Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tetratricopeptide repeat protein 8

Gene

Ttc8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-5620922 BBSome-mediated cargo-targeting to cilium

Names & Taxonomyi

Protein namesi
Recommended name:
Tetratricopeptide repeat protein 8
Short name:
TPR repeat protein 8
Alternative name(s):
Bardet-Biedl syndrome 8 protein homolog
Gene namesi
Name:Ttc8
Synonyms:Bbs8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1923510 Ttc8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063891 – 515Tetratricopeptide repeat protein 8Add BLAST515

Proteomic databases

PaxDbiQ8VD72
PRIDEiQ8VD72

PTM databases

iPTMnetiQ8VD72
PhosphoSitePlusiQ8VD72

Expressioni

Tissue specificityi

Isoform 1 is retina-specific whereas isoform 2 is ubiquitously expressed.1 Publication

Developmental stagei

Expressed at 12 dpc in ciliated structures, including maturing (stages X and XI) spermatids, the connecting cilium of the retina and bronchial epithelial cells. At 14 and 16 dpc, detected in the telencephalon, with prominent expression at the developing ependymal cell layer and the olfactory epithelium.1 Publication

Gene expression databases

BgeeiENSMUSG00000021013 Expressed in 260 organ(s), highest expression level in retina
CleanExiMM_TTC8
ExpressionAtlasiQ8VD72 baseline and differential
GenevisibleiQ8VD72 MM

Interactioni

Subunit structurei

Part of BBSome complex, that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10. Interacts with PCM1. Interacts with CCDC28B. Interacts with PKD1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-1909 BBSome complex
DIPiDIP-60354N
IntActiQ8VD72, 4 interactors
STRINGi10090.ENSMUSP00000078148

Structurei

3D structure databases

ProteinModelPortaliQ8VD72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 37TPR 1Add BLAST34
Repeati225 – 258TPR 2Add BLAST34
Repeati259 – 291TPR 3Add BLAST33
Repeati292 – 325TPR 4Add BLAST34
Repeati326 – 359TPR 5Add BLAST34
Repeati360 – 393TPR 6Add BLAST34
Repeati397 – 430TPR 7Add BLAST34
Repeati432 – 464TPR 8Add BLAST33

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1129 Eukaryota
ENOG410Z814 LUCA
GeneTreeiENSGT00530000063455
HOGENOMiHOG000255572
HOVERGENiHBG044570
InParanoidiQ8VD72
KOiK16781
OMAiETADVWY
OrthoDBiEOG091G0FBF
PhylomeDBiQ8VD72
TreeFamiTF314892

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR028796 BBS8
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR44177 PTHR44177, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 7 hits
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8VD72-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSEMEPLLR AWSYFRRRKF QLCADLCTQM LEKSPYDQEP APDLPVSQAA
60 70 80 90 100
WILKARALTE MVYIDEIDVD QEGIAEMILD ENAIAQVPRP GTSLKLPGTN
110 120 130 140 150
QTGGPTQAVR PITQAGRPIT GFLRPSTQSG RPGTMEQAIR TPRTAYTARP
160 170 180 190 200
ITSSSGRFVR LGTASMLTSP DGPFINLSRL NLTKYSQKPK LAKALFEYIL
210 220 230 240 250
HHENDVKMAL DLASLSTEYS QYKDWWWKVQ IGKCYYRLGM YREAEKQFKS
260 270 280 290 300
ALKQQEMVDT FLYLAKVYII LDQPVTALNL FKQGLDKFPG EVTLLCGIAR
310 320 330 340 350
IYEEMNNSSS AAEYYKEVLK QDNTHVEAIA CIGSNHFYSD QPEVALRFYR
360 370 380 390 400
RLLQMGVYNC QLFNNLGLCC FYAQQYDMTL TSFERALSLA ENEEEAADVW
410 420 430 440 450
YNLGHIAVGI GDTNLAHQCF RLALVHNNHH AEAYNNLAVL EMRKGHVEQA
460 470 480 490 500
RALLQTASSL APHMYEPHFN FATVSDKIGD LQRSYVAAQK SEVAFPEHVD
510
TQHLIKQLKQ HFAML
Length:515
Mass (Da):58,440
Last modified:March 1, 2002 - v1
Checksum:i9A8ACDF59BB641C1
GO
Isoform 2 (identifier: Q8VD72-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-47: Missing.

