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UniProtKB - Q8VCB3 (GYS2_MOUSE)
Protein
Glycogen [starch] synthase, liver
Gene
Gys2
Organism
Mus musculus (Mouse)
Status
Functioni
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Catalytic activityi
- EC:2.4.1.111 Publication
Activity regulationi
Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does (By similarity).By similarity
Kineticsi
- KM=0.48 mM for UDP-glucose (at 30 degrees Celsius)1 Publication
: glycogen biosynthesis Pathwayi
This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 40 | UDP-glucoseBy similarity | 1 |
GO - Molecular functioni
- glucose binding Source: MGI
- glycogen (starch) synthase activity Source: UniProtKB
GO - Biological processi
- glycogen biosynthetic process Source: UniProtKB
- glycogen metabolic process Source: MGI
- response to glucose Source: UniProtKB
Keywordsi
Molecular function | Allosteric enzyme, Glycosyltransferase, Transferase |
Biological process | Glycogen biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00164 |
Protein family/group databases
CAZyi | GT3, Glycosyltransferase Family 3 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Gys2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2385254, Gys2 |
VEuPathDBi | HostDB:ENSMUSG00000030244 |
Subcellular locationi
Cytoskeleton
- cortical actin cytoskeleton Source: UniProtKB
- cytoskeleton Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Other locations
- cell cortex Source: UniProtKB
- cytoplasm Source: UniProtKB
- ectoplasm Source: UniProtKB
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 8 | S → A: Abolishes phosphorylation. No effect on the interaction with GYG1. Does not affect the result of other mutations; when associated with A-135; R-135; R-141; A-239; A-243; or R-243. 1 Publication | 1 | |
Mutagenesisi | 135 | W → A: No effect on the interaction with GYG1. No effect on the interaction with GYG1; when associated with A-8. 1 Publication | 1 | |
Mutagenesisi | 135 | W → R: Loss of interaction with GYG1. Loss of interaction with GYG1; when associated with A-8. 1 Publication | 1 | |
Mutagenesisi | 141 | G → R: Loss of interaction with GYG1. Loss of function. Loss of interaction with GYG1; when associated with A-8. 1 Publication | 1 | |
Mutagenesisi | 239 | Y → A: Loss of interaction with GYG1. Loss of function. Loss of interaction with GYG1; when associated with A-8. 1 Publication | 1 | |
Mutagenesisi | 243 | C → A: No effect on the interaction with GYG1. Loss of interaction with GYG1; when associated with A-8. 1 Publication | 1 | |
Mutagenesisi | 243 | C → R: Loss of interaction with GYG1. Loss of function. Loss of interaction with GYG1; when associated with A-8. 1 Publication | 1 | |
Mutagenesisi | 510 | E → A: Loss of catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274489 | 1 – 704 | Glycogen [starch] synthase, liverAdd BLAST | 704 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | Phosphoserine; by AMPK and PKABy similarity | 1 | |
Modified residuei | 11 | PhosphoserineCombined sources | 1 | |
Modified residuei | 627 | PhosphoserineBy similarity | 1 | |
Modified residuei | 641 | Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity | 1 | |
Modified residuei | 645 | Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity | 1 | |
Modified residuei | 649 | Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity | 1 | |
Modified residuei | 653 | Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity | 1 | |
Modified residuei | 657 | Phosphoserine; by CK2By similarity | 1 | |
Modified residuei | 684 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme (By similarity). Phosphorylation at Ser-8 is not required for interaction with GYG1 (PubMed:24982189). Interaction with GYG1 does not regulate the phosphorylation at Ser-8 and Ser-641 (PubMed:24982189).By similarity1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8VCB3 |
MaxQBi | Q8VCB3 |
PaxDbi | Q8VCB3 |
PeptideAtlasi | Q8VCB3 |
PRIDEi | Q8VCB3 |
ProteomicsDBi | 271353 |
PTM databases
iPTMneti | Q8VCB3 |
PhosphoSitePlusi | Q8VCB3 |
SwissPalmi | Q8VCB3 |
Expressioni
Inductioni
Expression in the liver oscillates in a circadian manner with peak levels during the night.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000030244, Expressed in liver and 87 other tissues |
ExpressionAtlasi | Q8VCB3, baseline and differential |
Genevisiblei | Q8VCB3, MM |
Interactioni
Subunit structurei
Interacts with GYG1 (via C-terminus); required for GYS2-mediated glycogen synthesis.
