UniProtKB - Q8VCQ3 (NRBF2_MOUSE)
Nuclear receptor-binding factor 2
Nrbf2
Functioni
May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro) (By similarity).
By similarityInvolved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy (By similarity).
Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (PubMed:24849286).
Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1 (By similarity).
May be involved in autophagosome biogenesis (By similarity).
May play a role in neural progenitor cell survival during differentiation (PubMed:18619852).
By similarity2 PublicationsInvolved in the induction of starvation-induced autophagy. Modulates ATG14-linked lipid kinase activity of PIK3C3 and stabilzes PI3K complex I (PI3KC3-C1) assembly (PubMed:24849286).
May play a role in neural progenitor cell survival during differentiation (PubMed:18619852).
By similarity2 PublicationsGO - Molecular functioni
- nuclear receptor binding Source: MGI
- transcription coactivator activity Source: MGI
GO - Biological processi
- autophagy Source: GO_Central
- positive regulation of transcription, DNA-templated Source: MGI
- regulation of lipid kinase activity Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
- response to endoplasmic reticulum stress Source: UniProtKB
Keywordsi
Biological process | Autophagy, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor-binding factor 2Short name: NRBF-2 |
Gene namesi | Name:Nrbf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354950, Nrbf2 |
VEuPathDBi | HostDB:ENSMUSG00000075000 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- Nucleus By similarity
Other locations
- autophagosome Curated
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- autophagosome Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: UniProtKB-KW
- phosphatidylinositol 3-kinase complex, class III Source: Ensembl
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235817 | 1 – 287 | Nuclear receptor-binding factor 2Add BLAST | 287 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 112 | PhosphoserineBy similarity | 1 | |
Modified residuei | 268 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8VCQ3 |
MaxQBi | Q8VCQ3 |
PaxDbi | Q8VCQ3 |
PeptideAtlasi | Q8VCQ3 |
PRIDEi | Q8VCQ3 |
ProteomicsDBi | 293970 |
PTM databases
iPTMneti | Q8VCQ3 |
PhosphoSitePlusi | Q8VCQ3 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000075000, Expressed in liver and 74 other tissues |
Genevisiblei | Q8VCQ3, MM |
Interactioni
Subunit structurei
Interacts with RRARA, PPARD and PPARG.
Interacts with THRB, RARA, RARG and RXRA in the presence of bound ligand (By similarity).
Interacts with SCOC (By similarity). Associates with the PI3K complex I (PI3KC3-C1); the direct binding partner in the complex is reported variably as PIK3R4 or ATG14 (PubMed:24849286).
By similarity1 PublicationBinary interactionsi
GO - Molecular functioni
- nuclear receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 565935, 1 interactor |
IntActi | Q8VCQ3, 8 interactors |
STRINGi | 10090.ENSMUSP00000097254 |
Miscellaneous databases
RNActi | Q8VCQ3, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8VCQ3 |
SMRi | Q8VCQ3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8VCQ3 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 81 – 122 | DisorderedSequence analysisAdd BLAST | 42 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 168 – 215 | Sequence analysisAdd BLAST | 48 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 141 – 145 | Nuclear receptor interaction motifBy similarity | 5 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502SGH4, Eukaryota |
GeneTreei | ENSGT00390000000984 |
