UniProtKB - Q8VCQ6 (SMS1_MOUSE)
Phosphatidylcholine:ceramide cholinephosphotransferase 1
Sgms1
Functioni
Major sphingomyelin synthase at the Golgi apparatus (By similarity).
Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes (By similarity).
Does not use free phosphorylcholine or CDP-choline as donor. Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting (PubMed:22580896, PubMed:16879426) (By similarity).
Plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1 (By similarity).
By similarity2 Publications(Microbial infection) Contributes to the brain SM production for Japanese encephalitis virus attachment and infection.
1 PublicationCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = a 1,2-diacyl-sn-glycerol + a sphingomyelin1 PublicationEC:2.7.8.271 Publication
: Sphingolipid metabolism Pathwayi
This protein is involved in Sphingolipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in Sphingolipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 291 | By similarity | 1 | |
Active sitei | 334 | By similarity | 1 | |
Active sitei | 338 | By similarity | 1 |
GO - Molecular functioni
- ceramide cholinephosphotransferase activity Source: UniProtKB
- ceramide phosphoethanolamine synthase activity Source: MGI
- kinase activity Source: UniProtKB-KW
- sphingomyelin synthase activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cellular response to lipopolysaccharide Source: Ensembl
- cellular response to tumor necrosis factor Source: Ensembl
- ceramide biosynthetic process Source: MGI
- inflammatory response Source: MGI
- positive regulation of gene expression Source: MGI
- sphingomyelin biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Apoptosis, Lipid metabolism, Sphingolipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.7.8.27, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylcholine:ceramide cholinephosphotransferase 1 (EC:2.7.8.271 Publication)Alternative name(s): Protein Mob Sphingomyelin synthase 1 Transmembrane protein 23 |
Gene namesi | Name:Sgms1 Synonyms:Tmem23 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2444110, Sgms1 |
VEuPathDBi | HostDB:ENSMUSG00000040451 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: HGNC-UCL
- integral component of endoplasmic reticulum membrane Source: GO_Central
Golgi apparatus
- Golgi trans cisterna Source: MGI
- integral component of Golgi membrane Source: UniProtKB
Nucleus
- nucleus Source: HGNC-UCL
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: HGNC-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 190 – 210 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 221 – 241 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 282 – 302 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 310 – 330 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 331 – 419 | CytoplasmicSequence analysisAdd BLAST | 89 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL4523408 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000221069 | 1 – 419 | Phosphatidylcholine:ceramide cholinephosphotransferase 1Add BLAST | 419 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q8VCQ6 |
PaxDbi | Q8VCQ6 |
PRIDEi | Q8VCQ6 |
ProteomicsDBi | 261459 [Q8VCQ6-1] 261460 [Q8VCQ6-2] 261461 [Q8VCQ6-3] |
PTM databases
PhosphoSitePlusi | Q8VCQ6 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000040451, Expressed in brain blood vessel and 308 other tissues |
ExpressionAtlasi | Q8VCQ6, baseline and differential |
Genevisiblei | Q8VCQ6, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000117336 |
Chemistry databases
BindingDBi | Q8VCQ6 |
Miscellaneous databases
RNActi | Q8VCQ6, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8VCQ6 |
SMRi | Q8VCQ6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8VCQ6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 76 | SAMPROSITE-ProRule annotationCuratedAdd BLAST | 64 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3058, Eukaryota |
GeneTreei | ENSGT00940000158306 |
HOGENOMi | CLU_027104_1_0_1 |
InParanoidi | Q8VCQ6 |
OMAi | WICWTLS |
OrthoDBi | 599210at2759 |
PhylomeDBi | Q8VCQ6 |
TreeFami | TF314547 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR001660, SAM IPR013761, SAM/pointed_sf IPR045221, Sphingomyelin_synth-like IPR025749, Sphingomyelin_synth-like_dom |
PANTHERi | PTHR21290, PTHR21290, 1 hit |
Pfami | View protein in Pfam PF14360, PAP2_C, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50105, SAM_DOMAIN, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLSARTMKEV VYWSPKKVAD WLLENAMPEY CEPLEHFTGQ DLINLTQEDF
60 70 80 90 100
KKPPLYRVSS DNGQRLLDMI ETLKMEHHME AHKNGHANGH LSIGVDIPNP
110 120 130 140 150
DGSFSIKTKP NGMPNGFRKE MIKIPMPEPE RSQYPMEWGK TFLAFLYALS
160 170 180 190 200
CFVLTTVMIS VVHERVPPKE VQPPLPDTFF DHFNRVQWAF SICEINGMIL
210 220 230 240 250
VGLWLFQWLL LKYKSIISRR FFCIVGTLYL YRCITMYVTT LPVPGMHFNC
260 270 280 290 300
SPKLFGDWEA QVRRIMKLIA GGGLSITGSH NMCGDYLYSG HTVMLTLTYL
310 320 330 340 350
FIKEYSPRRL WWYHWICWLL SVVGIFCILL AHDHYTVDVV VAYYITTRLF
360 370 380 390 400
WWYHTMANQQ VLKEASQMNL LARVWWYRPF QYFEKNVQGI VPRSYHWPFP
410
WPVVHLSRQV KYSRLVNDT
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE3W996 | E3W996_MOUSE | Phosphatidylcholine:ceramide cholin... | Sgms1 | 220 | Annotation score: | ||
A0A494BA77 | A0A494BA77_MOUSE | Phosphatidylcholine:ceramide cholin... | Sgms1 | 293 | Annotation score: | ||
D3YV54 | D3YV54_MOUSE | Phosphatidylcholine:ceramide cholin... | Sgms1 | 34 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | S → Y in BAC37590 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 406 | L → I in BAE27428 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050673 | 215 – 419 | Missing in isoform 2. 2 PublicationsAdd BLAST | 205 | |
Alternative sequenceiVSP_027225 | 215 – 217 | SII → LSP in isoform 3. 2 Publications | 3 | |
Alternative sequenceiVSP_027226 | 218 – 419 | Missing in isoform 3. 2 PublicationsAdd BLAST | 202 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY509044 mRNA Translation: AAS90514.1 AK076554 mRNA Translation: BAC36390.1 AK079254 mRNA Translation: BAC37590.1 AK082974 mRNA Translation: BAC38717.1 AK133967 mRNA Translation: BAE21960.1 AK146782 mRNA Translation: BAE27428.1 AK150272 mRNA Translation: BAE29428.1 AK151170 mRNA Translation: BAE30173.1 AK152312 mRNA Translation: BAE31117.1 AK153406 mRNA Translation: BAE31966.1 AK160539 mRNA Translation: BAE35856.1 BC019443 mRNA Translation: AAH19443.2 BC085298 mRNA Translation: AAH85298.1 |
CCDSi | CCDS29750.1 [Q8VCQ6-1] |
RefSeqi | NP_001161997.1, NM_001168525.1 [Q8VCQ6-1] NP_001161998.1, NM_001168526.1 [Q8VCQ6-1] NP_659041.3, NM_144792.4 [Q8VCQ6-1] XP_006526881.1, XM_006526818.3 [Q8VCQ6-1] XP_006526882.1, XM_006526819.1 [Q8VCQ6-1] XP_006526883.1, XM_006526820.3 [Q8VCQ6-1] XP_011245503.1, XM_011247201.2 XP_011245504.1, XM_011247202.2 [Q8VCQ6-1] XP_011245505.1, XM_011247203.2 [Q8VCQ6-1] XP_017173594.1, XM_017318105.1 [Q8VCQ6-3] |
Genome annotation databases
Ensembli | ENSMUST00000099514; ENSMUSP00000097114; ENSMUSG00000040451 [Q8VCQ6-1] ENSMUST00000142618; ENSMUSP00000117336; ENSMUSG00000040451 [Q8VCQ6-1] ENSMUST00000151289; ENSMUSP00000123395; ENSMUSG00000040451 [Q8VCQ6-1] ENSMUST00000152340; ENSMUSP00000119869; ENSMUSG00000040451 [Q8VCQ6-3] |
