UniProtKB - Q8VE37 (RCC1_MOUSE)
Protein
Regulator of chromosome condensation
Gene
Rcc1
Organism
Mus musculus (Mouse)
Status
Functioni
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.By similarity
GO - Molecular functioni
- chromatin binding Source: MGI
- histone binding Source: MGI
- nucleosomal DNA binding Source: UniProtKB
- nucleosome binding Source: MGI
- protein heterodimerization activity Source: MGI
- Ran GTPase binding Source: MGI
- Ran guanyl-nucleotide exchange factor activity Source: MGI
- sulfate binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB
- G1/S transition of mitotic cell cycle Source: MGI
- mitotic spindle organization Source: MGI
- Ran protein signal transduction Source: GO_Central
- regulation of mitotic nuclear division Source: MGI
- regulation of mitotic spindle assembly Source: GO_Central
- spindle assembly Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Guanine-nucleotide releasing factor |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-9615933, Postmitotic nuclear pore complex (NPC) reformation |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of chromosome condensationAlternative name(s): Chromosome condensation protein 1 |
Gene namesi | Name:Rcc1 Synonyms:Chc1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913989, Rcc1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
- Cytoplasm By similarity
Note: Predominantly nuclear in interphase cells. Binds to mitotic chromosomes.By similarity
Nucleus
- condensed nuclear chromosome Source: MGI
- nuclear chromatin Source: MGI
- nuclear membrane Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- chromatin Source: MGI
- cytoplasm Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Chromosome, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000206630 | 2 – 421 | Regulator of chromosome condensationAdd BLAST | 420 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N,N-dimethylproline; alternate1 Publication | 1 | |
Modified residuei | 2 | N-methylproline; alternate1 Publication | 1 | |
Modified residuei | 11 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Dimethylation produces a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.By similarity
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q8VE37 |
MaxQBi | Q8VE37 |
PaxDbi | Q8VE37 |
PRIDEi | Q8VE37 |
PTM databases
iPTMneti | Q8VE37 |
PhosphoSitePlusi | Q8VE37 |
SwissPalmi | Q8VE37 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028896, Expressed in ectoplacental cone and 271 other tissues |
ExpressionAtlasi | Q8VE37, baseline and differential |
Genevisiblei | Q8VE37, MM |
Interactioni
Subunit structurei
Interacts with RAN.
Interacts (via N-terminus and RCC1 repeats) with KPNA4.
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.
By similarityGO - Molecular functioni
- histone binding Source: MGI
- protein heterodimerization activity Source: MGI
- Ran GTPase binding Source: MGI
- Ran guanyl-nucleotide exchange factor activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 221378, 76 interactors |
IntActi | Q8VE37, 64 interactors |
MINTi | Q8VE37 |
STRINGi | 10090.ENSMUSP00000081271 |
Miscellaneous databases
RNActi | Q8VE37, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 34 – 84 | RCC1 1Add BLAST | 51 | |
Repeati | 85 – 136 | RCC1 2Add BLAST | 52 | |
Repeati | 138 – 189 | RCC1 3Add BLAST | 52 | |
Repeati | 191 – 257 | RCC1 4Add BLAST | 67 | |
Repeati | 258 – 311 | RCC1 5Add BLAST | 54 | |
Repeati | 312 – 362 | RCC1 6Add BLAST | 51 | |
Repeati | 363 – 416 | RCC1 7Add BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 4 – 24 | Bipartite nuclear localization signalBy similarityAdd BLAST | 21 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1426, Eukaryota |
GeneTreei | ENSGT00940000155543 |
HOGENOMi | CLU_005210_6_2_1 |
InParanoidi | Q8VE37 |
OMAi | WGNGQQY |
PhylomeDBi | Q8VE37 |
TreeFami | TF101139 |
Family and domain databases
Gene3Di | 2.130.10.30, 1 hit |
