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Protein

Cullin-7

Gene

Cul7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5. Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1. Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development. Does not promote polyubiquitination and proteasomal degradation of p53/TP53. While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions (By similarity). Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-7
Short name:
CUL-7
Alternative name(s):
p185
p193
Gene namesi
Name:Cul7
Synonyms:Kiaa0076
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1913765 Cul7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Mice are neonatal lethal and show vascular defects in both the embryo and the placenta.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198031 – 1689Cullin-7Add BLAST1689

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki1567Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)Sequence analysis

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ8VE73
PRIDEiQ8VE73

PTM databases

iPTMnetiQ8VE73
PhosphoSitePlusiQ8VE73

Expressioni

Gene expression databases

BgeeiENSMUSG00000038545 Expressed in 265 organ(s), highest expression level in membranous labyrinth
CleanExiMM_CUL7
ExpressionAtlasiQ8VE73 baseline and differential
GenevisibleiQ8VE73 MM

Interactioni

Subunit structurei

Component of the 3M complex, composed of core components CUL7, CCDC8 and OBSL1. Part of a Cul7-RING complex consisting of CUL7, RBX1, SKP1 and FBXW8. Interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone. Interacts with CUL9; leading to inhibit CUL9 activity. Interacts with FBXW8; interaction is mutually exclusive of binding to CUL9 or p53/TP53. Interacts with p53/TP53; the interaction preferentially involves tetrameric and dimeric p53/TP53. The CUL7-CUL9 heterodimer seems to interact specifically with p53/TP53. Interacts with OBSL1 (By similarity). Interacts with CUL1; the interactions seems to be mediated by FBXW8. Interacts (as part of the 3M complex) with HDAC4 and HDAC5; it is negatively regulated by ANKRA2 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211530, 7 interactors
CORUMiQ8VE73
STRINGi10090.ENSMUSP00000049128

Structurei

3D structure databases

ProteinModelPortaliQ8VE73
SMRiQ8VE73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini793 – 972DOCPROSITE-ProRule annotationAdd BLAST180

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 449Interaction with TP53By similarityAdd BLAST101

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1308 – 1370Glu-richAdd BLAST63

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00890000139411
HOVERGENiHBG103792
InParanoidiQ8VE73
KOiK10613
OMAiWKPMTEL
OrthoDBiEOG091G0053
PhylomeDBiQ8VE73
TreeFamiTF101154