HOGENOMi | CLU_098323_0_0_1 |
InParanoidi | Q8VCQ3 |
OMAi | CEAMKLT |
OrthoDBi | 1613014at2759 |
PhylomeDBi | Q8VCQ3 |
TreeFami | TF328627 |
Family and domain databases
IDEALi | IID50293 |
InterProi | View protein in InterPro IPR039679, NRBF2 IPR015056, NRBF2_C IPR033393, NRBF2_MIT |
PANTHERi | PTHR14964, PTHR14964, 1 hit |
Pfami | View protein in Pfam PF08961, NRBF2, 1 hit PF17169, NRBF2_MIT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEVMEGPLNL AHQQSRRADR LLAAGKYEEA ISCHRKATTY LSEAMKLTES
60 70 80 90 100
EQAHLSLELQ RDSHMKQLLL IQERWKRAKR EERLKAQQST ERDGAPHLQA
110 120 130 140 150
PPRPSEDAEG QSPLLSQPYI PSTERRLPEV QGVFDRDPDT LLFLLQQKNE
160 170 180 190 200
PSEPCIGSKA PKDDKTIIEE QATKIADLKR HVEFLVAENE RLRKENKQLK
210 220 230 240 250
AEKARLLKGT AEKELDVDAD FVEKSELWGL PSHSESAAAS STWQKFAANT
260 270 280
GKAKDIPIPN LPPLDFPSPE LPLMELSEDI LKGFMND
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CZ57 | E0CZ57_MOUSE | Nuclear receptor-binding factor 2 | Nrbf2 | 60 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 203 | K → E in BAB22375 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 245 – 264 | KFAAN…NLPPL → SLQLTRGSQGHSNTQPSSS in BAB22375 (PubMed:16141072).CuratedAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002810 mRNA Translation: BAB22375.1 BC019448 mRNA Translation: AAH19448.1 |
CCDSi | CCDS23902.1 |
RefSeqi | NP_001031370.1, NM_001036293.2 |
Genome annotation databases
Ensembli | ENSMUST00000077839; ENSMUSP00000097254; ENSMUSG00000075000 |
GeneIDi | 641340 |
KEGGi | mmu:641340 |
UCSCi | uc007flw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002810 mRNA Translation: BAB22375.1 BC019448 mRNA Translation: AAH19448.1 |
CCDSi | CCDS23902.1 |
RefSeqi | NP_001031370.1, NM_001036293.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CRB | NMR | - | A | 4-87 | [»] | |
AlphaFoldDBi | Q8VCQ3 | |||||
SMRi | Q8VCQ3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 565935, 1 interactor |
IntActi | Q8VCQ3, 8 interactors |
STRINGi | 10090.ENSMUSP00000097254 |
PTM databases
iPTMneti | Q8VCQ3 |
PhosphoSitePlusi | Q8VCQ3 |
Proteomic databases
EPDi | Q8VCQ3 |
MaxQBi | Q8VCQ3 |
PaxDbi | Q8VCQ3 |
PeptideAtlasi | Q8VCQ3 |
PRIDEi | Q8VCQ3 |
ProteomicsDBi | 293970 |
Protocols and materials databases
Antibodypediai | 28311, 302 antibodies from 30 providers |
DNASUi | 641340 |
Genome annotation databases
Ensembli | ENSMUST00000077839; ENSMUSP00000097254; ENSMUSG00000075000 |
GeneIDi | 641340 |
KEGGi | mmu:641340 |
UCSCi | uc007flw.1, mouse |
Organism-specific databases
CTDi | 29982 |
MGIi | MGI:1354950, Nrbf2 |
VEuPathDBi | HostDB:ENSMUSG00000075000 |
Phylogenomic databases
eggNOGi | ENOG502SGH4, Eukaryota |
GeneTreei | ENSGT00390000000984 |
HOGENOMi | CLU_098323_0_0_1 |
InParanoidi | Q8VCQ3 |
OMAi | CEAMKLT |
OrthoDBi | 1613014at2759 |
PhylomeDBi | Q8VCQ3 |
TreeFami | TF328627 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 641340, 11 hits in 72 CRISPR screens |
ChiTaRSi | Nrbf2, mouse |
EvolutionaryTracei | Q8VCQ3 |
PROi | PR:Q8VCQ3 |
RNActi | Q8VCQ3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000075000, Expressed in liver and 74 other tissues |
Genevisiblei | Q8VCQ3, MM |
Family and domain databases
IDEALi | IID50293 |
InterProi | View protein in InterPro IPR039679, NRBF2 IPR015056, NRBF2_C IPR033393, NRBF2_MIT |
PANTHERi | PTHR14964, PTHR14964, 1 hit |
Pfami | View protein in Pfam PF08961, NRBF2, 1 hit PF17169, NRBF2_MIT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NRBF2_MOUSE | |
Accessioni | Q8VCQ3Primary (citable) accession number: Q8VCQ3 Secondary accession number(s): Q9DCG3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references