UniProtKB - Q8VD65 (PI3R4_MOUSE)
Phosphoinositide 3-kinase regulatory subunit 4
Pik3r4
Functioni
Catalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 53 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 148 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 32 – 40 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: GO_Central
GO - Biological processi
- autophagy of peroxisome Source: GO_Central
- cellular response to glucose starvation Source: UniProtKB
- late endosome to vacuole transport Source: GO_Central
- macroautophagy Source: GO_Central
- positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
- protein phosphorylation Source: GO_Central
- protein targeting to vacuole Source: GO_Central
- receptor catabolic process Source: MGI
- regulation of cytokinesis Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-109704, PI3K Cascade R-MMU-1632852, Macroautophagy R-MMU-1660514, Synthesis of PIPs at the Golgi membrane R-MMU-1660516, Synthesis of PIPs at the early endosome membrane R-MMU-1660517, Synthesis of PIPs at the late endosome membrane R-MMU-168138, Toll Like Receptor 9 (TLR9) Cascade R-MMU-5668599, RHO GTPases Activate NADPH Oxidases |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoinositide 3-kinase regulatory subunit 4 (EC:2.7.11.1)Short name: PI3-kinase regulatory subunit 4 |
Gene namesi | Name:Pik3r4 Synonyms:Vps151 Publication |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1922919, Pik3r4 |
Subcellular locationi
Endosome
- Late endosome By similarity
Other locations
- autophagosome Curated
- Membrane By similarity; Lipid-anchor By similarity
Note: As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (PubMed:24089209).Curated1 Publication
Cytoskeleton
- axoneme Source: UniProtKB
- microtubule cytoskeleton Source: MGI
Endosome
- late endosome Source: UniProtKB
Other locations
- autophagosome Source: UniProtKB-SubCell
- intracellular membrane-bounded organelle Source: MGI
- nucleus-vacuole junction Source: GO_Central
- phosphatidylinositol 3-kinase complex, class III Source: UniProtKB
- phosphatidylinositol 3-kinase complex, class III, type I Source: GO_Central
- phosphatidylinositol 3-kinase complex, class III, type II Source: GO_Central
Keywords - Cellular componenti
Cytoplasmic vesicle, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000086525 | 2 – 1358 | Phosphoinositide 3-kinase regulatory subunit 4Add BLAST | 1357 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 808 | PhosphoserineBy similarity | 1 | |
Modified residuei | 813 | PhosphoserineBy similarity | 1 | |
Modified residuei | 853 | PhosphoserineBy similarity | 1 | |
Modified residuei | 865 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1316 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
EPDi | Q8VD65 |
MaxQBi | Q8VD65 |
PaxDbi | Q8VD65 |
PRIDEi | Q8VD65 |
PTM databases
iPTMneti | Q8VD65 |
PhosphoSitePlusi | Q8VD65 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032571, Expressed in primary oocyte and 285 other tissues |
ExpressionAtlasi | Q8VD65, baseline and differential |
Genevisiblei | Q8VD65, MM |
Interactioni
Subunit structurei
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG (PubMed:23332761). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2 (By similarity). PI3KC3-C1 probably associates with PIK3CB (PubMed:21059846).
Interacts with RAB7A in the presence of PIK3C3/VPS34 (By similarity).
Interacts with NRBF2 (By similarity).
By similarity2 PublicationsBinary interactionsi
Q8VD65
With | #Exp. | IntAct |
---|---|---|
Gja1 [P23242] | 3 | EBI-6678184,EBI-298630 |
Protein-protein interaction databases
BioGRIDi | 217660, 14 interactors |
ComplexPortali | CPX-75, Phosphatidylinositol 3-kinase complex, class III, ATG14 variant CPX-76, Phosphatidylinositol 3-kinase complex, class III, UVRAG variant |
DIPi | DIP-57219N |
IntActi | Q8VD65, 7 interactors |
MINTi | Q8VD65 |
STRINGi | 10090.ENSMUSP00000067400 |