Note: No experimental confirmation available.
Show »
Length:505
Mass (Da):57,406
Checksum:i9B13D8F8D9EB4F22
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YYH2D3YYH2_MOUSE
Tetratricopeptide repeat protein 8
Ttc8
207Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00782538 – 47Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002597 mRNA Translation: BAB22218.1
AK081697 mRNA Translation: BAC38298.1
BC017523 mRNA Translation: AAH17523.1
CCDSiCCDS26101.1 [Q8VD72-2]
CCDS26102.1 [Q8VD72-1]
RefSeqiNP_083829.1, NM_029553.3 [Q8VD72-2]
NP_938053.1, NM_198311.1 [Q8VD72-1]
UniGeneiMm.282660

Genome annotation databases

EnsembliENSMUST00000079146; ENSMUSP00000078148; ENSMUSG00000021013 [Q8VD72-1]
ENSMUST00000085109; ENSMUSP00000082190; ENSMUSG00000021013 [Q8VD72-2]
GeneIDi76260
KEGGimmu:76260
UCSCiuc007oro.2 mouse [Q8VD72-1]
uc007orp.2 mouse [Q8VD72-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002597 mRNA Translation: BAB22218.1
AK081697 mRNA Translation: BAC38298.1
BC017523 mRNA Translation: AAH17523.1
CCDSiCCDS26101.1 [Q8VD72-2]
CCDS26102.1 [Q8VD72-1]
RefSeqiNP_083829.1, NM_029553.3 [Q8VD72-2]
NP_938053.1, NM_198311.1 [Q8VD72-1]
UniGeneiMm.282660

3D structure databases

ProteinModelPortaliQ8VD72
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-1909 BBSome complex
DIPiDIP-60354N
IntActiQ8VD72, 4 interactors
STRINGi10090.ENSMUSP00000078148

PTM databases

iPTMnetiQ8VD72
PhosphoSitePlusiQ8VD72

Proteomic databases

PaxDbiQ8VD72
PRIDEiQ8VD72

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079146; ENSMUSP00000078148; ENSMUSG00000021013 [Q8VD72-1]
ENSMUST00000085109; ENSMUSP00000082190; ENSMUSG00000021013 [Q8VD72-2]
GeneIDi76260
KEGGimmu:76260
UCSCiuc007oro.2 mouse [Q8VD72-1]
uc007orp.2 mouse [Q8VD72-2]

Organism-specific databases

CTDi123016
MGIiMGI:1923510 Ttc8

Phylogenomic databases

eggNOGiKOG1129 Eukaryota
ENOG410Z814 LUCA
GeneTreeiENSGT00530000063455
HOGENOMiHOG000255572
HOVERGENiHBG044570
InParanoidiQ8VD72
KOiK16781
OMAiETADVWY
OrthoDBiEOG091G0FBF
PhylomeDBiQ8VD72
TreeFamiTF314892

Enzyme and pathway databases

ReactomeiR-MMU-5620922 BBSome-mediated cargo-targeting to cilium

Miscellaneous databases

PROiPR:Q8VD72
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021013 Expressed in 260 organ(s), highest expression level in retina
CleanExiMM_TTC8
ExpressionAtlasiQ8VD72 baseline and differential
GenevisibleiQ8VD72 MM

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR028796 BBS8
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR44177 PTHR44177, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 7 hits
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTTC8_MOUSE
AccessioniPrimary (citable) accession number: Q8VD72
Secondary accession number(s): Q9DCP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again