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Protein

Ubiquitin-like modifier-activating enzyme 5

Gene

Uba5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E1-like enzyme which activates UFM1 and SUMO2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81ATP; via amide nitrogenBy similarity1
Binding sitei102ATPBy similarity1
Binding sitei125ATPBy similarity1
Binding sitei148ATPBy similarity1
Binding sitei182ATPBy similarity1
Metal bindingi224ZincBy similarity1
Metal bindingi227ZincBy similarity1
Active sitei248Glycyl thioester intermediateBy similarity1
Metal bindingi301ZincBy similarity1
Metal bindingi306ZincBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • UFM1 activating enzyme activity Source: MGI

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 5
Short name:
Ubiquitin-activating enzyme 5
Alternative name(s):
UFM1-activating enzyme
Ubiquitin-activating enzyme E1 domain-containing protein 1
Gene namesi
Name:Uba5
Synonyms:Ube1dc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1913913 Uba5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death at embryonic day 12.5.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949711 – 403Ubiquitin-like modifier-activating enzyme 5Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei357PhosphoserineBy similarity1
Modified residuei392PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VE47
MaxQBiQ8VE47
PaxDbiQ8VE47
PeptideAtlasiQ8VE47
PRIDEiQ8VE47

PTM databases

iPTMnetiQ8VE47
PhosphoSitePlusiQ8VE47
SwissPalmiQ8VE47

Expressioni

Gene expression databases

BgeeiENSMUSG00000032557 Expressed in 295 organ(s), highest expression level in lacrimal gland
CleanExiMM_UBA5
ExpressionAtlasiQ8VE47 baseline and differential
GenevisibleiQ8VE47 MM

Interactioni

Subunit structurei

Interacts (via C-terminus) with UFC1. Interacts with UFM1.By similarity

Protein-protein interaction databases

BioGridi211631, 1 interactor
IntActiQ8VE47, 3 interactors
MINTiQ8VE47
STRINGi10090.ENSMUSP00000035166

Structurei

3D structure databases

ProteinModelPortaliQ8VE47
SMRiQ8VE47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.Sequence analysis

Phylogenomic databases

eggNOGiKOG2336 Eukaryota
COG0476 LUCA
GeneTreeiENSGT00930000150982
HOGENOMiHOG000256352
HOVERGENiHBG056496
InParanoidiQ8VE47
KOiK12164
OMAiETHNYNI
OrthoDBiEOG091G0BHP
PhylomeDBiQ8VE47
TreeFamiTF314168

Family and domain databases

InterProiView protein in InterPro
IPR029752 D-isomer_DH_CS1
IPR000594 ThiF_NAD_FAD-bd
IPR035985 Ubiquitin-activating_enz
PfamiView protein in Pfam
PF00899 ThiF, 1 hit
SUPFAMiSSF69572 SSF69572, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q8VE47-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADSVERLRQ RVEELEQELA RERTRRSGGD GHCGRTRIQE MSDEVLDSNP
60 70 80 90 100
YSRLMALKRM GIVSDYKKIR TYAVAIVGVG GVGSVTAEML TRCGIGKLLL
110 120 130 140 150
FDYDKVELAN MNRLFFQPYQ AGLSKVHAAE HTLRNINPDV LFEVHNYNIT
160 170 180 190 200
TVEHFEHFMN RISNGGLEEG QPVDLVLSCV DNFEARMAIN TACNELGQTW
210 220 230 240 250
MESGVSENAV SGHIQLMIPG ESACFACAPP LVVASNIDEK TLKREGVCAA
260 270 280 290 300
SLPTTMGVVA GILVQNVLKF LLKFGTVSFY LGYNAMQDFF PTMFMKPNPQ
310 320 330 340 350
CDDKNCRKQQ EEYKKRAAAL PTQEAEPQEE AEVVHEDNEW GIELVSEVSE
360 370 380 390 400
EELKNSSGPV PTLPEGITVA YTVPKKTEDS ASEVTVEDSG ESLEDLMARM

