Glis2

Functionⁱ

Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.6 Publications

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	168 – 193	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	26
Zinc fingerⁱ	202 – 229	C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST	28
Zinc fingerⁱ	235 – 257	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	263 – 287	C2H2-type 4PROSITE-ProRule annotationAdd BLAST	25
Zinc fingerⁱ	293 – 317	C2H2-type 5PROSITE-ProRule annotationAdd BLAST	25

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 11262234
- 17344476
DNA-binding transcription factor activity
Source: MGI
Inferred from sequence or structural similarityⁱ
- 11262234
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
metal ion binding Source: UniProtKB-KW
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific
Source: BHF-UCL
Inferred from direct assayⁱ
- 21127075
RNA polymerase II regulatory region sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 18298960
transcription regulatory region DNA binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 21127075

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation involved in kidney development
Source: MGI
Inferred from mutant phenotypeⁱ
- 17618285
kidney development
Source: MGI
Inferred from mutant phenotypeⁱ
- 18227149
negative regulation of DNA-binding transcription factor activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 21127075
negative regulation of smoothened signaling pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21816948
negative regulation of transcription, DNA-templated
Source: UniProtKB
Inferred from direct assayⁱ
- 11741991
negative regulation of transcription by RNA polymerase II
Source: BHF-UCL
Inferred from direct assayⁱ
- 21127075
nervous system development Source: UniProtKB-KW
positive regulation of protein localization to nucleus
Source: MGI
Inferred from genetic interactionⁱ
- 17344476
positive regulation of transcription, DNA-templated
Source: UniProtKB
Inferred from direct assayⁱ
- 11741991
positive regulation of transcription by RNA polymerase II
Source: BHF-UCL
Inferred from direct assayⁱ
- 21127075
regulation of transcription by RNA polymerase II
Source: MGI
Inferred from sequence or structural similarityⁱ
- 11262234
regulation of transcription from RNA polymerase II promoter involved in kidney development
Source: MGI
Inferred from mutant phenotypeⁱ
- 18227149

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Developmental protein, DNA-binding, Repressor
Biological process	Differentiation, Neurogenesis, Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Zinc finger protein GLIS2 Alternative name(s): GLI-similar 2 Neuronal Krueppel-like protein Zinc finger protein GLI5
Gene namesⁱ	Name:Glis2 Synonyms:Gli5, Nkl
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:1932535 Glis2

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	170	C → A: Impairs DNA-binding. 1 Publication	1
Mutagenesisⁱ	175	C → A: Abolishes interaction with CTNNB1. No effect on nuclear localization. 1 Publication	1
Mutagenesisⁱ	288	Y → A: No effect on C-terminus cleavage. 1 Publication	1
Mutagenesisⁱ	290	D → A: Impairs C-terminus cleavage. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000286984	1 – 521	Zinc finger protein GLIS2Add BLAST		521

Post-translational modificationⁱ

C-terminus cleavage is induced by interaction with CTNND1 and enhances by Src tyrosine kinase.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	287 – 288	Cleavage		2

Proteomic databases

PaxDbⁱ	Q8VDL9
PRIDEⁱ	Q8VDL9

PTM databases

iPTMnetⁱ	Q8VDL9
PhosphoSitePlusⁱ	Q8VDL9

Expressionⁱ

Tissue specificityⁱ

Expressed at high levels in kidney, and at lower levels in heart and lung.1 Publication

Developmental stageⁱ

Expression begins at E9.5 in cranial ganglia, dorsal root ganglia and neural tube. At E10.5, broadly expressed in the intermediate zone of the hindbrain, spinal cord and dorsal root ganglia. By E12.5, expression in the spinal cord becomes restricted to a narrow band of cells in the ventricular zone.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000014303 Expressed in 217 organ(s), highest expression level in metanephric collecting duct
CleanExⁱ	MM_GLIS2
ExpressionAtlasⁱ	Q8VDL9 baseline and differential
Genevisibleⁱ	Q8VDL9 MM

Interactionⁱ

Subunit structureⁱ

Interacts with CTBP1 and HDAC3. Interacts with CTNNB1 and CTNND1. Interacts with SUFU.4 Publications

Protein-protein interaction databases

BioGridⁱ	219913, 2 interactors
CORUMⁱ	Q8VDL9
STRINGⁱ	10090.ENSMUSP00000014447

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8VDL9
SMRⁱ	Q8VDL9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	35 – 174	Interaction with CTNND11 PublicationAdd BLAST	140
Regionⁱ	71 – 137	Transcription activationAdd BLAST	67
Regionⁱ	148 – 171	Transcription repressionAdd BLAST	24

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	333 – 378	Pro-richAdd BLAST		46
Compositional biasⁱ	387 – 447	Gly-richAdd BLAST		61

Domainⁱ

The C2H2-type zinc finger 1 has a major repressor function and is required for CTNNB1 binding.

