UniProtKB - Q8VDZ4 (ZDHC5_MOUSE)
Protein
Palmitoyltransferase ZDHHC5
Gene
Zdhhc5
Organism
Mus musculus (Mouse)
Status
Functioni
Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and is involved in a variety of cellular processes (PubMed:21820437, PubMed:22081607). Palmitoylates the G-protein coupled receptor SSTR5 and for FLOT2 (PubMed:21820437, PubMed:22081607).2 Publications
Miscellaneous
In neural stem cells, rapidly degraded through the proteasome pathway following growth factors withdrawal, a strategy used to induce differentiation.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 134 | S-palmitoyl cysteine intermediate1 Publication | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: MGI
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- positive regulation of pattern recognition receptor signaling pathway Source: UniProtKB
- positive regulation of protein localization to phagocytic vesicle Source: UniProtKB
- positive regulation of protein localization to plasma membrane Source: UniProtKB
- protein palmitoylation Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.225, 3474 |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase ZDHHC5 (EC:2.3.1.2251 Publication)Alternative name(s): Zinc finger DHHC domain-containing protein 5 Short name: DHHC-5 |
Gene namesi | Name:Zdhhc5 Synonyms:Kiaa1748 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923573, Zdhhc5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- dendrite Source: MGI
- integral component of membrane Source: UniProtKB-KW
- phagocytic vesicle Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 13 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 35 – 52 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 74 – 148 | CytoplasmicSequence analysisAdd BLAST | 75 | |
Transmembranei | 149 – 169 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 170 – 191 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 192 – 212 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 213 – 715 | CytoplasmicSequence analysisAdd BLAST | 503 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 134 | C → S: Loss of palmitoyltransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212869 | 1 – 715 | Palmitoyltransferase ZDHHC5Add BLAST | 715 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 247 | PhosphoserineBy similarity | 1 | |
Modified residuei | 294 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 296 | PhosphoserineCombined sources | 1 | |
Modified residuei | 299 | PhosphoserineCombined sources | 1 | |
Modified residuei | 303 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 345 | PhosphoserineCombined sources | 1 | |
Modified residuei | 348 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 350 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 380 | PhosphoserineCombined sources | 1 | |
Modified residuei | 398 | PhosphoserineBy similarity | 1 | |
Modified residuei | 406 | PhosphoserineBy similarity | 1 | |
Modified residuei | 409 | PhosphoserineBy similarity | 1 | |
Modified residuei | 411 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 415 | PhosphoserineBy similarity | 1 | |
Modified residuei | 425 | PhosphoserineBy similarity | 1 | |
Modified residuei | 429 | PhosphoserineCombined sources | 1 | |
Modified residuei | 432 | PhosphoserineCombined sources | 1 | |
Modified residuei | 436 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 529 | PhosphoserineBy similarity | 1 | |
Modified residuei | 554 | PhosphoserineBy similarity | 1 | |
Modified residuei | 617 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 621 | PhosphoserineCombined sources | 1 | |
Modified residuei | 659 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 684 | PhosphoserineBy similarity | 1 | |
Modified residuei | 694 | PhosphoserineBy similarity | 1 | |
Modified residuei | 697 | Omega-N-methylarginineCombined sources | 1 |
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PhosphoproteinProteomic databases
EPDi | Q8VDZ4 |
jPOSTi | Q8VDZ4 |
PaxDbi | Q8VDZ4 |
PeptideAtlasi | Q8VDZ4 |
PRIDEi | Q8VDZ4 |
ProteomicsDBi | 275140 [Q8VDZ4-1] 275141 [Q8VDZ4-2] |
PTM databases
iPTMneti | Q8VDZ4 |
PhosphoSitePlusi | Q8VDZ4 |
SwissPalmi | Q8VDZ4 |
Expressioni
Inductioni
In neural stem cells, rapid up-regulation by EGF, combined with FGF2 and heparin.
