UniProtKB - Q8VDL9 (GLIS2_MOUSE)
Zinc finger protein GLIS2
Glis2
Functioni
Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.
6 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 168 – 193 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 202 – 229 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 235 – 257 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 263 – 287 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 293 – 317 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: BHF-UCL
GO - Biological processi
- cell differentiation involved in kidney development Source: MGI
- central nervous system development Source: GO_Central
- hematopoietic stem cell homeostasis Source: MGI
- kidney development Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
- negative regulation of smoothened signaling pathway Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of protein localization to nucleus Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription from RNA polymerase II promoter involved in kidney development Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein GLIS2Alternative name(s): GLI-similar 2 Neuronal Krueppel-like protein Zinc finger protein GLI5 |
Gene namesi | Name:Glis2 Synonyms:Gli5, Nkl |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1932535, Glis2 |
VEuPathDBi | HostDB:ENSMUSG00000014303 |
Subcellular locationi
Nucleus
Cytoplasm and Cytosol
Nucleus
- nuclear speck Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: UniProtKB-SubCell
- non-motile cilium Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 170 | C → A: Impairs DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 175 | C → A: Abolishes interaction with CTNNB1. No effect on nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 288 | Y → A: No effect on C-terminus cleavage. 1 Publication | 1 | |
Mutagenesisi | 290 | D → A: Impairs C-terminus cleavage. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000286984 | 1 – 521 | Zinc finger protein GLIS2Add BLAST | 521 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 287 – 288 | Cleavage | 2 |
Proteomic databases
PaxDbi | Q8VDL9 |
PRIDEi | Q8VDL9 |
ProteomicsDBi | 267453 |
PTM databases
iPTMneti | Q8VDL9 |
PhosphoSitePlusi | Q8VDL9 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000014303, Expressed in metanephric collecting duct and 245 other tissues |
ExpressionAtlasi | Q8VDL9, baseline and differential |
Genevisiblei | Q8VDL9, MM |
Interactioni
Subunit structurei
Interacts with CTBP1 and HDAC3.
Interacts with CTNNB1 and CTNND1.
Interacts with SUFU.
4 PublicationsProtein-protein interaction databases
BioGRIDi | 219913, 2 interactors |
CORUMi | Q8VDL9 |
STRINGi | 10090.ENSMUSP00000014447 |
Miscellaneous databases
RNActi | Q8VDL9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 174 | Interaction with CTNND11 PublicationAdd BLAST | 140 | |
Regioni | 41 – 63 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 71 – 137 | Transcription activationAdd BLAST | 67 | |
Regioni | 84 – 110 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 148 – 171 | Transcription repressionAdd BLAST | 24 | |
Regioni | 436 – 501 | DisorderedSequence analysisAdd BLAST | 66 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 86 – 103 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 457 – 472 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 168 – 193 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 202 – 229 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 235 – 257 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 263 – 287 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 293 – 317 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158383 |
HOGENOMi | CLU_031801_1_0_1 |
InParanoidi | Q8VDL9 |
OMAi | APKDKCL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8VDL9 |
TreeFami | TF351425 |
Family and domain databases
InterProi | View protein in InterPro IPR030432, Glis2 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR19818:SF84, PTHR19818:SF84, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MHSLDEPLDL KLSITKLRAA REKRERTLGV VRHHALHREL GLVDDSPAPG