InterProi | View protein in InterPro IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens |
Pfami | View protein in Pfam PF00415, RCC1, 7 hits |
PRINTSi | PR00633, RCCNDNSATION |
SUPFAMi | SSF50985, SSF50985, 1 hit |
PROSITEi | View protein in PROSITE PS00625, RCC1_1, 1 hit PS00626, RCC1_2, 4 hits PS50012, RCC1_3, 7 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8VE37-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPKRIAKRR SPPEDAIPKS KKVKVSHRSH NTEPGLVLTL GQGDVGQLGL
60 70 80 90 100
GESVLERKKP ALVPLLQDVV QAEAGGMHTV CLSQSGQVYS FGCNDEGALG
110 120 130 140 150
RDTSVEGSEM VPGKVELQEK VVQVSAGDSH TAALTEDGRV FLWGSFRDNN
160 170 180 190 200
GVIGLLEPMK KSMVPVQVQL DAPVVKVASG NDHLVMLTND GDLYTLGCGE
210 220 230 240 250
QGQLGRVPEL FANRGGRQGL GRLLVPRCVL LKSRGTRGRV RFQDAFCGAY
260 270 280 290 300
FTFAISREGH VYGFGLSNYH QLGTPGTGSC FIPQNLTSFK NSTKSWVGFS
310 320 330 340 350
GGQHHTVCMD SEGKAYSLGR AEYGRLGLGE GAEEKSIPTL ISRLPVVSSV
360 370 380 390 400
ACGASVGYAV SKDGRVFAWG MGTNYQLGTG QDEDAWSPVE MTGKQLENRV
410 420
VLTVSSGGQH TVLLVKDQAQ S
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ6PFB2 | Q6PFB2_MOUSE | Rcc1 protein | Rcc1 Chc1 | 434 | Annotation score: | ||
A2CER2 | A2CER2_MOUSE | Regulator of chromosome condensatio... | Rcc1 | 116 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 45 | V → M in BAE29345 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK150153 mRNA Translation: BAE29345.1 BC019807 mRNA Translation: AAH19807.1 |
CCDSi | CCDS18723.1 |
RefSeqi | NP_598639.1, NM_133878.3 |
Genome annotation databases
Ensembli | ENSMUST00000084250; ENSMUSP00000081271; ENSMUSG00000028896 ENSMUST00000105951; ENSMUSP00000101571; ENSMUSG00000028896 |
GeneIDi | 100088 |
KEGGi | mmu:100088 |
UCSCi | uc008vbc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK150153 mRNA Translation: BAE29345.1 BC019807 mRNA Translation: AAH19807.1 |
CCDSi | CCDS18723.1 |
RefSeqi | NP_598639.1, NM_133878.3 |
3D structure databases
SMRi | Q8VE37 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221378, 76 interactors |
IntActi | Q8VE37, 64 interactors |
MINTi | Q8VE37 |
STRINGi | 10090.ENSMUSP00000081271 |
PTM databases
iPTMneti | Q8VE37 |
PhosphoSitePlusi | Q8VE37 |
SwissPalmi | Q8VE37 |
Proteomic databases
EPDi | Q8VE37 |
MaxQBi | Q8VE37 |
PaxDbi | Q8VE37 |
PRIDEi | Q8VE37 |
Protocols and materials databases
Antibodypediai | 4212, 402 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000084250; ENSMUSP00000081271; ENSMUSG00000028896 ENSMUST00000105951; ENSMUSP00000101571; ENSMUSG00000028896 |
GeneIDi | 100088 |
KEGGi | mmu:100088 |
UCSCi | uc008vbc.2, mouse |
Organism-specific databases
CTDi | 1104 |
MGIi | MGI:1913989, Rcc1 |
Phylogenomic databases
eggNOGi | KOG1426, Eukaryota |
GeneTreei | ENSGT00940000155543 |
HOGENOMi | CLU_005210_6_2_1 |
InParanoidi | Q8VE37 |
OMAi | WGNGQQY |
PhylomeDBi | Q8VE37 |
TreeFami | TF101139 |
Enzyme and pathway databases
Reactomei | R-MMU-9615933, Postmitotic nuclear pore complex (NPC) reformation |
Miscellaneous databases
BioGRID-ORCSi | 100088, 8 hits in 21 CRISPR screens |
ChiTaRSi | Rcc1, mouse |
PROi | PR:Q8VE37 |
RNActi | Q8VE37, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028896, Expressed in ectoplacental cone and 271 other tissues |
ExpressionAtlasi | Q8VE37, baseline and differential |
Genevisiblei | Q8VE37, MM |
Family and domain databases
Gene3Di | 2.130.10.30, 1 hit |
InterProi | View protein in InterPro IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens |
Pfami | View protein in Pfam PF00415, RCC1, 7 hits |
PRINTSi | PR00633, RCCNDNSATION |
SUPFAMi | SSF50985, SSF50985, 1 hit |
PROSITEi | View protein in PROSITE PS00625, RCC1_1, 1 hit PS00626, RCC1_2, 4 hits PS50012, RCC1_3, 7 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RCC1_MOUSE | |
Accessioni | Q8VE37Primary (citable) accession number: Q8VE37 Secondary accession number(s): Q3UDB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot