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Protein

Glycosaminoglycan xylosylkinase

Gene

Fam20b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized.By similarity

Catalytic activityi

3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-seryl-[protein] + ATP = 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-2-O-phosphoxylosyl)-L-seryl-[protein] + ADP.By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATP; via amide nitrogenBy similarity1
Binding sitei123ATPBy similarity1
Metal bindingi142ManganeseBy similarity1
Active sitei289By similarity1
Binding sitei294ATPBy similarity1
Metal bindingi309ManganeseBy similarity1
Binding sitei309ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 225ATPBy similarity4

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glycosaminoglycan xylosylkinaseBy similarity (EC:2.7.1.-By similarity)
Alternative name(s):
Xylose kinase
Gene namesi
Name:Fam20bImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443990 Fam20b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini26 – 409LumenalSequence analysisAdd BLAST384

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryos show severe stunting and increased mortality at E13.5.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000087461 – 409Glycosaminoglycan xylosylkinaseAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi193N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi196 ↔ 211By similarity
Disulfide bondi201 ↔ 204By similarity
Disulfide bondi257 ↔ 331By similarity
Disulfide bondi332 ↔ 389By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8VCS3
MaxQBiQ8VCS3
PaxDbiQ8VCS3
PeptideAtlasiQ8VCS3
PRIDEiQ8VCS3

PTM databases

PhosphoSitePlusiQ8VCS3
SwissPalmiQ8VCS3

Expressioni

Tissue specificityi

Little expression detected in the secretory stage enamel. Weak to moderate expression is observed in the gingivae and odontoblasts. Strong expression in maturation stage ameloblasts.1 Publication

Gene expression databases

BgeeiENSMUSG00000033557 Expressed in 273 organ(s), highest expression level in cornea
GenevisibleiQ8VCS3 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083322

Structurei

3D structure databases

ProteinModelPortaliQ8VCS3
SMRiQ8VCS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FAM20 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3829 Eukaryota
ENOG410XQEJ LUCA
GeneTreeiENSGT00390000007484
HOGENOMiHOG000231437
HOVERGENiHBG051635
InParanoidiQ8VCS3
KOiK20825
OMAiAFHRMMA
OrthoDBiEOG091G1BEO
PhylomeDBiQ8VCS3
TreeFamiTF313276

Family and domain databases

InterProiView protein in InterPro
IPR024869 FAM20
IPR009581 FAM20_C
PANTHERiPTHR12450 PTHR12450, 1 hit
PfamiView protein in Pfam
PF06702 Fam20C, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VCS3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLKQRVVLL AILLVIFIFT KVFLIDNLDT SAANREDQRA FHRMMTGLRV
60 70 80 90 100
ELVPKLDHTL QSPWEIAAQW VVPREVYPEE TPELGAIMHA MATKKIIKAD
110 120 130 140 150
VGYKGTQLKA LLILEGGQKV VFKPKRYSRD YVVEGEPYAG YDRHNAEVAA
160 170 180 190 200
FHLDRILGFR RAPLVVGRYV NLRTEVKPVA TEQLLSTFLT VGNNTCFYGK
210 220 230 240 250
CYYCRETEPA CADGDMMEGS VTLWLPDVWP LQKHRHPWGR TYREGKLARW
260 270 280 290 300
EYDESYCDAV KKTSPYDSGP RLLDIIDTAV FDYLIGNADR HHYESFQDDE
310 320 330 340 350
GASMLILLDN AKSFGNPSLD ERSILAPLYQ CCIIRVSTWN RLNYLKNGVL
360 370 380 390 400
KSALKSAMAH DPISPVLSDP HLDTVDQRLL NVLATIKQCT DQFGTDTVLV

EDRMPLSHL
Length:409
Mass (Da):46,581
Last modified:March 1, 2002 - v1
Checksum:i4BDD0C89D48C4187
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049705 mRNA Translation: BAC33883.1
AK132149 mRNA Translation: BAE20998.1
CH466520 Genomic DNA Translation: EDL39377.1
BC019381 mRNA Translation: AAH19381.1
BC023737 mRNA Translation: AAH23737.1
BC024412 mRNA Translation: AAH24412.1
BC031473 mRNA Translation: AAH31473.1
AK129154 mRNA Translation: BAC97964.1
CCDSiCCDS15395.1
RefSeqiNP_663388.1, NM_145413.4
XP_006496799.1, XM_006496736.2
XP_006496800.1, XM_006496737.2
UniGeneiMm.229107

Genome annotation databases

EnsembliENSMUST00000086153; ENSMUSP00000083322; ENSMUSG00000033557
ENSMUST00000122424; ENSMUSP00000112534; ENSMUSG00000033557
GeneIDi215015
KEGGimmu:215015
UCSCiuc007dcp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049705 mRNA Translation: BAC33883.1
AK132149 mRNA Translation: BAE20998.1
CH466520 Genomic DNA Translation: EDL39377.1
BC019381 mRNA Translation: AAH19381.1
BC023737 mRNA Translation: AAH23737.1
BC024412 mRNA Translation: AAH24412.1
BC031473 mRNA Translation: AAH31473.1
AK129154 mRNA Translation: BAC97964.1
CCDSiCCDS15395.1
RefSeqiNP_663388.1, NM_145413.4
XP_006496799.1, XM_006496736.2
XP_006496800.1, XM_006496737.2
UniGeneiMm.229107

3D structure databases

ProteinModelPortaliQ8VCS3
SMRiQ8VCS3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083322

PTM databases

PhosphoSitePlusiQ8VCS3
SwissPalmiQ8VCS3

Proteomic databases

EPDiQ8VCS3
MaxQBiQ8VCS3
PaxDbiQ8VCS3
PeptideAtlasiQ8VCS3
PRIDEiQ8VCS3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086153; ENSMUSP00000083322; ENSMUSG00000033557
ENSMUST00000122424; ENSMUSP00000112534; ENSMUSG00000033557
GeneIDi215015
KEGGimmu:215015
UCSCiuc007dcp.1 mouse

Organism-specific databases

CTDi9917
MGIiMGI:2443990 Fam20b

Phylogenomic databases

eggNOGiKOG3829 Eukaryota
ENOG410XQEJ LUCA
GeneTreeiENSGT00390000007484
HOGENOMiHOG000231437
HOVERGENiHBG051635
InParanoidiQ8VCS3
KOiK20825
OMAiAFHRMMA
OrthoDBiEOG091G1BEO
PhylomeDBiQ8VCS3
TreeFamiTF313276

Miscellaneous databases

ChiTaRSiFam20b mouse
PROiPR:Q8VCS3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033557 Expressed in 273 organ(s), highest expression level in cornea
GenevisibleiQ8VCS3 MM

Family and domain databases

InterProiView protein in InterPro
IPR024869 FAM20
IPR009581 FAM20_C
PANTHERiPTHR12450 PTHR12450, 1 hit
PfamiView protein in Pfam
PF06702 Fam20C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiXYLK_MOUSE
AccessioniPrimary (citable) accession number: Q8VCS3
Secondary accession number(s): Q3V201, Q6ZQA3, Q8BWX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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