KNM
Length:403
Mass (Da):44,790
Last modified:March 2, 2010 - v2
Checksum:i6C022AF9E0B7C83B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RH76D6RH76_MOUSE
Ubiquitin-like modifier-activating ...
Uba5
130Annotation score:
F6VRI6F6VRI6_MOUSE
Ubiquitin-like modifier-activating ...
Uba5
99Annotation score:
A0A1L1SSH6A0A1L1SSH6_MOUSE
Ubiquitin-like modifier-activating ...
Uba5
117Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11QR → HG in BAB30933 (PubMed:16141072).Curated2
Sequence conflicti30D → G in BAE30888 (PubMed:16141072).Curated1
Sequence conflicti153E → G in BAB30933 (PubMed:16141072).Curated1
Sequence conflicti156E → G in BAB30933 (PubMed:16141072).Curated1
Sequence conflicti156E → K in BAE30720 (PubMed:16141072).Curated1
Sequence conflicti156E → K in BAE30888 (PubMed:16141072).Curated1
Sequence conflicti164N → K in BAB30933 (PubMed:16141072).Curated1
Sequence conflicti224C → L in BAB30933 (PubMed:16141072).Curated1
Sequence conflicti300Q → H in AAH19764 (PubMed:15489334).Curated1
Sequence conflicti402N → T in AAH19764 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017787 mRNA Translation: BAB30933.1
AK151823 mRNA Translation: BAE30720.1
AK152029 mRNA Translation: BAE30888.1
AK168907 mRNA Translation: BAE40721.1
CH466560 Genomic DNA Translation: EDL21084.1
BC019764 mRNA Translation: AAH19764.1
CCDSiCCDS23457.1
RefSeqiNP_079968.2, NM_025692.3
UniGeneiMm.224935
Mm.479441

Genome annotation databases

EnsembliENSMUST00000035166; ENSMUSP00000035166; ENSMUSG00000032557
GeneIDi66663
KEGGimmu:66663
UCSCiuc009rhf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017787 mRNA Translation: BAB30933.1
AK151823 mRNA Translation: BAE30720.1
AK152029 mRNA Translation: BAE30888.1
AK168907 mRNA Translation: BAE40721.1
CH466560 Genomic DNA Translation: EDL21084.1
BC019764 mRNA Translation: AAH19764.1
CCDSiCCDS23457.1
RefSeqiNP_079968.2, NM_025692.3
UniGeneiMm.224935
Mm.479441

3D structure databases

ProteinModelPortaliQ8VE47
SMRiQ8VE47
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211631, 1 interactor
IntActiQ8VE47, 3 interactors
MINTiQ8VE47
STRINGi10090.ENSMUSP00000035166

PTM databases

iPTMnetiQ8VE47
PhosphoSitePlusiQ8VE47
SwissPalmiQ8VE47

Proteomic databases

EPDiQ8VE47
MaxQBiQ8VE47
PaxDbiQ8VE47
PeptideAtlasiQ8VE47
PRIDEiQ8VE47

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035166; ENSMUSP00000035166; ENSMUSG00000032557
GeneIDi66663
KEGGimmu:66663
UCSCiuc009rhf.2 mouse

Organism-specific databases

CTDi79876
MGIiMGI:1913913 Uba5

Phylogenomic databases

eggNOGiKOG2336 Eukaryota
COG0476 LUCA
GeneTreeiENSGT00930000150982
HOGENOMiHOG000256352
HOVERGENiHBG056496
InParanoidiQ8VE47
KOiK12164
OMAiETHNYNI
OrthoDBiEOG091G0BHP
PhylomeDBiQ8VE47
TreeFamiTF314168

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q8VE47
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032557 Expressed in 295 organ(s), highest expression level in lacrimal gland
CleanExiMM_UBA5
ExpressionAtlasiQ8VE47 baseline and differential
GenevisibleiQ8VE47 MM

Family and domain databases

InterProiView protein in InterPro
IPR029752 D-isomer_DH_CS1
IPR000594 ThiF_NAD_FAD-bd
IPR035985 Ubiquitin-activating_enz
PfamiView protein in Pfam
PF00899 ThiF, 1 hit
SUPFAMiSSF69572 SSF69572, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBA5_MOUSE
AccessioniPrimary (citable) accession number: Q8VE47
Secondary accession number(s): Q3TG27
, Q3U8X9, Q3U9E7, Q9CYD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 2, 2010
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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