Family and domain databases

Gene3Di2.30.30.30, 1 hit
2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR004939 APC_su10/DOC_dom
IPR016024 ARM-type_fold
IPR021097 CPH_domain
IPR031223 CUL7
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR008979 Galactose-bd-like_sf
IPR014722 Rib_L2_dom2
PANTHERiPTHR22771:SF3 PTHR22771:SF3, 1 hit
PfamiView protein in Pfam
PF03256 ANAPC10, 1 hit
PF11515 Cul7, 1 hit
PF00888 Cullin, 1 hit
SMARTiView protein in SMART
SM01337 APC10, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF49785 SSF49785, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit
PS51284 DOC, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8VE73-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVGELRYREF RVPLGPGLHA YPDELIRQRV GHNGHPEYQI RWLILRRGDD
60 70 80 90 100
GDRDSTVDCK AEHILLWMSD DEIYANCHKM LGENGQVIAP SRESTEAGAL
110 120 130 140 150
DKSVLGEMET DVKSLIQRAL RQLEECVGTV PPAPLLHTVH VLSAYASIEP
160 170 180 190 200
LTGIFKDRRV VNLLMHMLSS PDYQIRWSAG RMIQALSSHD AGTRTQILLS
210 220 230 240 250
LSQQEAIEKH LDFDSRCALL ALFAQATLTE HPMSFEGVQL PQVPGRLLFS
260 270 280 290 300
LVKRYLHVTF LLDRLNGDAG DQGAQNNFIP EELNVGRGRL ELEFSMAMGT
310 320 330 340 350
LISELVQAMR WDGASSRPES SSSSTFQPRP AQFRPYTQRF RRSRRFRPRA
360 370 380 390 400
SFASFNTYAL YVRDTLRPGM RVRMLENYEE IAAGDEGQFR QSNDGVPPAQ
410 420 430 440 450
VLWDSTGHTY WVHWHMLEIL GFEEDIEDVI DIEELQELGA NGALSIVPPS
460 470 480 490 500
QRWKPITQLF AEPYVVPEEE DREESENLTQ AEWWELLFFI RQLSEAERLH
510 520 530 540 550
IVDLLQDHLE EERVLDYDML PELTVPVDLA QDLLLSLPQQ LEDSALRDLF
560 570 580 590 600
SCSVYRKYGP EVLVGHLSYP FVPGAQPNLF GANEESEAKD PPLQSASPAL
610 620 630 640 650
QRLVESLGPE GEVLVELEQA LGSEAPQETE VKSCLLQLQE QPQPFLALMR
660 670 680 690 700
SLDTSASNKT LHLTVLRILM QLVNFPEALL LPWHEAMDAC VTCLRSPNTD
710 720 730 740 750
REVLQELIFF LHRLTTTSRD YAVILNQLGA RDAISKVLEK HRGKLELAQE
760 770 780 790 800
LRDMVSKCEK HAHLYRKLTT NILGGCIQMV LGQIEDHRRT HRPIQIPFFD
810 820 830 840 850
VFLRYLCQGS SEEMKKNRYW EKVEVSSNPQ RASRLTDRNP KTYWESSGRA
860 870 880 890 900
GSHFITLHMR PGVIIRQLTL LVAGEDSSYM PAWVVVCGGN SIKSVNKELN
910 920 930 940 950
TVNVMPSASR VTLLENLTRF WPIIQIRIKR CQQGGINTRI RGLEVLGPKP
960 970 980 990 1000
TFWPVFREQL CRHTRLFYMV RAQAWSQDIA EDRRSLLHLS SRLNGALRHE
1010 1020 1030 1040 1050
QNFAERFLPD MEAAQALSKT CWEALVSPLV QNITSPDEDS TSSLGWLLDQ
1060 1070 1080 1090 1100
YLGCREAAYN PQSRAAAFSS RVRRLTHLLV HVEPREAAPP VVAIPRSKGR
1110 1120 1130 1140 1150
NRIHDWSYLI TRGLPSSIMK NLTRCWRSVV EEQMNKFLTA SWKDDDFVPR
1160 1170 1180 1190 1200
YCERYYVLQK SSSELFGPRA AFLLAMRNGC ADAVLRLPFL RAAHVSEQFA
1210 1220 1230 1240 1250
RHIDQRIQGS RMGGARGMEM LAQLQRCLES VLIFSPLEIA TTFEHYYQHY
1260 1270 1280 1290 1300
MADRLLSVGS SWLEGAVLEQ IGPCFPSRLP QQMLQSLNVS EELQRQFHVY
1310 1320 1330 1340 1350
QLQQLDQELL KLEDTEKKIQ VAHEDSGRED KSKKEEAIGE AAAVAMAEEE
1360 1370 1380 1390 1400
DQGKKEEGEE EGEGEDEEEE RYYKGTMPEV CVLVVTPRFW PVASVCQMLN
1410 1420 1430 1440 1450
PATCLPAYLR GTINHYTNFY SKSQSRSSLE KEPQRRLQWT WQGRAEVQFG
1460 1470 1480 1490 1500
GQILHVSTVQ MWLLLHLNNQ KEVSVESLQA ISELPPDVLH RAIGPLTSSR
1510 1520 1530 1540 1550
GPLDLQEQKN VPGGVLKIRD DSEEPRPRRG NVWLIPPQTY LQAEAEEGRN
1560 1570 1580 1590 1600
MEKRRNLLNC LVVRILKAHG DEGLHVDRLV YLVLEAWEKG PCPARGLVSS
1610 1620 1630 1640 1650
LGRGATCRSS DVLSCILHLL VKGTLRRHDD RPQVLYYAVP VTVMEPHMES
1660 1670 1680
LNPGSAGPNP PLTFHTLQIR SRGVPYASCT DNHTFSTFR
Length:1,689
Mass (Da):192,292
Last modified:January 9, 2007 - v2
Checksum:i6ED4641B577E53CD
GO
Isoform 2 (identifier: Q8VE73-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1472-1472: Missing.

Note: No experimental confirmation available.
Show »
Length:1,688
Mass (Da):192,163
Checksum:i8AD13BBC66EAA8A5
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Q1R4F6Q1R4_MOUSE
Cullin-7
Cul7
577Annotation score:
E9Q6H4E9Q6H4_MOUSE
Cullin-7
Cul7
649Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti395G → V in BAE27723 (PubMed:16141072).Curated1
Sequence conflicti518D → E in BAD32160 (PubMed:15368895).Curated1
Sequence conflicti777 – 779IQM → DAW in AAH19645 (PubMed:15489334).Curated3
Sequence conflicti855 – 922Missing in AAH59865 (PubMed:15489334).CuratedAdd BLAST68
Sequence conflicti888G → R in BAE27723 (PubMed:16141072).Curated1
Sequence conflicti959Q → E in AAH59865 (PubMed:15489334).Curated1
Sequence conflicti1482S → L in BAB25811 (PubMed:16141072).Curated1
Sequence conflicti1495P → L in BAB25811 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0383311472Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008655 mRNA Translation: BAB25811.1
AK147156 mRNA Translation: BAE27723.1
CT030702 Genomic DNA No translation available.
BC019645 mRNA Translation: AAH19645.1
BC026946 mRNA Translation: AAH26946.2
BC059865 mRNA Translation: AAH59865.1
BC100544 mRNA Translation: AAI00545.1
AK172882 mRNA Translation: BAD32160.1
CCDSiCCDS28833.1 [Q8VE73-1]
RefSeqiNP_079887.3, NM_025611.5 [Q8VE73-1]
UniGeneiMm.329078

Genome annotation databases

EnsembliENSMUST00000043464; ENSMUSP00000049128; ENSMUSG00000038545 [Q8VE73-1]
GeneIDi66515
KEGGimmu:66515
UCSCiuc008ctp.1 mouse [Q8VE73-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008655 mRNA Translation: BAB25811.1
AK147156 mRNA Translation: BAE27723.1
CT030702 Genomic DNA No translation available.
BC019645 mRNA Translation: AAH19645.1
BC026946 mRNA Translation: AAH26946.2
BC059865 mRNA Translation: AAH59865.1
BC100544 mRNA Translation: AAI00545.1
AK172882 mRNA Translation: BAD32160.1
CCDSiCCDS28833.1 [Q8VE73-1]
RefSeqiNP_079887.3, NM_025611.5 [Q8VE73-1]
UniGeneiMm.329078

3D structure databases

ProteinModelPortaliQ8VE73
SMRiQ8VE73
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211530, 7 interactors
CORUMiQ8VE73
STRINGi10090.ENSMUSP00000049128

PTM databases

iPTMnetiQ8VE73
PhosphoSitePlusiQ8VE73

Proteomic databases

PaxDbiQ8VE73
PRIDEiQ8VE73

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043464; ENSMUSP00000049128; ENSMUSG00000038545 [Q8VE73-1]
GeneIDi66515
KEGGimmu:66515
UCSCiuc008ctp.1 mouse [Q8VE73-1]

Organism-specific databases

CTDi9820
MGIiMGI:1913765 Cul7
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00890000139411
HOVERGENiHBG103792
InParanoidiQ8VE73
KOiK10613
OMAiWKPMTEL
OrthoDBiEOG091G0053
PhylomeDBiQ8VE73
TreeFamiTF101154

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiCul7 mouse
PROiPR:Q8VE73
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038545 Expressed in 265 organ(s), highest expression level in membranous labyrinth
CleanExiMM_CUL7
ExpressionAtlasiQ8VE73 baseline and differential
GenevisibleiQ8VE73 MM

Family and domain databases

Gene3Di2.30.30.30, 1 hit
2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR004939 APC_su10/DOC_dom
IPR016024 ARM-type_fold
IPR021097 CPH_domain
IPR031223 CUL7
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR008979 Galactose-bd-like_sf
IPR014722 Rib_L2_dom2
PANTHERiPTHR22771:SF3 PTHR22771:SF3, 1 hit
PfamiView protein in Pfam
PF03256 ANAPC10, 1 hit
PF11515 Cul7, 1 hit
PF00888 Cullin, 1 hit
SMARTiView protein in SMART
SM01337 APC10, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF49785 SSF49785, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit
PS51284 DOC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCUL7_MOUSE
AccessioniPrimary (citable) accession number: Q8VE73
Secondary accession number(s): Q3UHY3
, Q497I2, Q6A0D6, Q6PB63, Q8R3W4, Q9CVD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: January 9, 2007
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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