Miscellaneous databases
RNActi | Q8VD65, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 324 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 299 | |
Repeati | 413 – 450 | HEAT 1Add BLAST | 38 | |
Repeati | 458 – 495 | HEAT 2Add BLAST | 38 | |
Repeati | 572 – 610 | HEAT 3Add BLAST | 39 | |
Repeati | 612 – 648 | HEAT 4Add BLAST | 37 | |
Repeati | 991 – 1030 | WD 1Add BLAST | 40 | |
Repeati | 1040 – 1079 | WD 2Add BLAST | 40 | |
Repeati | 1093 – 1134 | WD 3Add BLAST | 42 | |
Repeati | 1139 – 1178 | WD 4Add BLAST | 40 | |
Repeati | 1182 – 1223 | WD 5Add BLAST | 42 | |
Repeati | 1237 – 1278 | WD 6Add BLAST | 42 | |
Repeati | 1327 – 1358 | WD 7Add BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 781 – 784 | Poly-Glu | 4 | |
Compositional biasi | 974 – 977 | Poly-Pro | 4 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG1240, Eukaryota |
GeneTreei | ENSGT00390000016225 |
HOGENOMi | CLU_001696_0_0_1 |
InParanoidi | Q8VD65 |
OMAi | LNDRDWM |
OrthoDBi | 1312453at2759 |
PhylomeDBi | Q8VD65 |
TreeFami | TF102034 |
Family and domain databases
Gene3Di | 1.25.10.10, 2 hits 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR021133, HEAT_type_2 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00400, WD40, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit SM00320, WD40, 6 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF50978, SSF50978, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGNQLAGIAP SQILSVESYF SDIHDFEYDK SLGSTRFFKV ARAKHREGLV
60 70 80 90 100
VVKVFAIQDP TLPLTSYKQE LEELKIRLHS AQNCLPFQKA AEKASEKAAM
110 120 130 140 150
LFRQYVRDNL YDRISTRPFL NNIEKRWIAF QILTAVDQAH KSGVRHGDIK
160 170 180 190 200
TENVMVTSWN WVLLTDFASF KPTYLPEDNP ADFNYFFDTS RRRTCYIAPE
210 220 230 240 250
RFVDGGMFAT ELEYMRDPST PLVDLNSNQR ARGELKRAMD IFSAGCVIAE
260 270 280 290 300
LFTEGVPLFD LSQLLAYRNG HFFPEQVLNK IEDRSIRDLV TQMINREPEK
310 320 330 340 350
RLEAEDYLKQ QRGNAFPEIF YTFLQPYMAQ FAKETFLSAD ERILVIRKDL
360 370 380 390 400
GNIIHNLCGH DLPEKAEGES RASGLVVLVS VITSCLQTLK SCDSKLAALE
410 420 430 440 450
LILHLAPRLS VEILLDRITP YLLHFSNDSV PRVRAEALRT LTKVLALVQE
460 470 480 490 500
VPRNDVNIYP EYILPGIAHL AQDDATIVRL AYAENIALLA ETALRFLELV
510 520 530 540 550
QLKTLNMENE PDNEEVDEAT RPNGDYDTEL QALHEMVQQK VVTLLSDPEN
560 570 580 590 600
IVKQTLMESG ITRLCVFFGR QKANDVLLSH MITFLNDKND WHLRGAFFDS
610 620 630 640 650
IVGVAAYVGW QSSSILKPLL QQGLSDAEEF VIVKALNALT CMCQLGLLQK
660 670 680 690 700
PHVYEFASDI APFLCHPNLW IRYGAVGFIT VVAHQISTAD VYCKLMPYLD
710 720 730 740 750
PYITQPVIQI ERKLVLLSVL KEPVSRSIFD YALRSKDIAS LFRHLHMRQK
760 770 780 790 800
KRNGSLLDCP PPEDPTIAQL LKKLLSQGMT EEEEDKLLAL KDFMMKSNRA
810 820 830 840 850
KANAVDQSHL HDSSQKGVID LAALGITGRQ VDLVKTKQEP DEKRARKHVK
860 870 880 890 900
QDSNVNEEWK SMFGSLEPPN IPQALPKTSD HEVVQPGKPP RSESSAGICV
910 920 930 940 950
PLSTSPQVSE AAHIPSKKPV IPVVSSTVLP STYQIRITTC KTELQQLIQQ
960 970 980 990 1000
KREQCNAERI AKQMMENAEW ESKPPPPGWR PKGLLVAHLH EHKSAVNRIR
1010 1020 1030 1040 1050
VSDEHLLFAT CSNDGTVKIW NSQKMEGKTT TTRSILTYSR IGGRVKTLTF
1060 1070 1080 1090 1100
CQGSHYLAIA SDNGAVQLLG IEASKLPKSP KIHPLQSRIL DQKEDGCVVD
1110 1120 1130 1140 1150
MHHFNSGAQS VLAYATVNGS LVGWDLRSSS NAWTLKHDLK SGLITSFAVD
1160 1170 1180 1190 1200
IHQCWLCIGT SSGAMACWDM RFQLPISSHC HPSRARIRRL SMHPLYQSWV
1210 1220 1230 1240 1250
IAAVQGNNEV SMWDMETGDR RLTLWASSAP PLSELQPSPH SVHGIYCSPA
1260 1270 1280 1290 1300
DGNPILLTAG SDMKIRFWDL VSPERSYVVA GSTGSPSVSY YKKIIEGTEV
1310 1320 1330 1340 1350
VQEIQNKQKV GPSDDTPRRG PESLPVGHHD IITDIATFQT TQGFIVTASR
DGIVKVWK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WP62 | A0A087WP62_MOUSE | Phosphoinositide 3-kinase regulator... | Pik3r4 | 174 | Annotation score: | ||
A0A0R4J2C7 | A0A0R4J2C7_MOUSE | Phosphoinositide 3-kinase regulator... | Pik3r4 | 305 | Annotation score: | ||
A0A087WQR4 | A0A087WQR4_MOUSE | Phosphoinositide 3-kinase regulator... | Pik3r4 | 208 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 292 – 305 | QMINR…RLEAE → MLQIRAIEFYLTMS in BAC31236 (PubMed:16141072).CuratedAdd BLAST | 14 | |
