UniProtKB - Q8VE73 (CUL7_MOUSE)
Cullin-7
Cul7
Functioni
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ubiquitin protein ligase binding Source: InterPro
GO - Biological processi
- epithelial to mesenchymal transition Source: UniProtKB
- Golgi organization Source: UniProtKB
- microtubule cytoskeleton organization Source: UniProtKB
- mitotic cytokinesis Source: UniProtKB
- placenta development Source: UniProtKB
- positive regulation of dendrite morphogenesis Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of endothelial cell differentiation Source: UniProtKB
- regulation of mitotic nuclear division Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: InterPro
- vasculogenesis Source: UniProtKB
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-8951664, Neddylation R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Cullin-7Short name: CUL-7 Alternative name(s): p185 p193 |
Gene namesi | Name:Cul7 Synonyms:Kiaa0076 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913765, Cul7 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Golgi apparatus
- Golgi apparatus By similarity
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
Note: Colocalizes with FBXW8 at the Golgi apparatus in neurons; localization to Golgi is mediated by OBSL1. During mitosis, localizes to the mitotic apparatus. CCDC8 is required for centrosomal location (By similarity).By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Other locations
- 3M complex Source: UniProtKB
- Cul7-RING ubiquitin ligase complex Source: UniProtKB
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Golgi apparatusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119803 | 1 – 1689 | Cullin-7Add BLAST | 1689 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 1567 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)Sequence analysis |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q8VE73 |
PRIDEi | Q8VE73 |
PTM databases
iPTMneti | Q8VE73 |
PhosphoSitePlusi | Q8VE73 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000038545, Expressed in membranous labyrinth and 281 other tissues |
ExpressionAtlasi | Q8VE73, baseline and differential |
Genevisiblei | Q8VE73, MM |
Interactioni
Subunit structurei
Component of the 3M complex, composed of core components CUL7, CCDC8 and OBSL1.
Part of a Cul7-RING complex consisting of CUL7, RBX1, SKP1 and FBXW8.
Interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.
Interacts with CUL9; leading to inhibit CUL9 activity.
Interacts with FBXW8; interaction is mutually exclusive of binding to CUL9 or p53/TP53.
Interacts with p53/TP53; the interaction preferentially involves tetrameric and dimeric p53/TP53. The CUL7-CUL9 heterodimer seems to interact specifically with p53/TP53.
Interacts with OBSL1 (By similarity).
Interacts with CUL1; the interactions seems to be mediated by FBXW8.
Interacts (as part of the 3M complex) with HDAC4 and HDAC5; it is negatively regulated by ANKRA2 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- ubiquitin protein ligase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 211530, 13 interactors |
CORUMi | Q8VE73 |
STRINGi | 10090.ENSMUSP00000049128 |
Miscellaneous databases
RNActi | Q8VE73, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 793 – 972 | DOCPROSITE-ProRule annotationAdd BLAST | 180 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 349 – 449 | Interaction with TP53By similarityAdd BLAST | 101 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1308 – 1370 | Glu-richAdd BLAST | 63 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502RDJD, Eukaryota |
GeneTreei | ENSGT00940000153954 |
HOGENOMi | CLU_001067_1_0_1 |
InParanoidi | Q8VE73 |
OMAi | NCLVVRI |
PhylomeDBi | Q8VE73 |
TreeFami | TF101154 |
Family and domain databases
