UniProtKB - Q8VDL4 (ADPGK_MOUSE)
Protein
ADP-dependent glucokinase
Gene
Adpgk
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.
Catalytic activityi
- EC:2.7.1.147
Cofactori
Mg2+PROSITE-ProRule annotationNote: Binds 1 Mg2+ ion per subunit.PROSITE-ProRule annotation
Kineticsi
- KM=96 µM for D-glucose (at 37 degrees Celsius)1 Publication
- KM=0.28 mM for ADP (at 37 degrees Celsius)1 Publication
- KM=20 mM for ADP (at 37 degrees Celsius)1 Publication
- Vmax=160 µmol/min/mg enzyme with glucose and ADP as substrates (at 37 degrees Celsius)1 Publication
pH dependencei
Optimum pH is 5.75-6.5 and 8.75-9.0.1 Publication
: glycolysis Pathwayi
This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 297 | MagnesiumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 328 | MagnesiumPROSITE-ProRule annotation | 1 | |
Active sitei | 481 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 481 | MagnesiumPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ADP-specific glucokinase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- glucose metabolic process Source: MGI
- glycolytic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycolysis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.1.147, 3474 |
Reactomei | R-MMU-70171, Glycolysis |
SABIO-RKi | Q8VDL4 |
UniPathwayi | UPA00109 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Adpgk |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919391, Adpgk |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000184777 | 23 – 496 | ADP-dependent glucokinaseAdd BLAST | 474 |
Proteomic databases
jPOSTi | Q8VDL4 |
PaxDbi | Q8VDL4 |
PeptideAtlasi | Q8VDL4 |
PRIDEi | Q8VDL4 |
PTM databases
PhosphoSitePlusi | Q8VDL4 |
SwissPalmi | Q8VDL4 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 215179, 2 interactors |
IntActi | Q8VDL4, 1 interactor |
MINTi | Q8VDL4 |
STRINGi | 10090.ENSMUSP00000026266 |
Miscellaneous databases
RNActi | Q8VDL4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8VDL4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 52 – 496 | ADPKPROSITE-ProRule annotationAdd BLAST | 445 |
Sequence similaritiesi
Belongs to the ADP-dependent glucokinase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4184, Eukaryota |
InParanoidi | Q8VDL4 |
OrthoDBi | 1036373at2759 |
PhylomeDBi | Q8VDL4 |
TreeFami | TF313401 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR007666, ADP_PFK/GK IPR029056, Ribokinase-like |
PANTHERi | PTHR21208, PTHR21208, 1 hit |
Pfami | View protein in Pfam PF04587, ADP_PFK_GK, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS51255, ADPK, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8VDL4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP
60 70 80 90 100
LSPESRLAAA WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP
110 120 130 140 150
GSGKDHAILH SRSDLEEAFL YFMGKGAAAE RFFSDKETFH DIAQAASEFP
160 170 180 190 200
GAQHYVGGNA ALIGQRFAAN TDLKVLLCGP IGPKLHELLD DNVFVPPESL
210 220 230 240 250
QEEDEFHLIL EYLAGEEWGP FKAPHANRFI FSHDLSNGAM NMLEVFVSSL
260 270 280 290 300
EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTAISDIPT GIPVHLELAS
310 320 330 340 350
MTNRELMSSI VHQQVFPAVA SLGLNEQELL FLSQSASGPH SSLSSWDGVP
360 370 380 390 400
DVGMVSDILF WILKEHGRSE NRASDLTRIH FHTLVYHILA TVDGHWANQL
410 420 430 440 450
AAVAAGARVA GTQACATETI DTNRVSLRAP QEFTTSHLES GSRIVLNPDK
460 470 480 490
PVVEWHREGI TFHFTPVLVC KDPVRTVGLG DAISAEGLFY SEARPD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SSF2 | A0A1L1SSF2_MOUSE | ADP-dependent glucokinase | Adpgk | 496 | Annotation score: | ||
Q3UDS7 | Q3UDS7_MOUSE | ADP-dependent glucokinase | Adpgk | 495 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 318 | A → T in BAB27619 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 341 | S → A in BAB27619 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 373 | A → S in AAH21526 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013555 | 154 – 159 | HYVGGN → VCPTHQ in isoform 2. 1 Publication | 6 | |
Alternative sequenceiVSP_013556 | 160 – 496 | Missing in isoform 2. 1 PublicationAdd BLAST | 337 | |
Alternative sequenceiVSP_013557 | 313 | Missing in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK011434 mRNA Translation: BAB27619.1 AK048904 mRNA Translation: BAC33486.1 BC021526 mRNA Translation: AAH21526.1 |
CCDSi | CCDS23248.1 [Q8VDL4-1] CCDS90583.1 [Q8VDL4-3] |
RefSeqi | NP_082397.1, NM_028121.2 |
Genome annotation databases
GeneIDi | 72141 |
KEGGi | mmu:72141 |
UCSCi | uc009pxo.1, mouse [Q8VDL4-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK011434 mRNA Translation: BAB27619.1 AK048904 mRNA Translation: BAC33486.1 BC021526 mRNA Translation: AAH21526.1 |
CCDSi | CCDS23248.1 [Q8VDL4-1] CCDS90583.1 [Q8VDL4-3] |
RefSeqi | NP_082397.1, NM_028121.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5CCF | X-ray | 2.10 | A | 51-496 | [»] | |
5CK7 | X-ray | 2.99 | A | 51-496 | [»] | |
SMRi | Q8VDL4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 215179, 2 interactors |
IntActi | Q8VDL4, 1 interactor |
MINTi | Q8VDL4 |
STRINGi | 10090.ENSMUSP00000026266 |
PTM databases
PhosphoSitePlusi | Q8VDL4 |
SwissPalmi | Q8VDL4 |
Proteomic databases
jPOSTi | Q8VDL4 |
PaxDbi | Q8VDL4 |
PeptideAtlasi | Q8VDL4 |
PRIDEi | Q8VDL4 |
Genome annotation databases
GeneIDi | 72141 |
KEGGi | mmu:72141 |
UCSCi | uc009pxo.1, mouse [Q8VDL4-2] |
Organism-specific databases
CTDi | 83440 |
MGIi | MGI:1919391, Adpgk |
Phylogenomic databases
eggNOGi | KOG4184, Eukaryota |
InParanoidi | Q8VDL4 |
OrthoDBi | 1036373at2759 |
PhylomeDBi | Q8VDL4 |
TreeFami | TF313401 |
Enzyme and pathway databases
UniPathwayi | UPA00109 |
BRENDAi | 2.7.1.147, 3474 |
Reactomei | R-MMU-70171, Glycolysis |
SABIO-RKi | Q8VDL4 |
Miscellaneous databases
BioGRID-ORCSi | 72141, 0 hits in 17 CRISPR screens |
ChiTaRSi | Adpgk, mouse |
PROi | PR:Q8VDL4 |
RNActi | Q8VDL4, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR007666, ADP_PFK/GK IPR029056, Ribokinase-like |
PANTHERi | PTHR21208, PTHR21208, 1 hit |
Pfami | View protein in Pfam PF04587, ADP_PFK_GK, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS51255, ADPK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADPGK_MOUSE | |
Accessioni | Q8VDL4Primary (citable) accession number: Q8VDL4 Secondary accession number(s): Q8C7Y9, Q9D0H2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | April 26, 2005 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families