Sequence similaritiesⁱ

Belongs to the GLI C2H2-type zinc-finger protein family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	168 – 193	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	26
Zinc fingerⁱ	202 – 229	C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST	28
Zinc fingerⁱ	235 – 257	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	263 – 287	C2H2-type 4PROSITE-ProRule annotationAdd BLAST	25
Zinc fingerⁱ	293 – 317	C2H2-type 5PROSITE-ProRule annotationAdd BLAST	25

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140802
HOGENOMⁱ	HOG000065778
HOVERGENⁱ	HBG101807
InParanoidⁱ	Q8VDL9
KEGG Orthology (KO) More...KOⁱ	K09233
OMAⁱ	DKCLSPE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0IZP
PhylomeDBⁱ	Q8VDL9
TreeFamⁱ	TF351425

Family and domain databases

InterProⁱ	View protein in InterPro IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00096 zf-C2H2, 2 hits
SMARTⁱ	View protein in SMART SM00355 ZnF_C2H2, 5 hits
SUPFAMⁱ	SSF57667 SSF57667, 3 hits
PROSITEⁱ	View protein in PROSITE PS00028 ZINC_FINGER_C2H2_1, 4 hits PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q8VDL9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MHSLDEPLDL KLSITKLRAA REKRERTLGV VRHHALHREL GLVDDSPAPG 
        60         70         80         90        100
SPGSPPPGFL LNPKFPEKVD GRFSAAPLVD LSLSPPSGLD SPNGSSSLSP 
       110        120        130        140        150
ECQGNGDLPP LPTAVDFQPL RYLDGVPSSF QFFLPLGSGG ALHLPASSFL 
       160        170        180        190        200
PPPKDKCLSP ELPLAKQLVC RWAKCNQLFE LLQDLVDHVN DHHVKPEQDA 
       210        220        230        240        250
RYCCHWEGCA RHGRGFNARY KMLIHIRTHT NEKPHRCPTC NKSFSRLENL 
       260        270        280        290        300
KIHNRSHTGE KPYVCPYEGC NKRYSNSSDR FKHTRTHYVD KPYYCKMPGC 
       310        320        330        340        350
HKRYTDPSSL RKHIKAHGHF VSHEQQELLQ LRPPPKPPLP TPDSGSYVSG 
       360        370        380        390        400
AQIIIPNPAA LFGGPSLPGL PLPLPPGPLD LSALACGNGG GGGGGIGPGL 
       410        420        430        440        450
PGSVLPLNLA KNPLLPSPFG AGGLGLPVVS LLGGSAGSKA EGEKGRGSVP 
       460        470        480        490        500
ARVLGLEDHK TPLERTERSR SRPSPDGLPL LPGTVLDLST GNSAASSPEV 
       510        520 
LTPGWVVIPP GSVLLKPAVV N

Length:521

Mass (Da):55,842

Last modified:March 1, 2002 - v1

Checksum:ⁱ6B5F78F4A742C72D

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	D3YYX4	D3YYX4_MOUSE		Zinc finger protein GLIS2 Zinc finger protein GLIS2	Glis2	109	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	87 – 88	SG → CE in AAL93213 (PubMed:11741991).Curated	2
Sequence conflictⁱ	208	G → D in AAK00953 (PubMed:11741991).Curated	1
Sequence conflictⁱ	210	Missing in AAK28410 (PubMed:11262234).Curated	1
Sequence conflictⁱ	249	N → K in AAL93213 (PubMed:11741991).Curated	1
Sequence conflictⁱ	315 – 318	KAHG → RPW in AAL93213 (PubMed:11741991).Curated	4
Sequence conflictⁱ	387	G → A in AAK28410 (PubMed:11262234).Curated	1
Sequence conflictⁱ	458	D → N in AAL93213 (PubMed:11741991).Curated	1
Sequence conflictⁱ	458	D → N in AAK00953 (PubMed:11741991).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF249340 mRNA Translation: AAK28410.1 AF325913 mRNA Translation: AAK00953.1 AF336135 Genomic DNA Translation: AAL93213.1 BC021517 mRNA Translation: AAH21517.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27920.1
NCBI Reference Sequences More...RefSeqⁱ	NP_112461.2, NM_031184.3 XP_006522831.1, XM_006522768.3
UniGeneⁱ	Mm.134072