1 PublicationProtein-protein interaction databases
IntActi | Q8VCB3, 1 interactor |
STRINGi | 10090.ENSMUSP00000032371 |
Miscellaneous databases
RNActi | Q8VCB3, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 620 – 704 | DisorderedSequence analysisAdd BLAST | 85 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 657 – 673 | Acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 690 – 704 | Basic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the glycosyltransferase 3 family.Curated
Phylogenomic databases
eggNOGi | KOG3742, Eukaryota |
GeneTreei | ENSGT00390000018612 |
HOGENOMi | CLU_015910_1_0_1 |
InParanoidi | Q8VCB3 |
OMAi | DVTIMKR |
OrthoDBi | 264593at2759 |
PhylomeDBi | Q8VCB3 |
TreeFami | TF300306 |
Family and domain databases
InterProi | View protein in InterPro IPR008631, Glycogen_synth |
PANTHERi | PTHR10176, PTHR10176, 1 hit |
Pfami | View protein in Pfam PF05693, Glycogen_syn, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8VCB3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRGRSLSVT SLGGLPVWEA ERLPVEDLLL FEVSWEVTNK VGGICTVIQT
60 70 80 90 100
KAKTTADEWG ENYFLIGPYF EHNMKTQVEQ CEPTNDAVRK AVDAMNKHGC
110 120 130 140 150
QVHFGRWLIE GSPYVVLFDI SSSAWNLDRW KGDFWEACGV GIPHHDREAN
160 170 180 190 200
DMLIFGSLTA WFLKEVTDHA DGKHVIAQFH EWQAGTGLIL SRARKLPIAT
210 220 230 240 250
VFTTHATLLG RYLCAANIDF YNQLDKFDID KEAGERQIYH RYCMERASVH
260 270 280 290 300
CAHVFTTVSE ITAIEAEHML KRKPDVVTPN GLNVKKFSAV HEFQNLHAMY
310 320 330 340 350
KARIQDFVRG HFYGHLDFDL EKTLFLFIAG RYEFSNKGAD IFLESLSRLN
360 370 380 390 400
FLLRMHKSNV TVVVFFIMPA KTNNFNVETL KGQAVRKQLW DTVHCLKEKF
410 420 430 440 450
GKKLYDGLLR GEIPDMNSIL DRDDLTIMKR AIFSTQRQSL PPVTTHNMID
460 470 480 490 500
DSTDPILSTI RRIGLFNNRA DRVKVILHPE FLSSTSPLLP MDYEEFVRGC
510 520 530 540 550
HLGVFPSYYE PWGYTPAECT VMGIPSVTTN LSGFGCFVQE HVADPTAYGI
560 570 580 590 600
YIVDRRFRSP DDSCNQLTQF LYGFCKQSRR QRIIQRNRTE RLSDLLDWRY
610 620 630 640 650
LGRYYQHARH LTLSRAFPDK FHLEPTSPPT TDGFKYPRPS SVPPSPSGSQ
660 670 680 690 700
ASSPQCSDAE DEEDEDERYD EEEEAERDRL NIKSPFSLNH FPKGKKKLHG
EYKN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SVR4 | A0A0N4SVR4_MOUSE | Glycogen [starch] synthase, liver | Gys2 | 34 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 630 | T → M in AAH21322 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 656 | C → S in AAH21322 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK138992 mRNA Translation: BAE23850.1 BC021322 mRNA Translation: AAH21322.1 BC158081 mRNA Translation: AAI58082.1 |
CCDSi | CCDS20683.1 |
RefSeqi | NP_663547.2, NM_145572.2 |
Genome annotation databases
Ensembli | ENSMUST00000032371; ENSMUSP00000032371; ENSMUSG00000030244 |
GeneIDi | 232493 |
KEGGi | mmu:232493 |
UCSCi | uc009epi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK138992 mRNA Translation: BAE23850.1 BC021322 mRNA Translation: AAH21322.1 BC158081 mRNA Translation: AAI58082.1 |
CCDSi | CCDS20683.1 |
RefSeqi | NP_663547.2, NM_145572.2 |
3D structure databases
SMRi | Q8VCB3 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8VCB3, 1 interactor |
STRINGi | 10090.ENSMUSP00000032371 |
Protein family/group databases
CAZyi | GT3, Glycosyltransferase Family 3 |
PTM databases
iPTMneti | Q8VCB3 |
PhosphoSitePlusi | Q8VCB3 |
SwissPalmi | Q8VCB3 |
Proteomic databases
jPOSTi | Q8VCB3 |
MaxQBi | Q8VCB3 |
PaxDbi | Q8VCB3 |
PeptideAtlasi | Q8VCB3 |
PRIDEi | Q8VCB3 |
ProteomicsDBi | 271353 |
Protocols and materials databases
Antibodypediai | 24071, 105 antibodies from 25 providers |
DNASUi | 232493 |
Genome annotation databases
Ensembli | ENSMUST00000032371; ENSMUSP00000032371; ENSMUSG00000030244 |
GeneIDi | 232493 |
KEGGi | mmu:232493 |
UCSCi | uc009epi.2, mouse |
Organism-specific databases
CTDi | 2998 |
MGIi | MGI:2385254, Gys2 |
VEuPathDBi | HostDB:ENSMUSG00000030244 |
Phylogenomic databases
eggNOGi | KOG3742, Eukaryota |
GeneTreei | ENSGT00390000018612 |
HOGENOMi | CLU_015910_1_0_1 |
InParanoidi | Q8VCB3 |
OMAi | DVTIMKR |
OrthoDBi | 264593at2759 |
PhylomeDBi | Q8VCB3 |
TreeFami | TF300306 |
Enzyme and pathway databases
UniPathwayi | UPA00164 |
Miscellaneous databases
BioGRID-ORCSi | 232493, 1 hit in 65 CRISPR screens |
PROi | PR:Q8VCB3 |
RNActi | Q8VCB3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030244, Expressed in liver and 87 other tissues |
ExpressionAtlasi | Q8VCB3, baseline and differential |
Genevisiblei | Q8VCB3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR008631, Glycogen_synth |
PANTHERi | PTHR10176, PTHR10176, 1 hit |
Pfami | View protein in Pfam PF05693, Glycogen_syn, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GYS2_MOUSE | |
Accessioni | Q8VCB3Primary (citable) accession number: Q8VCB3 Secondary accession number(s): Q3UTY0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families