Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nicalin

Gene

Ncln

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.By similarity

GO - Biological processi

Protein family/group databases

MEROPSiM28.978

Names & Taxonomyi

Protein namesi
Recommended name:
Nicalin
Alternative name(s):
Nicastrin-like protein
Gene namesi
Name:Ncln
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1926081 Ncln

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini43 – 522LumenalSequence analysisAdd BLAST480
Transmembranei523 – 543HelicalSequence analysisAdd BLAST21
Topological domaini544 – 563CytoplasmicSequence analysisAdd BLAST20

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000001968843 – 563NicalinAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi428N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8VCM8
MaxQBiQ8VCM8
PaxDbiQ8VCM8
PeptideAtlasiQ8VCM8
PRIDEiQ8VCM8

PTM databases

iPTMnetiQ8VCM8
PhosphoSitePlusiQ8VCM8
SwissPalmiQ8VCM8

Expressioni

Gene expression databases

BgeeiENSMUSG00000020238 Expressed in 252 organ(s), highest expression level in dorsal pancreas
CleanExiMM_NCLN
ExpressionAtlasiQ8VCM8 baseline and differential
GenevisibleiQ8VCM8 MM

Interactioni

Subunit structurei

Forms a complex with NOMO and TMEM147, resulting in a stabilization of the 3 proteins, which are otherwise quickly degraded by the proteasome. Participates in a large protein complex, which is not related to the gamma-secretase complex (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8VCM8, 4 interactors
MINTiQ8VCM8
STRINGi10090.ENSMUSP00000020463

Structurei

3D structure databases

ProteinModelPortaliQ8VCM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nicastrin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2526 Eukaryota
ENOG410XQZK LUCA
GeneTreeiENSGT00500000044945
HOGENOMiHOG000006315
HOVERGENiHBG054932
InParanoidiQ8VCM8
OrthoDBiEOG091G041D
PhylomeDBiQ8VCM8
TreeFamiTF105849

Family and domain databases

InterProiView protein in InterPro
IPR016574 Nicalin
IPR007484 Peptidase_M28
PANTHERiPTHR31826 PTHR31826, 1 hit
PfamiView protein in Pfam
PF04389 Peptidase_M28, 1 hit
PIRSFiPIRSF011018 Nicalin, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8VCM8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLEEAGEVLE NVLKASCLPL GFIVFLPAVL LLVAPPLPAA DAAHEFTVYR
60 70 80 90 100
MQQYDLQGQP YGTRNAVLNT EARTVDADVL SRRCVLMRLL DFSYEHYQKA
110 120 130 140 150
LRQSAGAVVI ILPRAMAAVP QDVVRQFMEI EPEMLAMETV VPVYFAVEDE
160 170 180 190 200
ALLSIYEQTQ AASASQGSAS AAEVLLHTAT ANGFQMVTSG AQSQAVSDWL
210 220 230 240 250
ITSVEGRLTG LGGEDLPTIV IVAHYDAFGV APWLSLGADS NGSGISVLLE
260 270 280 290 300
LARLFSRLYT YKRTHAAYNL LFFASGGGKF NYQGTKRWLE DSLDHTDSSL
310 320 330 340 350
LQDNVAFVLC LDTVGRGSHL RLHVSKPPRE GTLQHAFLRE LETVAAHQFP
360 370 380 390 400
DVSFSMVHKK INLADDVLAW EHERFAIRRL PAFTLSHLES HRAGPRSSIM
410 420 430 440 450
DVRSRVDSKT LTRNTRIIAE ALTRVIYNLT EKGTPPDMPV FTEQMQVQEE
460 470 480 490 500
QIDSVMDWLT NQPRAAQLLD KDGTFLSTLE HFLSRYLKDV RQHHVKADKR
510 520 530 540 550
DPEFVFYDQL KQVMNAYRVK PAIFDLLLAL CIGAYLGMAY TAVQHFHVLY
560
KTVQRLLLKA KAQ
Length:563
Mass (Da):62,908
Last modified:March 1, 2004 - v2
Checksum:i92F8919DDA48D458
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YU17D3YU17_MOUSE
Nicalin
Ncln
562Annotation score:
F6S731F6S731_MOUSE
Nicalin
Ncln
64Annotation score:

Sequence cautioni

The sequence BAB31708 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131E → D in BAB31708 (PubMed:16141072).Curated1
Sequence conflicti512Q → K in BAB31708 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019409 mRNA Translation: BAB31708.1 Different initiation.
AK048954 mRNA Translation: BAC33498.1
BC019501 mRNA Translation: AAH19501.2
CCDSiCCDS36000.1
RefSeqiNP_598770.1, NM_134009.3
UniGeneiMm.302791

Genome annotation databases

EnsembliENSMUST00000020463; ENSMUSP00000020463; ENSMUSG00000020238
GeneIDi103425
KEGGimmu:103425
UCSCiuc007gig.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019409 mRNA Translation: BAB31708.1 Different initiation.
AK048954 mRNA Translation: BAC33498.1
BC019501 mRNA Translation: AAH19501.2
CCDSiCCDS36000.1
RefSeqiNP_598770.1, NM_134009.3
UniGeneiMm.302791

3D structure databases

ProteinModelPortaliQ8VCM8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCM8, 4 interactors
MINTiQ8VCM8
STRINGi10090.ENSMUSP00000020463

Protein family/group databases

MEROPSiM28.978

PTM databases

iPTMnetiQ8VCM8
PhosphoSitePlusiQ8VCM8
SwissPalmiQ8VCM8

Proteomic databases

EPDiQ8VCM8
MaxQBiQ8VCM8
PaxDbiQ8VCM8
PeptideAtlasiQ8VCM8
PRIDEiQ8VCM8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020463; ENSMUSP00000020463; ENSMUSG00000020238
GeneIDi103425
KEGGimmu:103425
UCSCiuc007gig.1 mouse

Organism-specific databases

CTDi56926
MGIiMGI:1926081 Ncln

Phylogenomic databases

eggNOGiKOG2526 Eukaryota
ENOG410XQZK LUCA
GeneTreeiENSGT00500000044945
HOGENOMiHOG000006315
HOVERGENiHBG054932
InParanoidiQ8VCM8
OrthoDBiEOG091G041D
PhylomeDBiQ8VCM8
TreeFamiTF105849

Miscellaneous databases

PROiPR:Q8VCM8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020238 Expressed in 252 organ(s), highest expression level in dorsal pancreas
CleanExiMM_NCLN
ExpressionAtlasiQ8VCM8 baseline and differential
GenevisibleiQ8VCM8 MM

Family and domain databases

InterProiView protein in InterPro
IPR016574 Nicalin
IPR007484 Peptidase_M28
PANTHERiPTHR31826 PTHR31826, 1 hit
PfamiView protein in Pfam
PF04389 Peptidase_M28, 1 hit
PIRSFiPIRSF011018 Nicalin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCLN_MOUSE
AccessioniPrimary (citable) accession number: Q8VCM8
Secondary accession number(s): Q8C7Y4, Q9CX81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2004
Last modified: November 7, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again