrow
ExpressionAtlasⁱ	Q8VEB4 baseline and differential
Genevisibleⁱ	Q8VEB4 MM

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	228695, 1 interactor
IntActⁱ	Q8VEB4, 2 interactors
Molecular INTeraction database More...MINTⁱ	Q8VEB4
STRINGⁱ	10090.ENSMUSP00000034377

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8VEB4
SMRⁱ	Q8VEB4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG2369 Eukaryota ENOG410Y9CF LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004902
HOGENOMⁱ	HOG000238654
InParanoidⁱ	Q8VEB4
KEGG Orthology (KO) More...KOⁱ	K06129
OMAⁱ	PGVPLHC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G07S3
PhylomeDBⁱ	Q8VEB4
TreeFamⁱ	TF313258

Family and domain databases

InterProⁱ	View protein in InterPro IPR029058 AB_hydrolase IPR003386 LACT/PDAT_acylTrfase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02450 LCAT, 1 hit
SUPFAMⁱ	SSF53474 SSF53474, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q8VEB4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDRHLCTCRE TQLRSGLLLP LFLLMMLADL TLPAQRHPPV VLVPGDLGNQ 
        60         70         80         90        100
LEAKLDKPKV VHYLCSKKTD SYFTLWLNLE LLLPVIIDCW IDNIRLVYNR 
       110        120        130        140        150
TSRATQFPDG VDVRVPGFGE TFSMEFLDPS KRNVGSYFYT MVESLVGWGY 
       160        170        180        190        200
TRGEDVRGAP YDWRRAPNEN GPYFLALREM IEEMYQMYGG PVVLVAHSMG 
       210        220        230        240        250
NVYMLYFLQR QPQVWKDKYI HAFVSLGAPW GGVAKTLRVL ASGDNNRIPV 
       260        270        280        290        300
IGPLKIREQQ RSAVSTSWLL PYNHTWSHEK VFVYTPTTNY TLRDYHRFFR 
       310        320        330        340        350
DIGFEDGWFM RQDTEGLVEA MTPPGVELHC LYGTGVPTPN SFYYESFPDR 
       360        370        380        390        400
DPKICFGDGD GTVNLESVLQ CQAWQSRQEH RVSLQELPGS EHIEMLANAT 
       410 
TLAYLKRVLL EP

Length:412

Mass (Da):47,307

Last modified:March 1, 2002 - v1

Checksum:ⁱ6544369410C46C9A

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1D5RLZ5	A0A1D5RLZ5_MOUSE		Group XV phospholipase A2 Group XV phospholipase A2 (Lysophospholipase 3, isoform CRA_b)	Pla2g15 Lypla3, mCG_20474	236	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	342	F → S in BAE32987 (PubMed:16141072).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF468958 mRNA Translation: AAL78651.1 AY179884 Genomic DNA Translation: AAO49009.1 AK085194 mRNA Translation: BAC39387.1 AK155004 mRNA Translation: BAE32987.1 AK163111 mRNA Translation: BAE37197.1 AK170814 mRNA Translation: BAE42046.1 BC019373 mRNA Translation: AAH19373.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22630.1
NCBI Reference Sequences More...RefSeqⁱ	NP_598553.1, NM_133792.2
UniGeneⁱ	Mm.284770

Genome annotation databases

Ensemblⁱ	ENSMUST00000034377; ENSMUSP00000034377; ENSMUSG00000031903
GeneIDⁱ	192654
KEGGⁱ	mmu:192654
UCSC genome browser More...UCSCⁱ	uc009nfj.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8VEB4	Group XV phospholipase A2		RAT		413	UniRef90_Q8VEB4
group XV phospholipase A2		MESAU		412
Group XV phospholipase A2		CRIGR		412
UPI000B4F2C45		MERUN		419
UPI0003331391		JACJA		412
+4

Protein	Similar proteins		Species	Score	Length	Source
Q8VEB4	Group XV phospholipase A2		RAT		413	UniRef50_Q8VEB4
group XV phospholipase A2		MESAU		412
Group XV phospholipase A2		CRIGR		412
Phospholipase A2, group XV		LEPOC		420
Phospholipase A2, group XV		CALMI		414
+129

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF468958 mRNA Translation: AAL78651.1 AY179884 Genomic DNA Translation: AAO49009.1 AK085194 mRNA Translation: BAC39387.1 AK155004 mRNA Translation: BAE32987.1 AK163111 mRNA Translation: BAE37197.1 AK170814 mRNA Translation: BAE42046.1 BC019373 mRNA Translation: AAH19373.1
CCDSⁱ	CCDS22630.1
RefSeqⁱ	NP_598553.1, NM_133792.2
UniGeneⁱ	Mm.284770

3D structure databases

ProteinModelPortalⁱ	Q8VEB4
SMRⁱ	Q8VEB4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	228695, 1 interactor
IntActⁱ	Q8VEB4, 2 interactors
MINTⁱ	Q8VEB4
STRINGⁱ	10090.ENSMUSP00000034377

Chemistry databases

ChEMBLⁱ	CHEMBL3259497
SwissLipidsⁱ	SLP:000000352

Protein family/group databases

ESTHERⁱ	mouse-C87498 PC-sterol_acyltransferase

ESTHERⁱ

mouse-C87498 PC-sterol_acyltransferase

PTM databases

PhosphoSitePlusⁱ	Q8VEB4

PhosphoSitePlusⁱ

Q8VEB4

Proteomic databases

EPDⁱ	Q8VEB4
MaxQBⁱ	Q8VEB4
PaxDbⁱ	Q8VEB4
PeptideAtlasⁱ	Q8VEB4
PRIDEⁱ	Q8VEB4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034377; ENSMUSP00000034377; ENSMUSG00000031903
GeneIDⁱ	192654
KEGGⁱ	mmu:192654
UCSCⁱ	uc009nfj.1 mouse

Organism-specific databases

CTDⁱ	23659
MGIⁱ	MGI:2178076 Pla2g15

Phylogenomic databases

eggNOGⁱ	KOG2369 Eukaryota ENOG410Y9CF LUCA
GeneTreeⁱ	ENSGT00390000004902
HOGENOMⁱ	HOG000238654
InParanoidⁱ	Q8VEB4
KOⁱ	K06129
OMAⁱ	PGVPLHC
OrthoDBⁱ	EOG091G07S3
PhylomeDBⁱ	Q8VEB4
TreeFamⁱ	TF313258

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1483115 Hydrolysis of LPC

Reactomeⁱ

R-MMU-1483115 Hydrolysis of LPC

Miscellaneous databases

ChiTaRSⁱ	Pla2g15 mouse
Protein Ontology More...PROⁱ	PR:Q8VEB4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031903 Expressed in 243 organ(s), highest expression level in stroma of bone marrow
ExpressionAtlasⁱ	Q8VEB4 baseline and differential
Genevisibleⁱ	Q8VEB4 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR029058 AB_hydrolase IPR003386 LACT/PDAT_acylTrfase
Pfamⁱ	View protein in Pfam PF02450 LCAT, 1 hit
SUPFAMⁱ	SSF53474 SSF53474, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PAG15_MOUSE
Accessionⁱ	Q8VEB4Primary (citable) accession number: Q8VEB4 Secondary accession number(s): Q3TCB1, Q3U303
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
	Last sequence update:	March 1, 2002
	Last modified:	November 7, 2018
	This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8VEB4 (PAG15_MOUSE)

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Group XV phospholipase A2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Lysosome

Extracellular region or secreted

Other locations

Extracellular region or secreted

Lysosome

Mitochondrion

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