GeneIDi | 208449 |
KEGGi | mmu:208449 |
UCSCi | uc008hfc.2, mouse [Q8VCQ6-1] uc008hfh.1, mouse [Q8VCQ6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY509044 mRNA Translation: AAS90514.1 AK076554 mRNA Translation: BAC36390.1 AK079254 mRNA Translation: BAC37590.1 AK082974 mRNA Translation: BAC38717.1 AK133967 mRNA Translation: BAE21960.1 AK146782 mRNA Translation: BAE27428.1 AK150272 mRNA Translation: BAE29428.1 AK151170 mRNA Translation: BAE30173.1 AK152312 mRNA Translation: BAE31117.1 AK153406 mRNA Translation: BAE31966.1 AK160539 mRNA Translation: BAE35856.1 BC019443 mRNA Translation: AAH19443.2 BC085298 mRNA Translation: AAH85298.1 |
CCDSi | CCDS29750.1 [Q8VCQ6-1] |
RefSeqi | NP_001161997.1, NM_001168525.1 [Q8VCQ6-1] NP_001161998.1, NM_001168526.1 [Q8VCQ6-1] NP_659041.3, NM_144792.4 [Q8VCQ6-1] XP_006526881.1, XM_006526818.3 [Q8VCQ6-1] XP_006526882.1, XM_006526819.1 [Q8VCQ6-1] XP_006526883.1, XM_006526820.3 [Q8VCQ6-1] XP_011245503.1, XM_011247201.2 XP_011245504.1, XM_011247202.2 [Q8VCQ6-1] XP_011245505.1, XM_011247203.2 [Q8VCQ6-1] XP_017173594.1, XM_017318105.1 [Q8VCQ6-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2D8C | NMR | - | A | 1-84 | [»] | |
AlphaFoldDBi | Q8VCQ6 | |||||
SMRi | Q8VCQ6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000117336 |
Chemistry databases
BindingDBi | Q8VCQ6 |
ChEMBLi | CHEMBL4523408 |
PTM databases
PhosphoSitePlusi | Q8VCQ6 |
Proteomic databases
MaxQBi | Q8VCQ6 |
PaxDbi | Q8VCQ6 |
PRIDEi | Q8VCQ6 |
ProteomicsDBi | 261459 [Q8VCQ6-1] 261460 [Q8VCQ6-2] 261461 [Q8VCQ6-3] |
Protocols and materials databases
Antibodypediai | 27870, 201 antibodies from 25 providers |
DNASUi | 208449 |
Genome annotation databases
Ensembli | ENSMUST00000099514; ENSMUSP00000097114; ENSMUSG00000040451 [Q8VCQ6-1] ENSMUST00000142618; ENSMUSP00000117336; ENSMUSG00000040451 [Q8VCQ6-1] ENSMUST00000151289; ENSMUSP00000123395; ENSMUSG00000040451 [Q8VCQ6-1] ENSMUST00000152340; ENSMUSP00000119869; ENSMUSG00000040451 [Q8VCQ6-3] |
GeneIDi | 208449 |
KEGGi | mmu:208449 |
UCSCi | uc008hfc.2, mouse [Q8VCQ6-1] uc008hfh.1, mouse [Q8VCQ6-2] |
Organism-specific databases
CTDi | 259230 |
MGIi | MGI:2444110, Sgms1 |
VEuPathDBi | HostDB:ENSMUSG00000040451 |
Phylogenomic databases
eggNOGi | KOG3058, Eukaryota |
GeneTreei | ENSGT00940000158306 |
HOGENOMi | CLU_027104_1_0_1 |
InParanoidi | Q8VCQ6 |
OMAi | WICWTLS |
OrthoDBi | 599210at2759 |
PhylomeDBi | Q8VCQ6 |
TreeFami | TF314547 |
Enzyme and pathway databases
BRENDAi | 2.7.8.27, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 208449, 10 hits in 75 CRISPR screens |
ChiTaRSi | Sgms1, mouse |
EvolutionaryTracei | Q8VCQ6 |
PROi | PR:Q8VCQ6 |
RNActi | Q8VCQ6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040451, Expressed in brain blood vessel and 308 other tissues |
ExpressionAtlasi | Q8VCQ6, baseline and differential |
Genevisiblei | Q8VCQ6, MM |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR001660, SAM IPR013761, SAM/pointed_sf IPR045221, Sphingomyelin_synth-like IPR025749, Sphingomyelin_synth-like_dom |
PANTHERi | PTHR21290, PTHR21290, 1 hit |
Pfami | View protein in Pfam PF14360, PAP2_C, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50105, SAM_DOMAIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMS1_MOUSE | |
Accessioni | Q8VCQ6Primary (citable) accession number: Q8VCQ6 Secondary accession number(s): Q3UIS8 Q8C652 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | February 16, 2004 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families