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Protein

Zinc finger protein GLIS2

Gene

Glis2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri168 – 193C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri202 – 229C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri235 – 257C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri263 – 287C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri293 – 317C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding, Repressor
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein GLIS2
Alternative name(s):
GLI-similar 2
Neuronal Krueppel-like protein
Zinc finger protein GLI5
Gene namesi
Name:Glis2
Synonyms:Gli5, Nkl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1932535 Glis2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170C → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi175C → A: Abolishes interaction with CTNNB1. No effect on nuclear localization. 1 Publication1
Mutagenesisi288Y → A: No effect on C-terminus cleavage. 1 Publication1
Mutagenesisi290D → A: Impairs C-terminus cleavage. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002869841 – 521Zinc finger protein GLIS2Add BLAST521

Post-translational modificationi

C-terminus cleavage is induced by interaction with CTNND1 and enhances by Src tyrosine kinase.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei287 – 288Cleavage2

Proteomic databases

PaxDbiQ8VDL9
PRIDEiQ8VDL9

PTM databases

iPTMnetiQ8VDL9
PhosphoSitePlusiQ8VDL9

Expressioni

Tissue specificityi

Expressed at high levels in kidney, and at lower levels in heart and lung.1 Publication

Developmental stagei

Expression begins at E9.5 in cranial ganglia, dorsal root ganglia and neural tube. At E10.5, broadly expressed in the intermediate zone of the hindbrain, spinal cord and dorsal root ganglia. By E12.5, expression in the spinal cord becomes restricted to a narrow band of cells in the ventricular zone.1 Publication

Gene expression databases

BgeeiENSMUSG00000014303 Expressed in 217 organ(s), highest expression level in metanephric collecting duct
CleanExiMM_GLIS2
ExpressionAtlasiQ8VDL9 baseline and differential
GenevisibleiQ8VDL9 MM

Interactioni

Subunit structurei

Interacts with CTBP1 and HDAC3. Interacts with CTNNB1 and CTNND1. Interacts with SUFU.4 Publications

Protein-protein interaction databases

BioGridi219913, 2 interactors
CORUMiQ8VDL9
STRINGi10090.ENSMUSP00000014447

Structurei

3D structure databases

ProteinModelPortaliQ8VDL9
SMRiQ8VDL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 174Interaction with CTNND11 PublicationAdd BLAST140
Regioni71 – 137Transcription activationAdd BLAST67
Regioni148 – 171Transcription repressionAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 378Pro-richAdd BLAST46
Compositional biasi387 – 447Gly-richAdd BLAST61

Domaini

The C2H2-type zinc finger 1 has a major repressor function and is required for CTNNB1 binding.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri168 – 193C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri202 – 229C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri235 – 257C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri263 – 287C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri293 – 317C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00900000140802
HOGENOMiHOG000065778
HOVERGENiHBG101807
InParanoidiQ8VDL9
KOiK09233
OMAiDKCLSPE
OrthoDBiEOG091G0IZP
PhylomeDBiQ8VDL9
TreeFamiTF351425