Gene expression databases
Bgeei | ENSMUSG00000034075, Expressed in cornea and 303 other tissues |
Genevisiblei | Q8VDZ4, MM |
Interactioni
Binary interactionsi
Q8VDZ4
With | #Exp. | IntAct |
---|---|---|
GOLGA7B [Q9D428] | 2 | EBI-7057556,EBI-25635843 |
Protein-protein interaction databases
BioGRIDi | 230716, 2 interactors |
IntActi | Q8VDZ4, 3 interactors |
MINTi | Q8VDZ4 |
STRINGi | 10090.ENSMUSP00000048198 |
Miscellaneous databases
RNActi | Q8VDZ4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 154 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.By similarity
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156001 |
HOGENOMi | CLU_013779_0_0_1 |
InParanoidi | Q8VDZ4 |
OMAi | TLANFCM |
OrthoDBi | 1264614at2759 |
PhylomeDBi | Q8VDZ4 |
TreeFami | TF354263 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8VDZ4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPAESGKRFK PSKYVPVSAA AIFLVGATTL FFAFTCPGLS LNVSPAVPIY
60 70 80 90 100
NAIMFLFVLA NFSMATFMDP GIFPRAEEDE DKEDDFRAPL YKTVEIKGIQ
110 120 130 140 150
VRMKWCATCR FYRPPRCSHC SVCDNCVEEF DHHCPWVNNC IGRRNYRYFF
160 170 180 190 200
LFLLSLTAHI MGVFGFGLLY VLYHIEELSG VRTAVTMAVM CVAGLFFIPV
210 220 230 240 250
AGLTGFHVVL VARGRTTNEQ VTGKFRGGVN PFTNGCCNNV SRVLCSSPAP
260 270 280 290 300
RYLGRPKKEK TIVIRPPFLR PEVSDGQITV KIMDNGIQGE LRRTKSKGSL
310 320 330 340 350
EITESQSADA EPPPPPKPDL SRYTGLRTHL SLATNEDSSL LGKDSPPTPT
360 370 380 390 400
MYKYRPGYSS SSTSAAMPHS SSAKLSRGDS LKEPTSIADS SRHPSYRSEP
410 420 430 440 450
SLEPESFRSP TFGKSFHFDP LSSGSRSSSL KSAQGTGFEL GQLQSIRSEG
460 470 480 490 500
TTSTSYKSLA NQTRNGSLSY DSLLTPSDSP DFESVQAGPE PDPPLGYTSP
510 520 530 540 550
FLSARLAQQR EAERHPRLLP TGPPHREPSP VRYDNLSRHI VASLQEREKL
560 570 580 590 600
LRQSPPLAGR EEEPGLGDSG IQSTPGSGHA PRTSSSSDDS KRSPLSKTPL
610 620 630 640 650
GRPAVPRFGK PDGLRSRGLG SPEPGTTAPY LGRSISYSSQ KAPSGVSETE
660 670 680 690 700
EVALQPLLTP KDEVQLKTTY SKSNGQPKSI GSASPGPGQP PLSSPTRGGV
710
KKVSGVGGTT YEISV
Sequence cautioni
The sequence BAD32529 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006936 | 641 – 652 | KAPSG…ETEEV → NPHLVSQRQRK in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_006937 | 653 – 715 | Missing in isoform 2. 1 PublicationAdd BLAST | 63 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY894891 mRNA Translation: AAX73370.1 AK082513 mRNA Translation: BAC38513.1 AK075641 mRNA Translation: BAC35875.1 AK173251 mRNA Translation: BAD32529.1 Different initiation. BC020051 mRNA Translation: AAH20051.1 BC027047 mRNA Translation: AAH27047.1 BC065155 mRNA Translation: AAH65155.1 |
CCDSi | CCDS16190.1 [Q8VDZ4-1] |
RefSeqi | NP_659136.1, NM_144887.4 [Q8VDZ4-1] |
Genome annotation databases
Ensembli | ENSMUST00000035840; ENSMUSP00000048198; ENSMUSG00000034075 [Q8VDZ4-1] |
GeneIDi | 228136 |
KEGGi | mmu:228136 |
UCSCi | uc008kiz.2, mouse [Q8VDZ4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY894891 mRNA Translation: AAX73370.1 AK082513 mRNA Translation: BAC38513.1 AK075641 mRNA Translation: BAC35875.1 AK173251 mRNA Translation: BAD32529.1 Different initiation. BC020051 mRNA Translation: AAH20051.1 BC027047 mRNA Translation: AAH27047.1 BC065155 mRNA Translation: AAH65155.1 |
CCDSi | CCDS16190.1 [Q8VDZ4-1] |
RefSeqi | NP_659136.1, NM_144887.4 [Q8VDZ4-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 230716, 2 interactors |
IntActi | Q8VDZ4, 3 interactors |
MINTi | Q8VDZ4 |
STRINGi | 10090.ENSMUSP00000048198 |
PTM databases
iPTMneti | Q8VDZ4 |
PhosphoSitePlusi | Q8VDZ4 |
SwissPalmi | Q8VDZ4 |
Proteomic databases
EPDi | Q8VDZ4 |
jPOSTi | Q8VDZ4 |
PaxDbi | Q8VDZ4 |
PeptideAtlasi | Q8VDZ4 |
PRIDEi | Q8VDZ4 |
ProteomicsDBi | 275140 [Q8VDZ4-1] 275141 [Q8VDZ4-2] |
Protocols and materials databases
Antibodypediai | 3027, 90 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000035840; ENSMUSP00000048198; ENSMUSG00000034075 [Q8VDZ4-1] |
GeneIDi | 228136 |
KEGGi | mmu:228136 |
UCSCi | uc008kiz.2, mouse [Q8VDZ4-1] |
Organism-specific databases
CTDi | 25921 |
MGIi | MGI:1923573, Zdhhc5 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156001 |
HOGENOMi | CLU_013779_0_0_1 |
InParanoidi | Q8VDZ4 |
OMAi | TLANFCM |
OrthoDBi | 1264614at2759 |
PhylomeDBi | Q8VDZ4 |
TreeFami | TF354263 |
Enzyme and pathway databases
BRENDAi | 2.3.1.225, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 228136, 7 hits in 53 CRISPR screens |
ChiTaRSi | Zdhhc5, mouse |
PROi | PR:Q8VDZ4 |
RNActi | Q8VDZ4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034075, Expressed in cornea and 303 other tissues |
Genevisiblei | Q8VDZ4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDHC5_MOUSE | |
Accessioni | Q8VDZ4Primary (citable) accession number: Q8VDZ4 Secondary accession number(s): Q2TGE8, Q69ZB5, Q8R2X7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families