Gene3Di | 2.30.30.30, 1 hit 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR004939, APC_su10/DOC_dom IPR016024, ARM-type_fold IPR021097, CPH_domain IPR031223, CUL7 IPR016157, Cullin_CS IPR016158, Cullin_homology IPR036317, Cullin_homology_sf IPR001373, Cullin_N IPR019559, Cullin_neddylation_domain IPR008979, Galactose-bd-like_sf IPR014722, Rib_L2_dom2 |
PANTHERi | PTHR22771:SF3, PTHR22771:SF3, 1 hit |
Pfami | View protein in Pfam PF03256, ANAPC10, 1 hit PF11515, Cul7, 1 hit PF00888, Cullin, 1 hit |
SMARTi | View protein in SMART SM01337, APC10, 1 hit SM00884, Cullin_Nedd8, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49785, SSF49785, 1 hit SSF75632, SSF75632, 1 hit |
PROSITEi | View protein in PROSITE PS01256, CULLIN_1, 1 hit PS50069, CULLIN_2, 1 hit PS51284, DOC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVGELRYREF RVPLGPGLHA YPDELIRQRV GHNGHPEYQI RWLILRRGDD
60 70 80 90 100
GDRDSTVDCK AEHILLWMSD DEIYANCHKM LGENGQVIAP SRESTEAGAL
110 120 130 140 150
DKSVLGEMET DVKSLIQRAL RQLEECVGTV PPAPLLHTVH VLSAYASIEP
160 170 180 190 200
LTGIFKDRRV VNLLMHMLSS PDYQIRWSAG RMIQALSSHD AGTRTQILLS
210 220 230 240 250
LSQQEAIEKH LDFDSRCALL ALFAQATLTE HPMSFEGVQL PQVPGRLLFS
260 270 280 290 300
LVKRYLHVTF LLDRLNGDAG DQGAQNNFIP EELNVGRGRL ELEFSMAMGT
310 320 330 340 350
LISELVQAMR WDGASSRPES SSSSTFQPRP AQFRPYTQRF RRSRRFRPRA
360 370 380 390 400
SFASFNTYAL YVRDTLRPGM RVRMLENYEE IAAGDEGQFR QSNDGVPPAQ
410 420 430 440 450
VLWDSTGHTY WVHWHMLEIL GFEEDIEDVI DIEELQELGA NGALSIVPPS
460 470 480 490 500
QRWKPITQLF AEPYVVPEEE DREESENLTQ AEWWELLFFI RQLSEAERLH
510 520 530 540 550
IVDLLQDHLE EERVLDYDML PELTVPVDLA QDLLLSLPQQ LEDSALRDLF
560 570 580 590 600
SCSVYRKYGP EVLVGHLSYP FVPGAQPNLF GANEESEAKD PPLQSASPAL
610 620 630 640 650
QRLVESLGPE GEVLVELEQA LGSEAPQETE VKSCLLQLQE QPQPFLALMR
660 670 680 690 700
SLDTSASNKT LHLTVLRILM QLVNFPEALL LPWHEAMDAC VTCLRSPNTD
710 720 730 740 750
REVLQELIFF LHRLTTTSRD YAVILNQLGA RDAISKVLEK HRGKLELAQE
760 770 780 790 800
LRDMVSKCEK HAHLYRKLTT NILGGCIQMV LGQIEDHRRT HRPIQIPFFD
810 820 830 840 850
VFLRYLCQGS SEEMKKNRYW EKVEVSSNPQ RASRLTDRNP KTYWESSGRA
860 870 880 890 900
GSHFITLHMR PGVIIRQLTL LVAGEDSSYM PAWVVVCGGN SIKSVNKELN
910 920 930 940 950
TVNVMPSASR VTLLENLTRF WPIIQIRIKR CQQGGINTRI RGLEVLGPKP
960 970 980 990 1000
TFWPVFREQL CRHTRLFYMV RAQAWSQDIA EDRRSLLHLS SRLNGALRHE
1010 1020 1030 1040 1050
QNFAERFLPD MEAAQALSKT CWEALVSPLV QNITSPDEDS TSSLGWLLDQ
1060 1070 1080 1090 1100
YLGCREAAYN PQSRAAAFSS RVRRLTHLLV HVEPREAAPP VVAIPRSKGR
1110 1120 1130 1140 1150
NRIHDWSYLI TRGLPSSIMK NLTRCWRSVV EEQMNKFLTA SWKDDDFVPR
1160 1170 1180 1190 1200
YCERYYVLQK SSSELFGPRA AFLLAMRNGC ADAVLRLPFL RAAHVSEQFA
1210 1220 1230 1240 1250
RHIDQRIQGS RMGGARGMEM LAQLQRCLES VLIFSPLEIA TTFEHYYQHY
1260 1270 1280 1290 1300
MADRLLSVGS SWLEGAVLEQ IGPCFPSRLP QQMLQSLNVS EELQRQFHVY
1310 1320 1330 1340 1350
QLQQLDQELL KLEDTEKKIQ VAHEDSGRED KSKKEEAIGE AAAVAMAEEE
1360 1370 1380 1390 1400
DQGKKEEGEE EGEGEDEEEE RYYKGTMPEV CVLVVTPRFW PVASVCQMLN
1410 1420 1430 1440 1450
PATCLPAYLR GTINHYTNFY SKSQSRSSLE KEPQRRLQWT WQGRAEVQFG
1460 1470 1480 1490 1500
GQILHVSTVQ MWLLLHLNNQ KEVSVESLQA ISELPPDVLH RAIGPLTSSR
1510 1520 1530 1540 1550
GPLDLQEQKN VPGGVLKIRD DSEEPRPRRG NVWLIPPQTY LQAEAEEGRN
1560 1570 1580 1590 1600
MEKRRNLLNC LVVRILKAHG DEGLHVDRLV YLVLEAWEKG PCPARGLVSS
1610 1620 1630 1640 1650
LGRGATCRSS DVLSCILHLL VKGTLRRHDD RPQVLYYAVP VTVMEPHMES
1660 1670 1680
LNPGSAGPNP PLTFHTLQIR SRGVPYASCT DNHTFSTFR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q6H4 | E9Q6H4_MOUSE | Cullin-7 | Cul7 | 649 | Annotation score: | ||
F6Q1R4 | F6Q1R4_MOUSE | Cullin-7 | Cul7 | 577 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 395 | G → V in BAE27723 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 518 | D → E in BAD32160 (PubMed:15368895).Curated | 1 | |
Sequence conflicti | 777 – 779 | IQM → DAW in AAH19645 (PubMed:15489334).Curated | 3 | |
Sequence conflicti | 855 – 922 | Missing in AAH59865 (PubMed:15489334).CuratedAdd BLAST | 68 | |
Sequence conflicti | 888 | G → R in BAE27723 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 959 | Q → E in AAH59865 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1482 | S → L in BAB25811 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1495 | P → L in BAB25811 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038331 | 1472 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008655 mRNA Translation: BAB25811.1 AK147156 mRNA Translation: BAE27723.1 CT030702 Genomic DNA No translation available. BC019645 mRNA Translation: AAH19645.1 BC026946 mRNA Translation: AAH26946.2 BC059865 mRNA Translation: AAH59865.1 BC100544 mRNA Translation: AAI00545.1 AK172882 mRNA Translation: BAD32160.1 |
CCDSi | CCDS28833.1 [Q8VE73-1] |
RefSeqi | NP_079887.3, NM_025611.5 [Q8VE73-1] |
Genome annotation databases
Ensembli | ENSMUST00000043464; ENSMUSP00000049128; ENSMUSG00000038545 [Q8VE73-1] |
GeneIDi | 66515 |
KEGGi | mmu:66515 |
UCSCi | uc008ctp.1, mouse [Q8VE73-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008655 mRNA Translation: BAB25811.1 AK147156 mRNA Translation: BAE27723.1 CT030702 Genomic DNA No translation available. BC019645 mRNA Translation: AAH19645.1 BC026946 mRNA Translation: AAH26946.2 BC059865 mRNA Translation: AAH59865.1 BC100544 mRNA Translation: AAI00545.1 AK172882 mRNA Translation: BAD32160.1 |
CCDSi | CCDS28833.1 [Q8VE73-1] |
RefSeqi | NP_079887.3, NM_025611.5 [Q8VE73-1] |
3D structure databases
SMRi | Q8VE73 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211530, 13 interactors |
CORUMi | Q8VE73 |
STRINGi | 10090.ENSMUSP00000049128 |
PTM databases
iPTMneti | Q8VE73 |
PhosphoSitePlusi | Q8VE73 |
Proteomic databases
PaxDbi | Q8VE73 |
PRIDEi | Q8VE73 |
Protocols and materials databases
Antibodypediai | 4223, 185 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043464; ENSMUSP00000049128; ENSMUSG00000038545 [Q8VE73-1] |
GeneIDi | 66515 |
KEGGi | mmu:66515 |
UCSCi | uc008ctp.1, mouse [Q8VE73-1] |
Organism-specific databases
CTDi | 9820 |
MGIi | MGI:1913765, Cul7 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502RDJD, Eukaryota |
GeneTreei | ENSGT00940000153954 |
HOGENOMi | CLU_001067_1_0_1 |
InParanoidi | Q8VE73 |
OMAi | NCLVVRI |
PhylomeDBi | Q8VE73 |
TreeFami | TF101154 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-8951664, Neddylation R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 66515, 0 hits in 17 CRISPR screens |
ChiTaRSi | Cul7, mouse |
PROi | PR:Q8VE73 |
RNActi | Q8VE73, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038545, Expressed in membranous labyrinth and 281 other tissues |
ExpressionAtlasi | Q8VE73, baseline and differential |
Genevisiblei | Q8VE73, MM |
Family and domain databases
Gene3Di | 2.30.30.30, 1 hit 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR004939, APC_su10/DOC_dom IPR016024, ARM-type_fold IPR021097, CPH_domain IPR031223, CUL7 IPR016157, Cullin_CS IPR016158, Cullin_homology IPR036317, Cullin_homology_sf IPR001373, Cullin_N IPR019559, Cullin_neddylation_domain IPR008979, Galactose-bd-like_sf IPR014722, Rib_L2_dom2 |
PANTHERi | PTHR22771:SF3, PTHR22771:SF3, 1 hit |
Pfami | View protein in Pfam PF03256, ANAPC10, 1 hit PF11515, Cul7, 1 hit PF00888, Cullin, 1 hit |
SMARTi | View protein in SMART SM01337, APC10, 1 hit SM00884, Cullin_Nedd8, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49785, SSF49785, 1 hit SSF75632, SSF75632, 1 hit |
PROSITEi | View protein in PROSITE PS01256, CULLIN_1, 1 hit PS50069, CULLIN_2, 1 hit PS51284, DOC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CUL7_MOUSE | |
Accessioni | Q8VE73Primary (citable) accession number: Q8VE73 Secondary accession number(s): Q3UHY3 Q9CVD5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 7, 2003 |
Last sequence update: | January 9, 2007 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families