Sequence conflicti | 511 | P → S in BAC31417 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 529 – 603 | ELQAL…DSIVG → GCVTPLLKSPSFTPNRYWYL VKAVICDSLGK in BAC31417 (PubMed:16141072).CuratedAdd BLAST | 75 | |
Sequence conflicti | 529 – 559 | ELQAL…TLMES → GCVTPLLKSPSFTPNRYWYL VKAVICDSLG in BAC31417 (PubMed:16141072).CuratedAdd BLAST | 31 | |
Sequence conflicti | 621 – 637 | QQGLS…VKALN → HAPPVPVLGNRSCSGQQ in AAH92149 (PubMed:15489334).CuratedAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC157514 Genomic DNA No translation available. AK008703 mRNA Translation: BAB25843.1 AK042361 mRNA Translation: BAC31236.1 AK042953 mRNA Translation: BAC31417.1 AK087938 mRNA No translation available. BC017537 mRNA Translation: AAH17537.1 BC092149 mRNA Translation: AAH92149.1 |
CCDSi | CCDS40753.1 |
RefSeqi | NP_001074778.1, NM_001081309.1 |
Genome annotation databases
Ensembli | ENSMUST00000065778; ENSMUSP00000067400; ENSMUSG00000032571 ENSMUST00000191268; ENSMUSP00000139427; ENSMUSG00000032571 |
GeneIDi | 75669 |
KEGGi | mmu:75669 |
UCSCi | uc009rio.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC157514 Genomic DNA No translation available. AK008703 mRNA Translation: BAB25843.1 AK042361 mRNA Translation: BAC31236.1 AK042953 mRNA Translation: BAC31417.1 AK087938 mRNA No translation available. BC017537 mRNA Translation: AAH17537.1 BC092149 mRNA Translation: AAH92149.1 |
CCDSi | CCDS40753.1 |
RefSeqi | NP_001074778.1, NM_001081309.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 217660, 14 interactors |
ComplexPortali | CPX-75, Phosphatidylinositol 3-kinase complex, class III, ATG14 variant CPX-76, Phosphatidylinositol 3-kinase complex, class III, UVRAG variant |
DIPi | DIP-57219N |
IntActi | Q8VD65, 7 interactors |
MINTi | Q8VD65 |
STRINGi | 10090.ENSMUSP00000067400 |
PTM databases
iPTMneti | Q8VD65 |
PhosphoSitePlusi | Q8VD65 |
Proteomic databases
EPDi | Q8VD65 |
MaxQBi | Q8VD65 |
PaxDbi | Q8VD65 |
PRIDEi | Q8VD65 |
Protocols and materials databases
Antibodypediai | 33331, 450 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000065778; ENSMUSP00000067400; ENSMUSG00000032571 ENSMUST00000191268; ENSMUSP00000139427; ENSMUSG00000032571 |
GeneIDi | 75669 |
KEGGi | mmu:75669 |
UCSCi | uc009rio.1, mouse |
Organism-specific databases
CTDi | 30849 |
MGIi | MGI:1922919, Pik3r4 |
Phylogenomic databases
eggNOGi | KOG1240, Eukaryota |
GeneTreei | ENSGT00390000016225 |
HOGENOMi | CLU_001696_0_0_1 |
InParanoidi | Q8VD65 |
OMAi | LNDRDWM |
OrthoDBi | 1312453at2759 |
PhylomeDBi | Q8VD65 |
TreeFami | TF102034 |
Enzyme and pathway databases
Reactomei | R-MMU-109704, PI3K Cascade R-MMU-1632852, Macroautophagy R-MMU-1660514, Synthesis of PIPs at the Golgi membrane R-MMU-1660516, Synthesis of PIPs at the early endosome membrane R-MMU-1660517, Synthesis of PIPs at the late endosome membrane R-MMU-168138, Toll Like Receptor 9 (TLR9) Cascade R-MMU-5668599, RHO GTPases Activate NADPH Oxidases |
Miscellaneous databases
BioGRID-ORCSi | 75669, 6 hits in 17 CRISPR screens |
ChiTaRSi | Pik3r4, mouse |
PROi | PR:Q8VD65 |
RNActi | Q8VD65, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032571, Expressed in primary oocyte and 285 other tissues |
ExpressionAtlasi | Q8VD65, baseline and differential |
Genevisiblei | Q8VD65, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 2 hits 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR021133, HEAT_type_2 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00400, WD40, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit SM00320, WD40, 6 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF50978, SSF50978, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PI3R4_MOUSE | |
Accessioni | Q8VD65Primary (citable) accession number: Q8VD65 Secondary accession number(s): Q56A65 Q9CVC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index