Genome annotation databases

Ensemblⁱ	ENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303
GeneIDⁱ	83396
KEGGⁱ	mmu:83396
UCSC genome browser More...UCSCⁱ	uc007xzw.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8VDL9	Zinc finger protein GLIS2 (Fragment)		MOUSE		109	UniRef100_Q8VDL9

Protein	Similar proteins		Species	Score	Length	Source
Q8VDL9	Zinc finger protein GLIS2		HUMAN		524	UniRef90_Q8VDL9
Zinc finger protein GLIS2 (Fragment)		MOUSE		109
GLIS family zinc finger 2		CANLF		627
Zinc finger protein GLIS2		CRIGR		549
Uncharacterized protein		NEOLE		530
+114

Protein	Similar proteins		Species	Score	Length	Source
Q8VDL9	Zinc finger protein GLIS2		HUMAN		524	UniRef50_Q8VDL9
Zinc finger protein GLIS2 (Fragment)		MOUSE		109
GLIS family zinc finger 2		CANLF		627
Zinc finger protein GLIS2		CRIGR		549
Uncharacterized protein		NEOLE		530
+254

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF249340 mRNA Translation: AAK28410.1 AF325913 mRNA Translation: AAK00953.1 AF336135 Genomic DNA Translation: AAL93213.1 BC021517 mRNA Translation: AAH21517.1
CCDSⁱ	CCDS27920.1
RefSeqⁱ	NP_112461.2, NM_031184.3 XP_006522831.1, XM_006522768.3
UniGeneⁱ	Mm.134072

3D structure databases

ProteinModelPortalⁱ	Q8VDL9
SMRⁱ	Q8VDL9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	219913, 2 interactors
CORUMⁱ	Q8VDL9
STRINGⁱ	10090.ENSMUSP00000014447

PTM databases

iPTMnetⁱ	Q8VDL9
PhosphoSitePlusⁱ	Q8VDL9

Proteomic databases

PaxDbⁱ	Q8VDL9
PRIDEⁱ	Q8VDL9

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303
GeneIDⁱ	83396
KEGGⁱ	mmu:83396
UCSCⁱ	uc007xzw.1 mouse

Organism-specific databases

CTDⁱ	84662
MGIⁱ	MGI:1932535 Glis2

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
GeneTreeⁱ	ENSGT00900000140802
HOGENOMⁱ	HOG000065778
HOVERGENⁱ	HBG101807
InParanoidⁱ	Q8VDL9
KOⁱ	K09233
OMAⁱ	DKCLSPE
OrthoDBⁱ	EOG091G0IZP
PhylomeDBⁱ	Q8VDL9
TreeFamⁱ	TF351425

Miscellaneous databases

ChiTaRSⁱ	Glis1 mouse
Protein Ontology More...PROⁱ	PR:Q8VDL9
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000014303 Expressed in 217 organ(s), highest expression level in metanephric collecting duct
CleanExⁱ	MM_GLIS2
ExpressionAtlasⁱ	Q8VDL9 baseline and differential
Genevisibleⁱ	Q8VDL9 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfamⁱ	View protein in Pfam PF00096 zf-C2H2, 2 hits
SMARTⁱ	View protein in SMART SM00355 ZnF_C2H2, 5 hits
SUPFAMⁱ	SSF57667 SSF57667, 3 hits
PROSITEⁱ	View protein in PROSITE PS00028 ZINC_FINGER_C2H2_1, 4 hits PS50157 ZINC_FINGER_C2H2_2, 4 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLIS2_MOUSE
Accessionⁱ	Q8VDL9Primary (citable) accession number: Q8VDL9 Secondary accession number(s): Q8R4X9, Q99MY6, Q99P73
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
	Last sequence update:	March 1, 2002
	Last modified:	November 7, 2018
	This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8VDL9 (GLIS2_MOUSE)

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Zinc finger protein GLIS2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Sites

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