60 70 80 90 100
SPGSPPPGFL LNPKFPEKVD GRFSAAPLVD LSLSPPSGLD SPNGSSSLSP
110 120 130 140 150
ECQGNGDLPP LPTAVDFQPL RYLDGVPSSF QFFLPLGSGG ALHLPASSFL
160 170 180 190 200
PPPKDKCLSP ELPLAKQLVC RWAKCNQLFE LLQDLVDHVN DHHVKPEQDA
210 220 230 240 250
RYCCHWEGCA RHGRGFNARY KMLIHIRTHT NEKPHRCPTC NKSFSRLENL
260 270 280 290 300
KIHNRSHTGE KPYVCPYEGC NKRYSNSSDR FKHTRTHYVD KPYYCKMPGC
310 320 330 340 350
HKRYTDPSSL RKHIKAHGHF VSHEQQELLQ LRPPPKPPLP TPDSGSYVSG
360 370 380 390 400
AQIIIPNPAA LFGGPSLPGL PLPLPPGPLD LSALACGNGG GGGGGIGPGL
410 420 430 440 450
PGSVLPLNLA KNPLLPSPFG AGGLGLPVVS LLGGSAGSKA EGEKGRGSVP
460 470 480 490 500
ARVLGLEDHK TPLERTERSR SRPSPDGLPL LPGTVLDLST GNSAASSPEV
510 520
LTPGWVVIPP GSVLLKPAVV N
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YYX4 | D3YYX4_MOUSE | Zinc finger protein GLIS2 | Glis2 | 109 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 – 88 | SG → CE in AAL93213 (PubMed:11741991).Curated | 2 | |
Sequence conflicti | 208 | G → D in AAK00953 (PubMed:11741991).Curated | 1 | |
Sequence conflicti | 210 | Missing in AAK28410 (PubMed:11262234).Curated | 1 | |
Sequence conflicti | 249 | N → K in AAL93213 (PubMed:11741991).Curated | 1 | |
Sequence conflicti | 315 – 318 | KAHG → RPW in AAL93213 (PubMed:11741991).Curated | 4 | |
Sequence conflicti | 387 | G → A in AAK28410 (PubMed:11262234).Curated | 1 | |
Sequence conflicti | 458 | D → N in AAL93213 (PubMed:11741991).Curated | 1 | |
Sequence conflicti | 458 | D → N in AAK00953 (PubMed:11741991).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF249340 mRNA Translation: AAK28410.1 AF325913 mRNA Translation: AAK00953.1 AF336135 Genomic DNA Translation: AAL93213.1 BC021517 mRNA Translation: AAH21517.1 |
CCDSi | CCDS27920.1 |
RefSeqi | NP_112461.2, NM_031184.3 XP_006522831.1, XM_006522768.3 |
Genome annotation databases
Ensembli | ENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303 |
GeneIDi | 83396 |
KEGGi | mmu:83396 |
UCSCi | uc007xzw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF249340 mRNA Translation: AAK28410.1 AF325913 mRNA Translation: AAK00953.1 AF336135 Genomic DNA Translation: AAL93213.1 BC021517 mRNA Translation: AAH21517.1 |
CCDSi | CCDS27920.1 |
RefSeqi | NP_112461.2, NM_031184.3 XP_006522831.1, XM_006522768.3 |
3D structure databases
SMRi | Q8VDL9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 219913, 2 interactors |
CORUMi | Q8VDL9 |
STRINGi | 10090.ENSMUSP00000014447 |
PTM databases
iPTMneti | Q8VDL9 |
PhosphoSitePlusi | Q8VDL9 |
Proteomic databases
PaxDbi | Q8VDL9 |
PRIDEi | Q8VDL9 |
ProteomicsDBi | 267453 |
Protocols and materials databases
Antibodypediai | 11033, 121 antibodies from 23 providers |
DNASUi | 83396 |
Genome annotation databases
Ensembli | ENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303 |
GeneIDi | 83396 |
KEGGi | mmu:83396 |
UCSCi | uc007xzw.1, mouse |
Organism-specific databases
CTDi | 84662 |
MGIi | MGI:1932535, Glis2 |
VEuPathDBi | HostDB:ENSMUSG00000014303 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000158383 |
HOGENOMi | CLU_031801_1_0_1 |
InParanoidi | Q8VDL9 |
OMAi | APKDKCL |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8VDL9 |
TreeFami | TF351425 |
Miscellaneous databases
BioGRID-ORCSi | 83396, 1 hit in 63 CRISPR screens |
ChiTaRSi | Glis1, mouse |
PROi | PR:Q8VDL9 |
RNActi | Q8VDL9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014303, Expressed in metanephric collecting duct and 245 other tissues |
ExpressionAtlasi | Q8VDL9, baseline and differential |
Genevisiblei | Q8VDL9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR030432, Glis2 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR19818:SF84, PTHR19818:SF84, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GLIS2_MOUSE | |
Accessioni | Q8VDL9Primary (citable) accession number: Q8VDL9 Secondary accession number(s): Q8R4X9, Q99MY6, Q99P73 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families