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 2 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8VDL9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHSLDEPLDL KLSITKLRAA REKRERTLGV VRHHALHREL GLVDDSPAPG
60 70 80 90 100
SPGSPPPGFL LNPKFPEKVD GRFSAAPLVD LSLSPPSGLD SPNGSSSLSP
110 120 130 140 150
ECQGNGDLPP LPTAVDFQPL RYLDGVPSSF QFFLPLGSGG ALHLPASSFL
160 170 180 190 200
PPPKDKCLSP ELPLAKQLVC RWAKCNQLFE LLQDLVDHVN DHHVKPEQDA
210 220 230 240 250
RYCCHWEGCA RHGRGFNARY KMLIHIRTHT NEKPHRCPTC NKSFSRLENL
260 270 280 290 300
KIHNRSHTGE KPYVCPYEGC NKRYSNSSDR FKHTRTHYVD KPYYCKMPGC
310 320 330 340 350
HKRYTDPSSL RKHIKAHGHF VSHEQQELLQ LRPPPKPPLP TPDSGSYVSG
360 370 380 390 400
AQIIIPNPAA LFGGPSLPGL PLPLPPGPLD LSALACGNGG GGGGGIGPGL
410 420 430 440 450
PGSVLPLNLA KNPLLPSPFG AGGLGLPVVS LLGGSAGSKA EGEKGRGSVP
460 470 480 490 500
ARVLGLEDHK TPLERTERSR SRPSPDGLPL LPGTVLDLST GNSAASSPEV
510 520
LTPGWVVIPP GSVLLKPAVV N
Length:521
Mass (Da):55,842
Last modified:March 1, 2002 - v1
Checksum:i6B5F78F4A742C72D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YYX4D3YYX4_MOUSE
Zinc finger protein GLIS2
Glis2
109Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 88SG → CE in AAL93213 (PubMed:11741991).Curated2
Sequence conflicti208G → D in AAK00953 (PubMed:11741991).Curated1
Sequence conflicti210Missing in AAK28410 (PubMed:11262234).Curated1
Sequence conflicti249N → K in AAL93213 (PubMed:11741991).Curated1
Sequence conflicti315 – 318KAHG → RPW in AAL93213 (PubMed:11741991).Curated4
Sequence conflicti387G → A in AAK28410 (PubMed:11262234).Curated1
Sequence conflicti458D → N in AAL93213 (PubMed:11741991).Curated1
Sequence conflicti458D → N in AAK00953 (PubMed:11741991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249340 mRNA Translation: AAK28410.1
AF325913 mRNA Translation: AAK00953.1
AF336135 Genomic DNA Translation: AAL93213.1
BC021517 mRNA Translation: AAH21517.1
CCDSiCCDS27920.1
RefSeqiNP_112461.2, NM_031184.3
XP_006522831.1, XM_006522768.3
UniGeneiMm.134072

Genome annotation databases

EnsembliENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303
GeneIDi83396
KEGGimmu:83396
UCSCiuc007xzw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249340 mRNA Translation: AAK28410.1
AF325913 mRNA Translation: AAK00953.1
AF336135 Genomic DNA Translation: AAL93213.1
BC021517 mRNA Translation: AAH21517.1
CCDSiCCDS27920.1
RefSeqiNP_112461.2, NM_031184.3
XP_006522831.1, XM_006522768.3
UniGeneiMm.134072

3D structure databases

ProteinModelPortaliQ8VDL9
SMRiQ8VDL9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219913, 2 interactors
CORUMiQ8VDL9
STRINGi10090.ENSMUSP00000014447

PTM databases

iPTMnetiQ8VDL9
PhosphoSitePlusiQ8VDL9

Proteomic databases

PaxDbiQ8VDL9
PRIDEiQ8VDL9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303
GeneIDi83396
KEGGimmu:83396
UCSCiuc007xzw.1 mouse

Organism-specific databases

CTDi84662
MGIiMGI:1932535 Glis2

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00900000140802
HOGENOMiHOG000065778
HOVERGENiHBG101807
InParanoidiQ8VDL9
KOiK09233
OMAiDKCLSPE
OrthoDBiEOG091G0IZP
PhylomeDBiQ8VDL9
TreeFamiTF351425

Miscellaneous databases

ChiTaRSiGlis1 mouse
PROiPR:Q8VDL9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014303 Expressed in 217 organ(s), highest expression level in metanephric collecting duct
CleanExiMM_GLIS2
ExpressionAtlasiQ8VDL9 baseline and differential
GenevisibleiQ8VDL9 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 2 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGLIS2_MOUSE
AccessioniPrimary (citable) accession number: Q8VDL9
Secondary accession number(s): Q8R4X9, Q99MY6, Q99P73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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